International Journal of Biological, Life and Agricultural Sciences

Study of Compost Maturity during Humification Process using UV-Spectroscopy

Year: 2011 Volume: 5 Issue: 8 486 - 488 Pages

Abstract: The increments of aromatic structures are widely used to monitor the degree of humification. Compost derived from mix manures mixed with agricultural wastes was studied. The compost collected at day 0, 7, 14, 21, 28, 35, 49, 77, 91, 105, and 119 was divided into 3 stages, initial stage at day 0, thermophilic stage during day 1-48, and mature stage during day 49-119. The change of highest absorptions at wavelength range between 210-235 nm during day 0- 49 implied that small molecules such as nitrates and carboxylic occurred faster than the aromatic molecules that were found at wavelength around 280 nm. The ratio of electron-transfer band at wavelength 253 nm by the benzonoid band at wavelength 230 nm (E253/E230) also gradually increased during the fermenting period indicating the presence of O-containing functional groups. This was in agreement with the shift change from aliphatic to aromatic structures as shown by the relationship with C/N and H/C ratios (r = - 0.631 and -0.717, p< 0.05) since both were decreasing. Although the amounts of humic acid (HA) were not different much during the humification process, the UV spectral deconvolution showed better qualitative characteristics to help in determining the compost quality. From this study, the compost should be used at day 49 and should not be kept longer than 3 months otherwise the quality of HA would decline regardless of the amounts of HA that might be rising. This implied that other processes, such as mineralization had an influence on the humification process changing HA-s structure and its qualities.

Effect of Processing on Sensory Characteristics and Chemical Composition of Cottonseed (Gossypium hirsutum) and Its Extract

Year: 2013 Volume: 7 Issue: 3 226 - 230 Pages

Abstract: The seeds of cotton (Gossypium hirsutum) fall among the lesser known oil seeds. Cottonseeds are not normally consumed in their natural state due to their gossypol content, an antinutrient. The effect of processing on the sensory characteristics and chemical composition of cottonseed and its extract was studied by subjecting the cottonseed extract to heat treatment (boiling) and the cottonseed to fermentation. The cottonseed extract was boiled using the open pot and the pressure pot for 30 minutes respectively. The fermentation of the cottonseed was carried out for 6 days with samples withdrawn at intervals of 2 days. The extract and fermented samples were subjected to chemical analysis and sensory evaluated for colour, aroma, taste, mouth feel, appearance and overallacceptability. The open pot sample was more preferred. Fermentation for 6 days resulted into a significant reduction in gossypol level of the cottonseed; however, sample fermented for 2 days was most preferred.

Molecular Docking Studies of Mycobacterium tuberculosis RNA Polymerase β Subunit (rpoB) Receptor

Year: 2013 Volume: 7 Issue: 5 317 - 322 Pages

Abstract: Tuberculosis (TB) is a bacterial infectious disease caused by the obligate human pathogen, Mycobacterium tuberculosis. Multidrug-resistant tuberculosis (MDR-TB) is a global reality that threatens tuberculosis control. Resistance to antibiotic Rifampicin, occurs in 95% of cases through nucleotide substitutions in an 81-bp core region of the rpoB i.e; beta subunit of DNA dependant RNA polymerase. In this paper, we studied the Rifampicin-rpoB receptor interactions In silico. First, homology modeling was performed to obtain the three dimensional structure of Mycobacterium rpoB. Sixty analogs of Rifampicin were prepared using Marvin sketch software. Both original Rifampicin and the analogs were docked with rpoB and energy values were obtained. Out of sixty analogs, 43 analogs had lesser energy values than conventional Rifampicin and hence are predicted to have greater binding affinity to rpoB. Thus, this study offers a route for the development of Rifampicin analogs against multi drug resistant Mycobacterium rpoB.

Inhibition of the Growth of Pathogenic Candida spp. by Salicylhydroxamic Acid

Year: 2010 Volume: 4 Issue: 1 109 - 115 Pages

Abstract: Candida spp. are common and aggressive pathogens. Because of the growing resistance of Candida spp. to current antifungals, novel targets, found in Candida spp. but not in humans or other flora, have to be identified. The alternative oxidase (AOX) is one such possibility. This enzyme is insensitive to cyanide, but is sensitive to compounds such as salicylhydroxamic acid (SHAM), disulfiram and n-alkyl gallates. The growth each of six Candida spp. was inhibited significantly by ~13 mM SHAM or 2 mM cyanide, albeit to differing extents. In C. dubliniensis, C. krusei and C. tropicalis the rate of O2 uptake was inhibited by 18-36% by 25 mM SHAM, but this had little or no effect on C. glabrata, C. guilliermondii or C. parapsilosis. Although SHAM substantially inhibited the growth of Candida spp., it is unlikely that the inhibition of AOX was the cause. Salicylhydroxamic acid is used therapeutically in the treatment of urinary tract infections and urolithiasis, but it also has some potential in the treatment of Candida spp. infection.

