Abstract: Enzymes are the biocatalysts which catalyze the biochemical processes and thus have a wide variety of applications in the industrial sector. β-Galactosidase (E.C. 3.2.1.23) also known as lactase, is one of the prime enzymes, which has significant potential in the dairy and food processing industries. It has the capability to catalyze both the hydrolytic reaction for the production of lactose hydrolyzed milk and transgalactosylation reaction for the synthesis of prebiotics such as lactulose and galactooligosaccharides. These prebiotics have various nutritional and technological benefits. Although, the enzyme is naturally present in almonds, peaches, apricots and other variety of fruits and animals, the extraction of enzyme from these sources increases the cost of enzyme. Therefore, focus has been shifted towards the production of low cost enzyme from the microorganisms such as bacteria, yeast and fungi. As compared to yeast and bacteria, fungal β-galactosidase is generally preferred as being extracellular and thermostable in nature. Keeping the above in view, the present study was carried out for the isolation of the β-galactosidase producing fungal strain from the food as well as the agricultural wastes. A total of more than 100 fungal cultures were examined for their potential in enzyme production. All the fungal strains were screened using X-gal and IPTG as inducers in the modified Czapek Dox Agar medium. Among the various isolated fungal strains, the strain exhibiting the highest enzyme activity was chosen for further phenotypic and genotypic characterization. The strain was identified as Rhizomucor pusillus on the basis of 5.8s RNA gene sequencing data.
Abstract: A teacher’s attitude to creativity plays an essential role in the thinking development of his/her students. The purpose of this study is to understand if a science teacher's personal creativity can modify his/her ability to produce various kinds of questions. This research used an education activity based on cosmic sketches and pictures by K.E. Tsiolkovsky, the founder of astronautics, to explore if any relationship between individual creativity and the asking questions skill exists. As a screening instrument, which allows an assessment of the respondent's creative potential, a common test of creative thinking was used. The results of the creativity test and the diversity of the questions are mentioned.
Abstract: Fly ash-slag based Geopolymer Cement (GPC) is presenting mechanical properties and environmental advantages that make it the predominant “green” alternative to Portland Cement (PC). Although numerous life-cycle analyses praising its environmental advantages, disposal after the end of its life remains as an issue that has been barely explored. The present study is investigating the recyclability of fly ash-slag GPC as aggregate in mortars. The purpose of the study was to evaluate the effect of GPC fine Recycled Aggregates (RA), at replacement levels of 25% and 50%, on the main mechanical properties of PC and GPC mortar mixes. The results were compared with those obtained by corresponding mixes incorporating natural and PC-RA. The main physical properties of GPC-RA were examined and proven to be comparable to those of PC-RA and slightly inferior to those of natural sand. A negligible effect was observed at 28-day compressive and flexural strength of PC mortars with GPC aggregates having a milder effect than PC. As far as GPC mortars are concerned, the influence of GPC aggregates was enhancing for the investigated mechanical properties. Additionally, a screening test showed that recycled geopolymer aggregates are not prone of inducing alkali silica reaction.
Abstract: Cancer affects people globally with breast cancer being a leading killer. Breast cancer is due to the uncontrollable multiplication of cells resulting in a tumour or neoplasm. Tumours are called ‘benign’ when cancerous cells do not ravage other body tissues and ‘malignant’ if they do so. As mammography is an effective breast cancer detection tool at an early stage which is the most treatable stage it is the primary imaging modality for screening and diagnosis of this cancer type. This paper presents an automatic mammogram classification technique using wavelet and Gabor filter. Correlation feature selection is used to reduce the feature set and selected features are classified using different decision trees.
