Some Biological and Molecular Characterization of Bean Common Mosaic Necrosis Virus Isolated from Soybean in Tehran Province, Iran
Bean common mosaic necrosis virus (BCMNV) is a
potyvirus with a worldwide distribution. This virus causes serious
economic losses in Iran in many leguminoses. During 20008,
samples were collected from soybeans fields in Tehran Province.
Four isolates (S1, S2 and S3) were inoculated on 15 species of
Cucurbitaceae, Chenopodiaceae, Solanacae and Leguminosae.
Chenopodium quinoa and C. amaranticolor.
Did not developed any symptoms.all isolates caused mosaic
symptoms on Phaseolus vulgaris cv. Red Kidney and P. vulgaris cv.
Bountiful. The molecular weights of coat protein using SDS-PAGE
and western blotting were estimated at 33 kDa. Reverse transcription
polymerase chain reaction (RT-PCR) was performed using one
primer pairs designed by L. XU et al. An approximately 920 bp
fragment was amplified with a specific primer.
[1] E. Drifhout, "Genetic Interaction Between Phaseolus vulgaris and bean
common mosaic virus with implication for strain identification and
breeding for resistance. Agriculture Reserch Report,1978, no 872.
PUDOC, Wageningen.
[2] G.E. Gálvez, and F.J. Morales." Aphid-transmitted viruses". InH.F.
Schwartz and M.A. Pastor-Corrales (eds.). Bean production problems in
the tropics. Centro Internacional de Agricultura Tropical (CIAT). Cali,
Colombia. ,1989, p. 333-362.
[3] G. J. Wadsworth, M. G. Redibaugh, and , J. G.
Scandalios," A Procedure for the Small-scale Isolation of Plant RNA
Suitable for RNA Blot Analysis", 1998, Anal. Biochem.,
172: 279-283.
[4] G. L. Mink, H. J. Vetten, C. W. Ward, P. H. Berger , F. J. Morales, J. M.
Myers, M. J. Silbernagel, O. W. Barnett, "Taxonomy and classification
of lego- infecting potyviruses: aproposal from the potyviridae study
group of the plant virus subcommittee of ICTV. Arch Virol. 1994, 139:
231-235.
[5] G. L. Mink, and M. J. Silbernagel, "serological and biological
relationship among viruses in bean common mosaic virus subgroup". In:
O. W. Barnett (ed) Potyvirus taxonomy. Springer, Wien New York,
1992, pp. 397-406.
[6] L. Bos, "Bean common mosaic virus", C. M. I./ A. A. B. Description of
Plant Viruses, No 37.
[7] N. M. Mc Kern, G. l. Mink, O. W. Barnett, A. Mishra, L. A.
WhittakerM. J. sSilbernagel, C. W. Ward, D. D. Shukla, "Isolate of bean
common mosaic virus comprising two distinc poyuvirus".
Phytopathology, 1992, 82: 923-929.
[8] W. J. Zaumeyer, H. R. Thomas, " Amonographic study of bean diseases
and methods for their control". Technical Bulletin, 1957, No. 868. U. S.
Department of Agriculture, Washington D. C.
[9] W. G. Dougherty, and B. L. Semler,"Expression of Virus Encoded
Proteinases,Functional and Structural Similarities with Cellular
Enzymes". Microbiol.,1993, Rev., 57: 781-822.
[1] E. Drifhout, "Genetic Interaction Between Phaseolus vulgaris and bean
common mosaic virus with implication for strain identification and
breeding for resistance. Agriculture Reserch Report,1978, no 872.
PUDOC, Wageningen.
[2] G.E. Gálvez, and F.J. Morales." Aphid-transmitted viruses". InH.F.
Schwartz and M.A. Pastor-Corrales (eds.). Bean production problems in
the tropics. Centro Internacional de Agricultura Tropical (CIAT). Cali,
Colombia. ,1989, p. 333-362.
[3] G. J. Wadsworth, M. G. Redibaugh, and , J. G.
Scandalios," A Procedure for the Small-scale Isolation of Plant RNA
Suitable for RNA Blot Analysis", 1998, Anal. Biochem.,
172: 279-283.
[4] G. L. Mink, H. J. Vetten, C. W. Ward, P. H. Berger , F. J. Morales, J. M.
Myers, M. J. Silbernagel, O. W. Barnett, "Taxonomy and classification
of lego- infecting potyviruses: aproposal from the potyviridae study
group of the plant virus subcommittee of ICTV. Arch Virol. 1994, 139:
231-235.
[5] G. L. Mink, and M. J. Silbernagel, "serological and biological
relationship among viruses in bean common mosaic virus subgroup". In:
O. W. Barnett (ed) Potyvirus taxonomy. Springer, Wien New York,
1992, pp. 397-406.
[6] L. Bos, "Bean common mosaic virus", C. M. I./ A. A. B. Description of
Plant Viruses, No 37.
[7] N. M. Mc Kern, G. l. Mink, O. W. Barnett, A. Mishra, L. A.
WhittakerM. J. sSilbernagel, C. W. Ward, D. D. Shukla, "Isolate of bean
common mosaic virus comprising two distinc poyuvirus".
Phytopathology, 1992, 82: 923-929.
[8] W. J. Zaumeyer, H. R. Thomas, " Amonographic study of bean diseases
and methods for their control". Technical Bulletin, 1957, No. 868. U. S.
Department of Agriculture, Washington D. C.
[9] W. G. Dougherty, and B. L. Semler,"Expression of Virus Encoded
Proteinases,Functional and Structural Similarities with Cellular
Enzymes". Microbiol.,1993, Rev., 57: 781-822.
@article{"International Journal of Biological, Life and Agricultural Sciences:59027", author = "F. S. Abtahi and M. Koohi Hbibi and M. Khodaei Motlagh", title = "Some Biological and Molecular Characterization of Bean Common Mosaic Necrosis Virus Isolated from Soybean in Tehran Province, Iran", abstract = "Bean common mosaic necrosis virus (BCMNV) is a
potyvirus with a worldwide distribution. This virus causes serious
economic losses in Iran in many leguminoses. During 20008,
samples were collected from soybeans fields in Tehran Province.
Four isolates (S1, S2 and S3) were inoculated on 15 species of
Cucurbitaceae, Chenopodiaceae, Solanacae and Leguminosae.
Chenopodium quinoa and C. amaranticolor.
Did not developed any symptoms.all isolates caused mosaic
symptoms on Phaseolus vulgaris cv. Red Kidney and P. vulgaris cv.
Bountiful. The molecular weights of coat protein using SDS-PAGE
and western blotting were estimated at 33 kDa. Reverse transcription
polymerase chain reaction (RT-PCR) was performed using one
primer pairs designed by L. XU et al. An approximately 920 bp
fragment was amplified with a specific primer.", keywords = "ELISA, RT-PCR, SDS-PAGE, BCMNV.", volume = "3", number = "1", pages = "77-4", }