Scholarly

In vitro Control of Aedes aegypti Larvae Using Beauveria bassiana

Year: 2018 Volume: 12 Issue: 10 400 - 404 Pages

Abstract: Aedes aegypti larval survival rate was assessed after exposure to blastopores or conidia (mineral oil-in-water formulation or aqueous suspension) of Beauveria bassiana CG 479 propagules (blastospores or conidia). Here, mineral oil was used in the fungal formulation to control Aedes aegypti larvae. 1%, 0.5% or 0.1% mineral oil-in-water solutions were used to evaluate mineral oil toxicity for mosquito larvae. In the oil toxicity test, 0.1% mineral oil solution reduced only 4.5% larval survival; accordingly, this concentration was chosen for fungal oil-in-water formulations. Aqueous suspensions were prepared using 0.01% Tween 80® in sterile dechlorinated water. A. aegypti larvae (L2) were exposed in aqueous suspensions or mineral oil-in-water fungal formulations at 1×107 propagules mL-1; the survival rate (assessed daily, for 7 days) and the median survival time (S50) were calculated. Seven days after the treatment, mosquito larvae survival rates were 8.56%, 16.22%, 58%, and 42.56% after exposure to oil-in-water blastospores, oil-in-water conidia, blastospores aqueous suspension and conidia aqueous suspension (respectively). Larvae exposed to 0.01% Tween 80® had 100% survival rate and the ones treated with 0.1% mineral oil-in-water had 95.11% survival rate. Larvae treated with conidia (regardless the presence of oil) or treated with blastospores formulation had survival median time (S50) ranging from one to two days. S50 was not determined (ND) when larvae were exposed to blastospores aqueous suspension, 0.01% Tween 80® (aqueous control) or 0.1% mineral oil-in-water formulation (oil control). B. bassiana conidia and blastospores (mineral oil-in-water formulated or suspended in water) had potential to control A. aegypti mosquito larvae, despite mineral oil-in-water formulation yielded better results in comparison to aqueous suspensions. Here, B. bassiana CG 479 isolate is suggested as a potential biocontrol agent of A. aegypti mosquito larvae.

Stability Analysis of a Human-Mosquito Model of Malaria with Infective Immigrants

Year: 2017 Volume: 11 Issue: 2 89 - 93 Pages

Abstract: In this paper, we analyse the stability of the SEIR model of malaria with infective immigrants which was recently formulated by the authors. The model consists of an SEIR model for the human population and SI Model for the mosquitoes. Susceptible humans become infected after they are bitten by infectious mosquitoes and move on to the Exposed, Infected and Recovered classes respectively. The susceptible mosquito becomes infected after biting an infected person and remains infected till death. We calculate the reproduction number R0 using the next generation method and then discuss about the stability of the equilibrium points. We use the Lyapunov function to show the global stability of the equilibrium points.

Ports and Airports: Gateways to Vector-Borne Diseases in Portugal Mainland

Year: 2016 Volume: 10 Issue: 5 258 - 263 Pages

Abstract: Vector-borne diseases are transmitted to humans by mosquitos, sandflies, bugs, ticks, and other vectors. Some are re-transmitted between vectors, if the infected human has a new contact when his levels of infection are high. The vector is infected for lifetime and can transmit infectious diseases not only between humans but also from animals to humans. Some vector borne diseases are very disabling and globally account for more than one million deaths worldwide. The mosquitoes from the complex Culex pipiens sl. are the most abundant in Portugal, and we dispose in this moment of a data set from the surveillance program that has been carried on since 2006 across the country. All mosquitos’ species are included, but the large coverage of Culex pipiens sl. and its importance for public health make this vector an interesting candidate to assess risk of disease amplification. This work focus on ports and airports identified as key areas of high density of vectors. Mosquitoes being ectothermic organisms, the main factor for vector survival and pathogen development is temperature. Minima and maxima local air temperatures for each area of interest are averaged by month from data gathered on a daily basis at the national network of meteorological stations, and interpolated in a geographic information system (GIS). The range of temperatures ideal for several pathogens are known and this work shows how to use it with the meteorological data in each port and airport facility, to focus an efficient implementation of countermeasures and reduce simultaneously risk transmission and mitigation costs. The results show an increased alert with decreasing latitude, which corresponds to higher minimum and maximum temperatures and a lower amplitude range of the daily temperature.