Abstract: The aim of this audit was to examine the efficiency of alcohol history documentation and screening for hazardous drinkers at the Medical Admission Unit (MAU) of Northern General Hospital (NGH), Sheffield, to identify any potential for enhancing clinical practice. Data were collected from medical clerking sheets, ICE system and directly from 82 patients by three junior medical doctors using both CAGE questionnaire and AUDIT-C tool for newly admitted patients to MAU in NGH, in the period between January and March 2015. Alcohol consumption was documented in around two-third of the patient sample and this was documented fairly accurately by health care professionals. Some used subjective words such as 'social drinking' in the alcohol units’ section of the history. CAGE questionnaire was applied to only four patients and none of the patients had documented advice, education or referral to an alcohol liaison team. AUDIT-C tool had identified 30.4%, while CAGE 10.9%, of patients admitted to the NGH MAU as hazardous drinkers. The amount of alcohol the patient consumes positively correlated with the score of AUDIT-C (Pearson correlation 0.83). Re-audit is planned to be carried out after integrating AUDIT-C tool as labels in the notes and presenting a brief teaching session to junior doctors. Alcohol misuse screening is not adequately undertaken and no appropriate action is being offered to hazardous drinkers. CAGE questionnaire is poorly applied to patients and when satisfactory and adequately used has low sensitivity to detect hazardous drinkers in comparison with AUDIT-C tool. Re-audit of alcohol screening practice after introducing AUDIT-C tool in clerking sheets (as labels) is required to compare the findings and conclude the audit cycle.
Abstract: Plants as therapeutic agents are used as drug in many parts of the world. Medicinal plants are mostly used in developing countries due to culture acceptability, belief or due to lack of easy access to primary health care services. Jatropha curcas is a plant from the Euphorbiaceae family which is widely used in Northern Nigeria as an anti-diarrheal agent. This study was conducted to determine the anti-diarrheal effect of the leaf extract on castor oil induced diarrhea in albino rats. The leaves of J. curcas were collected from Balanga Local government in Gombe State, north-eastern Nigeria; due to its bioavailability. The leaves were air-dried at room temperature and ground to powder. Phytochemical screening was done and different concentrations of the extract was prepared and administered to the different categories of experimental animals. From the results, aqueous leaf extract of Jatropha curcas at doses of 200mg/Kg and 400mg/Kg was found to reduce the mean stool score as compared to control rats, however, maximum reduction was achieved with the standard drug of Loperamide (5mg/Kg). Treatment of diarrhea with 200mg/Kg of the extract did not produce any significant decrease in stool fluid content but was found to be significant in those rats that were treated with 400mg/Kg of the extract at 2hours (0.05±0.02) and 4hours (0.01±0.01). A significant reduction of diarrhea in the experimental animals signifies it to possess some anti-diarrheal activity.
Abstract: 36 strains of sulfur-oxidizing bacteria were isolated in
Southern Kazakhstan soda-saline soils and identified. Screening of
strains according bio-oxidation (destruction thiosulfate to sulfate) and
enzymatic (Thiosulfate dehydrogenises and thiosulfate reductase)
activity was conducted. There were selected modes of aeration and
culture conditions (pH, temperature), which provide optimum harvest
cells. These strains can be used in bio-melioration technology.
Abstract: This study was conducted for the investigation of
number of cellulolytic bacteria and their ability in decomposition.
Seven samples surface soil were collected on cellulose Zailiskii
Alatau slopes. Cellulolitic activity of new strains of Bacillus, isolated
from soil is determined. Isolated cellulose degrading bacteria were
screened for determination of the highest cellulose activity by
quantitative assay using Congo red, gravimetric assay and
colorimetric DNS method trough of the determination of the
parameters of sugar reduction. Strains are assigned to: B.subtilis,
B.licheniformis, B. cereus and, В. megaterium. Bacillus strains
consisting of several different types of cellulases have broad substrate
specificity of cellulase complexes formed by them. Cellulolitic
bacteria were recorded to have highest cellulase activity and selected
for optimization of cellulase enzyme production.
Abstract: Approximately 10,000 different types of dyes and
pigments are being used in various industrial applications yearly,
which include the textile and printing industries. However, these dyes
are difficult to degrade naturally once they enter the aquatic system.
Their high persistency in natural environment poses a potential health
hazard to all form of life. Hence, there is a need for alternative dye
removal strategy in the environment via bioremediation. In this study,
fungi laccase is investigated via commercial agar dyes plates and
submerged fermentation to explore the application of fungi laccase in
textile dye wastewater treatment. Two locally isolated basidiomycetes
were screened for laccase activity using media added with commercial
dyes such as 2, 2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid
(ABTS), guaiacol and Remazol Brillant Blue R (RBBR). Isolate TBB3
(1.70±0.06) and EL2 (1.78±0.08) gave the highest results for ABTS
plates with the appearance of greenish halo on around the isolates.
