Abstract: This first-attempt study revealed that decolorized
intermediates of azo dyes could act as redox mediators to assist
wastewater (WW) decolorization due to enhancement of
electron-transport phenomena. Electrochemical impedance spectra
indicated that hydroxyl and amino-substituent(s) were functional
group(s) as redox-mediator(s). As azo dyes are usually multiple
benzene-rings structured, their derived decolorized intermediates are
likely to play roles of electron shuttles due to lower barrier of energy
gap for electron shuttling. According to cyclic voltammetric profiles,
redox mediating characteristics of decolorized intermediates of azo
dyes (e.g., RBu171, RR198, RR141, RBk5) were clearly disclosed.
With supplementation of biodecolorized metabolites of RR141 and
198, decolorization performance of could be evidently augmented.
This study also suggested the optimal modes of microbial fuel cell
(MFC)-assisted WW decolorization would be plug-flow or batch
mode of operation with no mix. Single chamber-MFCs would be more
favourable than double chamber MFCs due to non-mixing contacting
reactor scheme for operation.
Abstract: The aim of the study was to investigate the effect of
Saccharomyces cerevisiae (SC) live yeast culture on microbial
protein supply to small intestine in Kivircik male yearlings when fed
with different ratio of forage and concentrate diets. Four Kivircik
male yearlings with permanent rumen canula were used in the
experiment. The treatments were allocated to a 4x4 Latin square
design. Diet I consisted of 70% alfalfa hay and 30% concentrate, Diet
II consisted of 30% alfalfa hay and 70% concentrate, Diet I and II
were supplemented with a SC. Daily urine was collected and stored at
-20°C until analysis. Calorimetric methods were used for the
determination of urinary allantoin and creatinine levels. The
estimated microbial N supply to small intestine for Diets I, I+SC, II
and II+SC were 2.51, 2.64, 2.95 and 3.43 g N/d respectively.
Supplementation of Diets I and II with SC significantly affected the
allantoin levels in μmol/W0.75 (p
Abstract: Effects of nicotine on pre-partum body weight and
preimplantation embryonic development has been reported
previously. Present study was conducted to determine the effects of
annatto (Bixa orellana)-derived delta-tocotrienol (TCT) (with
presence of 10% gamma-TCT isomer) on the nicotine-induced
reduction in body weight and 8-cell embryonic growth in mice.
Twenty-four 6-8 weeks old (23-25g) female balb/c mice were
randomly divided into four groups (G1-G4; n=6). Those groups were
subjected to the following treatments for 7 consecutive days: G1
(control) were gavaged with 0.1 ml tocopherol stripped corn oil. G2
was subcutaneously (s.c.) injected with 3 mg/kg/day of nicotine. G3
received concurrent treatment of nicotine (3 mg/kg/day) and 60
mg/kg/day of δ-TCT mixture (contains 90% delta & 10% gamma
isomers) and G4 was given 60 mg/kg/day of δ-TCT mixture alone.
Body weights were recorded daily during the treatment. On Day 8,
females were superovulated with 5 IU Pregnant Mare’s Serum
Gonadotropin (PMSG) for 48 hours followed with 5 IU human
Chorionic Gonadotropin (hCG) before mated with males at the ratio
of 1:1. Females were sacrificed by cervical dislocation for embryo
collection 48 hours post-coitum. Collected embryos were cultured in
vitro. Results showed that throughout Day 1 to Day 7, the body
weight of nicotine treated group (G2) was significantly lower
(p
Abstract: Several embryonic cellular mechanism including cell
cycle, growth and apoptosis are regulated by phosphatidylinositol-3-
kinase (PI3K)/Akt signaling pathway. The goal of present study is to
determine the effects of annatto (Bixa orellana)-derived δ-tocotrienol
(δ-TCT) on the regulations of PI3K/Akt genes in murine morula.
Twenty four 6-8 week old (23-25g) female balb/c mice were
randomly divided into four groups (G1-G4; n=6). Those groups were
subjected to the following treatments for 7 consecutive days: G1
(control) received tocopherol stripped corn oil, G2 was given 60
mg/kg/day of δ-TCT mixture (contains 90% delta & 10% gamma
isomers), G3 was given 60 mg/kg/day of pure δ-TCT (>98% purity)
and G4 received 60 mg/kg/day α-TOC. On Day 8, females were
superovulated with 5 IU Pregnant Mare’s Serum Gonadotropin
(PMSG) for 48 hours followed with 5 IU human Chorionic
Gonadotropin (hCG) before mated with males at the ratio of 1:1.
