Abstract: Anchovy (Engraulis Encrasicholus) and sardine
(Sardina Pilchardus) are blue fishes linked to our alimentary tradition
of Mediterranean. In our work, particularly, we tested for the first
time physical and enzymatic methods to verify the freshness of
species of blue fish, anchovy and sardine of Mediterranean. In
connection with to the lowering of the pH after post-mortem stage we
assisted to a increase in proteolytic activity of calpaine and catpsine.
Already after 2 h in post-mortem there was a significant increase.
Abstract: Shadoo protein (Sho) was described in 2003 as the newest member of Prion protein superfamily [1]. Sho has similar structural motifs like prion protein (PrP) that is known for its central role in transmissible spongiform enchephalopathies. Although a great number of functions have been proposed, the exact physiological function of PrP is not known yet. Investigation of the function and localization of Sho may help us to understand the function of the Prion protein superfamily. Analyzing the subcellular localization of YFP-tagged forms of Sho, we detected the protein in the plasma membrane and in the nucleus of various cell lines. To reveal the localization of the endogenous protein we generated antibodies against Shadoo as well as employed commercially available anti-Shadoo antibodies: i) EG62 anti-mouse Shadoo antibody generated by Eurogentec Ltd.; ii) S-12 anti-human Shadoo antibody by Santa Cruz Biotechnology Inc.; iii) R-12 anti-mouse Shadoo antibody by Santa Cruz Biotechnology Inc.; iv) SPRN antibody against human Shadoo by Abgent Inc. We carried out immunocytochemistry on non-transfected HeLa, Zpl 2-1, Zw 3-5, GT1-1, GT1-7 and SHSY5Y cells as well as on YFP-Sho, Sho-YFP, and YFP-GPI transfected HeLa cells. Their specificity (in antibody-peptide competition assay) and co-localization (with the YFP signal) were assessed.
Abstract: Hemorrhage Disease of Grass Carp (HDGC) is a kind
of commonly occurring illnesses in summer, and the extremely high
death rate result in colossal losses to aquaculture. As the complex
connections among each factor which influences aquiculture diseases,
there-s no quit reasonable mathematical model to solve the problem at
present.A BP neural network which with excellent nonlinear mapping
coherence was adopted to establish mathematical model;
Environmental factor, which can easily detected, such as breeding
density, water temperature, pH and light intensity was set as the main
analyzing object. 25 groups of experimental data were used for
training and test, and the accuracy of using the model to predict the
trend of HDGC was above 80%. It is demonstrated that BP neural
network for predicating diseases in HDGC has a particularly
objectivity and practicality, thus it can be spread to other aquiculture
disease.
Abstract: This experiment was carried out to study the effect of
AMF, drought stress and phosphorus on physiological growth indices of basil at Iran using by a split-plot design with three replications.
The main-plot factor included: two levels of irrigation regimes (control=no drought stress and irrigation after 80 evaporation=
drought stress condition) while the sub-plot factors included
phosphorus (0, 35 and 70 kg/ha) and application and non-application of Glomus fasciculatum. The results showed that total dry matter
(TDM), life area index (LAI), relative growth rate (RGR) and crop growth rate (CGR) were all highly significantly different among the
phosphorus, whereas drought stress had effect of practical
significance on TDM, LAI, RGR and CGR. The results also showed that the highest TDM, LAI, RGR and CGR were obtained from
application of Glomus fasciculatum under no-drought condition.
Abstract: Sugarcane Shoots is an abundantly available
residual resources consisting of lignocelluloses which take it into
the benefit. The present study was focused on utilizing of
sugarcane shoot for reducing sugar production as a substrate in
ethanol production. Physical and chemical pretreatments of
sugarcane shoot were investigated. Results showed that the size of
sugarcane shoot influenced the cellulose content. The maximum
cellulose yield (60 %) can be obtained from alkaline pretreated
sugarcane shoot with 1.0 M NaOH at 30 oC for 90 min. The
cellulose yield reached up to 93.9% (w/w). Enzymatically
hydrolyzed of cellulosic residual in 0.04 citrate buffer (pH 5) with
celluclast 1.5L (0.7 FPU/ml) resulted in the highest amount of
reducing sugar at a rate of 32.1 g/l after 4 h incubation at 50°C,
and 100 oC for 5 min . Cellulose conversion was 55.5%.