Submerged fermentation performed on Isolate TBB3 with the
productivity 3.9067 U/ml/day, whereas the laccase activity for Isolate
EL2 was much lower (0.2097 U/ml/day). As isolate TBB3 showed
higher laccase production, it was subjected to molecular
characterization by DNA isolation, PCR amplification and sequencing
of ITS region of nuclear ribosomal DNA. After being compared with
other sequences in National Center for Biotechnology Information
(NCBI database), isolate TBB3 is probably from species Trametes
hirsutei. Further research work can be performed on this isolate by
upscale the production of laccase in order to meet the demands of the
requirement for higher enzyme titer for the bioremediation of textile
dyes.
Abstract: The beginning of 21st century has witnessed new
advancements in the design and use of new materials for biosensing
applications, from nano to macro, protein to tissue. Traditional
analytical methods lack a complete toolset to describe the
complexities introduced by living systems, pathological relations,
discrete hierarchical materials, cross-phase interactions, and
structure-property dependencies. Materiomics – via systematic
molecular dynamics (MD) simulation – can provide structureprocess-
property relations by using a materials science approach
linking mechanisms across scales and enables oriented biosensor
design. With this approach, DNA biosensors can be utilized to detect
disease biomarkers present in individuals’ breath such as acetone for
diabetes. Our wireless sensor array based on single-stranded DNA
(ssDNA)-decorated single-walled carbon nanotubes (SWNT) has
successfully detected trace amount of various chemicals in vapor
differentiated by pattern recognition. Here, we present how MD
simulation can revolutionize the way of design and screening of DNA
aptamers for targeting biomarkers related to oral diseases and oral
health monitoring. It demonstrates great potential to be utilized to
build a library of DNDA sequences for reliable detection of several
biomarkers of one specific disease, and as well provides a new
methodology of creating, designing, and applying of biosensors.
Abstract: Gastric Cancer (GC) has high morbidity and fatality
rate in various countries. It is still one of the most frequent and
deadly diseases. Gastrokine1 (GKN1) and gastrokine2 (GKN2) genes
are highly expressed in the normal stomach epithelium and play
important roles in maintaining the integrity and homeostasis of
stomach mucosal epithelial cells. In this study, 47 paired samples that
were grouped according to the types of gastric cancer and the clinical
characteristics of the patients, including gender and average of age.
They were investigated with gene expression analysis and mutation
screening by monitoring RT-PCR, SSCP and nucleotide sequencing
techniques. Both GKN1 and GKN2 genes were observed significantly
reduced found by (Wilcoxon signed rank test; p
Abstract: Many herbal medicinal products are considered
potential anti-hypercholesterolemic agents with encouraging safety
profiles, however only a limited amount of clinical research exists to
support their efficacy. The present study was designed to compare the
antihypercholesterolemic and antioxidant activities of the crude
ethanolic extracts of Citrus reticulata fruit peel, Zingiber officinale
rhizome and Sesamum indicum seeds. Forty-five rats were used throughout the experiment which are
extended for four weeks. These were divided into nine groups, five
rats per each group as follows; group 1 was the normal control group
(rats only fed standard normal rat diet), group 2 was the
hypercholesterolemic control group (rats fed only
hypercholesterolemic diet which contained 1% cholesterol plus 10%
saturated animal fat added to the normal rat diet), groups 3 and 4
were fed hypercholesterolemic diet in addition to Citrus reticulata
ethanolic extract at doses of (250mg/kg (group 3) and 500mg/kg
(group 4)) administered daily via oral route, groups 5 and 6 were
given hypercholesterolemic diet in addition to Zingiber officinale
ethanolic extract at doses of (250mg/kg (group 5) and 500mg/kg
(group 6)) daily through oral route, groups 7 and 8 fed on
hypercholesterolemic diet in addition to Sesamum indicum ethanolic
extract at doses of (250mg/kg (group 7) and 500mg/kg (group 8))
daily orally; and group 9 rats were given hypercholesterolemic diet in
addition to atorvastatin (0.18mg/kg) daily via oral route as a standard
reference antihypercholesterolemic drug. Blood samples from all
groups were drawn from the retro-orbital venous plexus four weeks
following treatment after overnight fasting and the lipid profile (total
cholesterol (TC), high density lipoprotein-cholesterol (HDL-C), low
density lipoprotein-cholesterol (LDL-C) and triglyceride levels) were
measured and the risk ratio (TC/HDL-C) was assessed. The
antioxidant activity of the three plants extracts was determined using
DPPH free-radical antioxidant assay. Results of in vivo and in vitro
antihypercholesterolemic and antioxidant assay respectively, revealed
that the three extracts possess comparable antioxidant and
antihypercholesterolemic activities.
