Abstract: In the present study, the response of Nili Ravi buffalo
oocytes to recombinant human follicle stimulating hormone (rhFSH)
(Organon) on meiotic maturation in vitro was examined. Oocytes
were matured in vitro in medium containing either 0 or 0.05 IU/ ml
rhFSH and the stage of nuclear maturation recorded after 24 hours.
The percentage of oocytes in the control group undergoing germinal
vesicle breakdown (GVBD) observed after 24 hours of culture was
29 % whereas as in rhFSH group the percentage was 10 % were at
this stage (P< 0.001).Thus in the presence of rhFSH, a significantly
greater number of oocytes had progressed to the more advanced
stages of nuclear maturation. Indeed, the maturation of GV
(Germinal Vesicle) stage oocytes to the metaphase II (M II) stage
after 24 hours was significantly (P< 0.0001) increased by the
addition of rhFSH (82 % VS 47 %). The percentage of degenerated
oocytes after 24 hours of culture was 24 % in control group, whereas
in rhFSH group the percentage was 8 % after 24 hours. Degeneration
of the oocytes after 24 hours was not significantly (P = 0. 9361)
decreased.