Abstract: The effects of commercial or bovine yeasts on the
performance and blood variables of broiler chickens intoxicated with
aflatoxin were investigated in broilers. Four hundred eighty broilers
(Arbor Acres; 3-wk-old) were randomly assigned to 4 groups. Each
group (120 broiler chickens) was further randomly divided into 6
replicates of 20 chickens. The treatments were control diet without
additives (treatment 1), 250 ppb AFB1 (treatment 2), commercial
yeast, Saccharomyces cerevisiae, (CY 2.5 x 107 CFU/g) + 250 ppb
AFB1 (treatment 3) and bovine yeast, Saccharomyces cerevisiae,
(BY 2.5 x 107 CFU/g + 250 ppb AFB1 (treatment 4). Complete
randomized design (CRD) was used in the experiment. Feed
consumption and body weight were recorded at every five-day
period. On day 42, carcass compositions were determined from 30
birds per treatment. While chicks were sacrificed, 3-4 ml blood
sample was taken and stored frozen at (-20°C) for serum chemical
analysis to determine effects of consumption of diets on blood
chemistry (total protein, albumin, glucose, urea, cholesterol and
triglycerides). There were no significant differences in ADFI among
the treatments(P>0.05). However, BWG, FCR and mortality were
highly significantly different (P
Abstract: In Indonesia, goat milk is often consumed and
believed as anti-allergy. The objective of this research was to study
the effect of goat milk and their fractions (casein and whey)
supplementation on total serum IgE concentrations and leukocytes
count in rat sensitized with contact allergen dinitrochlorobenzene
(DNCB). Female Wistar rats 6-8 weeks old were divided into four
groups: 1) whey, 2) casein, 3) whole milk supplementation and 4)
phosphate-buffered saline/PBS (control). The results showed that
supplementation of goat milk on rats did not affects on total serum
IgE concentrations and number of leukocytes. After sensitized with
DNCB, the monocyte percentage in rats was higher (P
Abstract: This experiment was performed with the purpose of
investigating effect of additional blend of probiotics Saccharomyces
cerevisiae and Lactobacillus acidophilus on plasma fatty acid profiles
particularly conjugated linoleic acid (CLA) in growing goats fed corn
silage, and selected the optimal levels of the probiotics for further study.
Twenty-four growing crossbred (Thai native x Anglo-Nubian) goats that
weighed (14.2 ± 2.3) kg, aged about 6 months, were purchased and
allocated to 4 treatments according to Randomized Complete Block
Design (RCBD) with 6 goats in each treatment. The blocks were made by
weight into heavy, medium, and light goats and each of the treatments
contained two goats from each of the blocks. In the mean time, ruminal
average pH unaffected, but the NH3-N and also plasma urea nitrogen
(p0.05) were raised, but propionic
proportion (p0.05) were reduced in
concurrent with raise of acetic proportion and resultantly C2:C3 ratio
(p>0.05). On plasma fatty acid profiles, total saturated fatty acids
(p>0.05) was increased, and contrasted with decrease of C15:0
(p0.05), and C18-C22 polyunsaturated fatty acids
(p
Abstract: This study was conducted to investigate the optimum
levels of glutamine (Gln) supplementation in broiler diets. A total of
32 one-day-old male chicks with initial body weight 41.5 g were
segregated into 4 groups (8 chicks per group) and subsequently
distributed to individual cages. Feed and water were provided ad
libitum for 21 days. Four dietary treatments were as follows: control
and supplemented Gln at 1, 2 and 3%, respectively. The results found
that the addition Gln had no negative effects on dry matter, organic
matter, ash digestibility or nitrogen retention. Birds fed with 1% Gln
had significantly higher villi wide and villi height : crypt depth ratio
in duodenum than the control chicks and 2 and 3% Gln chicks. It is
suggested that the addition of Gln at 1% indicated a beneficial effect
on improving small intestinal morphology, in addition Gln may
stimulate immune organ development of broiler chickens.