Effect of Retinoic Acid on Fetus Reproductive Organ Mice (Mus musculus) Swiss Webster

Year: 2012 Volume: 6 Issue: 4 201 - 203 Pages

Authors:
Yulia Irnidayanti

Abstract: Retinoic acid is like a steroid hormone that plays a role in embryo formation, proliferation of spermatogonia cells, ephitelial cells differentiation and organogenesis. Retinoic acid can influences seminiferous tubule formation during embryonic testis development and also play a role in the regulation of ovarian function and female reproductive tract by suppressing the hormones FSH receptor expression. The excessive use of retinoic acid caused abnormalities in the fetus. The result showed that there is the influence of retinoic acid on the developmet of mice fetal testes, for examples disruption of the formation of seminiferous tubules and tubules seemed to be hollow, spermatogonia cells are relatively few in number and caused Leydig cells count relatively more. While in the female fetus does not caused the formation of primordial follicles and disrupted the development of germinal ephitelial cells of fetal ovaries of female mice (mus musculus) Swiss Webster.

Chewing behavior and Bolus Properties as Affected by Different Rice Types

Year: 2012 Volume: 6 Issue: 1 40 - 45 Pages

Abstract: The study aimed to investigate the effect of rice types on chewing behaviours (chewing time, number of chews, and portion size) and bolus properties (bolus moisture content, solid loss, and particle size distribution (PSD)) in human subjects. Five cooked rice types including brown rice (BR), white rice (WR), parboiled white rice (PR), high amylose white rice (HR) and waxy white rice (WXR) were chewed by six subjects. The chewing behaviours were recorded and the food boluses were collected during mastication. Rice typeswere found to significantly influence all chewing parameters evaluated. The WXR and BR showed the most pronounced differences compared with other rice types. The initial moisture content of un-chewed WXR was lowest (43.39%) whereas those of other rice types were ranged from 66.86 to 70.33%. The bolus obtained from chewing the WXR contained lowest moisture content (56.43%) whilst its solid loss (22.03%) was not significant different from those of all rice types. In PSD evaluation using Mastersizer S, the diameter of particles measured was ranged between 4 to 3500 μm. The particle size of food bolus from BR, HR, and WXR contained much finer particles than those of WR and PR.

Influence of Drought on Yield and Yield Components in White Bean

Year: 2011 Volume: 5 Issue: 7 395 - 404 Pages

Authors:
Gholamreza Habibi

Abstract: In order to study seed yield and seed yield components in bean under reduced irrigation condition and assessment drought tolerance of genotypes, 15 lines of White beans were evaluated in two separate RCB design with 3 replications under stress and non stress conditions. Analysis of variance showed that there were significant differences among varieties in terms of traits under study, indicating the existence of genetic variation among varieties. The results indicate that drought stress reduced seed yield, number of seed per plant, biological yield and number of pod in White been. In non stress condition, yield was highly correlated with the biological yield, whereas in stress condition it was highly correlated with harvest index. Results of stepwise regression showed that, selection can we done based on, biological yield, harvest index, number of seed per pod, seed length, 100 seed weight. Result of path analysis showed that the highest direct effect, being positive, was related to biological yield in non stress and to harvest index in stress conditions. Factor analysis were accomplished in stress and nonstress condition a, there were 4 factors that explained more than 76 percent of total variations. We used several selection indices such as Stress Susceptibility Index ( SSI ), Geometric Mean Productivity ( GMP ), Mean Productivity ( MP ), Stress Tolerance Index ( STI ) and Tolerance Index ( TOL ) to study drought tolerance of genotypes, we found that the best Stress Index for selection tolerance genotypes were STI, GMP and MP were the greatest correlations between these Indices and seed yield under stress and non stress conditions. In classification of genotypes base on phenotypic characteristics, using cluster analysis ( UPGMA ), all allels classified in 5 separate groups in stress and non stress conditions.

