Abstract: An experiment was conducted under controlled
conditions to study the effect of water extract of leaves, shoots and
roots of either Sisymbrium irio L. =SISIR and or Descurainia
sophia (L.) Schur =DESSO on the germination and primary growth
of barley. A split-split plot experiment in CRD with three
replications was used. The main plots were the type of weed: i.e.
SISIR and DESSO and the sub-plots were type of organ: i.e. leaf,
stem and root and, the sub-sub plots were concentration of the
water extract of each organ of the weeds: i.e. 0, 2, 4 and 8 % w/v.
The results showed that the SISIR water extracts had a greater
inhibitory effects on the germination and primary growth of barley
than those of DESSO water extracts. The water extracts of the
leaves of both weeds had the greatest inhibitory effects on the
germination and primary growth of barley, compared to those of
stems and roots. Increasing the concentration of water extracts of
leaves, stems and roots of both weeds up to 8 % caused the greatest
inhibitory effects to barley and reduced the germination rate and
primary growth of it linearly.
Abstract: An experimental study is presented on the effect of
Conservation Agriculture (CA) compared to Conventional
Agriculture (ConvA) upon Maize Yield based on split-plot model.
Two factors have been considered: A Factor-Fertilization with two
variants: A1- N40P40 kg/ha and A2- N90P70 kg/ha; B Factor- Crop
protection with 4 variants : B1- 4 treatments, B2-3 treatments, B3- 2
treatments and B4- 1 treatment. In comparison with conventional
agriculture, CA determined lower maize yields. Fertilization is the
key factor determining a yield gain of 973.58 kg/ha in ConvA and
1,123.33 kg/ha in CA. A reduced number of treatments determined a
yield decline. The A-B interaction had a positive effect on maize
yield when a larger amount of fertilizer and 4 or 3 treatments were
applied in ConvA and a benefic in CA for highest fertilization level
and 2 treatments. The B2A2 ConvA variant was the most efficient
leading to 302.67 kg/ha gain while B3A2 CA variant brought 181.33
kg production gain.
Abstract: Gaharu that produced by Aquilaria spp. is classified as
one of the most valuable forest products traded internationally as it is
very resinous, fragrant and highly valuable heartwood. Gaharu has
been widely used in aromatheraphy, medicine, perfume and religious
practices. This work aimed to determine the factors affecting solid
liquid extraction of gaharu oil using hexane as solvent under
experimental condition. The kinetics of extraction was assumed and
verified based on a second-order mechanism. The effect of three
main factors, which were temperature, reaction time and solvent to
solid ratio were investigated to achieve maximum oil yield. The
optimum condition were found at temperature 65°C, 9 hours reaction
time and solvent to solid ratio of 12:1 with 14.5% oil yield. The
kinetics experimental data agrees and well fitted with the second
order extraction model. The initial extraction rate (h) was 0.0115
gmL-1min-1; the extraction capacity (Cs) was 1.282gmL-1; the second
order extraction constant (k) was 0.007 mLg-1min-1 and coefficient of
determination, R2 was 0.945.
Abstract: Dehydration process was carried out for tomato slices of var. Avinash after giving different pre-treatments such as calcium chloride (CaCl2), potassium metabisulphite (KMS), calcium chloride and potassium metabisulphite (CaCl2 +KMS), and sodium chloride (NaCl). Untreated samples served as control. Solar drier and continuous conveyor (tunnel) drier were used for dehydration. Quality characteristics of tomato slices viz. moisture content, sugar, titratable acidity, lycopene content, dehydration ratio, rehydration ratio and non-enzymatic browning as affected by dehydration process were studied. Storage study was also carried out for a period of six months for tomato powder packed into different types of packaging materials viz. metalized polyester (MP) film and low density poly ethylene (LDPE). Changes in lycopene content and non-enzymatic browning (NEB) were estimated during storage at room temperature. Pretreatment of 5 mm thickness of tomato slices with calcium chloride in combination with potassium metabisulphite and drying using a tunnel drier with subsequent storage of product in metalized polyester bags was selected as the best process.
Abstract: During 2011 and 2012 seasons, Zaghloul date palms received four sprays of silicon (Si) at 0.05 to 0.1% and selenium (Se) at 0.01 to 0.02%. Growth, nutritional status, yield as well as physical and chemical characteristics of the fruits in response to application of silicon and selenium were investigated. Single and combined applications of silicon at 0.05 to 0.1% and selenium at 0.01 to 0.02% was very effective in enhancing the leaf area, total chlorophylls, percentages of N, P and K in the leaves, yield, bunch weight as well as physical and chemical characteristics of the fruits in relative to the check treatment. Silicon was superior to selenium in this respect. Combined application was favorable than using each alone in this connection. Treating Zaghloul date palms four times with a mixture of silicon at 0.05% + selenium at 0.01% resulted in an economical yield and producing better fruit quality.
