Abstract: The main aim is to perform mutational analysis of CTLA4 gene Exon 1 in SLE patients. A total of 61 SLE patients fulfilling “American College of Rheumatology (ACR) criteria" and 61 controls were enrolled in this study. The region of CTLA4 gene exon 1 was amplified by using Step-down PCR technique. Extracted DNA of band 354 bp was sequenced to analyze mutations in the exon-1 of CTLA-4 gene. Further, protein sequences were identified from nucleotide sequences of CTLA4 Exon 1 by using Expasy software and through Blast P software it was found that CTLA4 protein sequences of Pakistani SLE patients were similar to that of Chinese SLE population. No variations were found after patients sequences were compared with that of the control sequence. Furthermore it was found that CTLA4 protein sequences of Pakistani SLE patients were similar to that of Chinese SLE population. Thus CTLA4 gene may not be responsible for an autoimmune disease SLE.
Abstract: In this paper, the effect of bolt clamping force on the fatigue behavior of bolted single lap joints of aluminum alloy 2024- T3 have been studied using numerical finite element method. To do so, a three dimensional model according to the bolted single lap joint has been created and numerical analysis has been carried out using finite element based package. Then the stress distribution and also the slip amplitudes have been calculated in the critical regions and the outcome have been compared with the available experimental fatigue tests results. The numerical results show that in low applied clamping force, the fatigue failure of the specimens occur around the stress concentration location (the bolted hole edge) due to the tensile stresses and thus fatigue crack propagation, but with increase of the clamping force, the fatigue life increases and the cracks nucleate and propagate far from the hole edge because of fretting fatigue. In other words, with the further increase of clamping force value of the joint, the fatigue life reduces due to occurrence of the fretting fatigue in the critical location where the slip amplitude is within its critical occurs earlier.
Abstract: Size based filtration is one of the common methods
employed to isolate circulating tumor cells (CTCs) from whole
blood. It is well known that this method suffers from isolation
efficiency to purity tradeoff. However, this tradeoff is poorly
understood. In this paper, we present the design and manufacturing
of a special rectangular slit filter. The filter was designed to retain
maximal amounts of nucleated cells, while minimizing the pressure
on cells, thereby preserving their morphology. The key parameter,
namely, input pressure, was optimized to retain the maximal number
of tumor cells, whilst maximizing the depletion of normal blood cells
(red and white blood cells and platelets). Our results indicate that for
a slit geometry of 5 × 40 μm on a 13 mm circular membrane with a
fill factor of 21%, a pressure of 6.9 mBar yields the optimum for
maximizing isolation of MCF-7 and depletion of normal blood cells.
Abstract: Virtual environment induces simulator sickness effect
for some users. The purpose of this research is to compare the
simulation sickness relative with parallax affect in one-screen and
three-screen HoloStageTM system, measured by Simulation Sickness
Questionnaire (SSQ). The results show the subjects tested in
three-screen has less sickness than one-screen and effect from the
Oculomotor (O) more than from the Disorientation (D) and more than
from the Nausea (N) or represented in O>D>N.
Abstract: The characterization of κ-carrageenan could provide a
better understanding of its functions in biological, medical and
industrial applications. Chemical and physical analyses of
carrageenan from seaweeds, Euchema cottonii L., were done to offer
information on its properties and the effects of Co-60 γ-irradiation on
its thermochemical characteristics. The structural and morphological
characteristics of κ-carrageenan were determined using scanning
electron microscopy (SEM) while the composition, molecular weight
and thermal properties were determined using attenuated total
reflectance Fourier transform infrared spectroscopy (ATR-FTIR), gel
permeation chromatography (GPC), thermal gravimetric analysis
(TGA) and differential scanning calorimetry (DSC). Further chemical
analysis was done using hydrogen-1 nuclear magnetic resonance (1H
NMR) and functional characteristics in terms of biocompatibility
were evaluated using cytotoxicity test.
Abstract: This is a genetic comparison study of Arabian Oryx
(Oryx leucoryx) population at two different locations (A &B) based
on nuclear microsatellite DNA markers. Arabian Oryx is listed as
vulnerable and endanger by the World Conservation Union (IUCN).