The Evaluation and the Comparison of the Effect of Without Engine Power and Power Mechanical Systems on Rice Weed

Year: 2012 Volume: 6 Issue: 3 96 - 101 Pages

Abstract: In order to study the influence of different methods of controlling weeds such as mechanical weeding and mechanical weeder efficiency analysis in mechanical cultivation conditions, in farming year of 2011 an experiment was done in a farm in coupling and development of technology center in Haraz,Iran. The treatments consisted of (I) control treatment: where no weeding was done, (II) use of mechanical weeding without engine and (III) power mechanical weeding. Results showed that experimental treatments had significantly different effects (p=0.05) on yield traits and number of filled grains per panicle, while treatments had the significant effects on grain weight and dry weight of weeds in the first, second and third weeding methods at 1% of confidence level. Treatment (II) had its most significant effect on number of filled grains per panicle and yield performance standpoint, which was 3705.97 kg ha-1 in its highest peak. Treatment (III) was ranked as second influential with 3559.8 kg ha-1. In addition, under (I) treatments, 2364.73 kg ha-1 of yield produced. The minimum dry weights of weeds in all weeding methods were related to the treatment (II), (III) and (I), respectively. The correlation coefficient analysis showed that total yield had a significant positive correlation with the panicle grain yield per plant (r= 0.55*) and the number of grains per panicle-1 (r= 0.57*) and the number of filled grains (r= 0.63*). Total rice yield also had negative correlation of r= -0. 64* with weed dry weight at second weed sampling time (17 DAT). The weed dry weight at third and fourth sampling times (24 and 40 DAT) had negative correlations of -0.65** and r=-0.61* with rice yield, respectively.

Study of the Sorption of Biosurfactants from l. Pentosus on Sediments

Year: 2011 Volume: 5 Issue: 7 390 - 394 Pages

Abstract: Losses of surfactant due to sorption need to be considered when selecting surfactant doses for soil bioremediation. The degree of surfactant sorption onto soil depends primarily on the organic carbon fraction of soil and the chemical nature of the surfactant. The use of biosurfactants in the control of the bioavailability of toxicants in soils is an attractive option because of their biodegradability. In this work biosurfactants were produced from a cheap raw material, trimming vine shoots, employing Lactobacillus pentosus. When biosurfactants from L. pentosus was added to sediments the surface tensión of the water containing the sediments rapidly increase, the same behaviour was observed with the chemical surfactant Tween 20; whereas sodyum dodecyl sulphate (SDS) kept the surface tension of the water around 36 mN/m. It means, that the behaviour of biosurfactants from L. pentosus is more similar to non-ionic surfactatns than to anionic surfactants.

Some Physico-Chemical Characteristics and Mineral Contents of Gilaburu (Viburnum opulus L.) Fruits in Turkey

Year: 2013 Volume: 7 Issue: 6 443 - 445 Pages

Abstract: Gilaburu (Viburnum opulus L.) grown naturally in Anatolia. In this study, some physico-chemical (sugar, acid, protein, crude fat, crude fiber, ash etc.) characteristics and mineral composition of Gilaburu fruit have been investigated. The length, width, thickness, weight, total soluble solid, protein, crude ash, crude fiber and crude oil of fruit were found to be 1.12 cm, 1.58 cm, 1.87 cm, 0.87 g, 14.73 %, 0.2 %, 0.11 %, 6.56 % and 0.4 %, respectively. The seed of fruit mean weight, length, width and thickness were determinated as 0.08 g, 7.76 cm, 7.67 cm and 1.66, respectively. In addition 27 mineral elements (Al, Mg, Na, Ba, Ca, Ni, Cd, P, Cr, Pb, S, Cu, Se, Fe, K, Sr, Li, Z, V, Ag, Bi, Co, Mn, B, Ga, In, Ti) were analyzed. Gilaburu (Viburnum opulus L.) fruit was richest in potassium (10764.764 ppm), Mg (1289.088 ppm) and P (1304.169 ppm).

Reducing Sugar Production from Durian Peel by Hydrochloric Acid Hydrolysis

Year: 2012 Volume: 6 Issue: 9 781 - 786 Pages

Abstract: Agricultural waste is mainly composed of cellulose and hemicelluloses which can be converted to sugars. The inexpensive reducing sugar from durian peel was obtained by hydrolysis with HCl concentration at 0.5-2.0% (v/v). The hydrolysis range of time was for 15-60 min when the mixture was autoclaved at 121 °C. The result showed that acid hydrolysis efficiency (AHE) highest to 80.99% at condition is 2.0%concentration for 15 min. Reducing sugar highest to 56.07 g/litre at condition is 2.0% concentration for 45min. Total sugar highest to 59.83 g/litre at condition is 2.0%concentration for 45min, which was not significant (p < 0.05) with condition 2.0% concentration for 30 min and 1.5 % concentration for 45 and 60 min. The increase in concentration increased AHE, reducing sugar and total sugar. The hydrolysis time had no effect on AHE, reducing sugar and total sugar. The maximum reducing sugars of each concentration were at hydrolysis time 45 min .The hydrolysated were analysis by HPLC, the results revealed that the principle of sugar were glucose, fructose and xylose.