Abstract: Nowadays, biometrical characterizations of Artemia
cysts are used as one of the most important factors in the study of
Artemia populations and intraspecific particularity; meanwhile these
characters can be used as economical indices. For example, typically
high hatching efficiency is possible due to the small diameter of
cysts (high number per gram); therefore small diameter of cysts
show someway high quality of cysts. This study was performed
during a ten year period, including two different ecological
conditions: rainy and drought. It is important from two different
aspects because it covers alteration of A. urmiana during ten years
also its variation in the best and worst environmental situations in
which salinity increased from 173.8 ppt in 1994 to 280.8 ppt in
2003/4. In this study the biometrical raw data of Artemia urmiana
cysts at seven stations from the Urmia Lake in 1994 and their seven
identical locations at 26 studied stations in 2003/4 were reanalyzed
again and compared together. Biometrical comparison of untreated
and decapsulated cysts in each of the seven similar stations showed a
highly significant variation between 1994 and 2003/4. Based on this
study, in whole stations the untreated and decapsulated cysts from
1994 were larger than cysts of 2003/4 without any exception. But
there was no logical relationship between salinity and chorion
thickness in the Urmia Lake. With regard to PCA analyses the
stations of two different studied years certainly have been separated
with factor 1 from each other. In conclusion, the interaction between
genetic and environmental factors can determine and explain
variation in the range of cysts diameter in Artemia.
Abstract: National Biodiversity Database System (NBIDS) has
been developed for collecting Thai biodiversity data. The goal of this
project is to provide advanced tools for querying, analyzing,
modeling, and visualizing patterns of species distribution for
researchers and scientists. NBIDS data record two types of datasets:
biodiversity data and environmental data. Biodiversity data are
specie presence data and species status. The attributes of biodiversity
data can be further classified into two groups: universal and projectspecific
attributes. Universal attributes are attributes that are common
to all of the records, e.g. X/Y coordinates, year, and collector name.
Project-specific attributes are attributes that are unique to one or a
few projects, e.g., flowering stage. Environmental data include
atmospheric data, hydrology data, soil data, and land cover data
collecting by using GLOBE protocols. We have developed webbased
tools for data entry. Google Earth KML and ArcGIS were used
as tools for map visualization. webMathematica was used for simple
data visualization and also for advanced data analysis and
visualization, e.g., spatial interpolation, and statistical analysis.
NBIDS will be used by park rangers at Khao Nan National Park, and
researchers.
Abstract: Bean common mosaic necrosis virus (BCMNV) is a
potyvirus with a worldwide distribution. This virus causes serious
economic losses in Iran in many leguminoses. During 20008,
samples were collected from soybeans fields in Tehran Province.
Four isolates (S1, S2 and S3) were inoculated on 15 species of
Cucurbitaceae, Chenopodiaceae, Solanacae and Leguminosae.
Chenopodium quinoa and C. amaranticolor.
Did not developed any symptoms.all isolates caused mosaic
symptoms on Phaseolus vulgaris cv. Red Kidney and P. vulgaris cv.
Bountiful. The molecular weights of coat protein using SDS-PAGE
and western blotting were estimated at 33 kDa. Reverse transcription
polymerase chain reaction (RT-PCR) was performed using one
primer pairs designed by L. XU et al. An approximately 920 bp
fragment was amplified with a specific primer.