Thirty microsatellite markers from bovine family were applied to
investigate the genetic diversity of the Arabian Oryx and to set up a
molecular inventory. Among 30 microsatellite markers used, 13
markers were moderately polymorphic. Arabian Oryx at location A
has shown better gene diversity over location B. However, mean
number of alleles were less than location B. Data of within
population inbreeding coefficient indicates inbreeding at both
locations (A&B). Based on the analysis of polymorphic microsatellite
markers, the study revealed that Arabian Oryx need a genetically
designed breeding program.
Abstract: The modern world is experiencing fundamental and dynamic changes. The transformation of international relations; the end of confrontation and successive overcoming of the Cold War consequences have expanded possible international cooperation. The global nuclear conflict threat has been minimized, while a tendency to establish a unipolar world structure with the U.S. economic and power domination is growing. The current world system of international relations, apparently is secular. However, the religious beliefs of one or another nations play a certain (sometimes a key) role, both in the domestic affairs of the individual countries and in the development of bilateral ties. Political situation in Central Asia has been characterized by new factors such as international terrorism; religious extremism and radicalism; narcotrafficking and illicit arms trade of a global character immediately threaten to peace and political stability in Central Asia. The role and influence of Islamic fundamentalism is increasing; political ethnocentrism and the associated aggravation of inter-ethnic relations, the ambiguity of national interests and objectives of major geo-political groups in the Central Asian region regarding the division the political influence, emerge. This article approaches the following issues: the role of Islam in Central Asia; destabilizing factors in Central Asia; Islamic movements in Central Asia, Western Europe and the United States; the United States, Western Europe and Central Asia: religion, politics, ideology, and the US-Central Asia antiterrorism and religious extremism cooperation.
Abstract: Measurements of radioactivity in the environment is of great importance to monitor and control the levels of radiation to which man is exposed directly or indirectly. It is necessary to show that regardless of working or being close to nuclear power plants, people are daily in contact with some amount of radiation from the actual environment and food that are ingested, contradicting the view of most of them. The aim of this study was to analyze the rate of natural and artificial radiation from radionuclides present in cement, soil and fertilizers used in Sergipe State – Brazil. The radionuclide activitiesmeasured all samples arebelow the Brazilian limit of the exclusion and exemption criteria from the requirement of radiation protection.It was detected Be-7 in organic fertilizers that means a short interval between the brewing processes for use in agriculture. It was also detected an unexpected Cs-137 in some samples; however its activities does not represent risk for the population. Th-231 was also found in samples of soil and cement in the state of Sergipe that is an unprecedented result.
Abstract: We demonstrate a nonfaradaic electrochemical impedance spectroscopy measurement of biochemically modified gold plated electrodes using a two-electrode system. The absence of any redox indicator in the impedance measurements provide more precise and accurate characterization of the measured bioanalyte at molecular resolution. An equivalent electrical circuit of the electrodeelectrolyte interface was deduced from the observed impedance data of saline solution at low and high concentrations. The detection of biomolecular interactions was fundamentally correlated to electrical double-layer variation at modified interface. The investigations were done using 20mer deoxyribonucleic acid (DNA) strands without any label. Surface modification was performed by creating mixed monolayer of the thiol-modified single-stranded DNA and a spacer thiol (mercaptohexanol) by a two-step self-assembly method. The results clearly distinguish between the noncomplementary and complementary hybridization of DNA, at low frequency region below several hundreds Hertz.
Abstract: Dexamethasone (Dex) is a synthetic glucocorticoid
that is used in therapy. However prolonged treatments with high
doses are often required. This causes side effects that interfere with
the activity of several endocrine systems, including the gonadotropic
axis.
The aim of our study is to determine the effect of Dex on testicular
function in prepubertal Wistar rats.
Newborn Wistar rats are submitted to intraperitoneal injection of
Dex (1μg of Dex dissolved in NaCl 0.9% / 5g bw) for 20 days and
then sacrificed at the age of 40days. A control group received NaCl
0.9%. The rat is weighed daily. The plasmatic levels of testosterone,
LH and FSH were measured by radioimmunoassay. A histomorphometric
study was performed on sections of testis.
