Abstract: A study was carried out to evaluate the growth and yield performance of Pleurotus ostreatus spawn on different organic substrates in Lafia, Nasarawa State, Nigeria. 50 g each of four different substrates namely; corncobs, rice straw, sugarcane bagasse and sawdust sourced locally from farmlands and processing sites, were amended with 2% calcium carbonate and calcium sulphide and sterilized using three sterilization methods namely; hot water, steam, and lime. Five grams of P. ostreatus spawn were inoculated unto treated substrates, incubated in the dark for 16 days and in light for 19 days at 25 0C for the commencement of pinhead and fruit body formations respectively. Growth and yield parameters such as days to full colonization, days to pinhead formation and days to fruit body formation were recorded. Cap diameter and fresh weight of mature mushrooms were also measured for a total count of four flushes. P. ostreatus spawn grown on sugarcane bagasse recorded the highest mean cap diameter (4.69 cm), highest mean fresh weight (34.68 g), highest biological efficiency (69.37%) and highest production rate (2.83 g per day). Spawn grown on rice straw recorded the least number of days to full substrate colonization (11.00). Spawn grown on corn cobs recorded the least mean number of days to pin head (18.75) and fruiting body formations (20.25). There were no significant differences (P ≤ 0.05) among the evaluated substrates with respect to growth and yield performance of P. ostreatus. Substrates sterilized with hot water supported the highest mean cap diameter (5.64 cm), highest biological efficiency (87.04%) and highest production rate (3.43 g per day) of P. ostreatus. Significant differences (P ≤ 0.05) were observed in cap diameter, fresh weight, biological efficiency and production rates among the evaluated sterilization methods. Hot water sterilization of sugarcane bagasse could be adopted for enhanced yield of oyster mushrooms, especially among indigent farming communities in Nigeria and beyond.
Abstract: This study provides a regeneration protocol for Treculia africana Decne (an endangered plant) through embryo culture. Mature zygotic embryos of T. africana were excised from the seeds aseptically and cultured on varied strengths (full, half and quarter) of Murashige and Skoog (MS) basal medium supplemented. All treatments experienced 100±0.00 percent sprouting except for half and quarter strengths. Plantlets in MS full strength had the highest fresh weight, leaf area, and longest shoot length when compared to other treatments. All explants in full, half, quarter strengths and control had the same number of leaves and sprout rate. Between the treatments, there was a significant difference (P>0.05) in their effect on the length of shoot and root, number of adventitious root, leaf area, and fresh weight. Full strength had the highest mean value in all the above-mentioned parameters and differed significantly (P>0.05) from others except in shoot length, number of adventitious roots, and root length where it did not differ (P
Abstract: Less availability of high quality green forages leads to low productivity of national dairy herd of Sri Lanka. Growing grass and fodder to suit the production system is an efficient and economical solution for this problem. CO-3 is placed in a higher category, especially on tillering capacity, green forage yield, regeneration capacity, leaf to stem ratio, high crude protein content, resistance to pests and diseases and free from adverse factors along with other fodder varieties grown within the country. An experiment was designed to determine the effect of organic sheep manure, inorganic fertilizers and liquid sheep manure on growth, yield and nutritive value of CO-3. The study was consisted with three treatments; sheep manure (T1), recommended inorganic fertilizers (T2) and liquid sheep manure (T3) which was prepared using bucket fermentation method and each treatment was consisted with three replicates and those were assigned randomly. First harvest was obtained after 40 days of plant establishment and number of leaves (NL), leaf area (LA), tillering capacity (TC), fresh weight (FW) and dry weight (DW) were recorded and second harvest was obtained after 30 days of first harvest and same set of data were recorded. SPSS 16 software was used for data analysis. For proximate analysis AOAC, 2000 standard methods were used. Results revealed that the plants treated with T1 recorded highest NL, LA, TC, FW and DW and were statistically significant at first and second harvest of CO-3 (p˂ 0.05) and it was found that T1 was statistically significant from T2 and T3. Although T3 was recorded higher than the T2 in almost all growth parameters; it was not statistically significant (p ˃0.05). In addition, the crude protein content was recorded highest in T1 with the value of 18.33±1.61 and was lowest in T2 with the value of 10.82±1.14 and was statistically significant (p˂ 0.05). Apart from this, other proximate composition crude fiber, crude fat, ash, moisture content and dry matter were not statistically significant between treatments (p ˃0.05). In accordance with the results, it was found that the organic fertilizer is the best fertilizer for CO-3 in terms of growth parameters and crude protein content.