Females were sacrificed by cervical dislocation for embryo collection
48 hours post-coitum. About fifty morulas from each group were
used in the gene expression analyses using Affymetrix QuantiGene
Plex 2.0 Assay. Present data showed a significant increase (p
Abstract: An experiment was conducted to elucidate the effects of turmeric powder supplementation on egg production performance of old laying hens (80 weeks of age). There were 40 hens of Hysex Brown strain used in the study. They were caged individually, and randomly divided into 4 treatment groups of diet containing 0 (control), 1, 2 and 4 % oven dried turmeric powder for 3 periods of 4 weeks; Egg production (% hen day) and feed intake of the 4 treatment groups at the commencement of the experiment were not significantly different. In addition to egg production performance (% and egg weight), feed and water intakes were measured daily, and cholesterol content of the whole egg was determined. The results indicated that feed intakes of the hen were significantly lowered when 4% turmeric powder supplemented, while there were no significant changes in water intakes. Egg production were significantly increased and maintained at a higher level by turmeric powder supplementation up to 4% compared with the control, while the weight of eggs were not significantly affected. The research markedly demonstrated that supplementation of turmeric powder up to 4% could improve and maintain egg production performance of the old laying hen at a higher level with a lower cholesterol content.
Abstract: Current research is targeting new molecular
mechanisms that underlie non-alcoholic fatty liver disease (NAFLD)
and associated metabolic disorders like non-alcoholic steatohepatitis
(NASH). Forty New Zealand White rabbits have been used and fed a
high protein (HP) and energy diet based on grains and containing
11.76 MJ/kg. Boron added to 3 experimental groups’ drinking waters
(30 mg boron/L) as boron compounds. Biochemical analysis
including boron levels, and nuclear magnetic resonance (NMR) based
metabolomics evaluation, and mRNA expression of peroxisome
proliferator-activated receptor (PPAR) family was performed. LDLcholesterol
concentrations alone were decreased in all the
experimental groups. Boron levels in serum and feces were increased.
Content of acetate was in about 2x higher for anhydrous borax group,
at least 3x higher for boric acid group. PPARα mRNA expression
was significantly decreased in boric acid group. Anhydrous borax
attenuated mRNA levels of PPARγ, which was further suppressed by
boric acid. Boron supplementation decreased the degenerative
alterations in hepatocytes. Except borax group other boron groups did
not have a pronounced change in tubular epithels of kidney. In
conclusion, high protein and energy diet leads hepatocytes’
degenerative changes which can be prevented by boron
supplementation. Boric acid seems to be more effective in this
situation.
Abstract: The effects of basil and/or chamomile seed
supplementation on the growth of Hubbard broiler chicks were
evaluated. The antioxidant effects of these supplements were also
assessed. 120 1-day-old broiler chicks were randomly divided into
four equal groups. The control group (group 1) was fed a basal diet
(BD) without supplementation. Groups 2, 3, and 4 were fed the BD
supplemented with 10g basil, 10g chamomile, and 5g basil plus 5g
chamomile per kg of food, respectively. Basil supplementation alone
or in combination with chamomile non-significantly (P≥0.05)
increased final body weight (3.2% and 0.3%, respectively) and
weight gain (3.5% and 3.6%, respectively) over the experimental
period. Chamomile supplementation alone non-significantly (P≥0.05)
reduced final body weight and weight gain over the experimental
period by 1.7% and 1.7%, respectively. In comparison to the control
group, herbal seed supplementation reduced feed intake and
improved the feed conversion and protein efficiency ratios. In
general, basil seed supplementation stimulated chicken growth and
improved the feed efficiency more effectively than chamomile seed
supplementation. The antioxidant activities of basil and/or chamomile
supplementation were examined in the thymus, bursa, and spleen. In
chickens that received supplements, the level of malondialdehyde
was significantly decreased, whereas the activities of glutathione,
superoxide dismutase, and catalase were significantly increased
(P
Abstract: A collection of thirty cultivars/clones of a red pitaya
was used to investigate flowering response to lighting
supplementation in the winter season of 2013-2014 in southern