Treated groups showed a significant decrease in body weight (p
Abstract: ZnO nanostructures including nanowires, nanorods,
and nanoneedles were successfully deposited on GaAs substrates,
respectively, by simple two-step chemical method for the first time. A
ZnO seed layer was firstly pre-coated on the O2-plasma treated
substrate by sol-gel process, followed by the nucleation of ZnO
nanostructures through hydrothermal synthesis. Nanostructures with
different average diameter (15-250 nm), length (0.9-1.8 μm), density
(0.9-16×109 cm-2) were obtained via adjusting the growth time and
concentration of precursors. From the reflectivity spectra, we
concluded ordered and taper nanostructures were preferential for
photovoltaic applications. ZnO nanoneedles with an average diameter
of 106 nm, a moderate length of 2.4 μm, and the density of 7.2×109
cm-2 could be synthesized in the concentration of 0.04 M for 18 h.
Integrated with the nanoneedle array, the power conversion efficiency
of single junction solar cell was increased from 7.3 to 12.2%,
corresponding to a 67% improvement.
Abstract: The main objective developed in this paper is to find a
graphic technique for modeling, simulation and diagnosis of the
industrial systems. This importance is much apparent when it is about
a complex system such as the nuclear reactor with pressurized water
of several form with various several non-linearity and time scales. In
this case the analytical approach is heavy and does not give a fast
idea on the evolution of the system. The tool Bond Graph enabled us
to transform the analytical model into graphic model and the
software of simulation SYMBOLS 2000 specific to the Bond Graphs
made it possible to validate and have the results given by the
technical specifications. We introduce the analysis of the problem
involved in the faults localization and identification in the complex
industrial processes. We propose a method of fault detection applied
to the diagnosis and to determine the gravity of a detected fault. We
show the possibilities of application of the new diagnosis approaches
to the complex system control. The industrial systems became
increasingly complex with the faults diagnosis procedures in the
physical systems prove to become very complex as soon as the
systems considered are not elementary any more. Indeed, in front of
this complexity, we chose to make recourse to Fault Detection and
Isolation method (FDI) by the analysis of the problem of its control
and to conceive a reliable system of diagnosis making it possible to
apprehend the complex dynamic systems spatially distributed applied
to the standard pressurized water nuclear reactor.
Abstract: The phylogenetic analysis using the most conservative
portions of 18S rRNA gene revealed the phylogenetic relationship
among the two populations where DNA divergence showed that the
nucleotides diversity value were -0.00838 for the Tanjung Dawai,
Kedah and -0.00708 for the Cherating, Pahang populations
respectively. The net nucleotide divergence among populations (Da)
was -0.0073 indicating a low polymorphism among the populations
studied. Total number of mutations in the Tanjung Dawai, Kedah
samples was higher than Cherating, Pahang samples, which are 73 and
59 respectively while shared mutations across the populations were 8,
and reveal the evolutionary in the genome of Malaysian T. gigas. The
tree topology of both populations inferred using Neigbour-joining
method by comparing 1791 bp of partial 18S rRNA sequence revealed
that T. gigas haplotypes were clustered into seven clades, suggesting
that they are genetically diverse among populations which derived
from a common ancestor.
Abstract: Dilated cardiomyopathy (DCM) is a severe
cardiovascular disorder characterized by progressive systolic
dysfunction due to cardiac chamber dilatation and inefficient
myocardial contractility often leading to chronic heart failure.
Recently, a genome-wide association studies (GWASs) on DCM
indicate that the ZBTB17 gene rs10927875 single nucleotide
polymorphism is associated with DCM. The aim of the study was to
identify the distribution of ZBTB17 gene rs10927875 polymorphism
in 50 Slovak patients with DCM and 80 healthy control subjects
using the Custom Taqman®SNP Genotyping assays. Risk factors
detected at baseline in each group included age, sex, body mass
index, smoking status, diabetes and blood pressure. The mean age of
patients with DCM was 52.9±6.3 years; the mean age of individuals
in control group was 50.3±8.9 years. The distribution of investigated
genotypes of rs10927875 polymorphism within ZBTB17 gene in the
cohort of Slovak patients with DCM was as follows: CC (38.8%), CT
(55.1%), TT (6.1%), in controls: CC (43.8%), CT (51.2%), TT
(5.0%). The risk allele T was more common among the patients with
dilated cardiomyopathy than in normal controls (33.7% versus
30.6%). The differences in genotype or allele frequencies of ZBTB17
gene rs10927875 polymorphism were not statistically significant
(p=0.6908; p=0.6098). The results of this study suggest that ZBTB17
gene rs10927875 polymorphism may be a risk factor for
susceptibility to DCM in Slovak patients with DCM. Studies of
numerous files and additional functional investigations are needed to
fully understand the roles of genetic associations.