Abstract: Ten lactating multiparous Holstein cows were used in
a cross-over design with two dietary treatments and 28-d periods
(with 14 d as an adaptation) to study the effect of restaurant fat on
milk production and composition. Each cow was offered 14.7 kg
DM /d of the basal concentrate diet based on barley and corn (crude
protein = 17.7%, neutral detergent fiber = 23.5%, and acid detergent
fiber = 5.8% of dry matter) with free access to alfalfa. Dietary
treatments were arranged as supplying each cow with 0 (CONTROL)
or 150 g/day (RF) of restaurant fat. Supplemental RF did not
significantly (P > 0.25) affect milk yield, composition, and
composition yields, except for milk fat contents. Milk fat contents
were depressed (P < 0.05) with supplemental RF. Our results indicate
that RF could depress milk fat without affecting milk yield and that
the depression in milk fat in response to RF precedes the depression
in milk yield.
Abstract: The effects of coatings based on sodium alginate (S.A) and carboxyl methyl cellulose (CMC) on the color and moisture characteristics of potato round slices were investigated. It is the first time that this combination of polysaccharides is used as edible coating which alone had the best performance as inhibitor of potato color discoloration during the storage of 15 days at 4oC. When ascorbic acid (AA) and green tea (GT) were added in the above edible coating its effects on potato round slices changed. The mixtures of sodium alginate and carboxyl methyl cellulose with ascorbic acid or with green tea behave as a potential moisture barrier, resulting to the extent of potato samples self–life. These data suggests that both GT and AA are potential inhibitors of dehydration in potatoes and not only natural antioxidants.
Abstract: fibers of pure cellulose can be made from some bacteria such as acetobacter xylinum. Bacterial cellulose fibers are very pure, tens of nm across and about 0.5 micron long. The fibers are very stiff and, although nobody seems to have measured the strength of individual fibers. Their stiffness up to 70 GPa. Fundamental strengths should be at least greater than those of the best commercial polymers, but best bulk strength seems to about the same as that of steel. They can potentially be produced in industrial quantities at greatly lowered cost and water content, and with triple the yield, by a new process. This article presents a critical review of the available information on the bacterial cellulose as a biological nonwoven fabric with special emphasis on its fermentative production and applications. Characteristics of bacterial cellulose biofabric with respect to its structure and physicochemical properties are discussed. Current and potential applications of bacterial cellulose in textile, nonwoven cloth, paper, films synthetic fiber coating, food, pharmaceutical and other industries are also presented.
Abstract: The drug discovery process starts with protein
identification because proteins are responsible for many functions
required for maintenance of life. Protein identification further needs
determination of protein function. Proposed method develops a
classifier for human protein function prediction. The model uses
decision tree for classification process. The protein function is
predicted on the basis of matched sequence derived features per each
protein function. The research work includes the development of a
tool which determines sequence derived features by analyzing
different parameters. The other sequence derived features are
determined using various web based tools.
Abstract: The present experimental investigation brings about
a comparative study of lactic acid production by pure strains of
Lactobacilli (1) L. delbreuckii (NCIM2025), (2) L. pentosus (NCIM
2912), (3) Lactobacillus sp.(NCIM 2734, (4) Lactobacillus sp.
(NCIM2084) and coculture of strain-1 and Stain-2 in solid bed of
wheat bran, under the influence of different nitrogen sources such as
baker-s yeast, meat extract and proteose peptone. Among the pure
cultures, strain-3 attained lowest pH value of 3.44, hence highest acid
formation 46.41 g/L, while the coculture attained an overall
maximum value 47.56 g/L lactic acid (pH 3.38) at 15 g/L and 20 g/L
level of baker-s yeast, respectively.
Abstract: Structured phospholipids from commercial soybean
lecithin with oil enriched omega-3 fatty acid form by product of tuna
canning is alternative procedure to provides the stability of omega-3
fatty acid structure and increase these bioactive function in
metabolism. Best treatment condition was obtain in 18 hours
acidolysis reaction with 30% enzyme concentration, which EPADHA
incorporation level was 127,47 mg/g and incorporation
percentage of EPA-DHA was 51,04% at phospholipids structure.