Dengue Death Review: A Tool to Adjudge the Cause of Dengue Mortality and Use of the Tool for Prevention of Dengue Deaths

Year: 2015 Volume: 9 Issue: 12 863 - 866 Pages

Abstract: Dengue is a mosquito-borne viral disease endemic in many countries in the tropics and sub-tropics. The state of Punjab in India shows cyclical and seasonal variation in dengue cases. The Case Fatality Rate of Dengue has ranged from 0.6 to 1.0 in the past years. The department has initiated review of the cases that have died due to dengue in order to know the exact cause of the death in a case of dengue. The study has been undertaken to know the other associated co-morbidities and factors causing death in a case of dengue. The study used the predesigned proforma on which the records (medical and Lab) were recorded and reviewed by the expert committee of the doctors. This study has revealed that cases of dengue having co-morbidities have longer stay in hospital. Fluid overload and co-morbidities have been found as major factors leading to death, however, in a confirmed case of dengue hepatorenal shutdown was found to be major cause of mortality. The data obtained will help in sensitizing the treating physicians in order to decrease the mortality due to dengue in future.

A DNA-Based Nanobiosensor for the Rapid Detection of the Dengue Virus in Mosquito

Year: 2015 Volume: 9 Issue: 12 832 - 835 Pages

Abstract: This paper describes the development of a DNA-based nanobiosensor to detect the dengue virus in mosquito using electrically active magnetic (EAM) nanoparticles as concentrator and electrochemical transducer. The biosensor detection encompasses two sets of oligonucleotide probes that are specific to the dengue virus: the detector probe labeled with the EAM nanoparticles and the biotinylated capture probe. The DNA targets are double hybridized to the detector and the capture probes and concentrated from nonspecific DNA fragments by applying a magnetic field. Subsequently, the DNA sandwiched targets (EAM-detector probe– DNA target–capture probe-biotin) are captured on streptavidin modified screen printed carbon electrodes through the biotinylated capture probes. Detection is achieved electrochemically by measuring the oxidation–reduction signal of the EAM nanoparticles. Results indicate that the biosensor is able to detect the redox signal of the EAM nanoparticles at dengue DNA concentrations as low as 10 ng/μl.

Effect of Pollination on Qualitative Characteristics of Rapeseed (Brassica campestris L. var. toria) Seed in Chitwan, Nepal

Year: 2014 Volume: 8 Issue: 12 1403 - 1406 Pages

Abstract: An experiment was conducted to determine the effect of pollination on seed quality of rapeseed in Chitwan, Nepal during 2012-2013. The experiment was designed in Randomized Complete Block with four replications and five treatments. The rapeseed plots were caged with mosquito nets at 10% flowering except natural pollination. Two-framed colonies of Apis mellifera L. and Apis cerana F. were introduced separately for pollination, and control plot caged without pollinators. The highest germination percent was observed on Apis cerana F. pollinated plot seeds (90.50% germination) followed by Apis mellifera L. pollinated plots (87.25 %) and lowest on control plots (42.00% germination) seeds. Similarly, seed test weight of Apis cerana F. pollinated plots (3.22 gm/ 1000 seed) and Apis mellifera L. pollinated plots (2.93 gm/1000 seed) were and lowest on control plots (2.26 gm/ 1000 seed) recorded. Likewise, oil content was recorded highest on pollinated by Apis cerana F. (36.1%) followed by pollinated by Apis mellifera L. (35.4%) and lowest on control plots (32.8%). This study clearly indicated pollination increases the seed quality of rapeseed and therefore, management of honeybee is necessary for producing higher quality of rapeseed under Chitwan condition.

