Shannon-Weaver Biodiversity of Neutrophils in Fractal Networks of Immunofluorescence for Medical Diagnostics
We develop new nonlinear methods of
immunofluorescence analysis for a sensitive technology of
respiratory burst reaction of DNA fluorescence due to oxidative
activity in the peripheral blood neutrophils. Histograms in flow
cytometry experiments represent a fluorescence flashes frequency as
functions of fluorescence intensity. We used the Shannon-Weaver
index for definition of neutrophils- biodiversity and Hurst index for
definition of fractal-s correlations in immunofluorescence for
different donors, as the basic quantitative criteria for medical
diagnostics of health status. We analyze frequencies of flashes,
information, Shannon entropies and their fractals in
immunofluorescence networks due to reduction of histogram range.
We found the number of simplest universal correlations for
biodiversity, information and Hurst index in diagnostics and
classification of pathologies for wide spectra of diseases. In addition
is determined the clear criterion of a common immunity and human
health status in a form of yes/no answers type. These answers based
on peculiarities of information in immunofluorescence networks and
biodiversity of neutrophils. Experimental data analysis has shown the
existence of homeostasis for information entropy in oxidative activity
of DNA in neutrophil nuclei for all donors.
[1] J.P. Robinson, W. O Carter, P. K. Narayanan, "Oxidative product
formation analysis by flow cytometry." In: Methods in Cell Biology, Vol
41, eds. Z. Darzynkiewicz, J.P. Robinson, H.A. Crissman (Academic
press ,San Diego 1994), pp.437-447.
[2] M.V.Filatov, E. Y. Varfolomeeva, E.A. Ivanov, "Flow cytofluorometric
detection of inflammatory processes by measuring respiratory burst
reaction of peripheral blood neutrophils," Biochemical and molecular
medicine vol.55, pp.116-121, 1995
[3] C.F. Bassoe, Li Nianyu, K Ragheb, G. Lawler, J. Sturgis, J.P. Robinson,
"Investigations of Phagosomes, Mitochondria, and Asidis Granules In
Human Neutrophils Using Fluorescence Probes," Cytometry Part B
(Clinical Cytometry),vol. 51B, pp. 21-29, 2003
[4] H.M..Shapiro. Practical Flow Cytometry John Wiley &Sons, New
York, 2003
[5] W. Weaver, C.E. Shannon, The Mathematical Theory of Communication
University of Illinois, Urbana, 1949
[6] I.I. Eliazar1, I. M. Sokolov, "Diversity of Poissonian populations."
Phys.Rev.E, vol.81, P.011122, 2010
[7] J. Feder, Fractals Plenum Press NewYork, 1988
[8] N.E.Galich, "Cytometric Distributions and Wavelet Spectra of
Immunofluorescence Noise in Medical Diagnostics ," eds . O. Dossel
and W.Schlegel (Eds.): WC 2009, IFMBE Proceedings 25/IV, Springer
Berlin Heidelberg , pp. 1936-1939,2009
[9] I.D .Vladescu, M.J. McCaule, I. Rouzina , M.C. Williams, "Mapping the
phase diagram of single DNA molecules forced-induced melting in the
presence of ethidium," Phys. Rev. Lett., Vol. 95, P. 158102, 2005
[10] N.E.Galich, M.V.Filatov, "Laser Fluorescence Fluctuation Excesses in
Molecular Immunology Experiments," Proc. SPIE, Vol. 6597, P.
6597OL, 2007
[11] N.E.Galich, M.V. Filatov, "Delay, change and bifurcation of the
immunofluorescence distribution attractors in health statuses diagnostics
and in medical treatment.," Proc. SPIE Vol. 7377, 73770C Jun. 16, 2009
[12] N.E.Galich, " Bifurcations of averaged immunofluorescence
distributions due to oxidative activity of DNA in medical diagnostics,"
(Accepted for publication), Biophys.rev.lett., to be published ,2010
[13] Haiyuan Yu¤, Yu Xia¤, V. Trifonov and M. Gerstein, ".Design
principles of molecular networks revealed by global comparisons and
composite motifs," Genome Biology, vol.7:R55 m(doi:10.1186/gb-2006-
7-7-r55),2006
[14] R. T. Glinton, P. Scerri, K. Sycara, " Towards the Understanding of
Information Dynamics in Large Scale Networked Systems,"Information
fusion.2009.fusion-09.12-th International conference.6-9 July ,Seattle,
WA pp. 794 - 801, 2009
[15] Benoit B. Mandelbrot , "Multifractal Power Law Distributions: Negative
and Critical Dimensions and Other ÔÇÿÔÇÿAnomalies,--Explained by a Simple
Example," Journal of Statistical Physics, Vol.110, Nos. 3-6,
March,pp.739-774, 2003
[1] J.P. Robinson, W. O Carter, P. K. Narayanan, "Oxidative product
formation analysis by flow cytometry." In: Methods in Cell Biology, Vol
41, eds. Z. Darzynkiewicz, J.P. Robinson, H.A. Crissman (Academic
press ,San Diego 1994), pp.437-447.
