Abstract: The effect of seed inoculation by VA- mycorrhiza and
different levels of phosphorus fertilizer on growth and yield of
sunflower (Azargol cultivar) was studied in experiment farm of
Islamic Azad University, Karaj Branch during 2008 growing season.
The experiment treatments were arranged in factorial based on a
complete randomized block design with three replications. Four
phosphorus fertilizer levels of 25%, 50% 75% and 100% P
recommended with two levels of Mycorrhiza: with and without
Mycorrhiza (control) were assigned in a factorial combination.
Results showed that head diameter, number of seeds in head, seed
yield and oil yield were significantly higher in inoculated plants than
in non-inoculated plants. Head diameter, number of seeds in head,
1000 seeds weight, biological yield, seed yield and oil yield increased
with increasing P level above 75% P recommended in non-inoculated
plants, whereas no significant difference was observed between 75%
and 100% P recommended. The positive effect of mycorrhizal
inoculation decreased with increasing P levels due to decreased
percent root colonization at higher P levels. According to the results
of this experiment, application of mycorrhiza in present of 50% P
recommended had an appropriate performance and could increase
seed yield and oil production to an acceptable level, so it could be
considered as a suitable substitute for chemical phosphorus fertilizer
in organic agricultural systems.
Abstract: Dexamethasone (Dex) is a synthetic glucocorticoid
that is used in therapy. However prolonged treatments with high
doses are often required. This causes side effects that interfere with
the activity of several endocrine systems, including the gonadotropic
axis.
The aim of our study is to determine the effect of Dex on testicular
function in prepubertal Wistar rats.
Newborn Wistar rats are submitted to intraperitoneal injection of
Dex (1μg of Dex dissolved in NaCl 0.9% / 5g bw) for 20 days and
then sacrificed at the age of 40days. A control group received NaCl
0.9%. The rat is weighed daily. The plasmatic levels of testosterone,
LH and FSH were measured by radioimmunoassay. A histomorphometric
study was performed on sections of testis.
Treated groups showed a significant decrease in body weight (p
Abstract: The aim of this study was to estimate the frequency of
EBV infection in Hodgkin's lymphoma (HL) and non-Hodgkin's
lymphoma (NHL) occurring in Jordanian patients. A total of 55
patients with lymphoma were examined in this study. Of 55 patients,
30 and 25 were diagnosed as HL and NHL, respectively. The four
HL subtypes were observed with the majority of the cases exhibited
the mixed cellularity (MC) subtype followed by the nodular sclerosis
(NS). The high grade was found to be the commonest subtype of
NHL in our sample, followed by the low grade. The presence of EBV
virus was detected by immunostating for expression of latent
membrane protein-1 (LMP-1). The frequency of LMP-1 expression
occurred more frequent in patients with HL (60.0%) than in patients
with NHL (32.0%). The frequency of LMP-1 expression was also
higher in patients with MC subtype (61.11%) than those patients with
NS (28.57%). No age or gender difference in occurrence of EBV
infection was observed among patient with HL. By contrast, the
prevalence of EBV infection in NHL patients aged below 50 was
lower (16.66%) than in NHL patients aged 50 or above (46.15%). In
addition, EBV infection was more frequent in females with NHL
(38.46%) than in male with NHL (25%). In NHL cases, the
frequency of EBV infection in intermediate grade (60.0%) was high
when compared with frequency of low (25%) or high grades (25%).
In conclusion, analysis of LMP-1 expression indicates an important
role for this viral oncogene in the pathogenesis of EBV-associated
malignant lymphomas. These data also support the previous findings
that people with EBV may develop lymphoma and that efforts to
maintain low lymphoma should be considered for people with EBV
infection.
Abstract: Heavy metal pollution is an environmental concern.
Phytoremediation is a low-cost, environmental-friendly approach to
solve this problem. Mustard has the potential in reducing heavy metal
contents in soils. Among mustard (Brassica juncea (L.) Czern &
Coss) genotypes in Sri Lanka, accessions 7788, 8831 and 5088 give
significantly a high yield. Therefore, present study was conducted to
quantify the phytoextractive potential among these local mustard
accessions and to assess the interaction of heavy metals, Pb, Co, Mn
on phytoextraction. A pot experiment was designed with acid washed
sand (quartz) and a series of heavy metal solutions of 0, 25, 50, 75
and 100 μg/g. Experiment was carried out with factorial
experimental design. Mustard accessions were tolerant to heavy
metals and could be successfully used in removal of Pb, Co and Mn
and they are capable of accumulating significant quantities of heavy
metals in vegetative and reproductive organs. The order of the
accumulative potential of Pb, Co and Mn in mustard accessions is,
root > shoot >seed.