Taiwan. The night-breaking treatment was conducted during the
period of 10 Oct. 2013 to 5 Mar. 2014 with 4-continuous hours
(22.00 – 02.00 hrs) of additional lighting daily using incandescent
bulbs (100W). Among cultivars and clones tested, twenty-three
genotypes, most belonging to the red-magenta flesh type, were found
to have positively flowering response to the lighting treatment. The
duration of night-breaking treatment for successful flowering
initiation varied from 33- 48 days. The lighting-sensitive genotypes
bore 1-2 flowering flushes. Floral and fruiting stages took 21-26 and
46-59 days, respectively. Among sixteen fruiting genotypes, the
highest fruit set rates were found in Damao 9, D4, D13, Chaozou
large, Chaozhou 5, Small Nick and F22. Five cultivars and clones
(Orejona, D4, Chaozhou large, Chaozhou 5 and Small Nick) produced
fruits with an average weight of more than 300 g per fruit which were
higher than those of the fruits formed in the summer of 2013. Fruits
produced during off-season containing total soluble solids (TSS)
from 17.5 to 20.7oBrix, which were higher than those produced inseason.
Abstract: The objective of this study was to determine effect of
dietary essential oil (EO) compounds, which contained
cinnamaldehyde, eugenol, peppermint, coriander, cumin, lemongrass,
and an organic carrier on feed intake, milk composition, and rumen
fermentation of dairy cows during heat exposure. Thirty-two Holstein
cows (days in milk= 60 ± 5) were assigned to one of two treatment
groups: a Control and EO fed. The experiment lasted 28 days. Dry
matter intake (DMI) was measured daily while and milk production
was measured weekly. Our result showed that DMI and milk yield
was decreased (P < 0.01) in control cows relative to EO cows.
Furthermore, supplementation with EO was associated with a
decrease in the molar proportion of propionate (P < 0.05) and
increase (P < 0.05) in acetate to propionate ratio. In conclusion, EO
supplementations in diets can be useful nutritional modification to
alleviate for the decrease DMI and milk production during heat
exposure in lactating dairy cows.
Abstract: The main goal of this paper was evaluate the effect of
diets containing different levels of probiotic on performance and milk
composition of lactating cows.
Eight Holstein cows were distributed in two 4x4 Latin square. The
diets were based on corn silage, concentrate and the treatment (0, 3, 6
or 9 grams of probiotic/animal/day). It was evaluated the dry matter
intake of nutrients, milk yield and composition.
The use of probiotics did not affect the nutrient intake (p>0.05)
neither the daily milk production or corrected to 4% fat (p>0.05).
However, it was observed that there was a significant fall in milk
composition with higher levels of probiotics supplementation.
These results emphasize the need of further studies with different
experimental designs or improve the number of Latin square with
longer periods of adaptation.
Abstract: This study was aimed to investigate the effect of
various organic supplements on growth and development of
Dendrobium discolor’s protocorms and seedlings growth of
Dendrobium Judy Rutz. Protocorms of Dendrobium discolor with 2.0
cm. in diameter and seedlings of Dendrobium Judy Rutz at the same
size (0.5 cm. height) were sub-cultured on Hyponex medium
supplemented with cow milk (CM), soy milk (SM), potato extract
(PE) and peptone (P) for 2 months. The protocorms were developed
to seedlings in all treatments after cultured for 2 months. However,
the best results were found on Hyponex medium supplemented with
P was the best in which the maximum fresh and dry weight and
maximum shoot height were obtained in this treatment statistically
different (p ≤ 0.05) to other treatments. Moreover, Hyponex medium
supplemented with P also stimulated the maximum mean number of
5.7 shoots per explant which also showed statistically different (p ≤
0.05) when compared to other treatments. The results of growth of
Dendrobium Judy Rutz seedlings indicated the medium
supplemented with 100 mL/L PE enhanced the maximum fresh and
dry weigh per explants with significantly different (p ≤ 0.05) in fresh
weight from other treatments including the control medium without
any organic supplementation. However, the dry weight was not
significantly different (p ≤ 0.05) from medium supplemented with
SM and P. There was multiple shoots induction in all media with or
without organic supplementation ranging from 2.6 to 3 shoots per
explants. The maximum shoot height was also obtained in the
seedlings cultured on medium supplemented with PE while the
longest root length was found in medium supplemented with SM.