Abstract: Supplementation of palm vitamin E has been reported
to prevent loss of bone density in ovariectomised female rats. The
mechanism by which palm vitamin E exerts these effects is still
unknown. We hypothesized that palm vitamin E may act by
preventing the protein expression changes. Two dimensional poly
acyrilamide gel electrophoresis (2-D PAGE) and PD Quest software
genomic solutions Investigator (proteomics) was used to analyze the
differential protein expression profile in femoral and humeri bones
harvested from three groups of rats; sham-operated rats (SO),
ovariectomised rats (Ovx) and ovariectomised rats supplemented for
2 months with palm vitamin E. The results showed that there were
over 300 valued spot on each of the groups PVE and OVX as
compared to about 200 in SO. Comparison between the differential
protein expression between OVX and PVE groups showed that ten
spots were down –regulated in OVX but up-regulated in PVE. The
ten differential spots were separately named P1-P10. The
identification and understanding of the pathway of the differential
protein expression among the groups is ongoing and may account for
the molecular mechanism through which palm vitamin E exert its
anti-osteoporotic effect.
Abstract: This experiment was conducted to investigate the
effect of dietary supplementation of different levels of black seed
(Nigella sativa L.) on the performance and immune response of broiler chicks. A total 240 day-old broiler chicks were used and
randomly allotted equally into six experimental groups designated as 1, 2, 3, 4, 5 and 6 having black seed at the rate of 0, 2, 4, 6, 8 and
10 g /kg diet respectively. The study was lasted for 42 days. Average body weight, weight gain, relative growth rate, feed
conversion, antibody titer against Newcastle disease, phagocytic activity and phagocytic index, some blood parameters(GOT, GPT,
Glucose, Cholesterol, Triglyceride, Total protein, Albumen, WBCs,
RBCs, Hb and PCV), dressing percentage, weight of different body
organs, abdominal fat weight, were determined. It was found that, N. Sativa significantly improved final body weight, total body gain
and feed conversion ratio of groups 2 and 3 when compared with the control group. Higher levels of N. Sativa did not improve
growth performance of the chicks. Non significant differences were
observed for antibody titer against Newcastle virus, WBCs count,
serum GOT, glucose level, dressing %, relative liver, spleen, heart and head percentages. Lymphoid organs (Bursa and Thymus)
improved significantly with increasing N. Sativa level in all supplemented groups. Serum cholesterol, triglyceride and visible fat
% significantly decreased with Nigella sativa supplementation while
serum GPT level significantly increased with nigella sativa
supplementation.
Abstract: Twenty four New Zealand white rabbits (12 does and
12 bucks) and twenty four Flanders (12 does and 12 bucks) rabbits,
allotted into two feeding regime (6 for each breed, 3 males and 3
females) first one fed commercial ration and second one fed
commercial diet plus sodium butyrate (300 g/ton). The obtained
results showed that at end of 8th week experimental period New
Zealand white rabbits were heavier body weight than Flanders rabbits
(1934.55+39.05 vs. 1802.5+30.99 g); significantly high body weight
gain during experimental period especially during 8th week
(136.1+3.5 vs. 126.8+1.8 g/week); better feed conversion ratio during
all weeks of experiment from first week (3.07+0.16 vs. 3.12+0.10)
till the 8th week of experiment (5.54+0.16 vs. 5.76+0.07) with
significantly high dressing percentages (0.54+0.01 vs. 0.52+0.01).
Also all carcass cuts were significantly high in New Zealand white
rabbits than Flanders. Females rabbits (at the same age) were lower
body weight than males from start of experiment (941.1+39.8
vs.972.1+33.5 g) till the end of experiment (1833.64+37.69 vs.
1903.41+36.93 g); gained less during all weeks of experiment except
during 8th week (132.1+2.3 vs. 130.9+3.4 g/week), with lower
dressing percentage (0.52+0.01 vs. 0.53+0.01) and lighter carcass
cuts than males, however, they had better feed conversion ratio
during 1st week, 7th week and 8th week of experiment. Addition of
300g sodium butyrate/ton of rabbit increased the body weight of
rabbits at the end of experimental period (1882.71+26.45 vs.
1851.5+49.82 g); improve body weight gain at 3rd, 4th, 5th, 6th and
7th week of experiment and significantly improve feed conversion
ratio during all weeks of the experiment from 1st week (2.85+0.07
vs. 3.30+0.15) till the 8th week of the experiment (5.51+0.12 vs.
5.77+0.12). Also the dressing percentage was higher in Sodium
butyrate fed groups than control one (0.53+0.01 vs. 0.52+0.01) and
the most important results of feeding sodium butyrate is the reducing
of the mortality percentage in rabbits during 8 week experiment to
zero percentage as compared with 16% in control group.