Abstract: This experiment was conducted to investigate the
effect of dietary supplementation of different levels of black seed
(Nigella sativa L.) on the performance and immune response of broiler chicks. A total 240 day-old broiler chicks were used and
randomly allotted equally into six experimental groups designated as 1, 2, 3, 4, 5 and 6 having black seed at the rate of 0, 2, 4, 6, 8 and
10 g /kg diet respectively. The study was lasted for 42 days. Average body weight, weight gain, relative growth rate, feed
conversion, antibody titer against Newcastle disease, phagocytic activity and phagocytic index, some blood parameters(GOT, GPT,
Glucose, Cholesterol, Triglyceride, Total protein, Albumen, WBCs,
RBCs, Hb and PCV), dressing percentage, weight of different body
organs, abdominal fat weight, were determined. It was found that, N. Sativa significantly improved final body weight, total body gain
and feed conversion ratio of groups 2 and 3 when compared with the control group. Higher levels of N. Sativa did not improve
growth performance of the chicks. Non significant differences were
observed for antibody titer against Newcastle virus, WBCs count,
serum GOT, glucose level, dressing %, relative liver, spleen, heart and head percentages. Lymphoid organs (Bursa and Thymus)
improved significantly with increasing N. Sativa level in all supplemented groups. Serum cholesterol, triglyceride and visible fat
% significantly decreased with Nigella sativa supplementation while
serum GPT level significantly increased with nigella sativa
supplementation.
Abstract: Understanding the cell's large-scale organization is an interesting task in computational biology. Thus, protein-protein interactions can reveal important organization and function of the cell. Here, we investigated the correspondence between protein interactions and function for the yeast. We obtained the correlations among the set of proteins. Then these correlations are clustered using both the hierarchical and biclustering methods. The detailed analyses of proteins in each cluster were carried out by making use of their functional annotations. As a result, we found that some functional classes appear together in almost all biclusters. On the other hand, in hierarchical clustering, the dominancy of one functional class is observed. In the light of the clustering data, we have verified some interactions which were not identified as core interactions in DIP and also, we have characterized some functionally unknown proteins according to the interaction data and functional correlation. In brief, from interaction data to function, some correlated results are noticed about the relationship between interaction and function which might give clues about the organization of the proteins, also to predict new interactions and to characterize functions of unknown proteins.
Abstract: Annotation of a protein sequence is pivotal for the understanding of its function. Accuracy of manual annotation provided by curators is still questionable by having lesser evidence strength and yet a hard task and time consuming. A number of computational methods including tools have been developed to tackle this challenging task. However, they require high-cost hardware, are difficult to be setup by the bioscientists, or depend on time intensive and blind sequence similarity search like Basic Local Alignment Search Tool. This paper introduces a new method of assigning highly correlated Gene Ontology terms of annotated protein sequences to partially annotated or newly discovered protein sequences. This method is fully based on Gene Ontology data and annotations. Two problems had been identified to achieve this method. The first problem relates to splitting the single monolithic Gene Ontology RDF/XML file into a set of smaller files that can be easy to assess and process. Thus, these files can be enriched with protein sequences and Inferred from Electronic Annotation evidence associations. The second problem involves searching for a set of semantically similar Gene Ontology terms to a given query. The details of macro and micro problems involved and their solutions including objective of this study are described. This paper also describes the protein sequence annotation and the Gene Ontology. The methodology of this study and Gene Ontology based protein sequence annotation tool namely extended UTMGO is presented. Furthermore, its basic version which is a Gene Ontology browser that is based on semantic similarity search is also introduced.
