Effect of Recombinant Human Follicle Stimulating Hormone on Meiotic Competence of In Vitro Grown Nili Ravi Buffalo Oocytes
In the present study, the response of Nili Ravi buffalo
oocytes to recombinant human follicle stimulating hormone (rhFSH)
(Organon) on meiotic maturation in vitro was examined. Oocytes
were matured in vitro in medium containing either 0 or 0.05 IU/ ml
rhFSH and the stage of nuclear maturation recorded after 24 hours.
The percentage of oocytes in the control group undergoing germinal
vesicle breakdown (GVBD) observed after 24 hours of culture was
29 % whereas as in rhFSH group the percentage was 10 % were at
this stage (P< 0.001).Thus in the presence of rhFSH, a significantly
greater number of oocytes had progressed to the more advanced
stages of nuclear maturation. Indeed, the maturation of GV
(Germinal Vesicle) stage oocytes to the metaphase II (M II) stage
after 24 hours was significantly (P< 0.0001) increased by the
addition of rhFSH (82 % VS 47 %). The percentage of degenerated
oocytes after 24 hours of culture was 24 % in control group, whereas
in rhFSH group the percentage was 8 % after 24 hours. Degeneration
of the oocytes after 24 hours was not significantly (P = 0. 9361)
decreased.
[1] D.C. Michle, A.N., Caveney, L.C., Smith, A.J. and Watson,
"Responsiveness of bovine cumulus-oocytes complexes to porcine and
recombinant human FSH, and the effect of COc quality on
gonadotropins," 1: (14)-2003.
[2] A.B. Galway, P.S. Lapolt, A. Tsafriri, et al., "Recombinant follicle
stimulating hormone induces ovulation and tissue plasminogen activator
expression in hypophysectomized rats," Endocrionology; 127, (3023-
3028) - 1990.
[3] J. Tornell, C. Bergh and U. Selleskog, 1995. Effect of recombinant
human Gonadotrophins on oocyte meiosis and steroidogenesis in
isolated pre-ovulatory rat follicles," Hum. Reprod. 10, (1619-1622)-
1995.
[4] A.G. Byskov, C. Yding Anderson, A. Hossaini, et al, "Cumulus cells of
oocyte cumulus complexes secrete a meiosis-activating substance when
stimulated with FSH," Mol. Repod. Dev; 46, (296-305)-1997.
[5] D Rizos, F. Ward, P. Duffy, M.P. Boland, and P. Lonergan,
"Consequences of bovine oocyte maturation, fertilization or early
embryo development in vitro versus in vivo: Implications for blastocyst
yield and blastocyst yield and blastobyst quality," Mol. Reprod. Dev. 61,
(234-248)-2002.
[6] P. Sutovsky, J.E. Flechon, and A. Pavlok, "Microfilaments,
Microtubules and intermediate filaments fulfill different roles in
gonadotropin induced expansion of bovine cumulus oophorus," Reprod.
Nutr. Dev. 34, (415-425) 1994.
[7] G. Pincus, and E.V. Enzmann, "The comparative behaviour of
mammalian egg in-vivo and in-vitro," I. The activation of ovarian eggs.
J. Exp. Med. 62, (665-675)-1935.
[8] A.C. Schroeder, and J.J. Eppig, "The development capacity of mouse
oocytes that matured spontaneously in-vitro is normal," Dev. Biol. 102,
493-497 - 1984.
[9] K. Saeki, M. Hoshi, M.L. Rutledge, "In-vitro fertilization and
development of bovine oocytes matured with commercially available
follicle stimulating hormones," Theriogenology. 34, (1035-1039)-1990.
[10] M. Fukushima, and Y Fukui, "Effect of gonadotrophins and steroids on
the subsequent fertilizability of extra follicular bovine oocyte culture in
vitro," Anim. Reprod. Sci. 9, (323-332)-1985.
[11] H.H.Christ, K. Joachim, L. Monika, G. Christina, O. Jan, M.B.
Henning, E. Sabine and E.R. Ursula, . "Nuclear and cytoplasmic
maturation of mouse oocytes after treatment with synthetic meiosisactivating
sterol in vitro," Biol. Reprod. 61, (1362-1372)-1999.
[12] J.J. Eppig, J.J., O, Brien , and K. Wigglesworth , "Mammalian oocyte
growth and development in vitro," Mol. Repord . Dev. 44, (260-273)-
1996.