Abstract: The effect of phosphorus supplementation of ammoniated rice straw was studied. The in vitro experiment was carried out following the first stage of Tilley and Terry method. The treatments consisting of four diets were A = 50% ammoniated rice straw + 50% concentrate (control), B = A + 0.2% Phosphor (P) supplement, C = A + 0.4% Phosphor (P) supplement, and D = A + 0.6% Phosphor (P) supplement of dry matter. Completely randomized design was used as the experimental design with differences among treatment means were examined using Duncan multiple range test. Variables measured were total bacterial and cellulolytic bacterial population, cellulolytic enzyme activity, ammonia (NH3) and volatile fatty acid (VFA) concentrations, as fermentability indicators and synthesized microbial protein, as well as degradability indicators including dry matter (DM), organic matter (OM), neutral detergent fibre (NDF), acid detergent fibre (ADF) and cellulose. The results indicated that fermentability and degradability of diets consisting ammoniated rice straw with P supplementation were significantly higher than the control diet (P< 0.05). It is concluded that P supplementation is important to improve fermentability and degradability of rations containing ammoniated RS and concentrate. In terms of the most effective level of P supplementation occurred at a supplementation rate of 0.4% of dry matter.
Abstract: The aim of this study was to investigate the effects of
supplementing the diluent of roosters' semen with different levels of
olive oil on motility, viability, morphology and acrosome integrity of
chicken spermatozoa after in vitro storage for up to 72 h. Semen was
collected from 60 White Layer males (62 wk of age) kept in
separated floor pens and randomly divided into six treatment groups
(10 males in each group). Experimental groups were as follows: T1
:fresh semen, T2 : semen extended 1:1 with Al – Daraji 2 diluent
(AD2D) alone, T3 – T6 :semen samples extended 1:1 with AD2D
supplemented with 2 ml, 4 ml, 6 ml or 8 ml of olive oil / 100 ml of
diluent, respectively. Semen samples were then stored at 5 °C for 24
h, 48 h or 72 h. There was a clear influence of diluent
supplementation with olive oil on the spermatozoa motility profile;
olive oil groups (T3, T4, T5 and T6) recorded the highest scores of
mass activity and individual motility during all storage periods
compared to T1 and T2 groups. In addition, the inclusion of olive oil
into semen diluent (T3, T4, T5 and T6) gave significantly higher
percentages of viable spermatozoa, normal morphologically
spermatozoa and intact acrosomes irrespective of storage period.
These results clearly show that supplementation the diluent of
roosters' semen with olive oil can improve semen quality when
semen samples in vitro stored at 5 °C for up to 72 h.
Abstract: A thirty Rahmani weaned male lambs of average body weight (27.28±1.40 kg) were randomly allotted to three similar groups, ten lambs in each, to study the benefit of commercial feed additives Tonilisat (Saccharomyces cerevisiae) and Roemin W2 (Lactobacillus acidophilus, Lactobacillus thermophilus, Bifidobacterium and Lactose) as growth promoters on lambs performance, digestibility, rumen activity and some blood constituents. The experiment lasted about 107 days. Three experimental groups were allotted as control group: received the basal ration, T1 group: received the basal ration supplemented with Tonilisat as (0.5kg/ ton concentrate feed mixture) and T2 group: received the basal ration supplemented with Roemin W2 (1kg/ ton concentrate feed mixture). Our study revealed that addition of Tonilisat significantly increased digestion coefficient of crude protein than that of the control group, Furthermore, the supplementation of Tonilisat or Roemin W2 increased (p
Abstract: The cDNA encoding the 326 amino acids of a Class I
basic chitinase gene from Leucaena leucocephala de Wit (KB3,
Genbank accession: AAM49597) was cloned under the control of
CaMV35S promoter in pCAMBIA 1300 and transferred to
Koshihikari. Calli of Koshihikari rice was transformed with
agrobacterium with this construct expressing the chitinase and β-
glucouronidase (GUS). The frequencies of calli 90 % has been
obtained from rice seedlings cultured on NB medium. The high
regeneration frequencies, 74% was obtained from calli cultured on
regeneration medium containing 4 mg/l BAP, and 7 g/l phytagel at
25°C. Various factors were studied in order to establish a procedure
for the transformation of Koshihikari Agrobacterium tumefaciens.