[2] M.V.Filatov, E. Y. Varfolomeeva, E.A. Ivanov, "Flow cytofluorometric
detection of inflammatory processes by measuring respiratory burst
reaction of peripheral blood neutrophils," Biochemical and molecular
medicine vol.55, pp.116-121, 1995
[3] C.F. Bassoe, Li Nianyu, K Ragheb, G. Lawler, J. Sturgis, J.P. Robinson,
"Investigations of Phagosomes, Mitochondria, and Asidis Granules In
Human Neutrophils Using Fluorescence Probes," Cytometry Part B
(Clinical Cytometry),vol. 51B, pp. 21-29, 2003
[4] H.M..Shapiro. Practical Flow Cytometry John Wiley &Sons, New
York, 2003
[5] W. Weaver, C.E. Shannon, The Mathematical Theory of Communication
University of Illinois, Urbana, 1949
[6] I.I. Eliazar1, I. M. Sokolov, "Diversity of Poissonian populations."
Phys.Rev.E, vol.81, P.011122, 2010
[7] J. Feder, Fractals Plenum Press NewYork, 1988
[8] N.E.Galich, "Cytometric Distributions and Wavelet Spectra of
Immunofluorescence Noise in Medical Diagnostics ," eds . O. Dossel
and W.Schlegel (Eds.): WC 2009, IFMBE Proceedings 25/IV, Springer
Berlin Heidelberg , pp. 1936-1939,2009
[9] I.D .Vladescu, M.J. McCaule, I. Rouzina , M.C. Williams, "Mapping the
phase diagram of single DNA molecules forced-induced melting in the
presence of ethidium," Phys. Rev. Lett., Vol. 95, P. 158102, 2005
[10] N.E.Galich, M.V.Filatov, "Laser Fluorescence Fluctuation Excesses in
Molecular Immunology Experiments," Proc. SPIE, Vol. 6597, P.
6597OL, 2007
[11] N.E.Galich, M.V. Filatov, "Delay, change and bifurcation of the
immunofluorescence distribution attractors in health statuses diagnostics
and in medical treatment.," Proc. SPIE Vol. 7377, 73770C Jun. 16, 2009
[12] N.E.Galich, " Bifurcations of averaged immunofluorescence
distributions due to oxidative activity of DNA in medical diagnostics,"
(Accepted for publication), Biophys.rev.lett., to be published ,2010
[13] Haiyuan Yu¤, Yu Xia¤, V. Trifonov and M. Gerstein, ".Design
principles of molecular networks revealed by global comparisons and
composite motifs," Genome Biology, vol.7:R55 m(doi:10.1186/gb-2006-
7-7-r55),2006
[14] R. T. Glinton, P. Scerri, K. Sycara, " Towards the Understanding of
Information Dynamics in Large Scale Networked Systems,"Information
fusion.2009.fusion-09.12-th International conference.6-9 July ,Seattle,
WA pp. 794 - 801, 2009
[15] Benoit B. Mandelbrot , "Multifractal Power Law Distributions: Negative
and Critical Dimensions and Other ÔÇÿÔÇÿAnomalies,--Explained by a Simple
Example," Journal of Statistical Physics, Vol.110, Nos. 3-6,
March,pp.739-774, 2003
@article{"International Journal of Medical, Medicine and Health Sciences:57436", author = "N.E.Galich", title = "Shannon-Weaver Biodiversity of Neutrophils in Fractal Networks of Immunofluorescence for Medical Diagnostics", abstract = "We develop new nonlinear methods of
immunofluorescence analysis for a sensitive technology of
respiratory burst reaction of DNA fluorescence due to oxidative
activity in the peripheral blood neutrophils. Histograms in flow
cytometry experiments represent a fluorescence flashes frequency as
functions of fluorescence intensity. We used the Shannon-Weaver
index for definition of neutrophils- biodiversity and Hurst index for
definition of fractal-s correlations in immunofluorescence for
different donors, as the basic quantitative criteria for medical
diagnostics of health status. We analyze frequencies of flashes,
information, Shannon entropies and their fractals in
immunofluorescence networks due to reduction of histogram range.
We found the number of simplest universal correlations for
biodiversity, information and Hurst index in diagnostics and
classification of pathologies for wide spectra of diseases. In addition
is determined the clear criterion of a common immunity and human
health status in a form of yes/no answers type. These answers based
on peculiarities of information in immunofluorescence networks and
biodiversity of neutrophils. Experimental data analysis has shown the
existence of homeostasis for information entropy in oxidative activity
of DNA in neutrophil nuclei for all donors.", keywords = "blood and cells fluorescence in diagnostics ofdiseases, cytometric histograms, entropy and information in fractalnetworks of oxidative activity of DNA, long-range chromosomalcorrelations in living cells.", volume = "4", number = "9", pages = "447-12", }