Abstract: The use of sewage sludge and effluents from
wastewater treatment plants for irrigation of agricultural lands is on
the rise particularly in peri-urban areas of developing countries.
The reuse of nutrients and organic matter in treated wastewater
and sewage sludge via land application is a desirable goal. However,
trace or heavy metals present in sludge pose the risk of human or
phytotoxicity from land application. Long-term use of sewage
sludge, heavy metals can accumulate to phytotoxic levels and results
in reduced plants growth and/or enhanced metal concentrations in
plants, which consumed by animals then enter the food chain. In this
research, the amount of heavy metals was measured in plants
irrigated with wastewater and sludge application. For this purpose,
three pilots were made in a Shush treatment plant in south of Tehran.
Three plants species, spinach, lettuce and radish were selected and
planted in the pilots.First pilot was irrigated just with wastewater of
treatment plant and second pilot was irrigated with wastewater and
sludge application .Third pilot was irrigated with simulated heavy
metals solution equal 50 years of irrigation. The results indicate that
the average of amount of heavy metals Pb, Cd in three plant species
in first pilot were lower than permissible limits .In second pilot,
Cadmium accumulations are high in three species plants and more
than the standard limits. Concentration of Cd , Pb have exceed their
permitted limits in plants in third pilot . It was concluded that the use
of wastewater and sludge application in agricultural lands enriched
soils with heavy metals to concentrations that may pose potential
environmental and health risks in the long-term.
Abstract: Present study summarizes the control of Vibrio
alginolyticus infection in hatchery reared Clownfish, Amphiprion
sebae with the extract of the mangrove plant, Avicennia marina.
Fishes with visible symptoms of hemorrhagic spots were chosen and
the genomic DNA of the causative bacterium was isolated and
sequenced based on 16S rDNA gene. The in vitro assay revealed that
a fraction of A. marina leaf extract elucidated with ethyl acetate:
methanol (6:4) showed a high activity (28 mm) at 125 μg/ml
concentrations. About 4 % of the fraction fed along with live V.
alginolyticus was significantly decreased the cumulative mortality
(P
Abstract: There is a world-wide need for the development of sustainable management strategies to control pest infestation and the development of phosphine (PH3) resistance in lesser grain borer (Rhyzopertha dominica). Computer simulation models can provide a relatively fast, safe and inexpensive way to weigh the merits of various management options. However, the usefulness of simulation models relies on the accurate estimation of important model parameters, such as mortality. Concentration and time of exposure are both important in determining mortality in response to a toxic agent. Recent research indicated the existence of two resistance phenotypes in R. dominica in Australia, weak and strong, and revealed that the presence of resistance alleles at two loci confers strong resistance, thus motivating the construction of a two-locus model of resistance. Experimental data sets on purified pest strains, each corresponding to a single genotype of our two-locus model, were also available. Hence it became possible to explicitly include mortalities of the different genotypes in the model. In this paper we described how we used two generalized linear models (GLM), probit and logistic models, to fit the available experimental data sets. We used a direct algebraic approach generalized inverse matrix technique, rather than the traditional maximum likelihood estimation, to estimate the model parameters. The results show that both probit and logistic models fit the data sets well but the former is much better in terms of small least squares (numerical) errors. Meanwhile, the generalized inverse matrix technique achieved similar accuracy results to those from the maximum likelihood estimation, but is less time consuming and computationally demanding.
Abstract: Sowing date and density are two important factors in
produce of coriander. A field experiment was conducted with
treatments: sowing time (5 May, 20 May, 4 June and 19 June 2009)
and plant density (10, 30, 50 and 70 plants m-2). The experimental
plots were laid out in a factorial according to a RCBD with three
replications. Results showed that the effect of sowing dates and
densities were significant on grain yield and yield components, but
interaction effects between sowing time and density were non
significant for all of traits in this trial. At sowing times 5 May, 20
May, 4 June and 19 June, grain yield obtained 736.9, 837.8, 1003.1
and 1299.6 kg ha-1, respectively. At 10, 30, 50 and 70 plants m-2,
grain yield were 794.9, 1031.0, 1092.3 and 959.3 kg ha-1,
respectively. In this experiment, sowing at 19 June and 50 and 30
plants m-2 had the most grain yield.
