Conversion of Sugarcane Shoots to Reducing Sugars

Sugarcane Shoots is an abundantly available residual resources consisting of lignocelluloses which take it into the benefit. The present study was focused on utilizing of sugarcane shoot for reducing sugar production as a substrate in ethanol production. Physical and chemical pretreatments of sugarcane shoot were investigated. Results showed that the size of sugarcane shoot influenced the cellulose content. The maximum cellulose yield (60 %) can be obtained from alkaline pretreated sugarcane shoot with 1.0 M NaOH at 30 oC for 90 min. The cellulose yield reached up to 93.9% (w/w). Enzymatically hydrolyzed of cellulosic residual in 0.04 citrate buffer (pH 5) with celluclast 1.5L (0.7 FPU/ml) resulted in the highest amount of reducing sugar at a rate of 32.1 g/l after 4 h incubation at 50°C, and 100 oC for 5 min . Cellulose conversion was 55.5%.

Authors:

• Sathida Phonoy
• Bongotrut Pitiyont
• Vichien kitpreechavanich

Keywords:

• Conversion
• Sugarcane Shoots
• Reducing Sugars.

References:

[1] Dellepiane, D., B. Bosio And E. Arato. 2003. Clean energy from
sugarcane waste: feasibility study of an innovative application of
biogases and barbojo. Powder sources. 122: 47-56.
[2] Chen, J., W. Zhang, L. Tan, Y. Wang and G. He. 2009.
Optimization of metabolic pathways for bioconversion of
lignocellulose to ethanol through genetic engineering.
Biotechnology Advances. 6 p.
[3] Parveen, K., B. M. Diane, D.J. Micheal, S. Pieter. 2009. Methods
for Pretreatment of Lignocellulosic Biomass for Efficient Hydrolysis
and Biofuel Production. Ind. Eng. Chem. Res. 48: 3713-3729.
[4] Dawson, L. and R. Boopathy. 2007. Use of post-harvest
sugarcane residue for ethanol product. Bioresource technology. 98:
1695-1699.
[5] Kuhirun, M., H. Punpayuk, S. Dumronglot and B. Kosittrup. 2003.
Report research guideline using cellulose from agricultural waste
and agricultural industry for ethanol production. Office of the
National Research Council of Thailand. 57 p.
[6] Association of Official Analytical Chemises (AOAC). 2000. Official
Method of Analysis 17th ed. Associations.
[7] TAPPI. 2000-2001. Method for determination of alphabeta-,
gramma-cellulose in pulp.(T203 om-88). Technical Association
of Pulp and paper Industry.
[8] Miller, L.G. 1959. Use of dinitrosalicylic acid for determination of
reducing sugar. Analysis Chemical. 31: 426-438.
[9] Marques, S., L. Alves, J. C. Roseiro and F. M. Giro. 2008.
Conversion of recycle paper sludge to ethanol by SHF and SSF
using Pichia stipitis. Biomass and Bioenergy. 32: 400-406.