Abstract: NFκB is a transcription factor regulating many
function of the vessel wall. In the normal condition , NFκB is
revealed diffuse cytoplasmic expressionsuggesting that the system is
inactive. The presence of activation NFκB provide a potential
pathway for the rapid transcriptional of a variety of genes encoding
cytokines, growth factors, adhesion molecules and procoagulatory
factors. It is likely to play an important role in chronic inflamatory
disease involved atherosclerosis. There are many stimuli with the
potential to active NFκB, including hyperlipidemia. We used 24 mice
which was divided in 6 groups. The HFD given by et libitum
procedure during 2, 4, and 6 months. The parameters in this study
were the amount of NFKB activation ,H2O2 as ROS and VCAM-1 as
a product of NFKB activation. H2O2 colorimetryc assay performed
directly using Anti Rat H2O2 ELISA Kit. The NFKB and VCAM-1
detection obtained from aorta mice, measured by ELISA kit and
imunohistochemistry. There was a significant difference activation of
H2O2, NFKB and VCAM-1 level at induce HFD after 2, 4 and 6
months. It suggest that HFD induce ROS formation and increase the
activation of NFKB as one of atherosclerosis marker that caused by
hyperlipidemia as classical atheroschlerosis risk factor.
Abstract: A nucleotide sequence can be expressed as a numerical sequence when each nucleotide is assigned its proton number. A resulting gene numerical sequence can be investigated for its fractal dimension in terms of evolution and chemical properties for comparative studies. We have investigated such nucleotide fluctuation in the 16S rRNA gene of archaea thermophiles. The studied archaea thermophiles were archaeoglobus fulgidus, methanothermobacter thermautotrophicus, methanocaldococcus jannaschii, pyrococcus horikoshii, and thermoplasma acidophilum. The studied five archaea-euryarchaeota thermophiles have fractal dimension values ranging from 1.93 to 1.97. Computer simulation shows that random sequences would have an average of about 2 with a standard deviation about 0.015. The fractal dimension was found to correlate (negative correlation) with the thermophile-s optimal growth temperature with R2 value of 0.90 (N =5). The inclusion of two aracheae-crenarchaeota thermophiles reduces the R2 value to 0.66 (N = 7). Further inclusion of two bacterial thermophiles reduces the R2 value to 0.50 (N =9). The fractal dimension is correlated (positive) to the sequence GC content with an R2 value of 0.89 for the five archaea-euryarchaeota thermophiles (and 0.74 for the entire set of N = 9), although computer simulation shows little correlation. The highest correlation (positive) was found to be between the fractal dimension and di-nucleotide Shannon entropy. However Shannon entropy and sequence GC content were observed to correlate with optimal growth temperature having an R2 of 0.8 (negative), and 0.88 (positive), respectively, for the entire set of 9 thermophiles; thus the correlation lacks species specificity. Together with another correlation study of bacterial radiation dosage with RecA repair gene sequence fractal dimension, it is postulated that fractal dimension analysis is a sensitive tool for studying the relationship between genotype and phenotype among closely related sequences.
Abstract: We develop new nonlinear methods of
immunofluorescence analysis for a sensitive technology of
respiratory burst reaction of DNA fluorescence due to oxidative
activity in the peripheral blood neutrophils. Histograms in flow
cytometry experiments represent a fluorescence flashes frequency as
functions of fluorescence intensity. We used the Shannon-Weaver
index for definition of neutrophils- biodiversity and Hurst index for
definition of fractal-s correlations in immunofluorescence for
different donors, as the basic quantitative criteria for medical
diagnostics of health status. We analyze frequencies of flashes,
information, Shannon entropies and their fractals in
immunofluorescence networks due to reduction of histogram range.
We found the number of simplest universal correlations for
biodiversity, information and Hurst index in diagnostics and
classification of pathologies for wide spectra of diseases. In addition
is determined the clear criterion of a common immunity and human
health status in a form of yes/no answers type. These answers based
on peculiarities of information in immunofluorescence networks and
biodiversity of neutrophils. Experimental data analysis has shown the
existence of homeostasis for information entropy in oxidative activity
of DNA in neutrophil nuclei for all donors.
Abstract: Infrared focal plane arrays (IRFPA) sensors, due to
their high sensitivity, high frame frequency and simple structure, have
become the most prominently used detectors in military applications.