Abstract: Blueberries are widely valued for their high content in
phenolic compounds with antioxidant activity, and hence beneficial
for the human health. In this way, a study was done to determine the
phenolic composition (total phenols, anthocyanins and tannins) and
antioxidant activity of blueberries from three cultivars (Duke,
Bluecrop, and Ozarkblue) grown in two different Portuguese farms.
Initially two successive extractions were done with methanol
followed by two extractions with aqueous acetone solutions. These
extracts obtained were then used to evaluate the amount of phenolic
compounds and the antioxidant activity. The total phenols were
observed to vary from 4.9 to 8.2 mg GAE/g fresh weight, with
anthocyanin’s contents in the range 1.5-2.8 mg EMv3G/g and tannins
contents in the range 1.5- 3.8 mg/g. The results for antioxidant
activity ranged from 9.3 to 23.2 molTE/g and from 24.7 to 53.4molTE/g, when measured, respectively, by DPPH and ABTS
methods. In conclusion it was observed that, in general, the cultivar
had a visible effect on the phenols present, and furthermore, the
geographical origin showed relevance either in the phenols contents
or the antioxidant activity.
Abstract: This study was aimed to investigate the effect of
various organic supplements on growth and development of
Dendrobium discolor’s protocorms and seedlings growth of
Dendrobium Judy Rutz. Protocorms of Dendrobium discolor with 2.0
cm. in diameter and seedlings of Dendrobium Judy Rutz at the same
size (0.5 cm. height) were sub-cultured on Hyponex medium
supplemented with cow milk (CM), soy milk (SM), potato extract
(PE) and peptone (P) for 2 months. The protocorms were developed
to seedlings in all treatments after cultured for 2 months. However,
the best results were found on Hyponex medium supplemented with
P was the best in which the maximum fresh and dry weight and
maximum shoot height were obtained in this treatment statistically
different (p ≤ 0.05) to other treatments. Moreover, Hyponex medium
supplemented with P also stimulated the maximum mean number of
5.7 shoots per explant which also showed statistically different (p ≤
0.05) when compared to other treatments. The results of growth of
Dendrobium Judy Rutz seedlings indicated the medium
supplemented with 100 mL/L PE enhanced the maximum fresh and
dry weigh per explants with significantly different (p ≤ 0.05) in fresh
weight from other treatments including the control medium without
any organic supplementation. However, the dry weight was not
significantly different (p ≤ 0.05) from medium supplemented with
SM and P. There was multiple shoots induction in all media with or
without organic supplementation ranging from 2.6 to 3 shoots per
explants. The maximum shoot height was also obtained in the
seedlings cultured on medium supplemented with PE while the
longest root length was found in medium supplemented with SM.
Abstract: This study aimed to investigate effect of different organic supplements on growth of Vanda and Mokara seedlings. Vanda and Mokara seedlings approximately 0.2 and 0.3 cm. in height were sub-cultured onto VW supplemented with 150 ml/L coconut water, 100 g/L potato extract, 100 g/L ‘Gros Michel’ banana (AAA group) and 100 g/L ‘Namwa’ banana (ABB group). The explants were sub-cultured onto the same medium every month for 3 months. The best medium increased stem height to 0.52 and 0.44 Cm. in Vanda and Mokara respectively was supplemented with coconut water. The maximum fresh weight of Vanda (0.59 g) was found on medium supplemented with ‘Gros Michel’ banana while Mokara cultured on medium supplemented with Potato extract had the maximum fresh weight (0.27 g) and number of roots (5.20 roots/shoot) statistically different (p≤ 0.05) to other treatments. However, Vanda cultured on medium supplemented with ‘Namwa’ banana had the maximum number of roots (3.80 roots/shoot). Our results suggested that growth of different orchid genera was responded diversely to different organic supplements.