Abstract: 16-Mercaptohexadecanoic acid (MHDA) and tripeptide glutathione conjugated with gold nanoparticles (Au-NPs) are characterized by Fourier Transform InfaRared (FTIR) spectroscopy combined with Surface-enhanced Raman scattering (SERS) spectroscopy. Surface Plasmon Resonance (SPR) technique based on FTIR spectroscopy has become an important tool in biophysics, which is perspective for the study of organic compounds. FTIR-spectra of MHDA shows the line at 2500 cm-1 attributed to thiol group which is modified by presence of Au-NPs, suggesting the formation of bond between thiol group and gold. We also can observe the peaks originate from characteristic chemical group. A Raman spectrum of the same sample is also promising. Our preliminary experiments confirm that SERS-effect takes place for MHDA connected with Au-NPs and enable us to detected small number (less than 106 cm-2) of MHDA molecules. Combination of spectroscopy methods: FTIR and SERS – enable to study optical properties of Au- NPs and immobilized bio-molecules in context of a bio-nano-sensors.
Abstract: Bacterial magnetic nanoparticles have great useful potential in biotechnological and biomedical applications. In this study, a liquid growth medium was modified for cultivation a fastidious magnetotactic bacterium that has been isolated from Anzali
lagoon, Iran in our previous research. These modifications include
change in vitamin, mineral, carbon sources and etcetera. In our
experience, the serum bottles and designed air-tight laboratory bottles
were used to create microaerobic conditions in order to development
of a method for scale-up experiment. This information may serve as a
guide to green chemistry based biological protocols for the synthesis
of magnetic nanoparticles with control over the chemical
composition, morphology and size.
Abstract: A number of competing methodologies have been developed
to identify genes and classify DNA sequences into coding
and non-coding sequences. This classification process is fundamental
in gene finding and gene annotation tools and is one of the most
challenging tasks in bioinformatics and computational biology. An
information theory measure based on mutual information has shown
good accuracy in classifying DNA sequences into coding and noncoding.
In this paper we describe a species independent iterative
approach that distinguishes coding from non-coding sequences using
the mutual information measure (MIM). A set of sixty prokaryotes is
used to extract universal training data. To facilitate comparisons with
the published results of other researchers, a test set of 51 bacterial
and archaeal genomes was used to evaluate MIM. These results
demonstrate that MIM produces superior results while remaining
species independent.
Abstract: In this research, a biofiltration process to remove
ammonia gas from gas stream using agricultural residue biofilter
medias is studied. The experiments were conducted in laboratoryscale
biofilter. The biofilter medias were a mixture of manure
fertilizer and bagasse at various ratios i.e., 1:3, 1:5 and 1:7. The
experiments were performed for a period of 40 days. The empty bed
retention time (EBRT) is 78s. The moisture content of biofilter media
was maintained at 45-60% using water. The results showed that the
agricultural residues (manure fertilizer and bagasse) are suitable as
biofilter media for ammonia gas removal in biofiltration process.
The maximum efficiency of ammonia gas removal is observed
from the 1:5 of manure fertilizer: bagasse ratio at 89.93%. The
biofiltration is more effective at low ammonia gas concentration. In
addition, the mixture ratio of biofilter media is not a significant factor
in biofiltration operation while the most significant factor for
biofiltration operation is the inlet ammonia gas concentration.
Abstract: Hybridization refers to the crossing breeding of two
plants. Coefficient of Parentage (COP) is used by the plant breeders
to determine the genetic diversity across various varieties so as to
incorporate the useful characters of the two varieties to develop a
new crop variety with particular useful characters. Genetic Diversity
is the prerequisite for any cultivar development program. Genetic
Diversity depends upon the pedigree information of the varieties
based on particular levels. Pedigree refers to the parents of a
particular variety at various levels. This paper discusses the searching
and analyses of different possible pairs of varieties selected on the
basis of morphological characters, Climatic conditions and Nutrients
so as to obtain the most optimal pair that can produce the required
crossbreed variety. An algorithm was developed to determine the
coefficient of parentage (COP) between the selected wheat varieties.
Dummy values were used wherever actual data was not available.