However, they suffer from a common problem called the fixed pattern
noise (FPN), which severely degrades image quality and limits the
infrared imaging applications. Therefore, it is necessary to perform
non-uniformity correction (NUC) on IR image. The algorithms of
non-uniformity correction are classified into two main categories, the
calibration-based and scene-based algorithms. There exist some
shortcomings in both algorithms, hence a novel non-uniformity
correction algorithm based on non-linear fit is proposed, which
combines the advantages of the two algorithms. Experimental results
show that the proposed algorithm acquires a good effect of NUC with
a lower non-uniformity ratio.
Abstract: A spatial analysis of a large 20th century urban settlement (town/city) easily presents the celebrated central Business District (CBD). Theories of Urban Land Economics have easily justified and attempted to explain the existence of such a district activity area within the cityscape. This work examines the gradual emergence and development of the CBD in Lafia Town, Nigeria over 20 years and the attended urban problems caused by its emergence. Personal knowledge and observation of land use change are the main sources of data for the work, with unstructured interview with residents. The result are that the absence of a co-ordinate land use plan for the town, multi-nuclei nature, and regional location of surrounding towns have affected the growth pattern, hence the CBD. Traffic congestion, dispersed CBD land uses are some of the urban planning problems. The work concludes by advocating for integrating CBD uses.
Abstract: Breast cancer is the most common malignancy in the
world among women. Many therapies have been designed to treat
this disease. Mamectomy, chemotherapy and radiotherapy are still
the main therapies of breast cancer. However, the results were
unsatisfactory and still far from the ideal treatment.
PM 701is a natural product, has anticancer activity. The bioactive
fraction PMF and subfraction PMFK had been isolated from PM701.
PM 701 and its fractions were proved to have a cytotoxic properties
against different cancer cell lines. This article is directed for the
further examination of lyophilized PM701 and its active fractions on
the growth of breast cancer cells (MCF-7). PM 701, PMF or PMFK
were adding to the cultural medium, where MCF-7 is incubated.
PM 701, PMF or PMFK were able to inhibit significantly the
proliferation of MCF-7 cells, Moreover these new agents were
proved to induce apoptosis of the breast cancer cells; through its
direct effect on the nuclei.
Abstract: In the present study, the response of Nili Ravi buffalo
oocytes to recombinant human follicle stimulating hormone (rhFSH)
(Organon) on meiotic maturation in vitro was examined. Oocytes
were matured in vitro in medium containing either 0 or 0.05 IU/ ml
rhFSH and the stage of nuclear maturation recorded after 24 hours.
The percentage of oocytes in the control group undergoing germinal
vesicle breakdown (GVBD) observed after 24 hours of culture was
29 % whereas as in rhFSH group the percentage was 10 % were at
this stage (P< 0.001).Thus in the presence of rhFSH, a significantly
greater number of oocytes had progressed to the more advanced
stages of nuclear maturation. Indeed, the maturation of GV
(Germinal Vesicle) stage oocytes to the metaphase II (M II) stage
after 24 hours was significantly (P< 0.0001) increased by the
addition of rhFSH (82 % VS 47 %). The percentage of degenerated
oocytes after 24 hours of culture was 24 % in control group, whereas
in rhFSH group the percentage was 8 % after 24 hours. Degeneration
of the oocytes after 24 hours was not significantly (P = 0. 9361)
decreased.
Abstract: The capturing of gel electrophoresis image represents
the output of a DNA computing algorithm. Before this image is being
captured, DNA computing involves parallel overlap assembly (POA)
and polymerase chain reaction (PCR) that is the main of this
computing algorithm. However, the design of the DNA
oligonucleotides to represent a problem is quite complicated and is
prone to errors. In order to reduce these errors during the design stage
before the actual in-vitro experiment is carried out; a simulation
software capable of simulating the POA and PCR processes is
developed. This simulation software capability is unlimited where
problem of any size and complexity can be simulated, thus saving
cost due to possible errors during the design process. Information
regarding the DNA sequence during the computing process as well as
the computing output can be extracted at the same time using the
simulation software.