Abstract: Grasslands of Iran are encountered with a vast
desertification and destruction. Some legumes are plants of forage
importance with high palatability. Studied legumes in this project are
Onobrychis, Medicago sativa (alfalfa) and Trifolium repens. Seeds
were cultivated in research field of Kaboutarabad (33 km East of
Isfahan, Iran) with an average 80 mm. annual rainfall. Plants were
cultivated in a split plot design with 3 replicate and two water
treatments (weekly irrigation, and under stress with same amount per
15 days interval). Water entrance to each plots were measured by
Partial flow. This project lasted 20 weeks. Destructive samplings
(1m2 each time) were done weekly. At each sampling plants were
gathered and weighed separately for each vegetative parts. An Area
Meter (Vista) was used to measure root surface and leaf area. Total
shoot and root fresh and dry weight, leaf area index and soil coverage
were evaluated too. Dry weight was achieved in 750c oven after 24
hours. Statgraphic and Harvard Graphic software were used to
formulate and demonstrate the parameters curves due to time. Our
results show that Trifolium repens has affected 60 % and Medicago
sativa 18% by water stress. Onobrychis total fresh weight was
reduced 45%. Dry weight or Biomass in alfalfa is not so affected by
water shortage. This means that in alfalfa fields we can decrease the
irrigation amount and have some how same amount of Biomass.
Onobrychis show a drastic decrease in Biomass. The increases in
total dry matter due to time in studied plants are formulated. For
Trifolium repens if removal or cattle entrance to meadows do not
occurred at perfect time, it will decrease the palatability and water
content of the shoots. Water stress in a short period could develop the
root system in Trifolium repens, but if it last more than this other
ecological and soil factors will affect the growth of this plant. Low
level of soil water is not so important for studied legume forges. But
water shortage affect palatability and water content of aerial parts.
Leaf area due to time in studied legumes is formulated. In fact leaf
area is decreased by shortage in available water. Higher leaf area
means higher forage and biomass production. Medicago and
Onobrychis reach to the maximum leaf area sooner than Trifolium
and are able to produce an optimum soil cover and inhibit the
transpiration of soil water of meadows. Correlation of root surface to
Total biomass in studied plants is formulated. Medicago under water
stress show a 40% decrease in crown cover while at optimum
condition this amount reach to 100%. In order to produce forage in
areas without soil erosion Medicago is the best choice even with a
shortage in water resources. It is tried to represent the growth
simulation of three famous Forage Legumes. By growth simulation
farmers and range managers could better decide to choose best plant
adapted to water availability without designing different time and
labor consuming field experiments.
Abstract: Plant tissue culture is an important in vitro technology applied for agricultural and industrial production. A sterile condition of culture medium is one of the main aspects. The alternative technique for medium sterilization to replace autoclaving was carried out. For sterilization of plant tissue culture medium without autoclaving, ten commercial pure essential oils and 5 disinfectants were tested. Each essential oil or disinfectant was added to a 20-mL Murashige and Skoog (MS) medium before medium was solidified in a 120-mL container, kept for 2 weeks before evaluating sterile conditions. Treated media, supplemented with essential oils or disinfectants, were compared to control medium, autoclaved at 121 degree Celsius for 15 min. Sterile conditions of MS medium were found 100% from betel oil or clove oil (18 mL/20 mL medium), cinnamon oil (36 mL/20 mL medium), lavender oil or holy basil oil (108 mL/20 mL medium), and lemon oil or tea tree oil or turmeric oil (252 mL/20 mL medium), compared to 100% sterile condition from autoclaved medium. For disinfectants, 2% iodine + 2.4% potassium iodide, 2% merbromine solution, 10% povidone-iodine, 6% sodium hypochlorite or 0.1% thimerosal at 36 mL/20 mL medium provided 100% sterile conditions. Furthermore, growth of new shoots from chrysanthemum node explants on treated media (fresh weight, shoot length, root length and number of node) were also reported and discussed in the comparison of those on autoclaved medium.
