Abstract: Optic disk segmentation plays a key role in the mass
screening of individuals with diabetic retinopathy and glaucoma
ailments. An efficient hardware-based algorithm for optic disk
localization and segmentation would aid for developing an automated
retinal image analysis system for real time applications. Herein,
TMS320C6416DSK DSP board pixel intensity based fractal analysis
algorithm for an automatic localization and segmentation of the optic
disk is reported. The experiment has been performed on color and
fluorescent angiography retinal fundus images. Initially, the images
were pre-processed to reduce the noise and enhance the quality. The
retinal vascular tree of the image was then extracted using canny
edge detection technique. Finally, a pixel intensity based fractal
analysis is performed to segment the optic disk by tracing the origin
of the vascular tree. The proposed method is examined on three
publicly available data sets of the retinal image and also with the data
set obtained from an eye clinic. The average accuracy achieved is
96.2%. To the best of the knowledge, this is the first work reporting
the use of TMS320C6416DSK DSP board and pixel intensity based
fractal analysis algorithm for an automatic localization and
segmentation of the optic disk. This will pave the way for developing
devices for detection of retinal diseases in the future.
Abstract: This study, for its research subjects, uses patients who
had undergone total knee replacement surgery from the database of the
National Health Insurance Administration. Through the review of
literatures and the interviews with physicians, important factors are
selected after careful screening. Then using Cross Entropy Method,
Genetic Algorithm Logistic Regression, and Particle Swarm
Optimization, the weight of each factor is calculated and obtained. In
the meantime, Excel VBA and Case Based Reasoning are combined
and adopted to evaluate the system. Results show no significant
difference found through Genetic Algorithm Logistic Regression and
Particle Swarm Optimization with over 97% accuracy in both
methods. Both ROC areas are above 0.87. This study can provide
critical reference to medical personnel as clinical assessment to
effectively enhance medical care quality and efficiency, prevent
unnecessary waste, and provide practical advantages to resource
allocation to medical institutes.
Abstract: The crude methanol extracts of five indigenous vegetables namely, Amarathus tricolor, Basella rubra L., Chochurus olitorius L., Ipomea batatas, and Momordica chuchinensis L., were examined for their phytochemical profile and antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical. The values for DPPH radical scavenging activity ranged from 7.6-89.53% with B. rubra and I. batatas having the lowest and highest values, respectively. The total flavonoid content of all five indigenous vegetables ranged from 74.65-277.3 mg quercetin equivalent per gram of dried vegetable material while the total phenolic content ranged from 1.93-6.15 mg gallic acid equivalent per gram dried material. Phytochemical screening revealed the presence of steroids, flavonoids, saponins, tannins, carbohydrates and reducing sugars, which may also be associated with the antioxidant activity shown by these indigenous vegetables.
Abstract: Development of new generation bio-tribological,
multilayer coatings opens an avenue for fabrication of future hightech
functional surfaces. In the presented work, nano-composite,
Cr/CrN+[Cr/ a-C:H implanted by metallic nanocrystals] multilayer
coatings have been developed for surface protection of medical tools.
Thin films were fabricated by a hybrid Pulsed Laser Deposition
technique. Complex microstructure analysis of nanomultilayer
coatings, subjected to mechanical and biological tests, were
performed by means of transmission electron microscopy (TEM).
Microstructure characterization revealed the layered arrangement of
Cr23C6 nanoparticles in multilayer structure. Influence of deposition
conditions on bio-tribological properties of the coatings was studied.
The bio-tests were used as a screening tool for the analyzed
nanomultilayer coatings before they could be deposited on medical
tools. Bio-medical tests were done using fibroblasts. The mechanical
properties of the coatings were investigated by means of a ball-ondisc
mechanical test. The micro hardness was done using Berkovich
indenter. The scratch adhesion test was done using Rockwell
indenter. From the bio-tribological point of view, the optimal
properties had the C106_1 material.
Abstract: The paper presents a novel screening method to
indicate congenital heart diseases (CHD), which otherwise could
remain undetected because of their low level. Therefore, not
belonging to the high-risk population, the pregnancies are not subject
to the regular fetal monitoring with ultrasound echocardiography.
