The Comparation of Activation Nuclear Factor Kappa Beta (NFKB) at Rattus Novergicus Strain Wistar Induced by Various Duration High Fat Diet (HFD)
NFκB is a transcription factor regulating many
function of the vessel wall. In the normal condition , NFκB is
revealed diffuse cytoplasmic expressionsuggesting that the system is
inactive. The presence of activation NFκB provide a potential
pathway for the rapid transcriptional of a variety of genes encoding
cytokines, growth factors, adhesion molecules and procoagulatory
factors. It is likely to play an important role in chronic inflamatory
disease involved atherosclerosis. There are many stimuli with the
potential to active NFκB, including hyperlipidemia. We used 24 mice
which was divided in 6 groups. The HFD given by et libitum
procedure during 2, 4, and 6 months. The parameters in this study
were the amount of NFKB activation ,H2O2 as ROS and VCAM-1 as
a product of NFKB activation. H2O2 colorimetryc assay performed
directly using Anti Rat H2O2 ELISA Kit. The NFKB and VCAM-1
detection obtained from aorta mice, measured by ELISA kit and
imunohistochemistry. There was a significant difference activation of
H2O2, NFKB and VCAM-1 level at induce HFD after 2, 4 and 6
months. It suggest that HFD induce ROS formation and increase the
activation of NFKB as one of atherosclerosis marker that caused by
hyperlipidemia as classical atheroschlerosis risk factor.
[1] G. O. Young, "Synthetic structure of industrial plastics (Book style with
paper title and editor)," in Plastics, 2nd ed. vol. 3, J. Peters, Ed. New
York: McGraw-Hill, 1964, pp. 15-64.
[2] S.A. Price, " Patofisiologi : Konsep Klinis Proses Penyakit", Edisi 6.
Jakarta : EGC, 2006.
[3] L.A. Reynolds, "Cholesterol, Atherosclerosis And Coronary Disease In
The UK 1950-2000,"Volume 27. London : Wellcome Trust Centre,
2006.
[4] H. Sulastomo, "Sindroma Koroner Akut dengan gangguan Metabolik
pada Wanita Usia Muda Pengguna Kontrasepsi Hormonal",
Departement of Cardiology and Vascular Faculty of Medicine
University of Indonesia, 2010.
[5] D. Sargowo, "Atherosklerosis Its Manifestation and Danger", FK
Universitas Brawijaya, Malang, 2002.
[6] P.A. Mayes, 2003 "Lipid Yang Memiliki Makna Fisiologis" Jakarta :
EGC, 2003.
[7] G. Bonizzi , "The Two NF╬║B activation pathway and their role in innate
and adaptive immunity", Trends Immunol, 2004, 25 : 280-288.
[8] C. Monaco, " T cell-mediated signalling in immune, inflammatory and
angiogenic processes: the cascade of events leading to inflammatory
diseases. Curr Drug Targets" Inflamm Allergy; 2003, 84:695.
[9] S.X. Chen, "Active constituents against HIV-1 protease from Garcinia
mangostana" Planta Med, 2005, 62(4):381-2.
[10] E. Andreakos. 2003" Heterogeneous requirement of IkB kinase 2 for
inflammatory cytokine and matrix metalloproteinase production in
rheumatoid arthritis: implications for therapy."Arthritis Rheum,
2005,48:1901-12.
[11] Goldstein, "Molecular Medicine, The Cholesterol Quartet", 2001,
Science.
[12] S.H. Wilson,"Activated nuclear factorkappaB is present in the coronary
vasculature in experimental hypercholesterolemia. Atherosclerosis"
2000, 148:23- 30.
[13] Ridker,"Inflamation and atherosclerosis", Circulation , 2002, 115 :
1135-1143.
[1] G. O. Young, "Synthetic structure of industrial plastics (Book style with
paper title and editor)," in Plastics, 2nd ed. vol. 3, J. Peters, Ed. New
York: McGraw-Hill, 1964, pp. 15-64.
[2] S.A. Price, " Patofisiologi : Konsep Klinis Proses Penyakit", Edisi 6.
Jakarta : EGC, 2006.
[3] L.A. Reynolds, "Cholesterol, Atherosclerosis And Coronary Disease In
The UK 1950-2000,"Volume 27. London : Wellcome Trust Centre,
2006.
[4] H. Sulastomo, "Sindroma Koroner Akut dengan gangguan Metabolik
pada Wanita Usia Muda Pengguna Kontrasepsi Hormonal",
Departement of Cardiology and Vascular Faculty of Medicine
University of Indonesia, 2010.
[5] D. Sargowo, "Atherosklerosis Its Manifestation and Danger", FK
Universitas Brawijaya, Malang, 2002.
[6] P.A. Mayes, 2003 "Lipid Yang Memiliki Makna Fisiologis" Jakarta :
EGC, 2003.
[7] G. Bonizzi , "The Two NF╬║B activation pathway and their role in innate
and adaptive immunity", Trends Immunol, 2004, 25 : 280-288.
[8] C. Monaco, " T cell-mediated signalling in immune, inflammatory and
angiogenic processes: the cascade of events leading to inflammatory
diseases. Curr Drug Targets" Inflamm Allergy; 2003, 84:695.
[9] S.X. Chen, "Active constituents against HIV-1 protease from Garcinia
mangostana" Planta Med, 2005, 62(4):381-2.
[10] E. Andreakos. 2003" Heterogeneous requirement of IkB kinase 2 for
inflammatory cytokine and matrix metalloproteinase production in
rheumatoid arthritis: implications for therapy."Arthritis Rheum,
2005,48:1901-12.
[11] Goldstein, "Molecular Medicine, The Cholesterol Quartet", 2001,
Science.
[12] S.H. Wilson,"Activated nuclear factorkappaB is present in the coronary
vasculature in experimental hypercholesterolemia. Atherosclerosis"
2000, 148:23- 30.
[13] Ridker,"Inflamation and atherosclerosis", Circulation , 2002, 115 :
1135-1143.
@article{"International Journal of Medical, Medicine and Health Sciences:57545", author = "Titin Andri Wihastuti and Djanggan Sargowo", title = "The Comparation of Activation Nuclear Factor Kappa Beta (NFKB) at Rattus Novergicus Strain Wistar Induced by Various Duration High Fat Diet (HFD)", abstract = "NFκB is a transcription factor regulating many
function of the vessel wall. In the normal condition , NFκB is
revealed diffuse cytoplasmic expressionsuggesting that the system is
inactive. The presence of activation NFκB provide a potential
pathway for the rapid transcriptional of a variety of genes encoding
cytokines, growth factors, adhesion molecules and procoagulatory
factors. It is likely to play an important role in chronic inflamatory
disease involved atherosclerosis. There are many stimuli with the
potential to active NFκB, including hyperlipidemia. We used 24 mice
which was divided in 6 groups. The HFD given by et libitum
procedure during 2, 4, and 6 months. The parameters in this study
were the amount of NFKB activation ,H2O2 as ROS and VCAM-1 as
a product of NFKB activation. H2O2 colorimetryc assay performed
directly using Anti Rat H2O2 ELISA Kit. The NFKB and VCAM-1
detection obtained from aorta mice, measured by ELISA kit and
imunohistochemistry. There was a significant difference activation of
H2O2, NFKB and VCAM-1 level at induce HFD after 2, 4 and 6
months. It suggest that HFD induce ROS formation and increase the
activation of NFKB as one of atherosclerosis marker that caused by
hyperlipidemia as classical atheroschlerosis risk factor.", keywords = "High Fat Diet, NFKB, H2O2, atherosclerosis", volume = "6", number = "9", pages = "438-3", }