Abstract: The capturing of gel electrophoresis image represents
the output of a DNA computing algorithm. Before this image is being
captured, DNA computing involves parallel overlap assembly (POA)
and polymerase chain reaction (PCR) that is the main of this
computing algorithm. However, the design of the DNA
oligonucleotides to represent a problem is quite complicated and is
prone to errors. In order to reduce these errors during the design stage
before the actual in-vitro experiment is carried out; a simulation
software capable of simulating the POA and PCR processes is
developed. This simulation software capability is unlimited where
problem of any size and complexity can be simulated, thus saving
cost due to possible errors during the design process. Information
regarding the DNA sequence during the computing process as well as
the computing output can be extracted at the same time using the
simulation software.
Abstract: Oleic acid (C18:1) play an important role in
proliferation of fat cells. In this study, the effect of oleate on cells
viability in 3T3-L1 cells (fat cells) was investigated. The 3T3-L1
cells were treated with various concentrations of oleate in the
presence of 23 mM glucose. Oleate was added to adipogenic media
(day 0) to investigate the influence of oleate on proliferation of
postconfluent preadipocytes after 24 h induction. 0.1 mM oleate
promoted cell division by increasing 33.9% number of cells from
basal control in postconfluent preadipocytes. However, there were no
significantly different in cells viability with control cells when oleate
concentrations were increased up to 0.5 mM. When added to
differentiated adipocytes (day 12) for 48 h, the number of cells
decreased as oleate concentrations increased. 92.7% of cells lost
demonstrated apoptosis and necrosis after 48 h with 0.5 mM oleate.
The fluorochrome staining was examined under fluorescence
microscopy using acridine orange and ethidium bromide double
staining. Furthermore, the presence of high lactate (60.6% increased
from basal control) released into plasma has shown the direct
cytotoxicity of 0.5 mM oleate on adipocytes.