Abstract: This study examined the effects of zinc (Zn) from
different sources and microbial phytase on the broiler performance,
biochemical parameters and digestibility of nutrients when they were
added to broiler diets containing low available phosphorus. A total of
875, 1-day-old male broilers of the Ross 308 strain were randomly
separated into two control groups (positive and negative) and five
treatment groups each containing 125 birds; each group was divided
into 5 replicates of 25 birds. The positive control (PC) group was fed
a diet containing adequate concentration (0.45%) of available
phosphorus due to mineral premix (except zinc) and feeds. The
negative control (NC) group was fed a basal diet including low
concentration (0.30%) of available phosphorus due to mineral premix
(except zinc) and feeds. The basal diet was supplemented with 0.30%
phosphorus and 500 FTU phytase (PH); 0.30% phosphorus and
organic zinc (OZ; 75mg/kg of Zn from Zn-proteinate); 0.30%
phosphorus and inorganic zinc (IZ; 75 mg/kg of Zn from ZnSO4);
0.30% phosphorus, organic zinc and 500 FTU phytase (OZ + PH);
and 0.30% phosphorus, inorganic zinc and 500 FTU phytase (IZ +
PH) in the treatment groups 1, 2, 3, 4 and 5, respectively. The lowest
value for mean body weight was in the negative control group on a
diet containing low available phosphorus. The use of
supplementation with organic and inorganic zinc alone or in
combination with microbial phytase significantly (P
Abstract: In mammalian reproductive tract, the oviduct secretes
huge number of growth factors and cytokines that create an optimal
micro-environment for the initial stages of preimplantation embryos.
Secretion of these growth factors is stage-specific. Among them,
VEGF is a potent mitogen for vascular endothelium and stimulates
vascular permeability. Apart from angiogenesis, VEGF in the oviduct
may be involved in regulating the oocyte maturation and subsequent
developmental process during embryo production in vitro. In
experiment 1, to evaluate the effect of VEGF during IVM of porcine
COC and subsequent developmental ability after PA and SCNT. The
results from these experiments indicated that maturation rates among
the different VEGF concentrations were not significant different. In
experiment 2, total intracellular GSH concentrations of oocytes
matured with VEGF (5-50 ng/ml) were increased significantly
compared to a control and VEGF group (500 ng/ml). In experiment 3,
the blastocyst formation rates and total cell number per blastocyst
after parthenogenesis of oocytes matured with VEGF (5-50 ng/ml)
were increased significantly compared to a control and VEGF group
(500 ng/ml). Similarly, in experiment 4, the blastocyst formation rate
and total cell number per blastocyst after SCNT and IVF of oocytes
matured with VEGF (5 ng/ml) were significantly higher than that of
oocytes matured without VEGF group. In experiment 5, at 10 hour
after the onset of IVF, pronuclear formation rate was evaluated.
Monospermy was significantly higher in VEGF-matured oocytes than
in the control, and polyspermy and sperm penetration per oocyte
were significantly higher in the control group than in the VEGFmatured
oocytes. Supplementation with VEGF during IVM
significantly improved male pronuclear formation as compared with
the control. In experiment 6, type III cortical granule distribution in
oocytes was more common in VEGF-matured oocytes than in the
control. In conclusion, the present study suggested that
supplementation of VEGF during IVM may enhance the
developmental potential of porcine in vitro embryos through increase
of the intracellular GSH level, higher MPN formation and increased
fertilization rate as a consequence of an improved cytoplasmic
maturation.