Based on the fact that CHD is a morphological defect of the heart
causing turbulent blood flow, the turbulence appears as a murmur,
which can be detected by fetal phonocardiography (fPCG). The
proposed method applies measurements on the maternal abdomen
and from the recorded sound signal a sophisticated processing
determines the fetal heart murmur. The paper describes the problems
and the additional advantages of the fPCG method including the
possibility of measurements at home and its combination with the
prescribed regular cardiotocographic (CTG) monitoring. The
proposed screening process implemented on a telemedicine system
provides an enhanced safety against hidden cardiac diseases.
Abstract: Mammography is widely used technique for breast cancer
screening. There are various other techniques for breast cancer screening
but mammography is the most reliable and effective technique. The
images obtained through mammography are of low contrast which
causes problem for the radiologists to interpret. Hence, a high quality
image is mandatory for the processing of the image for extracting any
kind of information from it. Many contrast enhancement algorithms have
been developed over the years. In the present work, an efficient
morphology based technique is proposed for contrast enhancement of
masses in mammographic images. The proposed method is based on
Multiscale Morphology and it takes into consideration the scale of the
structuring element. The proposed method is compared with other stateof-
the-art techniques. The experimental results show that the proposed
method is better both qualitatively and quantitatively than the other
standard contrast enhancement techniques.
Abstract: Some plants of genus Schinus have been used in the
folk medicine as topical antiseptic, digestive, purgative, diuretic,
analgesic or antidepressant, and also for respiratory and urinary
infections. Chemical composition of essential oils of S. molle and S.
terebinthifolius had been evaluated and presented high variability
according with the part of the plant studied and with the geographic
and climatic regions. The pharmacological properties, namely
antimicrobial, anti-tumoural and anti-inflammatory activities are
conditioned by chemical composition of essential oils. Taking into
account the difficulty to infer the pharmacological properties of
Schinus essential oils without hard experimental approach, this work
will focus on the development of a decision support system, in terms
of its knowledge representation and reasoning procedures, under a
formal framework based on Logic Programming, complemented with
an approach to computing centered on Artificial Neural Networks
and the respective Degree-of-Confidence that one has on such an
occurrence.
Abstract: Medicinal plants are now gaining attractiveness in
treatment of bacterial infections and food preservation. The objective
of this study was to assess antibacterial activity of some medicinal
plants on pathogenic bacteria. Screening of antibacterial activity of
aqueous and methanol extracts of some plants: Jojoba, Ginger, Sage,
Thyme and Clove against Bacillus cereus, Salmonella typhimurium,
Staphylococcus aureus, Clostridium perfringens and Escherichia coli
were investigated. Antibacterial activity was performed by agar
diffusion and disc diffusion method. Jatropha, Jojoba, Clove and
Ginger extracts showed notable bacterial activity in the first
screening step then selected to be tested against Bacillus cereus
(Gram+), Staphylococcus aureus (Gram+) and Salmonella
typhimurium (Gram−) and their effect was compared using
antibiotics as control. Screening results showed potential antibacterial
activity of the tested plant extracts against the screened bacterial
strains. It was found that methanol extracts exhibited higher
antibacterial activity than aqueous extracts. Methanol extract of
Jatropha showed the highest inhibition zone against Staphylococcus
aureus (Gram+) with 24.00 mm diameter, compared to the other
plant extracts followed by clove. Meanwhile, the inhibition zones of
methanol extracts of Jojoba and Ginger were the same (12mm).The
Gram-positive bacteria were found to be more sensitive to aqueous
and methanol extracts than Gram-negative bacteria.
Abstract: Kidney cancer is the most lethal urological cancer
accounting for 3% of adult malignancies. VHL, a tumor-suppressor
gene, is best known to be associated with renal cell carcinoma
(RCC). The VHL functions as negative regulator of hypoxia inducible
factors. Recent sequencing efforts have identified several novel
frequent mutations of histone modifying and chromatin remodeling
genes in ccRCC (clear cell RCC) including PBRM1 and SETD2. The
PBRM1 gene encodes the BAF180 protein, which involved in
transcriptional activation and repression of selected genes. SETD2
encodes a histone methyltransferase, which may play a role in
suppressing tumor development. In this study, RNAs of 30 paired
tumor and normal samples that were grouped according to the types
of kidney cancer and clinical characteristics of patients, including
gender and average age were examined by RT-PCR, SSCP and
sequencing techniques. VHL, PBRM1 and SETD2 expressions were
relatively down-regulated. However, statistically no significance was
found (Wilcoxon signed rank test, p>0.05). Interestingly, no mutation
was observed on the contrary of previous studies. Understanding the
molecular mechanisms involved in the pathogenesis of RCC has
aided the development of molecular-targeted drugs for kidney cancer.