Chikungunya Protease Domain–High Throughput Virtual Screening

Year: 2012 Volume: 6 Issue: 12 724 - 733 Pages

Abstract: Chikungunya virus (CHICKV) is an arboviruses belonging to family Tagoviridae and is transmitted to human through by mosquito (Aedes aegypti and Aedes albopictus) bite. A large outbreak of chikungunya has been reported in India between 2006 and 2007, along with several other countries from South-East Asia and for the first time in Europe. It was for the first time that the CHICKV outbreak has been reported with mortality from Reunion Island and increased mortality from Asian countries. CHICKV affects all age groups, and currently there are no specific drugs or vaccine to cure the disease. The need of antiviral agents for the treatment of CHICKV infection and the success of virtual screening against many therapeutically valuable targets led us to carry out the structure based drug design against Chikungunya nSP2 protease (PDB: 3TRK). Highthroughput virtual screening of publicly available databases, ZINC12 and BindingDB, has been carried out using the Openeye tools and Schrodinger LLC software packages. Openeye Filter program has been used to filter the database and the filtered outputs were docked using HTVS protocol implemented in GLIDE package of Schrodinger LLC. The top HITS were further used for enriching the similar molecules from the database through vROCS; a shape based screening protocol implemented in Openeye. The approach adopted has provided different scaffolds as HITS against CHICKV protease. Three scaffolds: Indole, Pyrazole and Sulphone derivatives were selected based on the docking score and synthetic feasibility. Derivatives of Pyrazole were synthesized and submitted for antiviral screening against CHICKV.

An Investigation on the Effect of Various Noises on Human Sensibility by using EEG Signal

Year: 2012 Volume: 6 Issue: 6 266 - 268 Pages

Abstract: Noise causes significant sensibility changes on a human. This study investigated the effect of five different noises on electroencephalogram (EEG) and subjective evaluation. Six human subjects were exposed to classic piano, ocean wave, alarm in army, ambulance, mosquito noise and EEG data were collected during the experimental session. Alpha band activity in the mosquito noise was smaller than that in the classic piano. Alpha band activity decreased 43.4 ± 8.2 % in the mosquito noise. On the other hand, Beta band activity in the mosquito noise was greater than that in the classic piano. Beta band activity increased 60.1 ± 10.7 % in the mosquito noise. The advances from this study may aid the product design process with human sensibility engineering. This result may provide useful information in designing a human-oriented product to avoid the stress.

A Comparative Study on Survival and Growth of Larvivorous Fish, Rasbora daniconius, Puntius ticto, and Puntius Conchonius

Year: 2014 Volume: 8 Issue: 2 108 - 111 Pages

Abstract: Experiments were carried out on the survival and growth of Rasbora daniconius, Puntius ticto and Puntius conchonius. The motivation of the study was to obtain information for growing the fish on a commercial scale for their use as biological control agents against mosquito larvae. The effects of temperature, total hardness, DO, pH and feed on the growth of fish were also investigated. Excessive value of total hardness was found because very rich calcium ion is present in Chitrakoot area. There was significant increases in growth rates of fish as temperature was increased from 280C to 300C. Further increases in temperature up to 320C, did not further affect growth. The positive and highly significant correlations 0.991488, 0.9581 and 0.9935 were found between length and weight of P. ticto, P. conchonius and R. daniconius respectively. The regression was significant at 5% level of probability.

Effect of Time Delay on the Transmission of Dengue Fever

Year: 2007 Volume: 1 Issue: 10 493 - 501 Pages

Abstract: The effect of a time delay on the transmission on dengue fever is studied. The time delay is due to the presence of an incubation period for the dengue virus to develop in the mosquito before the mosquito becomes infectious. The conditions for the existence of a Hopf bifurcation to limit cycle behavior are established. The conditions are different from the usual one and they are based on whether a particular third degree polynomial has positive real roots. A theorem for determining whether for a given set of parameter values, a critical delay time exist is given. It is found that for a set of realistic values of the parameters in the model, a Hopf bifurcation can not occur. For a set of unrealistic values of some of the parameters, it is shown that a Hopf bifurcation can occur. Numerical solutions using this last set show the trajectory of two of the variables making a transition from a spiraling orbit to a limit cycle orbit.

Maximizer of the Posterior Marginal Estimate for Noise Reduction of JPEG-compressed Image

Year: 2012 Volume: 6 Issue: 3 387 - 391 Pages

Abstract: We constructed a method of noise reduction for JPEG-compressed image based on Bayesian inference using the maximizer of the posterior marginal (MPM) estimate. In this method, we tried the MPM estimate using two kinds of likelihood, both of which enhance grayscale images converted into the JPEG-compressed image through the lossy JPEG image compression. One is the deterministic model of the likelihood and the other is the probabilistic one expressed by the Gaussian distribution. Then, using the Monte Carlo simulation for grayscale images, such as the 256-grayscale standard image “Lena" with 256 × 256 pixels, we examined the performance of the MPM estimate based on the performance measure using the mean square error. We clarified that the MPM estimate via the Gaussian probabilistic model of the likelihood is effective for reducing noises, such as the blocking artifacts and the mosquito noise, if we set parameters appropriately. On the other hand, we found that the MPM estimate via the deterministic model of the likelihood is not effective for noise reduction due to the low acceptance ratio of the Metropolis algorithm.

