Cellolytic Activity of Bacteria of the Bacillus Genus Isolated from the Soil of Zailiskiy Alatau Slopes
This study was conducted for the investigation of
number of cellulolytic bacteria and their ability in decomposition.
Seven samples surface soil were collected on cellulose Zailiskii
Alatau slopes. Cellulolitic activity of new strains of Bacillus, isolated
from soil is determined. Isolated cellulose degrading bacteria were
screened for determination of the highest cellulose activity by
quantitative assay using Congo red, gravimetric assay and
colorimetric DNS method trough of the determination of the
parameters of sugar reduction. Strains are assigned to: B.subtilis,
B.licheniformis, B. cereus and, В. megaterium. Bacillus strains
consisting of several different types of cellulases have broad substrate
specificity of cellulase complexes formed by them. Cellulolitic
bacteria were recorded to have highest cellulase activity and selected
for optimization of cellulase enzyme production.
[1] Balamurugan A., Jayanthi R., Nepolean P., Vidhya R. Pallavi and
Premkumar R., Studies on cellulose degrading bacteria in tea garden
soils. African Journal of Plant Science, 2011, Vol. 5, №1: p. 22-27.
[2] Kaur M., Arora S., Isolation and Screening of Cellulose Degrading
Bacteria in Kitchen Waste and Detecting Their Degrading Potential.
IOSR Journal of Mechanical and Civil Engineering, 2012, Vol. 1, № 2:
p. 33-35.
[3] HatamiS., Alikhani H.A., Besharati H., Salehrastin N., Afroushen M.,
Yazdani Jahromi Z. Investigation on Aerobic Cellulolytic Bacteria in
Some North and Farming Soils. America-Eurasian Journal of
Agriculture and Environmental Science, 2008, Vol.3, № 2: p. 347-356.
[4] Lynd R., Lee J., Weimer P., Van Zyl W. H., Pretorius S. Microbial
cellulose utilization: Fundamental and Biotechnology. Microbiology and
Molecular Biology Rewiews, 2002, Vol.3 66, № 3: p. 506-577.
[5] Barman D., Saud Z.A., Habib M.R., Islam M.F., Hossain K., Yeasmin T.
Isolation of Cellulytic Bacterial Strains from Soil for Effective and
Efficient Bioconversion of Solid Waste. Life Sciences and Medicine
Research, 2011, Vol.5: p. 1-7.
[6] Rahman M.M., Begum M.F., Hhan M.S.I Isolation, identification and
cultural optimization of native bacteria isolates as a potential
bioconvertion agent. Journal of Applied Science Research, 2009, Vol.5:
p. 1652-1662.
[7] Elberson M.A., Malekzadeh F., Yazdi M.T. Cellulomonas persica sp.
nov. and Cellulomonas iranensis sp. nov., mesophilic cellulosedegrading
bacteria isolated from forest soils. International Journal of
Systematic and Evolutionary Microbiology, 2000, Vol.15: p. 993-996.
[8] Abdelnasser SSI, Ahmed IE, Isolation and identification of new
celluloses producing thermophilic bacteria from an Egyptian hot spring
and some properties of the crude enzyme. Austr. J. Basic App. Sci.,
2007, 1(4): p. 473-478
[9] Singh J, Batra N, Sobti RC, Purification and Characterization of alkaline
cellulose produced by a novel isolates, Bacillus sphaericus js 1. Ind
Microbiol. Biotechnol., 2004, 31: p. 51-56.
[10] Avdeeva L.V., Osadchaya A.I., Kharhota M.A. Cellolytic activity of
bacteria of genus Bacillus. Microbiology and biotechnology, 2011, №2:
p. 65-71.
[11] Avdeeva L.V., Osadchaya A.I., Kharhota M.A. Biosynthesis of
cellulases by probiotic strains Bacillussubtilis at joint cultivation.
Microbiology and biotechnology, 2010, №4: p. 80-89.
[12] Michael, Coughilan P., Mayer F. The cellulose decomposing bacteria
and their enzyme system. Appl. Environ Microbiol., 1992, 2: p. 609-61.
[13] Priit Valjamae., The Kinetics of cellulose enzymatic hydrolysis. Faculty
of science and technology, 2002: p. 781.
[14] Levy I, Shani Z, Shoseyov O, Modification of polysaccharides and plant
cell wall by endo-1,4-b-glucanase and cellulose-binding domains.
Biomolecular Engineering, 2002, 19: p. 17-30.
[1] Balamurugan A., Jayanthi R., Nepolean P., Vidhya R. Pallavi and
Premkumar R., Studies on cellulose degrading bacteria in tea garden
soils. African Journal of Plant Science, 2011, Vol. 5, №1: p. 22-27.