[13] S.K. Roy, and B.J. Treacy, "Isolation and Long-term culture of human
preantral follicles," Fertil. Steril. 59 (suppl4), (783 -790)-1993.
[14] C. Sandra, B. Barbara, C. Mimi, And M.M Maruo, "In vitro
development of sheep preantral follicles," Biol. Reprod. 60, (594)-1999.
[15] A.I. Younis, B.G Brackett, and R.A. Hosken, "Influence of serum and
hormones on bovine oocytes maturation and fertilization in -vitro,"
Gamete Res. 23, (189-201)-1989.
[16] K.A. Zuelke, A.I. Younis, .and B.G. Brackett, "Enhanced oocytes
maturation of bovine oocytes with and with protein supplementation.
Serono Symposium on Fertilization in Mammals," Boston, MA. p.47,
abs 1989.
[17] C. Anderiesz, A. Ferraretti, C. Magli, A. Fiorentino, D. Fortini, L.
Gianaroli, G.M. Jones, and A.O.Trounson, "Effect of recombinant
human Gonadotrophins on human, bovine and murine oocyte meiosis,
fertilization and embryonic development," Hum. Reprod. (15:1148)-
2000.
[18] J.L. Tremoleda, T. Tharasanit, H.A.T.Van Tol, S.T.E. Stout, B.
Colenbrander, and M.N. Bevers, "Effects of follicular cells and FSH on
the resumption of meiosis in equine oocytes matured in vitro," Biol.
Reprod. 125, (665-577)-2003.
[19] D.T. Armstrong, X. Zhang, and B.C. Vanderhyder, "Hormonal actions
during oocyte maturation influence, fertilization and early embryonic
development," Ann. N. Y Acad.Sci. 626, (137-158)-1991.
[20] R.G. Cortvrind, Y. Hu, Gunliu, and E.J. Gohan, "Timed analysis of the
nuclear maturation of oocytes in early preantral mouse follicle culture
supplemented with recombinant gonadotrophins," Fertil. Steril. 70,
(1114-1125) -1998.
[1] D.C. Michle, A.N., Caveney, L.C., Smith, A.J. and Watson,
"Responsiveness of bovine cumulus-oocytes complexes to porcine and
recombinant human FSH, and the effect of COc quality on
gonadotropins," 1: (14)-2003.
[2] A.B. Galway, P.S. Lapolt, A. Tsafriri, et al., "Recombinant follicle
stimulating hormone induces ovulation and tissue plasminogen activator
expression in hypophysectomized rats," Endocrionology; 127, (3023-
3028) - 1990.
[3] J. Tornell, C. Bergh and U. Selleskog, 1995. Effect of recombinant
human Gonadotrophins on oocyte meiosis and steroidogenesis in
isolated pre-ovulatory rat follicles," Hum. Reprod. 10, (1619-1622)-
1995.
[4] A.G. Byskov, C. Yding Anderson, A. Hossaini, et al, "Cumulus cells of
oocyte cumulus complexes secrete a meiosis-activating substance when
stimulated with FSH," Mol. Repod. Dev; 46, (296-305)-1997.
[5] D Rizos, F. Ward, P. Duffy, M.P. Boland, and P. Lonergan,
"Consequences of bovine oocyte maturation, fertilization or early
embryo development in vitro versus in vivo: Implications for blastocyst
yield and blastocyst yield and blastobyst quality," Mol. Reprod. Dev. 61,
(234-248)-2002.
[6] P. Sutovsky, J.E. Flechon, and A. Pavlok, "Microfilaments,
Microtubules and intermediate filaments fulfill different roles in
gonadotropin induced expansion of bovine cumulus oophorus," Reprod.
Nutr. Dev. 34, (415-425) 1994.
[7] G. Pincus, and E.V. Enzmann, "The comparative behaviour of
mammalian egg in-vivo and in-vitro," I. The activation of ovarian eggs.
J. Exp. Med. 62, (665-675)-1935.
[8] A.C. Schroeder, and J.J. Eppig, "The development capacity of mouse
oocytes that matured spontaneously in-vitro is normal," Dev. Biol. 102,
493-497 - 1984.
[9] K. Saeki, M. Hoshi, M.L. Rutledge, "In-vitro fertilization and
development of bovine oocytes matured with commercially available
follicle stimulating hormones," Theriogenology. 34, (1035-1039)-1990.