Mathematical Model for the Transmission of Two Plasmodium Malaria

Year: 2011 Volume: 5 Issue: 3 446 - 450 Pages

Authors:
P. Pongsumpun

Abstract: Malaria is transmitted to the human by biting of infected Anopheles mosquitoes. This disease is a serious, acute and chronic relapsing infection to humans. Fever, nausea, vomiting, back pain, increased sweating anemia and splenomegaly (enlargement of the spleen) are the symptoms of the patients who infected with this disease. It is caused by the multiplication of protozoa parasite of the genus Plasmodium. Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae and Plasmodium ovale are the four types of Plasmodium malaria. A mathematical model for the transmission of Plasmodium Malaria is developed in which the human and vector population are divided into two classes, the susceptible and the infectious classes. In this paper, we formulate the dynamical model of Plasmodium falciparum and Plasmodium vivax malaria. The standard dynamical analysis is used for analyzing the behavior for the transmission of this disease. The Threshold condition is found and numerical results are shown to confirm the analytical results.

New Effective Strains of Bacteria Bacillus thuringiensis ssp. israelensis for Bloodsucking Mosquito Control

Year: 2013 Volume: 7 Issue: 3 212 - 214 Pages

Abstract: Five original strains of entomopathogenic bacteria with insecticidal activity against mosquito larvae of the genera Aedes, Culex and Anopheles have been isolated from natural conditions in Armenia and characterized. According to morphological, physiological and biochemical parameters, all isolates were identified as Bacillus thuringiensis spp. israelensis (Bti). High larvicidal activity has been showed by three strains Bti. These strains can be recommended for industrial production of bacterial preparations.

ABURAS Index: A Statistically Developed Index for Dengue-Transmitting Vector Population Prediction

Year: 2007 Volume: 1 Issue: 5 55 - 58 Pages

Authors:
Hani M. Aburas

Abstract: “Dengue" is an African word meaning “bone breaking" because it causes severe joint and muscle pain that feels like bones are breaking. It is an infectious disease mainly transmitted by female mosquito, Aedes aegypti, and causes four serotypes of dengue viruses. In recent years, a dramatic increase in the dengue fever confirmed cases around the equator-s belt has been reported. Several conventional indices have been designed so far to monitor the transmitting vector populations known as House Index (HI), Container Index (CI), Breteau Index (BI). However, none of them describes the adult mosquito population size which is important to direct and guide comprehensive control strategy operations since number of infected people has a direct relationship with the vector density. Therefore, it is crucial to know the population size of the transmitting vector in order to design a suitable and effective control program. In this context, a study is carried out to report a new statistical index, ABURAS Index, using Poisson distribution based on the collection of vector population in Jeddah Governorate, Saudi Arabia.

Simulation Model for Predicting Dengue Fever Outbreak

Year: 2011 Volume: 5 Issue: 11 1331 - 1333 Pages

Abstract: Dengue fever is prevalent in Malaysia with numerous cases including mortality recorded over the years. Public education on the prevention of the desease through various means has been carried out besides the enforcement of legal means to eradicate Aedes mosquitoes, the dengue vector breeding ground. Hence, other means need to be explored, such as predicting the seasonal peak period of the dengue outbreak and identifying related climate factors contributing to the increase in the number of mosquitoes. Simulation model can be employed for this purpose. In this study, we created a simulation of system dynamic to predict the spread of dengue outbreak in Hulu Langat, Selangor Malaysia. The prototype was developed using STELLA 9.1.2 software. The main data input are rainfall, temperature and denggue cases. Data analysis from the graph showed that denggue cases can be predicted accurately using these two main variables- rainfall and temperature. However, the model will be further tested over a longer time period to ensure its accuracy, reliability and efficiency as a prediction tool for dengue outbreak.