Further analysis is required to identify the responsible genes rather
than VHL, PBRM1 and SETD2 in kidney cancer.
Abstract: This study attempts to identify the factors influencing
on women empowerment of rural area in Sri Lanka through micro
finance services. Data were collected from one hundred (100) rural
women involving self-employment activities through a questionnaire
using direct personal interviews. Judgment and Convenience Random
sampling technique was used to select the sample size from three
Divisional Secretariat divisions of Kandawalai, Poonakari and
Karachchi in Kilinochchi District. The factor analysis was performed
on fourteen (14) variables for screening and reducing the variables to
identify the influencing factors on empowerment. Multiple regression
analysis was used to identify the relationship between the three
empowerment factors and the impact of micro finance on overall
empowerment of rural women. The result of this study summarized
the variables into three factors namely decision making, freedom to
mobility and family support and which are positively associated with
empowerment. In addition to this the value of adjusted R2 is 0.248
indicates that all the variables extracted can be explained 24.8% of
the variation in the women empowerment through microfinance.
Independent variables of these three factors have positive correlation
with women empowerment as well as significant values at 5 percent
level.
Abstract: Breast cancer is considered as a substantial health
concern and practicing mammography screening [MS] is important in
minimizing its related morbidity. So it is essential to have a better
understanding of breast cancer screening behaviors of women and
factors that influence utilization of them. The aim of this study is to
identify the factors that are linked to MS behaviors among the
Egyptian women. A cross-sectional descriptive design was carried
out to provide a snapshot of the factors that are linked to MS
behaviors. A convenience sample of 311 women was utilized and all
eligible participants admitted to the Women Imaging Unit who are 40
years of age or above, coming for mammography assessment, not
pregnant or breast feeding and who accepted to participate in the
study were included. A structured questionnaire was developed by
the researchers and contains three parts; Socio-demographic data;
Motivating factors associated with MS; and association between MS
and model of behavior change. The analyzed data indicated that most
of the participated women (66.6%) belonged to the age group of 40-
49.A high proportion of participants (58.1%) of group having
previous MS influenced by their neighbors to practice MS, whereas
32.7 % in group not having previous MS were influenced by family
members which indicated significant differences (P
Abstract: In this article, the antibiogram and heavy metal
resistance profile of the bacteria isolated from total 34 studied
animals (Pelophylax ridibundus = 12; Mauremys rivulata = 14;
Natrix natrix = 8) captured around the Biga Stream, are described.
There was no database information on antibiogram and heavy metal
resistance profile of bacteria from these area’s amphibians and
reptiles.
A total of 200 bacteria were successfully isolated from cloaca and
oral samples of the aquatic amphibians and reptiles as well as from
the water sample. According to Jaccard’s similarity index, the degree
of similarity in the bacterial flora was quite high among the
amphibian and reptile species under examination, whereas it was
different from the bacterial diversity in the water sample. The most
frequent isolates were A. hydrophila (31.5%), B. pseudomallei
(8.5%), and C. freundii (7%). The total numbers of bacteria obtained
were as follows: 45 in P. ridibundus, 45 in N. natrix 30 in M.
rivulata, and 80 in the water sample. The result showed that
cefmetazole was the most effective antibiotic to control the bacteria
isolated in this study and that approximately 93.33% of the bacterial
isolates were sensitive to this antibiotic. The multiple antibiotic
resistances (MAR) index indicated that P. ridibundus (0.95) > N.
natrix (0.89) > M. rivulata (0.39). Furthermore, all the tested heavy
metals (Pb+2, Cu+2, Cr+3, and Mn+2) inhibit the growth of the bacterial
isolates at different rates. Therefore, it indicated that the water source
of the animals was contaminated with both antibiotic residues and
heavy metals.