Abstract: Transcription factor p53 has a powerful tumor
suppressing function that is associated with many cancers. However,
p53 of the molecular weight was higher make the limitation across to
skin or cell membrane. Thymidine dinucleotide (pTT), an
oligonucleotide, can activate the p53 transcription factor. pTT is a
hydrophilic and negative charge oligonucleotide, which delivery in to
cell membrane need an appropriate carrier. The aim of this study was
to improve the bioavailability of the nucleotide fragment, thymidine
dinucleotide (pTT), using elasic liposome carriers to deliver the drug
into the skin. The study demonstrate that dioleoylphosphocholine
(DOPC) incorporated with sodium cholate at molar ratio 1:1 can
archived the particle size about 220 nm. This elastic liposome could
penetration through skin from stratum corneum to whole epidermis by
confocal laser scanning microscopy (CLSM). Moreover, we observed
the the slight increase in generation of p53 by western blot.
Abstract: Analysis of the elastic scattering of protons on 6,7Li
nuclei has been done in the framework of the optical model at the
beam energies up to 50 MeV. Differential cross sections for the 6,7Li +
p scattering were measured over the proton laboratory–energy range
from 400 to 1050 keV. The elastic scattering of 6,7Li+p data at
different proton incident energies have been analyzed using singlefolding
model. In each case the real potential obtained from the
folding model was supplemented by a phenomenological imaginary
potential, and during the fitting process the real potential was
normalized and the imaginary potential optimized. Normalization
factor NR is calculated in the range between 0.70 and 0.84.
Abstract: Light is one of the most important qualitative and
symbolic factors and has a special position in architecture and urban
development in regard to practical function. The main function of
light, either natural or artificial, is lighting up the environment and
the constructional forms which is called lighting. However, light is
used to redefine the urban spaces by architectural genius with regard
to three aesthetic, conceptual and symbolic factors. In architecture
and urban development, light has a function beyond lighting up the
environment, and the designers consider it as one of the basic
components. The present research aims at studying the function of
light and color in architectural view and their effects in buildings.
Abstract: Cross sections of As radionuclides in the interaction of natGe with 14-30 MeV protons have been deduced by off-line y-ray spectroscopy to find optimal reaction channels leading to radiotracers for positron emission tomography. The experimental results were compared with the previous results and those estimated by the compound nucleus reaction model.
Abstract: Gene, principal unit of inheritance, is an ordered
sequence of nucleotides. The genes of eukaryotic organisms include
alternating segments of exons and introns. The region of
Deoxyribonucleic acid (DNA) within a gene containing instructions
for coding a protein is called exon. On the other hand, non-coding
regions called introns are another part of DNA that regulates gene
expression by removing from the messenger Ribonucleic acid (RNA)
in a splicing process. This paper proposes to determine splice
junctions that are exon-intron boundaries by analyzing DNA
sequences. A splice junction can be either exon-intron (EI) or intron
exon (IE). Because of the popularity and compatibility of the
artificial neural network (ANN) in genetic fields; various ANN
models are applied in this research. Multi-layer Perceptron (MLP),
Radial Basis Function (RBF) and Generalized Regression Neural
Networks (GRNN) are used to analyze and detect the splice junctions
of gene sequences. 10-fold cross validation is used to demonstrate
the accuracy of networks. The real performances of these networks
are found by applying Receiver Operating Characteristic (ROC)
analysis.
Abstract: In Korea, the technology of a load fo nuclear power plant has been being developed.
automatic controller which is able to control temperature and axial power distribution was developed. identification algorithm and a model predictive contact former transforms the nuclear reactor status into
numerically. And the latter uses them and ge
manipulated values such as two kinds of control ro
this automatic controller, the performance of a coperation was evaluated. As a result, the automatic generated model parameters of a nuclear react to nuclear reactor average temperature and axial power the desired targets during a daily load follow.
Abstract: In this paper we propose a computational model for the representation and processing of morpho-phonological phenomena in a natural language, like Modern Greek. We aim at a unified treatment of inflection, compounding, and word-internal phonological changes, in a model that is used for both analysis and generation. After discussing certain difficulties cuase by well-known finitestate approaches, such as Koskenniemi-s two-level model [7] when applied to a computational treatment of compounding, we argue that a morphology-based model provides a more adequate account of word-internal phenomena. Contrary to the finite state approaches that cannot handle hierarchical word constituency in a satisfactory way, we propose a unification-based word grammar, as the nucleus of our strategy, which takes into consideration word representations that are based on affixation and [stem stem] or [stem word] compounds. In our formalism, feature-passing operations are formulated with the use of the unification device, and phonological rules modeling the correspondence between lexical and surface forms apply at morpheme boundaries. In the paper, examples from Modern Greek illustrate our approach. Morpheme structures, stress, and morphologically conditioned phoneme changes are analyzed and generated in a principled way.