Modelling the Role of Prophylaxis in Malaria Prevention

Year: 2008 Volume: 2 Issue: 3 94 - 99 Pages

Authors:
Farai Nyabadza

Abstract: Malaria is by far the world-s most persistent tropical parasitic disease and is endemic to tropical areas where the climatic and weather conditions allow continuous breeding of the mosquitoes that spread malaria. A mathematical model for the transmission of malaria with prophylaxis prevention is analyzed. The stability analysis of the equilibria is presented with the aim of finding threshold conditions under which malaria clears or persists in the human population. Our results suggest that eradication of mosquitoes and prophylaxis prevention can significantly reduce the malaria burden on the human population.

Effect of the Seasonal Variation in the Extrinsic Incubation Period on the Long Term Behavior of the Dengue Hemorrhagic Fever Epidemic

Year: 2007 Volume: 1 Issue: 10 478 - 484 Pages

Abstract: The incidences of dengue hemorrhagic disease (DHF) over the long term exhibit a seasonal behavior. It has been hypothesized that these behaviors are due to the seasonal climate changes which in turn induce a seasonal variation in the incubation period of the virus while it is developing the mosquito. The standard dynamic analysis is applied for analysis the Susceptible-Exposed- Infectious-Recovered (SEIR) model which includes an annual variation in the length of the extrinsic incubation period (EIP). The presence of both asymptomatic and symptomatic infections is allowed in the present model. We found that dynamic behavior of the endemic state changes as the influence of the seasonal variation of the EIP becomes stronger. As the influence is further increased, the trajectory exhibits sustained oscillations when it leaves the chaotic region.

Simulation of Population Dynamics of Aedes aegypti using Climate Dependent Model

Year: 2012 Volume: 6 Issue: 2 136 - 141 Pages

Abstract: A climate dependent model is proposed to simulate the population of Aedes aegypti mosquito. In developing the model, average temperature of Shah Alam, Malaysia was used to determine the development rate of each stage of the life cycle of mosquito. Rainfall dependent function was proposed to simulate the hatching rate of the eggs under several assumptions. The proposed transition matrix was obtained and used to simulate the population of eggs, larvae, pupae and adults mosquito. It was found that the peak of mosquito abundance comes during a relatively dry period following a heavy rainfall. In addition, lag time between the peaks of mosquito abundance and dengue fever cases in Shah Alam was estimated.

Mathematical Model of Dengue Disease with the Incubation Period of Virus

Year: 2008 Volume: 2 Issue: 8 530 - 534 Pages

Authors:
P. Pongsumpun

Abstract: Dengue virus is transmitted from person to person through the biting of infected Aedes Aegypti mosquitoes. DEN-1, DEN-2, DEN-3 and DEN-4 are four serotypes of this virus. Infection with one of these four serotypes apparently produces permanent immunity to it, but only temporary cross immunity to the others. The length of time during incubation of dengue virus in human and mosquito are considered in this study. The dengue patients are classified into infected and infectious classes. The infectious human can transmit dengue virus to susceptible mosquitoes but infected human can not. The transmission model of this disease is formulated. The human population is divided into susceptible, infected, infectious and recovered classes. The mosquito population is separated into susceptible, infected and infectious classes. Only infectious mosquitoes can transmit dengue virus to the susceptible human. We analyze this model by using dynamical analysis method. The threshold condition is discussed to reduce the outbreak of this disease.

Mosquitoes Larval Breeding Habitat in Urban and Suburban Areas, Peninsular Malaysia

Year: 2011 Volume: 5 Issue: 10 578 - 582 Pages

Abstract: Larval survey was carried out in 6 localities in the urban areas (Putrajaya) and suburban areas (Kuala Selangor) from January until December 2010. A total of 520 representative households in 6 localities were selected. Breeding habitats were sampled outdoors in the surroundings of housing areas. The study indicated that the most predominant species found in both areas was Aedes albopictus with the gardening utensil as a preferred breeding microhabitat for Putrajaya, in contrast to the artificial containers for Kuala Selangor. From a total of 1083 mosquito larvae species, 984 were Aedes albopictus larvae, 67 positive larvae of Aedes aegypti and 32 of Culex larvae. Aedes Index and Container Index were elevated in Putrajaya with 13% and 11% respectively which is higher than the standard given by the Ministry of Health, Malaysia. This results implicating dengue-sensitive skewed to the urban areas. Breteau Index result also above the standard in both study locations.

Top Journal

SUGGEST A JOURNAL