Abstract: In our research we aimed to test a managerial
approach for the fuzzy front end (FFE) of innovation by creating
controlled experiment/ business case in a breakthrough innovation
development. The experiment was in the sport industry and covered
all aspects of the customer discovery stage from ideation to
prototyping followed by patent application. In the paper we describe
and analyze mile stones, tasks, management challenges, decisions
made to create the break through innovation, evaluate overall
managerial efficiency that was at the considered FFE stage.
We set managerial outcome of the FFE stage as a valid product
concept in hand. In our paper we introduce hypothetical construct
“Q-factor” that helps us in the experiment to distinguish quality of
FFE outcomes.
The experiment simulated for entrepreneur the FFE of innovation
and put on his shoulders responsibility for the outcome of valid
product concept. While developing managerial approach to reach the
outcome there was a decision to look on product concept from the
cognitive psychology and cognitive science point of view. This view
helped us to develop the profile of a person whose projection (mental
representation) of a new product could optimize for a manager or
entrepreneur FFE activities. In the experiment this profile was tested
to develop breakthrough innovation for swimmers. Following the
managerial approach the product concept was created to help
swimmers to feel/sense water. The working prototype was developed
to estimate the product concept validity and value added effect for
customers.
Based on feedback from coachers and swimmers there were strong
positive effect that gave high value for customers, and for the
experiment – the valid product concept being developed by proposed
managerial approach for the FFE.
In conclusions there is a suggestion of managerial approach that
was derived from experiment.
Abstract: Co-crystal is believed to improve the solubility and
dissolution rates and thus, enhanced the bioavailability of poor water
soluble drugs particularly during the oral route of administration.
With the existing of poorly soluble drugs in pharmaceutical industry,
the screening of co-crystal formation using carbamazepine (CBZ) as
a model drug compound with dicarboxylic acids co-crystal formers
(CCF) namely fumaric (FA) and succinic (SA) acids in ethanol has
been studied. The co-crystal formations were studied by varying the
mol ratio values of CCF to CBZ to access the effect of CCF
concentration on the formation of the co-crystal. Solvent evaporation,
slurry and cooling crystallization which representing the solution
based method co-crystal screening were used. Based on the
differential scanning calorimetry (DSC) analysis, the melting point of
CBZ-SA in different ratio was in the range between 188oC-189oC.
For CBZ-FA form A and CBZ-FA form B the melting point in
different ratio were in the range of 174oC-175oC and 185oC-186oC
respectively. The product crystal from the screening was also
characterized using X-ray powder diffraction (XRPD). The XRPD
pattern profile analysis has shown that the CBZ co-crystals with FA
and SA were successfully formed for all ratios studied. The findings
revealed that CBZ-FA co-crystal were formed in two different
polymorphs. It was found that CBZ-FA form A and form B were
formed from evaporation and slurry crystallization methods
respectively. On the other hand, in cooling crystallization method,
CBZ-FA form A was formed at lower mol ratio of CCF to CBZ and
vice versa. This study disclosed that different methods and mol ratios
during the co-crystal screening can affect the outcome of co-crystal
produced such as polymorphic forms of co-crystal and thereof. Thus,
it was suggested that careful attentions is needed during the screening
since the co-crystal formation is currently one of the promising
approach to be considered in research and development for
pharmaceutical industry to improve the poorly soluble drugs.
Abstract: In review the generalized data about biological
activity of anthraquinone-containing plants and specimens on their
basis is presented. Data of traditional medicine, results of
bioscreening and clinical researches of specimens are analyzed.
Abstract: Fungal mutant strains have produced cellulase and
xylanase enzymes, and have induced high hydrolysis with enhanced
of rice straw. The mutants were obtained by exposing Penicillium
strain to UV-light treatments. Screening and selection after treatment
with UV-light were carried out using cellulolytic and xylanolytic
clear zones method to select the hypercellulolytic and
hyperxylanolytic mutants. These mutants were evaluated for their
cellulase and xylanase enzyme production as well as their abilities for
biodegradation of rice straw. The mutant 12 UV/1 produced 306.21%
and 209.91% cellulase and xylanase, respectively, as compared with
the original wild type strain. This mutant showed high capacity of
rice straw degradation. The effectiveness of tested mutant strain and
that of wild strain was compared in relation to enhancing the
composting process of rice straw and animal manures mixture. The
results obtained showed that the compost product of inoculated
mixture with mutant strain (12 UV/1) was the best compared to the
wild strain and un-inoculated mixture. Analysis of the composted
materials showed that the characteristics of the produced compost
were close to those of the high quality standard compost. The results
obtained in the present work suggest that the combination between
rice straw and animal manure could be used for enhancing the
composting process of rice straw and particularly when applied with
fungal decomposer accelerating the composting process.