Abstract: A game animals – elk (Alces alces), deer (Cervus
elaphus), roe deer (Capreolus capreolus) or wild boar (Sus scrofa
scrofa) - every autumn and winter period provide an excellent
investment, diversification of many consumer meals. In last years
consumption and assortiment of game meat products significantly
increase. Investigations about biochemical composition of game meat
are not very much. The meat of wild animals is more favourable for
human health because it has lower saturated fatty acids content, but
higher content of protein. Therefore the aim of investigations was to
compare biochemical composition of ungulates obtained in
Latvia.Investigations were carried out in wild animals different
regions of Latvia. In the studied samples protein, intramuscular fat,
fatty acids and cholesterol were determined. The biochemical
analysis of 54 samples were done. Results of analysis showed that
protein content 22.36 – 22.92% of all types of meat samples is not
different statistically, significantly lower fat content 1.33 ± 0.88%
had elk meat samples and 1.59 ± 0.59% roe deer samples. Content of
cholesterol was various 64.41 – 95.07% in the ruminant meat
samples of different species. From the dietetic point of view the best
composition of fatty acids has meat samples of roe deer.
Abstract: Microbial oil was produced by soil isolated
oleaginous yeast YU5/2 in flask-batch fermentation. The yeast was
identified by molecular genetics technique based on sequence
analysis of the variable D1/D2 domain of the large subunit (26S)
ribosomal DNA and it was identified as Torulaspora globosa. T.
globosa YU5/2 supported maximum values of 0.520 g/L/d, 0.472 g
lipid/g cells, 4.16 g/L, and 0.156 g/L/d for volumetric lipid
production rate, and specific yield of lipid, lipid concentration, and
specific rate of lipid production respectively, when culture was
performed in nitrogen-limiting medium supplemented with 80g/L
glucose. Among the carbon sources tested, maximum cell yield
coefficient (YX/S, g/L), maximum specific yield of lipid (YP/X, g
lipid/g cells) and volumetric lipid production rate (QP, g/L/d) were
found of 0.728, 0.237, and 0.619, respectively, using sweet potato
tubers hydrolysates as carbon source.
Abstract: Twenty four New Zealand white rabbits (12 does and
12 bucks) and twenty four Flanders (12 does and 12 bucks) rabbits,
allotted into two feeding regime (6 for each breed, 3 males and 3
females) first one fed commercial ration and second one fed
commercial diet plus sodium butyrate (300 g/ton). The obtained
results showed that at end of 8th week experimental period New
Zealand white rabbits were heavier body weight than Flanders rabbits
(1934.55+39.05 vs. 1802.5+30.99 g); significantly high body weight
gain during experimental period especially during 8th week
(136.1+3.5 vs. 126.8+1.8 g/week); better feed conversion ratio during
all weeks of experiment from first week (3.07+0.16 vs. 3.12+0.10)
till the 8th week of experiment (5.54+0.16 vs. 5.76+0.07) with
significantly high dressing percentages (0.54+0.01 vs. 0.52+0.01).
Also all carcass cuts were significantly high in New Zealand white
rabbits than Flanders. Females rabbits (at the same age) were lower
body weight than males from start of experiment (941.1+39.8
vs.972.1+33.5 g) till the end of experiment (1833.64+37.69 vs.
1903.41+36.93 g); gained less during all weeks of experiment except
during 8th week (132.1+2.3 vs. 130.9+3.4 g/week), with lower
dressing percentage (0.52+0.01 vs. 0.53+0.01) and lighter carcass
cuts than males, however, they had better feed conversion ratio
during 1st week, 7th week and 8th week of experiment. Addition of
300g sodium butyrate/ton of rabbit increased the body weight of
rabbits at the end of experimental period (1882.71+26.45 vs.
1851.5+49.82 g); improve body weight gain at 3rd, 4th, 5th, 6th and
7th week of experiment and significantly improve feed conversion
ratio during all weeks of the experiment from 1st week (2.85+0.07
vs. 3.30+0.15) till the 8th week of the experiment (5.51+0.12 vs.
5.77+0.12). Also the dressing percentage was higher in Sodium
butyrate fed groups than control one (0.53+0.01 vs. 0.52+0.01) and
the most important results of feeding sodium butyrate is the reducing
of the mortality percentage in rabbits during 8 week experiment to
zero percentage as compared with 16% in control group.
Abstract: In conventional seedling production, the seedlings are
being grown in the open field under natural conditions. Here they are
susceptible to sudden changes in climate were their quality and yield
is affected. Quality seedlings are essential for good growth and
performance of crops in main field; they serve as a foundation for the
economic returns to the farmer. Producing quality seedling demands
usage of hybrid seeds as they have the ability to result in better yield,
greater uniformity, improved color, disease resistance, and so forth.