Supplementation of 50 mM acetosyringone to the medium during
coculivation was important to enhance the frequency to transient
transformation. The 4 week-old scutellum-derived calli were
excellent starting materials. Selection medium based on NB medium
supplement with 40 mg/l hygromycin and 400 mg/l cefotaxime were
an optimized medium for selection of transformed rice calli. The
percentage of transformation 70 was obtained. Recombinant calli and
regenerated rice plants were checked the expression of chitinase and
gus by PCR, northern blot gel, southern blot gel, and gus assay.
Chitinase and gus were expressed in all parts of recombinant rice.
The rice line expressing the KB3 chiitnase was more resistant to the
blast fungus Fusarium monoliforme than control line.
Abstract: The effects of chitosan, a biodegradable polymer,
were studied in Grammatophyllum speciosum protocorm-like bodies
(PLBs) in vitro culture. The chitosan concentration of 0, 5, 10, 15,
20, 25, 50 or 100 mg/l were supplemented in half-strength Murashige
and Skoog (1/2 MS) liquid or on agar media containing 2% (w/v)
sucrose. The results showed that liquid medium supplemented with
15 mg/l chitosan showed the highest relative growth rate (7-fold
increase) of PLBs. On 1/2 MS agar medium supplemented with 25
mg/l chitosan gave the highest relative growth rate (4-fold increase).
The relative growth rate of G. speciosum PLBs on agar medium was
significantly lower than that in liquid medium. Moreover, chitosan,
supplemented to agar medium promoted shoot formation but not
rooting. However, supplementation at too high a level, such as 100
mg/l can inhibit growth and kill PLBs.
Abstract: This study was designed to determine effect of
supplemented tomato pomace and fobrolytic enzyme on egg
production and egg quality. A total of 40 CP brown laying hens (95
week old) were used in completely randomized design in 2x2
factorial arrangement with or without enzyme supplementation. Four
dietary treatments included: Control (C), Fibrolytic enzyme (FE),
10% Tomato pomace (TP), and Fibrolytic enzyme + 10 % Tomato
pomace (FE+TP). Each of the four dietary treatments was fed up to
30 days (10 birds/treatment). Live performance, egg production, egg
weight and quality were determined for whole period. Dietary
treatments had no effect (P>0.05) on live performance, egg weight,
yolk color, and egg production. Therefore, laying hens fed diets with
fibrolytic enzyme were significantly (P
Abstract: Sorghum flour was supplemented with 15 and 30%
chickpea flour. Sorghum flour and the supplement were fermented at
35 oC for 0, 8, 16, and 24 h. Changes in pH, titrable acidity, total
soluble solids, protein content, in vitro protein digestibility and
amino acid composition were investigated during fermentation and/or
after supplementation of sorghum flour with chickpea. The pH of the
fermenting material decreased sharply with a concomitant increase in
the titrable acidity. The total soluble solids remained unchanged with
progressive fermentation time. The protein content of sorghum
cultivar was found to be 9.27 and that of chickpea was 22.47%. The
protein content of sorghum cultivar after supplementation with15 and
30% chickpea was significantly (P ≤ 0.05) increased to 11.78 and
14.55%, respectively. The protein digestibility also increased after
fermentation from 13.35 to 30.59 and 40.56% for the supplements,
respectively. Further increment in protein content and digestibility
was observed when supplemented and unsupplemented samples were
fermented for different periods of time. Cooking of fermented
samples was found to increase the protein content slightly and
decreased digestibility for both supplements. Amino acid content of
fermented and fermented and cooked supplements was determined.
Supplementation was found to increase the lysine and therionine
content. Cooking following fermentation decreased lysine,
isoleucine, valine and sulfur containg amino acids.
Abstract: A feeding trial was conducted to investigate the effect
of periodically use of garlic on performance and carcass
characteristics in broiler chickens. 240 1-day-old Ross broiler chicks
randomly allocated into the 10 dietary treatments (A, B, C, D, E, F,
G, H, I and J) for 6 wk. Treatment A or control group, received basal
diet (based on standards of Ross management guidelines) without
supplementation of garlic powder while B, C and D dietary
treatments were basal diet supplemented with 0.5, 1 and 3% garlic
powder, respectively for the whole time of experiment (6 weeks).
Birds in group E, F and G were fed control diet supplemented with
0.5, 1 and 3% garlic powder, respectively just in their starter diet (0-
21d). Birds in three other treatments (H, I and J) received control diet
for the first 21 days and 0.5, 1 and 3% of garlic powder was added to
their finisher diets, respectively. 1 and 3% supplemented groups in
finisher period had better performance as compared with other
groups. Since present study conducted in optimum and antiseptic
conditions, it seems that better or more responses could be expected
in performance if the raising conditions would not be healthy.