Abstract: The objective of this study was to investigate the effects of dietary supplementation with raw or heat-treated sunflower oil seed with two levels of 7.5% or 15% on unsaturated fatty acids in milk fat and performances of high-yielding lactating cows. Twenty early lactating Holstein cows were used in a complete randomized design. Treatments included: 1) CON, control (without sunflower oil seed). 2) LS-UT, 7.5% raw sunflower oil seed. 3) LS-HT, 7.5% heat-treated sunflower oil seed. 4) HS-UT, 15% raw sunflower oil seed. 5) HS-HT, 15% heat-treated sunflower oil seed. Experimental period lasted for 4 wk, with first 2 wk used for adaptation to the diets. Supplementation with 7.5% raw sunflower seed (LS-UT) tended to decrease milk yield, with 28.37 kg/d compared with the control (34.75 kg/d). Milk fat percentage was increased with the HS-UT treatment that obtained 3.71% compared with CON that was 3.39% and without significant different. Milk protein percent was decreased high level sunflower oil seed treatments (15%) with 3.18% whereas CON treatment is caused 3.40% protein. The cows fed added low sunflower heat-treated (LS-HT) produced milk with the highest content of total unsaturated fatty acid with 32.59 g/100g of milk fat compared with the HS-UT with 23.59 g/100g of milk fat. Content of C18 unsaturated fatty acids in milk fat increased from 21.68 g/100g of fat in the HS-UT to 22.50, 23.98, 27.39 and 30.30 g/100g of fat from the cow fed HS-HT, CON, LS-UT and LS-HT treatments, respectively. C18:2 isomers of fatty acid in milk were greater by LSHT supplementation with significant effect (P < 0.05). Total of C18 unsaturated fatty acids content was significantly higher in milk of animal fed added low heat-treated sunflower (7.5%) than those fed with high sunflower. In all, results of this study showed that diet cow's supplementation with sunflower oil seed tended to reduce milk production of lactating cows but can improve C18 UFA (Unsaturated Fatty Acid) content in milk fat. 7.5% level of sunflower oil seed that heated seemed to be the optimal source to increase UFA production.
Abstract: Oxidative stress and overwhelming free radicals
associated with diabetes mellitus are likely to be linked with
development of certain complication such as retinopathy,
nephropathy and neuropathy. Treatment of diabetic subjects with
antioxidant may be of advantage in attenuating these complications.
Olive leaf (Oleaeuropaea), has been endowed with many beneficial
and health promoting properties mostly linked to its antioxidant
activity. This study aimed to evaluate the significance of
supplementation of Olive leaves extract (OLE) in reducing oxidative
stress, hyperglycemia and hyperlipidemia in Sterptozotocin (STZ)-
induced diabetic rats. After induction of diabetes, a significant rise in
plasma glucose, lipid profiles except High density lipoproteincholestrol
(HDLc), malondialdehyde (MDA) and significant decrease
of plasma insulin, HDLc and Plasma reduced glutathione GSH as
well as alteration in enzymatic antioxidants was observed in all
diabetic animals. During treatment of diabetic rats with 0.5g/kg body
weight of Olive leaves extract (OLE) the levels of plasma (MDA)
,(GSH), insulin, lipid profiles along with blood glucose and
erythrocyte enzymatic antioxidant enzymes were significantly
restored to establish values that were not different from normal
control rats. Untreated diabetic rats on the other hand demonstrated
persistent alterations in the oxidative stress marker (MDA), blood
glucose, insulin, lipid profiles and the antioxidant parameters. These
results demonstrate that OLE may be of advantage in inhibiting
hyperglycemia, hyperlipidemia and oxidative stress induced by
diabetes and suggest that administration of OLE may be helpful in
the prevention or at least reduced of diabetic complications
associated with oxidative stress.
Abstract: Vitamin A deficiency is a public health problem in
Zimbabwe. Addressing vitamin A deficiency has the potential of
enhancing resistance to disease and reducing mortality especially in
children less than 5 years. We implemented and adapted vitamin A
outreach supplementation strategy within the National Immunization
Days and Extended Programme of Immunization in a rural district in
Zimbabwe. Despite usual operational challenges faced this approach
enabled the district to increase delivery of supplementation coverage.
This paper describes the outreach strategy that was implemented in
the remote rural district. The strategy covered 63 outreach sites with
2 sites being covered per day and visited once per month for the
whole year. Coverage reached 71% in an area of previous coverage
rates of around less than 50%. We recommend further exploration of
this strategy by others working in similar circumstances. This
strategy can be a potential way for use by Scaling-Up-Nutrition
member states.