[10] M. Fukushima, and Y Fukui, "Effect of gonadotrophins and steroids on
the subsequent fertilizability of extra follicular bovine oocyte culture in
vitro," Anim. Reprod. Sci. 9, (323-332)-1985.
[11] H.H.Christ, K. Joachim, L. Monika, G. Christina, O. Jan, M.B.
Henning, E. Sabine and E.R. Ursula, . "Nuclear and cytoplasmic
maturation of mouse oocytes after treatment with synthetic meiosisactivating
sterol in vitro," Biol. Reprod. 61, (1362-1372)-1999.
[12] J.J. Eppig, J.J., O, Brien , and K. Wigglesworth , "Mammalian oocyte
growth and development in vitro," Mol. Repord . Dev. 44, (260-273)-
1996.
[13] S.K. Roy, and B.J. Treacy, "Isolation and Long-term culture of human
preantral follicles," Fertil. Steril. 59 (suppl4), (783 -790)-1993.
[14] C. Sandra, B. Barbara, C. Mimi, And M.M Maruo, "In vitro
development of sheep preantral follicles," Biol. Reprod. 60, (594)-1999.
[15] A.I. Younis, B.G Brackett, and R.A. Hosken, "Influence of serum and
hormones on bovine oocytes maturation and fertilization in -vitro,"
Gamete Res. 23, (189-201)-1989.
[16] K.A. Zuelke, A.I. Younis, .and B.G. Brackett, "Enhanced oocytes
maturation of bovine oocytes with and with protein supplementation.
Serono Symposium on Fertilization in Mammals," Boston, MA. p.47,
abs 1989.
[17] C. Anderiesz, A. Ferraretti, C. Magli, A. Fiorentino, D. Fortini, L.
Gianaroli, G.M. Jones, and A.O.Trounson, "Effect of recombinant
human Gonadotrophins on human, bovine and murine oocyte meiosis,
fertilization and embryonic development," Hum. Reprod. (15:1148)-
2000.
[18] J.L. Tremoleda, T. Tharasanit, H.A.T.Van Tol, S.T.E. Stout, B.
Colenbrander, and M.N. Bevers, "Effects of follicular cells and FSH on
the resumption of meiosis in equine oocytes matured in vitro," Biol.
Reprod. 125, (665-577)-2003.
[19] D.T. Armstrong, X. Zhang, and B.C. Vanderhyder, "Hormonal actions
during oocyte maturation influence, fertilization and early embryonic
development," Ann. N. Y Acad.Sci. 626, (137-158)-1991.
[20] R.G. Cortvrind, Y. Hu, Gunliu, and E.J. Gohan, "Timed analysis of the
nuclear maturation of oocytes in early preantral mouse follicle culture
supplemented with recombinant gonadotrophins," Fertil. Steril. 70,
(1114-1125) -1998.
@article{"International Journal of Biological, Life and Agricultural Sciences:56858", author = "Muhammad Ijaz Khan and Samina Jalali and Beenish Shahid and S. A. Shami and Muhammad Ikramullah", title = "Effect of Recombinant Human Follicle Stimulating Hormone on Meiotic Competence of In Vitro Grown Nili Ravi Buffalo Oocytes", abstract = "In the present study, the response of Nili Ravi buffalo
oocytes to recombinant human follicle stimulating hormone (rhFSH)
(Organon) on meiotic maturation in vitro was examined. Oocytes
were matured in vitro in medium containing either 0 or 0.05 IU/ ml
rhFSH and the stage of nuclear maturation recorded after 24 hours.
The percentage of oocytes in the control group undergoing germinal
vesicle breakdown (GVBD) observed after 24 hours of culture was
29 % whereas as in rhFSH group the percentage was 10 % were at
this stage (P< 0.001).Thus in the presence of rhFSH, a significantly
greater number of oocytes had progressed to the more advanced
stages of nuclear maturation. Indeed, the maturation of GV
(Germinal Vesicle) stage oocytes to the metaphase II (M II) stage
after 24 hours was significantly (P< 0.0001) increased by the
addition of rhFSH (82 % VS 47 %). The percentage of degenerated
oocytes after 24 hours of culture was 24 % in control group, whereas
in rhFSH group the percentage was 8 % after 24 hours. Degeneration
of the oocytes after 24 hours was not significantly (P = 0. 9361)
decreased.", keywords = "Buffalo, in vitro, oocytes, recombinant FSH.", volume = "3", number = "9", pages = "462-4", }