An Efficient Protocol for Cyclic Somatic Embryogenesis in Neem (Azadirachta indica A Juss.)

Year: 2009 Volume: 3 Issue: 3 162 - 164 Pages

Abstract: Neem is a highly heterozygous and commercially important perennial plant. Conventionally, it is propagated by seeds which loose viability within two weeks. Strictly cross pollinating nature of the plant causes serious barrier to the genetic improvement by conventional methods. Alternative methods of tree improvement such as somatic hybridization, mutagenesis and genetic transformation require an efficient in vitro plant regeneration system. In this regard, somatic embryogenesis particularly secondary somatic embryogenesis may offer an effective system for large scale plant propagation without affecting the clonal fidelity of the regenerants. It can be used for synthetic seed production, which further bolsters conservation of this tree species which is otherwise very difficult The present report describes the culture conditions necessary to induce and maintain repetitive somatic embryogenesis, for the first time, in neem. Out of various treatments tested, the somatic embryos were induced directly from immature zygotic embryos of neem on MS + TDZ (0.1 μM) + ABA (4 μM), in more than 76 % cultures. Direct secondary somatic embryogenesis occurred from primary somatic embryos on MS + IAA (5 μM) + GA3 (5 μM) in 12.5 % cultures. Embryogenic competence of the explant as well as of the primary embryos was maintained for a long period by repeated subcultures at frequent intervals. A maximum of 10 % of these somatic embryos were converted into plantlets.

Effect of Process Parameters on Aerobic Decolourization of Reactive Azo Dye using Mixed Culture

Year: 2009 Volume: 3 Issue: 10 530 - 533 Pages

Abstract: In the present study, an attempt was made to examine the potential of aerobic mixed culture for decolourization of Remazol Black B dye in batch reactors. The effect of pH, temperature, inoculum, initial concentration of dye and initial concentration of glucose was studied with an aim to determine the optimal conditions required for maximum decolourization and degradation. The culture exhibited maximum decolourization ability at pH between 7-8 and at 30°C. A 10% (v/v) inoculum and 1% (w/v) glucose concentration were found to be the optimum for decolourization. A maximum of 98% decolourization was observed at 25 ppm initial concentration of dye after 18 hours of incubation period. At higher dye concentration of 300 ppm, the removal in colour was found to be 75% in 48 hours of incubation period. The results show that the enriched mixed culture from activated sludge has good potential in removal of Remazol Black B dye from wastewater under aerobic conditions.

Production of the Protein-Vitamin Complex from Wheat Germ

Year: 2013 Volume: 7 Issue: 7 690 - 693 Pages

Abstract: Wheat germ has a balanced amino acid composition of the protein, which is well digested by enzymes in the gastrointestinal tract of humans, a high content of vitamins, minerals and unsaturated acids. Introduction components grain food products will enrich their biologically important substances, giving these products a number of valuable properties and reducing their caloric. A complex natural system of substances in foods will help replenish the body's need of essential nutrients, increasing its resistance to the harmful effects of the environment, prolong life. In this regard, there was a need for the development of production technology of protein complexes from wheat germ and then applying them in food, particularly in the dairy industry. Experimental studies were conducted to determine the number of herbal supplements on the sensory characteristics of the product. Studies have been conducted to determine the optimal process parameters of water activity and moisture content of the investigational product.

Comparative Analysis of Farm Enterprises Performance in Two Agro-Ecological Feuding Zone of Nigeria

Year: 2013 Volume: 7 Issue: 1 63 - 67 Pages

Abstract: The two agro-ecological zones became the focus of the study because of violent nature of the incessant conflict in the zones. The available register of farmers association was the sampling frame work where ten percent (61) farmers per state were randomly sampled. Data were collected and analysed using z-test. The research findings revealed tree crops and grains production enterprises ranked higher in Osun (rain fed zones) and Taraba states (savannah zones) respectively. Osun state entrepreneur felt the effect of the conflict on their enterprises more than Tarba state. The reasons adduced for severity of the conflict on enterprises are majority (77.0%) migrated and (75.5%) of them were not allowed to enter their farms during and when conflict deescalated unlike situation in Taraba state. The different in enterprises production level between the two agroecological zone was statistically significant at p

Effects of Safflower Cake Dietary Supplementation on Growth Performances, Carcass Traits and Meat Quality of Garganica Kids