Abstract: In a nuclear reactor Loss of Coolant accident (LOCA)
considers wide range of postulated damage or rupture of pipe in the
heat transport piping system. In the case of LOCA with/without
failure of emergency core cooling system in a Pressurised Heavy
water Reactor, the Pressure Tube (PT) temperature could rise
significantly due to fuel heat up and gross mismatch of the heat
generation and heat removal in the affected channel. The extent and
nature of deformation is important from reactor safety point of view.
Experimental set-ups have been designed and fabricated to simulate
ballooning (radial deformation) of PT for 220 MWe IPHWRs.
Experiments have been conducted by covering the CT by ceramic
fibers and then by submerging CT in water of voided PTs. In both
the experiments, it is observed that ballooning initiates at a
temperature around 665´┐¢C and complete contact between PT and
Caldaria Tube (CT) occurs at around 700´┐¢C approximately. The
strain rate is found to be 0.116% per second. The structural integrity
of PT is retained (no breach) for all the experiments. The PT heatup
is found to be arrested after the contact between PT and CT, thus
establishing moderator acting as an efficient heat sink for IPHWRs.
Abstract: This questionnaire-based study, aimed to measure and
compare the awareness of English reading strategies among EFL
learners at Bangkok University (BU) classified by their gender, field
of study, and English learning experience. Proportional stratified
random sampling was employed to formulate a sample of 380 BU
students. The data were statistically analyzed in terms of the mean
and standard deviation. t-Test analysis was used to find differences in
awareness of reading strategies between two groups (-male and
female- /-science and social-science students). In addition, one-way
analysis of variance (ANOVA) was used to compare reading strategy
awareness among BU students with different lengths of English
learning experience. The results of this study indicated that the
overall awareness of reading strategies of EFL learners at BU was at
a high level (ðÑ = 3.60) and that there was no statistically significant
difference between males and females, and among students who have
different lengths of English learning experience at the significance
level of 0.05. However, significant differences among students
coming from different fields of study were found at the same level of
significance.
Abstract: Resins are used in nuclear power plants for water
ultrapurification. Two approaches are considered in this work:
column experiments and simulations. A software called OPTIPUR
was developed, tested and used. The approach simulates the onedimensional
reactive transport in porous medium with convectivedispersive
transport between particles and diffusive transport within
the boundary layer around the particles. The transfer limitation in the
boundary layer is characterized by the mass transfer coefficient
(MTC). The influences on MTC were measured experimentally. The
variation of the inlet concentration does not influence the MTC; on
the contrary of the Darcy velocity which influences. This is consistent
with results obtained using the correlation of Dwivedi&Upadhyay.
With the MTC, knowing the number of exchange site and the relative
affinity, OPTIPUR can simulate the column outlet concentration
versus time. Then, the duration of use of resins can be predicted in
conditions of a binary exchange.
Abstract: This paper looks into detailed investigation of
thermal-hydraulic characteristics of the flow field in a fuel rod
model, especially near the spacer. The area investigate represents a
source of information on the velocity flow field, vortex, and on the
amount of heat transfer into the coolant all of which are critical for
the design and improvement of the fuel rod in nuclear power plants.
The flow field investigation uses three-dimensional Computational
Fluid Dynamics (CFD) with the Reynolds stresses turbulence model
(RSM). The fuel rod model incorporates a vertical annular channel
where three different shapes of spacers are used; each spacer shape is
addressed individually. These spacers are mutually compared in
consideration of heat transfer capabilities between the coolant and
the fuel rod model. The results are complemented with the calculated
heat transfer coefficient in the location of the spacer and along the
stainless-steel pipe.
Abstract: The mixed oxide nuclear fuel (MOX) of U and Pu contains several percent of fission products and minor actinides, such as neptunium, americium and curium. It is important to determine accurately the decay heat from Curium isotopes as they contribute significantly in the MOX fuel. This heat generation can cause samples to melt very quickly if excessive quantities of curium are present. In the present paper, we introduce a new approach that can predict the decay heat from curium isotopes. This work is a part of the project funded by King Abdulaziz City of Science and Technology (KASCT), Long-Term Comprehensive National Plan for Science, Technology and Innovations, and take place in King Abdulaziz University (KAU), Saudi Arabia. The approach is based on the numerical solution of coupled linear differential equations that describe decays and buildups of many nuclides to calculate the decay heat produced after shutdown. Results show the consistency and reliability of the approach applied.