Abstract: Tannins are a unique category of plant phytochemicals
especially in terms of their vast potential health-benefiting properties.
Researchers have described the capacity of tannins to enhance
glucose uptake and inhibit adipogenesis, thus being potential drugs
for the treatment of non-insulin dependent diabetes mellitus. Thus,
the present research was conducted to find out tannin content of food
products. The percentage of tannin in various analyzed sources
ranged from 0.0 to 108.53%; highest in kathaa and lowest in ker and
mango bark. The percentage of tannins present in the plants,
however, varies. Numerous studies have confirmed that the naturally
occurring polyphenols are key factor for the beneficial effects of the
herbal medicines. Isolation and identification of active constituents
from plants, preparation of standardized dose & dosage regimen can
play a significant role in improving the hypoglycaemic action.
Abstract: Cole-Cole parameters of 40 post-menopausal women
are compared with their DEXA bone mineral density measurements.
Impedance characteristics of four extremities are compared; left and
right extremities are statistically same, but lower extremities are
statistically different than upper ones due to their different fat
content. The correlation of Cole-Cole impedance parameters to bone
mineral density (BMD) is observed to be higher for dominant arm.
With the post-menopausal population, ANOVA tests of the dominant
arm characteristic frequency, as a predictor for DEXA classified
osteopenic and osteoporic population around lumbar spine, is
statistically very significant. When used for total lumbar spine
osteoporosis diagnosis, the area under the Receiver Operating Curve
of the characteristic frequency is 0.830, suggesting that the Cole-Cole
plot characteristic frequency could be a useful diagnostic parameter
when integrated into standard screening methods for osteoporosis.
Moreover, the characteristic frequency can be directly measured by
monitoring frequency driven angular behavior of the dominant arm
without performing any complex calculation.
Abstract: In the present paper the design of plate heat exchangers
is formulated as an optimization problem considering two
mathematical modelling. The number of plates is the objective
function to be minimized, considering implicitly some parameters
configuration. Screening is the optimization method used to solve the
problem. Thermal and hydraulic constraints are verified, not viable
solutions are discarded and the method searches for the convergence to
the optimum, case it exists. A case study is presented to test the
applicability of the developed algorithm. Results show coherency with
the literature.
Abstract: Computer aided diagnosis systems provide vital
opinion to radiologists in the detection of early signs of breast cancer
from mammogram images. Architectural distortions, masses and
microcalcifications are the major abnormalities. In this paper, a
computer aided diagnosis system has been proposed for
distinguishing abnormal mammograms with architectural distortion
from normal mammogram. Four types of texture features GLCM
texture, GLRLM texture, fractal texture and spectral texture features
for the regions of suspicion are extracted. Support vector machine
has been used as classifier in this study. The proposed system yielded
an overall sensitivity of 96.47% and an accuracy of 96% for
mammogram images collected from digital database for screening
mammography database.
Abstract: This research presents the main ideas to implement an
intelligent system composed by communicating wireless sensors
measuring environmental data linked to drought indicators (such as
air temperature, soil moisture , etc...). On the other hand, the setting
up of a spatio temporal database communicating with a Web mapping
application for a monitoring in real time in activity 24:00 /day, 7
days/week is proposed to allow the screening of the drought
parameters time evolution and their extraction. Thus this system
helps detecting surfaces touched by the phenomenon of drought.