Year: 2011 Volume: 5 Issue: 6 362 - 364 Pages

Abstract: Two group of kids (“Safflower cake" and “Control") were fed ad libitum with pelleted total mixed rations. After a 7-days adaptation period, the diet of the “Safflower cake" group were supplemented with 20% of safflower cake. The kids were slaughtered at 96 days of age. Dietary safflower cake did not affect the growth traits of kids. In addition, kids fed experimental diet showed a lower feed intake and consequently a better feed conversion ratio in comparison to the “Control" group. The use of safflower decreased the level of SFA and increased the level of MUFA in kid meat. The level of PUFA was higher in lipid extracted from animals feeding “Control“ diet even if the UFA level was lower. Furthermore, lipid extracted from animals feeding control diet contained more ω6 fatty acids in comparison to kids feeding experimental diet while the opposite trend was observed for the level of ω3 fatty acids. The ω6 to ω3 ratio was significantly affected by diet and in particular this ratio decreased in meat of kids fed experimental diet. Our results indicate that intramuscular fatty acid composition of kid meat can be improved from a human health perspective by inclusion of safflower cake in the diet.

Sustainable Production of Oyster Mushroom (Pleurotus ostreatus) in Chiapas, Mexico

Year: 2013 Volume: 7 Issue: 7 686 - 689 Pages

Abstract: Pleurotus ostreatus is a common edible mushroom with a number of properties that can help to solve the nutritional and economical problems of people in Chiapas, Mexico. The objective of this project was to produce the mushroom under a sustainable management in which only regional products were allowed as a way to promote the cultivation and consumption of Pleurotus ostreatus; 5 different substrates were tested as well as 2 sanitation methods. The obtained results showed that the highest yields were obtained using corn husk and a thermal sanitation method. Pests and diseases were not a problem during the project but they appeared more in the substrates sanitized with calcium hydroxide.

Response of BGA-Urea Fertigation as N2 Source on Growth Parameters and Yield of Paddy (Oryza sativa L.) in Agra (India)

Year: 2013 Volume: 7 Issue: 7 683 - 685 Pages

Abstract: Paddy being cultivated since about 10,000 years B.C in Ganga Valley in India, its production reached up to 99 million tons in the year 2012. BGA are of much ecological importance for maintaining the soil fertility and reclaiming the alkalinity. In present investigation attempts were made to identify the local cyanobacterial genera from the paddy fields, BGA application for green farming enabling the paddy to utilize more amount of nitrogen released and to examine its impact along with Urea upon growth and yield responses of the Paddy crop. It was observed that combined treatment of BGA with Urea proved better response in almost all growth parameters and yield attributes except number of tillers/ Plant and grains/ panicle as compared to application of either Urea or BGA alone. The Paddy growers should be encouraged to adopt BGA along with Urea as source of Nitrogen for Paddy cultivation.

Investigation of Growth Parameters of Soybean Cultivars in Different Weeding Regimes

Year: 2012 Volume: 6 Issue: 9 776 - 780 Pages

Abstract: In a field experiment, growth parameters of soybean cultivars in different weeding regimes was investigated. The trial was split plot in a randomized complete block design. The four cultivars and two lines of soybean (Glycine max L.) including: Sahar, Hill, Sari, Telar, 032 and 033 in main plot and weeding regime consist of no weeding (control), one weeding (35 days after planting) and two weeding (35+20 days after planting) were randomized in sub plot. The results showed that during the growth season 033 had the highest dry matter in two weeding. In two weeding regime the dry matter decreased. ). In all weeding regimes 033 had the highest CGR (Figs. 3a, 3b and 3c), which cleared this cultivar ability compare to the others. This cultivar by increasing its leaf area could do more photosynthesis, so, have a higher CGR.

A Comparative Study on Survival and Growth of Larvivorous Fish, Rasbora daniconius, Puntius ticto, and Puntius Conchonius

Year: 2014 Volume: 8 Issue: 2 108 - 111 Pages

Abstract: Experiments were carried out on the survival and growth of Rasbora daniconius, Puntius ticto and Puntius conchonius. The motivation of the study was to obtain information for growing the fish on a commercial scale for their use as biological control agents against mosquito larvae. The effects of temperature, total hardness, DO, pH and feed on the growth of fish were also investigated. Excessive value of total hardness was found because very rich calcium ion is present in Chitrakoot area. There was significant increases in growth rates of fish as temperature was increased from 280C to 300C. Further increases in temperature up to 320C, did not further affect growth. The positive and highly significant correlations 0.991488, 0.9581 and 0.9935 were found between length and weight of P. ticto, P. conchonius and R. daniconius respectively. The regression was significant at 5% level of probability.