Hybrid seed production poses major operational challenge and its
seed use efficiency plays an important role. Thus in order to
overcome the difficulties currently present in conventional seedling
production and to efficiently use hybrid seeds, ITC Limited Agri
Business Divisions - Sustainability Cell as conceptualized a novel
method of seedling production unit for farmers in West Godavari
District of Andhra Pradesh. The “Green House based Float Seedling"
methodology aims at a protected cultivation technique wherein the
micro climate surrounding the plant/seedling body is controlled
partially or fully as per the requirement of the species. This paper
reports on the techno economic evaluation of green house for
cultivation of float based seedling production with experimental
results that was attained from the pilot implementation in West
Godavari District, Rajahmundry region of India.
Abstract: The phylogenetic analysis using the most conservative
portions of 18S rRNA gene revealed the phylogenetic relationship
among the two populations where DNA divergence showed that the
nucleotides diversity value were -0.00838 for the Tanjung Dawai,
Kedah and -0.00708 for the Cherating, Pahang populations
respectively. The net nucleotide divergence among populations (Da)
was -0.0073 indicating a low polymorphism among the populations
studied. Total number of mutations in the Tanjung Dawai, Kedah
samples was higher than Cherating, Pahang samples, which are 73 and
59 respectively while shared mutations across the populations were 8,
and reveal the evolutionary in the genome of Malaysian T. gigas. The
tree topology of both populations inferred using Neigbour-joining
method by comparing 1791 bp of partial 18S rRNA sequence revealed
that T. gigas haplotypes were clustered into seven clades, suggesting
that they are genetically diverse among populations which derived
from a common ancestor.
Abstract: Protein residue contact map is a compact
representation of secondary structure of protein. Due to the
information hold in the contact map, attentions from researchers in
related field were drawn and plenty of works have been done
throughout the past decade. Artificial intelligence approaches have
been widely adapted in related works such as neural networks,
genetic programming, and Hidden Markov model as well as support
vector machine. However, the performance of the prediction was not
generalized which probably depends on the data used to train and
generate the prediction model. This situation shown the importance
of the features or information used in affecting the prediction
performance. In this research, support vector machine was used to
predict protein residue contact map on different combination of
features in order to show and analyze the effectiveness of the
features.
Abstract: The effect of phosphorus supplementation of ammoniated rice straw was studied. The in vitro experiment was carried out following the first stage of Tilley and Terry method. The treatments consisting of four diets were A = 50% ammoniated rice straw + 50% concentrate (control), B = A + 0.2% Phosphor (P) supplement, C = A + 0.4% Phosphor (P) supplement, and D = A + 0.6% Phosphor (P) supplement of dry matter. Completely randomized design was used as the experimental design with differences among treatment means were examined using Duncan multiple range test. Variables measured were total bacterial and cellulolytic bacterial population, cellulolytic enzyme activity, ammonia (NH3) and volatile fatty acid (VFA) concentrations, as fermentability indicators and synthesized microbial protein, as well as degradability indicators including dry matter (DM), organic matter (OM), neutral detergent fibre (NDF), acid detergent fibre (ADF) and cellulose. The results indicated that fermentability and degradability of diets consisting ammoniated rice straw with P supplementation were significantly higher than the control diet (P< 0.05). It is concluded that P supplementation is important to improve fermentability and degradability of rations containing ammoniated RS and concentrate. In terms of the most effective level of P supplementation occurred at a supplementation rate of 0.4% of dry matter.
Abstract: The aim of this work was to detect genetic variability among the set of 40 castor genotypes using 8 RAPD markers. Amplification of genomic DNA of 40 genotypes, using RAPD analysis, yielded in 66 fragments, with an average of 8.25 polymorphic fragments per primer. Number of amplified fragments ranged from 3 to 13, with the size of amplicons ranging from 100 to 1200 bp. Values of the polymorphic information content (PIC) value ranged from 0.556 to 0.895 with an average of 0.784 and diversity index (DI) value ranged from 0.621 to 0.896 with an average of 0.798. The dendrogram based on hierarchical cluster analysis using UPGMA algorithm was prepared and analyzed genotypes were grouped into two main clusters and only two genotypes could not be distinguished. Knowledge on the genetic diversity of castor can be used for future breeding programs for increased oil production for industrial uses.