Spatio-temporal conceptual models seek to answer the users who
need to manage soil water content for irrigating or fertilizing or other
activities pursuing crop yield augmentation. Effectively, spatiotemporal
conceptual models enable users to obtain a diagram of
readable and easy data to apprehend. Based on socio-economic
information, it helps identifying people impacted by the phenomena
with the corresponding severity especially that this information is
accessible by farmers and stakeholders themselves. The study will be
applied in Siliana watershed Northern Tunisia.
Abstract: The development of transport systems has negative
impacts on the environment although it has beneficial effects on
society. The car policy caused many problems such as: - the
spectacular growth of fuel consumption hence the very vast increase
in urban pollution, traffic congestion in certain places and at certain
times, the increase in the number of accidents. The exhaust emissions
from cars and weather conditions are the main factors that determine
the level of pollution in urban atmosphere. These conditions lead to
the phenomenon of heat transfer and radiation occurring between the
air and the soil surface of any town. These exchanges give rise, in
urban areas, to the effects of heat islands that correspond to the
appearance of excess air temperature between the city and its
surrounding space. In this object, we perform a numerical simulation
of the plume generated by the cars exhaust gases and show that these
gases form a screening effect above the urban city which cause the
heat island in the presence of wind flow. This study allows us: 1. To
understand the different mechanisms of interactions between these
phenomena.2. To consider appropriate technical solutions to mitigate
the effects of the heat island.
Abstract: The aim of the present study is to investigate
consumers' determinants of intention toward the adoption of Smart
Grid solutions and technologies. Ajzen's Theory of Planned
Behaviour (TPB) model is applied and tested to explain the formation
of such adoption intention. An exogenous variable, taking into
account the resistance to change of individuals, was added to the
basic model. The elicitation study allowed obtaining salient modal
beliefs, which were used, with the support of literature, to design the
questionnaire. After the screening phase, data collected from the
main survey were analysed for evaluating measurement model's
reliability and validity. Consistent with the theory, the results of
structural equation analysis revealed that attitude, subjective norm,
and perceived behavioural control positively, which affected the
adoption intention. Specifically, the variable with the highest estimate
loading factor was found to be the perceived behavioural control,
and, the most important belief related to each construct was
determined (e.g., energy saving was observed to be the most
significant belief linked with attitude). Further investigation indicated
that the added exogenous variable has a negative influence on
intention; this finding confirmed partially the hypothesis, since this
influence was indirect: such relationship was mediated by attitude.
Implications and suggestions for future research are discussed.
Abstract: The goal of this paper is to present the diagnostic
contribution that the screening instrument, Mini-Mental State
Examination-2: Expanded Version (MMSE-2:EV), brings in
detecting the cognitive impairment or in monitoring the progress of
degenerative disorders. The diagnostic signification is underlined by
the interpretation of the MMSE-2:EV scores, resulted from the test
application to patients with mild and major neurocognitive disorders.
The cases were selected from current practice, in order to cover vast
and significant neurocognitive pathology: mild cognitive impairment,
Alzheimer’s disease, vascular dementia, mixed dementia, Parkinson’s
disease, conversion of the mild cognitive impairment into
Alzheimer’s disease. The MMSE-2:EV version was used: it was
applied one month after the initial assessment, three months after the
first reevaluation and then every six months, alternating the blue and
red forms. Correlated with age and educational level, the raw scores
were converted in T scores and then, with the mean and the standard
deviation, the z scores were calculated. The differences of raw scores
between the evaluations were analyzed from the point of view of
statistic signification, in order to establish the progression in time of
the disease. The results indicated that the psycho-diagnostic approach
for the evaluation of the cognitive impairment with MMSE-2:EV is
safe and the application interval is optimal. In clinical settings with a
large flux of patients, the application of the MMSE-2:EV is a safe
and fast psychodiagnostic solution. The clinicians can draw objective
decisions and for the patients: it does not take too much time and
energy, it does not bother them and it doesn’t force them to travel
frequently.