Abstract: Our objectives were to evaluate the effects of sire
breed, type of protein supplement, level of supplementation and sex
on wool spinning fineness (SF), its correlations with other wool
characteristics and prediction accuracy in F1 Merino crossbred lambs.
Texel, Coopworth, White Suffolk, East Friesian and Dorset rams
were mated with 500 purebred Merino dams at a ratio of 1:100 in
separate paddocks within a single management system. The F1
progeny were raised on ryegrass pasture until weaning, before forty
lambs were randomly allocated to treatments in a 5 x 2 x 2 x 2
factorial experimental design representing 5 sire breeds, 2
supplementary feeds (canola or lupins), 2 levels of supplementation
(1% or 2% of liveweight) and sex (wethers or ewes). Lambs were
supplemented for six weeks after an initial three weeks of adjustment,
wool sampled at the commencement and conclusion of the feeding
trial and analyzed for SF, mean fibre diameter (FD), coefficient of
variation (CV), standard deviation, comfort factor (CF), fibre
curvature (CURV), and clean fleece yield. Data were analyzed using
mixed linear model procedures with sire fitted as a random effect,
and sire breed, sex, supplementary feed type, level of
supplementation and their second-order interactions as fixed effects.
Sire breed (P
Abstract: Two group of kids (“Safflower cake" and “Control") were fed ad libitum with pelleted total mixed rations. After a 7-days adaptation period, the diet of the “Safflower cake" group were supplemented with 20% of safflower cake. The kids were slaughtered at 96 days of age. Dietary safflower cake did not affect the growth traits of kids. In addition, kids fed experimental diet showed a lower feed intake and consequently a better feed conversion ratio in comparison to the “Control" group. The use of safflower decreased the level of SFA and increased the level of MUFA in kid meat. The level of PUFA was higher in lipid extracted from animals feeding “Control“ diet even if the UFA level was lower. Furthermore, lipid extracted from animals feeding control diet contained more ω6 fatty acids in comparison to kids feeding experimental diet while the opposite trend was observed for the level of ω3 fatty acids. The ω6 to ω3 ratio was significantly affected by diet and in particular this ratio decreased in meat of kids fed experimental diet. Our results indicate that intramuscular fatty acid composition of kid meat can be improved from a human health perspective by inclusion of safflower cake in the diet.
Abstract: This experiment was conducted to investigate the
effect of different levels of dietary chromium yeast (Cr-yeast) on
thigh meat quality of broiler chicks reared under heat stress
condition. Two hundred and forty Ross male chickens in heat stress
condition (33±3°C) were allocated to five treatments in a completely
randomized design. Treatments were supplemented with 0 (control),
200, 400, 800 and 1200 μg kg-1 Cr in the form of Cr yeast. Twelve
chicks from each treatment were slaughtered at 42 d, to evaluate
moisture, protein, lipid, pH and lipid oxidation of thigh meat.
Protein, moisture, lipid and pH of thigh meat were not affected by
supplemental Cr. Thigh meat lipid tended to decrease in broilers
received 1200 μg kg-1. Storage time increased lipid oxidation of
meat (P
Abstract: Dietary macro and micro nutrients in their respective proportion and fractions present a practical potential tool to fabricate milk constituents since cells of lactating mammary glands obtain about 80 % of milk synthesis nutrients from blood, reflecting the existence of an isotonic equilibrium between blood and milk. Diverting milk biosynthetic activities through manipulation of nutrients towards producing milk not only keeping in view its significance as natural food but also as food item which prevents or dilutes the adverse effects of some diseases (like cardiovascular problem by saturated milk fat intake) has been area of interest in the last decade. Nutritional modification / supplementation has been reported to enhance conjugated linoleic acid, fatty acid type and concentration, essential fatty acid concentration, vitamin B12& C, Se, Cu, I and Fe which are involved to counter the health threats to human well being. Synchronizing dietary nutrients aimed to modify rumen dynamics towards synthesis of nutrients or their precursors to make their drive towards formulated milk constituents presents a practical option. Formulating dietary constituents to design milk constituents will let the farmers, consumers and investors know about the real potential and profit margins associated with this enterprise. This article briefly recapitulates the ways and means to modify milk constituents keeping an eye on human health and well being issues, which allows milk to serve more than a food item.