Abstract: Sugarcane Shoots is an abundantly available
residual resources consisting of lignocelluloses which take it into
the benefit. The present study was focused on utilizing of
sugarcane shoot for reducing sugar production as a substrate in
ethanol production. Physical and chemical pretreatments of
sugarcane shoot were investigated. Results showed that the size of
sugarcane shoot influenced the cellulose content. The maximum
cellulose yield (60 %) can be obtained from alkaline pretreated
sugarcane shoot with 1.0 M NaOH at 30 oC for 90 min. The
cellulose yield reached up to 93.9% (w/w). Enzymatically
hydrolyzed of cellulosic residual in 0.04 citrate buffer (pH 5) with
celluclast 1.5L (0.7 FPU/ml) resulted in the highest amount of
reducing sugar at a rate of 32.1 g/l after 4 h incubation at 50°C,
and 100 oC for 5 min . Cellulose conversion was 55.5%.