[2] Kaur M., Arora S., Isolation and Screening of Cellulose Degrading
Bacteria in Kitchen Waste and Detecting Their Degrading Potential.
IOSR Journal of Mechanical and Civil Engineering, 2012, Vol. 1, № 2:
p. 33-35.
[3] HatamiS., Alikhani H.A., Besharati H., Salehrastin N., Afroushen M.,
Yazdani Jahromi Z. Investigation on Aerobic Cellulolytic Bacteria in
Some North and Farming Soils. America-Eurasian Journal of
Agriculture and Environmental Science, 2008, Vol.3, № 2: p. 347-356.
[4] Lynd R., Lee J., Weimer P., Van Zyl W. H., Pretorius S. Microbial
cellulose utilization: Fundamental and Biotechnology. Microbiology and
Molecular Biology Rewiews, 2002, Vol.3 66, № 3: p. 506-577.
[5] Barman D., Saud Z.A., Habib M.R., Islam M.F., Hossain K., Yeasmin T.
Isolation of Cellulytic Bacterial Strains from Soil for Effective and
Efficient Bioconversion of Solid Waste. Life Sciences and Medicine
Research, 2011, Vol.5: p. 1-7.
[6] Rahman M.M., Begum M.F., Hhan M.S.I Isolation, identification and
cultural optimization of native bacteria isolates as a potential
bioconvertion agent. Journal of Applied Science Research, 2009, Vol.5:
p. 1652-1662.
[7] Elberson M.A., Malekzadeh F., Yazdi M.T. Cellulomonas persica sp.
nov. and Cellulomonas iranensis sp. nov., mesophilic cellulosedegrading
bacteria isolated from forest soils. International Journal of
Systematic and Evolutionary Microbiology, 2000, Vol.15: p. 993-996.
[8] Abdelnasser SSI, Ahmed IE, Isolation and identification of new
celluloses producing thermophilic bacteria from an Egyptian hot spring
and some properties of the crude enzyme. Austr. J. Basic App. Sci.,
2007, 1(4): p. 473-478
[9] Singh J, Batra N, Sobti RC, Purification and Characterization of alkaline
cellulose produced by a novel isolates, Bacillus sphaericus js 1. Ind
Microbiol. Biotechnol., 2004, 31: p. 51-56.
[10] Avdeeva L.V., Osadchaya A.I., Kharhota M.A. Cellolytic activity of
bacteria of genus Bacillus. Microbiology and biotechnology, 2011, №2:
p. 65-71.
[11] Avdeeva L.V., Osadchaya A.I., Kharhota M.A. Biosynthesis of
cellulases by probiotic strains Bacillussubtilis at joint cultivation.
Microbiology and biotechnology, 2010, №4: p. 80-89.
[12] Michael, Coughilan P., Mayer F. The cellulose decomposing bacteria
and their enzyme system. Appl. Environ Microbiol., 1992, 2: p. 609-61.
[13] Priit Valjamae., The Kinetics of cellulose enzymatic hydrolysis. Faculty
of science and technology, 2002: p. 781.
[14] Levy I, Shani Z, Shoseyov O, Modification of polysaccharides and plant
cell wall by endo-1,4-b-glucanase and cellulose-binding domains.
Biomolecular Engineering, 2002, 19: p. 17-30.
@article{"International Journal of Earth, Energy and Environmental Sciences:72103", author = "I. Savitskaya and A. Kistaubayeva and A. Zhubanova and I. Blavachinskaiya and D. Ibrayeva and M. Abdulzhanova and A. Otarbay and A.Isabekova", title = "Cellolytic Activity of Bacteria of the Bacillus Genus Isolated from the Soil of Zailiskiy Alatau Slopes", abstract = "This study was conducted for the investigation of
number of cellulolytic bacteria and their ability in decomposition.
Seven samples surface soil were collected on cellulose Zailiskii
Alatau slopes. Cellulolitic activity of new strains of Bacillus, isolated
from soil is determined. Isolated cellulose degrading bacteria were
screened for determination of the highest cellulose activity by
quantitative assay using Congo red, gravimetric assay and
colorimetric DNS method trough of the determination of the
parameters of sugar reduction. Strains are assigned to: B.subtilis,
B.licheniformis, B. cereus and, В. megaterium. Bacillus strains
consisting of several different types of cellulases have broad substrate
specificity of cellulase complexes formed by them. Cellulolitic
bacteria were recorded to have highest cellulase activity and selected
for optimization of cellulase enzyme production.", keywords = "Cellulose-degrading bacteria, cellulase complex,
foothills soil, screening.", volume = "8", number = "7", pages = "543-5", }