This structured phospolipids could reduce atherosclerosis risk in
male Sprague dawley rat. Provision of structured phospholipids has
significant effect (α = 0.05) on changes in lipid profile, intima-media
thickness of aorta rats (male Sprague dawley) fed atherogenic diet.
Structured phospholipids intake can lower total cholesterol 78.36
mg/dL, total triglycerides 94,57 mg/dL, LDL levels 87.08 mg/dL and
increased HDL level as much as 12,64 mg/dL in 10 weeks cares.
Structured phospholipids intake also can prevent the thickening of
the intima-media layer of the aorta.
Abstract: Formaldehyde is the illegal chemical substance used
for food preservation in fish and vegetable. It can promote
carcinogenesis. Superoxide dismutases are the important
antioxidative enzymes that catalyze the dismutation of superoxide
anion into oxygen and hydrogen peroxide. The resultant level of
oxidative stress in formaldehyde-treated lymphocytes was
investigated. The formaldehyde concentrations of 0, 20, 40, 60, 80
and 120μmol/L were treated in human lymphocytes for 12 hours.
After 12 treated hours, the superoxide dismutase activity change was
measured in formaldehyde-treated lymphocytes. The results showed
that the formaldehyde concentrations of 60, 80 and 120μmol/L
significantly decreased superoxide dismutase activities in
lymphocytes (P < 0.05). The change of superoxide dismutase
activity in formaldehyde-treated lymphocytes may be the biomarker
for detect cellular injury, such as damage to DNA, due to
formaldehyde exposure.
Abstract: Six parameters, the effective diffusivity (De),
activation energy of De, pre-exponential factor of De, amount
(ASOW) of self-organized water species, and amplitude (α) of the
forced oscillation of the molecular mobility (1/tC) derived from the
forced cyclic temperature change operation, were characterized by
using six typical foods, squid, sardines, scallops, salmon, beef, and
pork, as a function of the correlation time (tC) of the water molecule-s
proton retained in the foods. Each of the six parameters was clearly
divided into the water species A1 and A2 at a specified value of tC
=10-8s (=CtC), indicating an anomalous change in the physicochemical
nature of the water species at the CtC. The forced oscillation of 1/tC
clearly demonstrated a characteristic mode depending on the food
shown as a three dimensional map associated with 1/tC, the amount of
self-organized water, and tC.
Abstract: To evaluate genetic variation of wheat (Triticum aestivum) affected by heat and drought stress on eight Australian wheat genotypes that are parents of Doubled Haploid (HD) mapping populations at the vegetative stage, the water stress experiment was conducted at 65% field capacity in growth room. Heat stress experiment was conducted in the research field under irrigation over summer. Result show that water stress decreased dry shoot weight and RWC but increased osmolarity and means of Fv/Fm values in all varieties except for Krichauff. Krichauff and Kukri had the maximum RWC under drought stress. Trident variety was shown maximum WUE, osmolarity (610 mM/Kg), dry mater, quantum yield and Fv/Fm 0.815 under water stress condition. However, the recovery of quantum yield was apparent between 4 to 7 days after stress in all varieties. Nevertheless, increase in water stress after that lead to strong decrease in quantum yield. There was a genetic variation for leaf pigments content among varieties under heat stress. Heat stress decreased significantly the total chlorophyll content that measured by SPAD. Krichauff had maximum value of Anthocyanin content (2.978 A/g FW), chlorophyll a+b (2.001 mg/g FW) and chlorophyll a (1.502 mg/g FW). Maximum value of chlorophyll b (0.515 mg/g FW) and Carotenoids (0.234 mg/g FW) content belonged to Kukri. The quantum yield of all varieties decreased significantly, when the weather temperature increased from 28 ÔùªC to 36 ÔùªC during the 6 days. However, the recovery of quantum yield was apparent after 8th day in all varieties. The maximum decrease and recovery in quantum yield was observed in Krichauff. Drought and heat tolerant and moderately tolerant wheat genotypes were included Trident, Krichauff, Kukri and RAC875. Molineux, Berkut and Excalibur were clustered into most sensitive and moderately sensitive genotypes. Finally, the results show that there was a significantly genetic variation among the eight varieties that were studied under heat and water stress.