Abstract: Content Based Image Retrieval (CBIR) coupled with
Case Based Reasoning (CBR) is a paradigm that is becoming
increasingly popular in the diagnosis and therapy planning of medical
ailments utilizing the digital content of medical images. This paper
presents a survey of some of the promising approaches used in the
detection of abnormalities in retina images as well in
mammographic screening and detection of regions of interest
in MRI scans of the brain. We also describe our proposed
algorithm to detect hard exudates in fundus images of the
retina of Diabetic Retinopathy patients.
Abstract: The rhizome of Java grass, Cyperus rotundus was
extracted different organic polar and non-polar solvents and
performed the in vitro antiviral and immunostimulant activities
against White Spot Syndrome Virus (WSSV) and Vibrio harveyi
respectively. Based on the initial screening the ethyl acetate extract of
C. rotundus was strong activities and further it was purified through
silica column chromatography and the fractions were screened again
for antiviral and immunostimulant activity. Among the different
fractions screened against the WSSV and V. harveyi, the fractions, FIII
to FV had strong activities. In order to study the in vivo influence
of C. rotundus, the fractions (F-III to FV) were pooled and delivered
to the F. indicus through artificial feed for 30 days. After the feeding
trail the experimental and control diet fed F. indicus were challenged
with virulent WSSV and studied the survival, molecular diagnosis,
biochemical, haematological, and immunological parameters.
Surprisingly, the pooled fractions (F-IV to FVI) incorporated diets
helped to significantly (P
Abstract: Agriculture is the backbone of economy of Pakistan
and cotton is the major agricultural export and supreme source of raw
fiber for our textile industry. To combat severe problems of insect
and weed, combination of three genes namely Cry1Ac, Cry2A and
EPSPS genes was transferred in locally cultivated cotton variety
MNH-786 with the use of Agrobacterium mediated genetic
transformation. The present study focused on the molecular screening
of transgenic cotton plants at T3 generation in order to confirm
integration and expression of all three genes (Cry1Ac, Cry2A and
EPSP synthase) into the cotton genome. Initially, glyphosate spray
assay was used for screening of transgenic cotton plants containing
EPSP synthase gene at T3 generation. Transgenic cotton plants which
were healthy and showed no damage on leaves were selected after 07
days of spray. For molecular analysis of transgenic cotton plants in
the laboratory, the genomic DNA of these transgenic cotton plants
were isolated and subjected to amplification of the three genes. Thus,
seventeen out of twenty (Cry1Ac gene), ten out of twenty (Cry2A
gene) and all twenty (EPSP synthase gene) were produced positive
amplification. On the base of PCR amplification, ten transgenic plant
samples were subjected to protein expression analysis through
ELISA. The results showed that eight out of ten plants were actively
expressing the three transgenes. Real-time PCR was also done to
quantify the mRNA expression levels of Cry1Ac and EPSP synthase
gene. Finally, eight plants were confirmed for the presence and active
expression of all three genes at T3 generation.
Abstract: Early detection of breast cancer saves many thousands
of lives each year via application of mammography and genetic
screening and many more lives could be saved if nurses are involved
in breast care screening practices. So, the aim of the study was to
identify nurse's role in early detection of breast cancer through
mammography and genetic screening and its impact on patient's
outcome. In order to achieve this aim, 400 women above 40 years,
asymptomatic were recruited for mammography and genetic
screening. In addition, 50 nurses and 6 technologists were involved in
the study. A descriptive analytical design was used. Five tools were
utilized: sociodemographic, mammographic examination and risk
factors, women's before, during and after mammography, items
relaying to technologists, and items related to nurses were also
obtained. The study finding revealed that 3% of women detected for
malignancy and 7.25% for fibroadenoma. Statistically significant
differences were found between mammography results and age,
family history, genetic screening, exposure to smoke, and using
contraceptive pills. Nurses have insufficient knowledge about
screening tests. Based on these findings the present study
recommended involvement of nurses in breast care which is very
important to in force population about screening practices.