Abstract: Wireless Sensor Networks (WSNs) have attracted the attention of many researchers. This has resulted in their rapid integration in very different areas such as precision agriculture,environmental monitoring, object and event detection and military surveillance. Due to the current WSN characteristics this technology is specifically useful in industrial areas where security, reliability and autonomy are basic, such as nuclear power plants, chemical plants, and others. In this paper we present a system based on WSNs to monitor environmental conditions around and inside a nuclear power plant, specifically, radiation levels. Sensor nodes, equipped with radiation sensors, are deployed in fixed positions throughout the plant. In addition, plant staff are also equipped with mobile devices with higher capabilities than sensors such as for example PDAs able to monitor radiation levels and other conditions around them. The system enables communication between PDAs, which form a Mobile Ad-hoc Wireless Network (MANET), and allows workers to monitor remote conditions in the plant. It is particularly useful during stoppage periods for inspection or in the event of an accident to prevent risk situations.
Abstract: The purpose of this work is to establish the theoretical
foundations for calculating and designing the sublimationcondensation
processes in chemical apparatuses which are intended
for production of ultrafine powders of crystalline and amorphous
materials with controlled fractional composition. Theoretic analysis
of the primary processes of nucleation and growth kinetics of the
clusters according to the degree of super-saturation and the
homogeneous or heterogeneous nature of nucleation has been carried
out. The engineering design procedures of desublimation processes
have been offered and tested for modification of the Claus process.
Abstract: SeqWord Gene Island Sniffer, a new program for
the identification of mobile genetic elements in sequences of bacterial chromosomes is presented. This program is based on the
analysis of oligonucleotide usage variations in DNA sequences. 3,518 mobile genetic elements were identified in 637 bacterial
genomes and further analyzed by sequence similarity and the
functionality of encoded proteins. The results of this study are stored in an open database http://anjie.bi.up.ac.za/geidb/geidbhome.
php). The developed computer program and the database provide the information valuable for further investigation of the
distribution of mobile genetic elements and virulence factors among bacteria. The program is available for download at www.bi.up.ac.za/SeqWord/sniffer/index.html.
Abstract: Nuclear matrix protein 22 (NMP22) is a FDA approved
biomarker for bladder cancer. The objective of this study is to develop
a simple NMP22 immumosensor (NMP22-IMS) for accurate
measurement of NMP22. The NMP22-IMS was constructed with
NMP22 antibody immobilized on screen-printed carbon electrodes.
The construction procedures and antibody immobilization are simple.
Results showed that the NMP22-IMS has an excellent (r2³0.95)
response range (20 – 100 ng/mL). In conclusion, a simple and reliable
NMP22-IMS was developed, capable of precisely determining urine
NMP22 level.
Abstract: The possibility of radionuclides-related contamination
of lands at agricultural holdings defines the necessity to apply special
protective measures in plant growing. The aim of researches is to
elucidate the influence of polymers applying on biological migration
of man-made anthropogenic radionuclides 90Sr and 137Cs in the
system water - soil – plant. The tests are being carried out under field
conditions with and without application of polymers in root-inhabited
media in more radioecological tension zone (with the radius of 7 km
from the Armenian Nuclear Power Plant). The polymers on the base
of K+, Caµ, KµCaµ ions were tested. Productivity of pepper
depending on the presence and type of polymer material, content of
artificial radionuclides in waters, soil and plant material has been
determined. The character of different polymers influence on the
artificial radionuclides migration and accumulation in the system
water-soil-plant and accumulation in the plants has been cleared up.
Abstract: The two-phase flow field and the motion of the free
surface in an oscillating channel are simulated numerically to assess
the methodology for simulating nuclear reacotr thermal hydraulics
under seismic conditions. Two numerical methods are compared: one
is to model the oscillating channel directly using the moving grid of
the Arbitrary Lagrangian-Eulerian method, and the other is to simulate
the effect of channel motion using the oscillating acceleration acting
on the fluid in the stationary channel. The two-phase flow field in the
oscillating channel is simulated using the level set method in both
cases. The calculated results using the oscillating acceleration are
found to coinside with those using the moving grid, and the theoretical
back ground and the limitation of oscillating acceleration are discussed.
It is shown that the change in the interfacial area between liquid and
gas phases under seismic conditions is important for nuclear reactor
thermal hydraulics.