Abstract: The characterization of κ-carrageenan could provide a
better understanding of its functions in biological, medical and
industrial applications. Chemical and physical analyses of
carrageenan from seaweeds, Euchema cottonii L., were done to offer
information on its properties and the effects of Co-60 γ-irradiation on
its thermochemical characteristics. The structural and morphological
characteristics of κ-carrageenan were determined using scanning
electron microscopy (SEM) while the composition, molecular weight
and thermal properties were determined using attenuated total
reflectance Fourier transform infrared spectroscopy (ATR-FTIR), gel
permeation chromatography (GPC), thermal gravimetric analysis
(TGA) and differential scanning calorimetry (DSC). Further chemical
analysis was done using hydrogen-1 nuclear magnetic resonance (1H
NMR) and functional characteristics in terms of biocompatibility
were evaluated using cytotoxicity test.
Abstract: Tomato powder has good potential as substitute of tomato paste and other tomato products. In order to protect physicochemical properties and nutritional quality of tomato during dehydration process, investigation was carried out using different drying methods and pretreatments. Solar drier and continuous conveyor (tunnel) drier were used for dehydration where as calcium chloride (CaCl2), potassium metabisulphite (KMS), calcium chloride and potassium metabisulphite (CaCl2 +KMS), and sodium chloride (NaCl) selected for treatment.. lycopene content, dehydration ratio, rehydration ratio and non-enzymatic browning in addition to moisture, sugar and titrable acidity were studied. Results show that pre-treatment with CaCl2 and NaCl increased water removal and moisture mobility in tomato slices during drying of tomatoes. Where CaCl2 used along with KMS the NEB was recorded the least compared to other treatments and the best results were obtained while using the two chemicals in combination form. Storage studies in LDPE polymeric and metalized polyesters films showed less changes in the products packed in metallized polyester pouches and even after 6 months lycopene content did not decrease more than 20% as compared to the control sample and provide extension of shelf life in acceptable condition for 6 months. In most of the quality characteristics tunnel drier samples presented better values in comparison to solar drier.
Abstract: Raisin Concentrate (RC) are the most important
products obtained in the raisin processing industries. These RC
products are now used to make the syrups, drinks and confectionery
productions and introduced as natural substitute for sugar in food
applications. Iran is a one of the biggest raisin exporter in the world
but unfortunately despite a good raw material, no serious effort to
extract the RC has been taken in Iran. Therefore, in this paper, we
determined and analyzed affected parameters on extracting RC
process and then optimizing these parameters for design the
extracting RC process in two types of raisin (round and long)
produced in Khorasan region. Two levels of solvent (1:1 and 2:1),
three levels of extraction temperature (60°C, 70°C and 80°C), and
three levels of concentration temperature (50°C, 60°C and 70°C)
were the treatments. Finally physicochemical characteristics of the
obtained concentrate such as color, viscosity, percentage of reduction
sugar, acidity and the microbial tests (mould and yeast) were
counted. The analysis was performed on the basis of factorial in the
form of completely randomized design (CRD) and Duncan's multiple
range test (DMRT) was used for the comparison of the means.
Statistical analysis of results showed that optimal conditions for
production of concentrate is round raisins when the solvent ratio was
2:1 with extraction temperature of 60°C and then concentration
temperature of 50°C. Round raisin is cheaper than the long one, and
it is more economical to concentrate production. Furthermore, round
raisin has more aromas and the less color degree with increasing the
temperature of concentration and extraction. Finally, according to
mentioned factors the concentrate of round raisin is recommended.