Abstract: Photoselective plastic films with thermic properties
are now available so that greenhouses clad with such plastics exhibit
a higher degree of “Greenhouse Effect” with a consequent increase in
night time temperature. In this study, we investigate the potential
benefits of a range of thermic plastic films used as greenhouse cover
materials on the vegetative and reproductive growth and development
of Iceberg lettuce (Lactuca sativa L). Transplants were grown under
thermic films and destructively harvested 4, 5, and 6 weeks after
transplanting. Thermic films can increase night temperatures up to 2
⁰C reducing the wide fluctuation in greenhouse temperature during
winter compared to the standard commercial film and consequently
increased the yield (leaf number, fresh weight, and dry weight) of
lettuce plants. Lettuce plants grown under Clear film respond to cold
stress by the accumulation of secondary products (phenolics, and
flavonoids).
Abstract: The evaluation of non-conventional water resources
on seed germination and seedling growth performance at early
growth stages is still in progress especially in forage crops. This
study was designed to test the effect of four types of water qualities
(treated wastewater (TWW), industrial water (IW), grey water (GW),
and Distilled water (DW)) on germination and early seedling vigor of
Leucaena leucocephala. The results showed that the germination
was not significantly affected by the different water qualities. Seed
germination reached maximum after 17, 14, 14, and 21 days under
GW, IW, TWW, and DW treatments, respectively. The highest mean
of shoot length was scored under the GW treatment. And, the highest
mean of root length was scored under DW which was not significant
from GW treatment. The means of shoot fresh was the highest under
the TWW. The means of root fresh weight was not significantly
different from each other's under different treatments. The growth
performance was in progress with no mortality during 21 days of
growth. Thus, the best non-conventional water qualities alternatives
based on the cleanness, nutrients, and toxicity are the GW, TWW and
IW, respectively.
Abstract: For sterilization of Phalaenopsis culture medium without autoclaving, selected single sterilizing agents and in combinations were added to a 25ml Hyponex medium in a 120ml glass container. Treated liquid and solid media, supplemented with sterilizing agents, were compared to a control medium, autoclaved at 121°C for 15min. It was found that 90(L of 10% povidone-iodine, 150(L of 5.25% sodium hypochlorite, 150(L of 2% mercurochrome, 90(L of 2.5% iodine + 2.5% potassium iodine in combination with 10% providone-iodine (1:3) and 30(L of 2.5% iodine + 2.5% potassium iodide in combination with 2% mercurochrome showed 100% sterile conditions in liquid medium but provided 75, 100, 50, 75 and 80% sterile conditions, respectively, in solid medium. For growth of Phalaenopsis protocorms, 90(L of 10% povidone-iodine in liquid Hyponex medium gave the comparable growth of protocorms to control medium while 150(L of 5.25% sodium hypochlorite in solid medium provided the promising growth of protocorms. Growth of protocorms, whole fresh weight, numbers of leaf and root, root length and number of protocorm-like bodies, was discussed.
Abstract: Salinity level may affect early development of
biofuel feedstock crops. The biofuel feedstock crops canola
(Brassica napus L.), sorghum [Sorghum bicolor (L.) Moench], and
sunflower (Helianthus annuus L.); and the potential feedstock crop
sweet corn (Zea mays L.) were planted in media in pots and treated
with aqueous solutions of 0, 0.1, 0.5 and 1.0 M NaCl once at: 1)
planting; 2) 7-10 days after planting or 3) first true leaf expansion.
An additional treatment (4) comprised of one-half strength of the 0.1,
0.5 and 1.0 M (concentrations 0.05, 0.25, 0.5 M at each application)
was applied at first true leaf expansion and four days later. Survival
of most crops decreased below 90% above 0.5 M; survival of canola
decreased above 0.1 M. Application timing had little effect on crop
survival. For canola root fresh and dry weights improved when
application was at plant emergence; for sorghum top and root fresh
weights improved when the split application was used. When
application was at planting root dry weight was improved over most
other applications. Sunflower top fresh weight was among the
highest when saline solutions were split and top dry weight was
among the highest when application was at plant emergence. Sweet
corn root fresh weight was improved when the split application was
used or application was at planting. Sweet corn root dry weight was
highest when application was at planting or plant emergence. Even at
high salinity rates survival rates greater than what might be expected
occurred. Plants that survived appear to be able to adjust to saline
during the early stages of development.