Abstract: Biodiesel production results in glycerol production as
the main by-product in biodiesel industry.One of the utilizations of
glycerol obtained from biodiesel production is as a cement grinding
aid (CGA). Results showed that crude glycerol content was 40.19%
whereas pure glycerol content was 82.15%. BSS value of the cement
with CGA supplementation was higher than that of nonsupplemented
cement (blank) indicating that CGA-supplemented
cement had higher fineness than the non-supplemented one. It was
also found that pure glycerol 95% and TEA 5% at 80ºC was the
optimum CGA used to result in finest cement with BSS value of
4.836 cm2/g. Residue test showed that the smallest percent residue
value (0.11%) was obtained in cement with supplementation of pure
glycerol 95% and TEA 5%. Results of residue test confirmed those of
BSS test showing that cement with supplementation of pure glycerol
95% and TEA 5% had the finest particle size.
Abstract: A study was conducted to determine the effect of
feeding glycerol on dairy cows performance. Twenty four Holstein
Friesian crossbred (>87.5% Holstein Friesian) lactating dairy cows in
early lactation; averaging 13+2.4 kg of milk, 64+45 days in milk,
55+16 months old and 325+26 kg live weight, were stratified for
milk yield, days in milk, age, stage of lactation and body weight, and
then randomly allocated to three treatment groups. All cows were fed
approximate 8 kg of concentrate together with ad libitum corn silage
and freely access to clean water. Nil or 150 and 300g of glycerol
were supplemented to the cows according to treatment groups. All
cows consumed similar concentrate, corn silage and total DM and
NELP. There were no significant differences in DM intake, CP intake,
NELP intake, milk and milk composition yields. All cows had similar
fat, protein, lactose, solid not fat and total solid percentage. All cows
gain similar live weight. The present study indicated that,
supplementation of glycerol did not enhance milk yield, milk
composition and live weight change.
Abstract: Three batches of yogurts were made with soy protein
isolate (SPI) supplemented with 2% (S2), 4% (S4) or 6% (S6) of
skim milk powder (SMP). The fourth batch (control; S0) was
prepared from SPI without SMP supplementation. Lactobacillus
delbrueckii ssp. bulgaricus ATCC 11842 (Lb 11842) and
Streptococcus thermophilus ST 1342 (ST 1342) were used as the
starter culture. Biotransformation of the inactive forms, isoflavone
glycosides (IG) to biologically active forms, isoflavone aglycones
(IA), was determined during 28 d storage. The viability of both
microorganisms was significantly higher (P < 0.05) in S2, S4, and S6
than that in S0. The ratio of lactic acid/acetic acid in S0 was in the
range of 15.53 – 22.31 compared to 7.24 – 12.81 in S2, S4 and S6.
The biotransformation of IG to IA in S2, S4 and S6 was also
enhanced by 9.9 -13.3% compared to S0.
Abstract: The aim of current study was to investigate the
changes in the quality parameters of Holstein bull semen during the
heat stress and the effect of feeding a source of omega-3 fatty acids
in this period. Samples were obtained from 19 Holstein bulls during
the expected time of heat stress in Iran (June to September 2009).
Control group (n=10) were fed a standard concentrate feed while
treatment group (n=9) had this feed top dressed with 100 g of an
omega-3 enriched nutriceutical. Semen quality was assessed on
ejaculates collected after 1, 5, 9 and 12 weeks of supplementation.
Computer-assisted assessment of sperm motility, viability (eosinnigrosin)
and hypo-osmotic swelling test (HOST) were conducted.
Heat stress affected sperm quality parameters by week 5 and 9
(p
Abstract: Xanthan gum is a microbial polysaccharide of great
commercial significance. The purpose of this study was to select the
optimum fermentation time for xanthan gum production by
Xanthomonas campestris (NRRL-B-1459) using 10% sugar beet
molasses as a carbon source. The pre-heating of sugar beet molasses
and the supplementation of the medium were investigated in order to
improve xanthan gum production. Maximum xanthan gum
production in fermentation media (9.02 g/l) was observed after 4 days
shaking incubation at 25°C and 240 rpm agitation speed. A solution
of 10% sucrose was used as a control medium. Results indicated that
the optimum period for xanthan gum production in this condition was
4 days.