Abstract: Microbial contamination, most of which are fecal born in drinking water and food industry is a serious threat to humans. Escherichia coli is one of the most common and prevalent among them. We have developed a sensor for rapid and an early detection of contaminants, taking E.coli as a threat indicator organism. The sensor is based on co-polymerizations of aniline and formaldehyde in form of thin film over glass surface using the vacuum deposition technique. The particular doping combination of thin film with Fe-Al and Fe-Cu in different concentrations changes its non conducting properties to p- type semi conductor. This property is exploited to detect the different contaminants, believed to have the different surface charge. It was found through experiments that different microbes at same OD (0.600 at 600 nm) have different conductivity in solution. Also the doping concentration is found to be specific for attracting microbes on the basis of surface charge. This is a simple, cost effective and quick detection method which not only decreases the measurement time but also gives early warnings for highly contaminated samples.
Abstract: 15 strains of oil-destructing microorganisms were
isolated from oil polluted soil of Western Kazakhstan. Strains 2-A
and 41-3 with the highest oil-destructing activities were chosen from
them. It was shown that these strains oxidized n-alkanes very well,
but isoalkanes, isoparaffin, cycloparaffin and heavy aromatic
compounds were destructed very slowly. These both strains were
tested as preparations for bioremediation of oil-polluted soil in model
and field experiments. The degree of utilizing of soil oil by this
preparation was 79-84 % in field experiments.
Abstract: Plant tissue culture is an important in vitro technology applied for agricultural and industrial production. A sterile condition of culture medium is one of the main aspects. The alternative technique for medium sterilization to replace autoclaving was carried out. For sterilization of plant tissue culture medium without autoclaving, ten commercial pure essential oils and 5 disinfectants were tested. Each essential oil or disinfectant was added to a 20-mL Murashige and Skoog (MS) medium before medium was solidified in a 120-mL container, kept for 2 weeks before evaluating sterile conditions. Treated media, supplemented with essential oils or disinfectants, were compared to control medium, autoclaved at 121 degree Celsius for 15 min. Sterile conditions of MS medium were found 100% from betel oil or clove oil (18 mL/20 mL medium), cinnamon oil (36 mL/20 mL medium), lavender oil or holy basil oil (108 mL/20 mL medium), and lemon oil or tea tree oil or turmeric oil (252 mL/20 mL medium), compared to 100% sterile condition from autoclaved medium. For disinfectants, 2% iodine + 2.4% potassium iodide, 2% merbromine solution, 10% povidone-iodine, 6% sodium hypochlorite or 0.1% thimerosal at 36 mL/20 mL medium provided 100% sterile conditions. Furthermore, growth of new shoots from chrysanthemum node explants on treated media (fresh weight, shoot length, root length and number of node) were also reported and discussed in the comparison of those on autoclaved medium.
Abstract: This paper presents the new results of energy plant –
rye and triticale at yellow ripeness and ripe, pre-treatment in high
pressure steam reactor and monosaccharide extraction. There were
investigated the influence of steam pressure (20 to 22 bar), retention
duration (180 to 240 s) and catalytic sulphuric acid concentration
strength (0 to 0.5 %) on the pre-treatment process, contents of
monosaccharides (glucose, arabinose, xylose, mannose) and
undesirable by-compounds (furfural and HMF) in the reactor. The
study has determined that the largest amount of monosaccharides
(37.2 % of glucose, 2.7 % of arabinose, 8.4 % of xylose, and 1.3 %
of mannose) was received in the rye at ripe, the samples of which
were mixed with 0.5 % concentration of catalytic sulphuric acid, and
hydrolysed in the reactor, where the pressure was 20 bar, whereas the
reaction time – 240 s.
Abstract: The microbial production of ethanol from biodiesel¬derived crude glycerol by Enterobacter aerogenes TISTR1468, under micro-aerobic and anaerobic conditions, was investigated. The experimental results showed that micro-aerobic conditions were more favorable for cellular growth (4.0 g/L DCW), ethanol production (20.7 g/L) as well as the ethanol yield (0.47 g/g glycerol) than anaerobic conditions (1.2 g/L DCW, 6.3 g/L ethanol and 0.72 g/g glycerol, respectively). Crude glycerol (100 g/L) was consumed completely with the rate of 1.80 g/L/h. Two-stage fermentation (combination of micro-aerobic and anaerobic condition) exhibited higher ethanol production (24.5 g/L) than using one-stage fermentation (either micro-aerobic or anaerobic condition. The two- stage configuration, exhibited slightly higher crude glycerol consumption rate (1.81 g/L/h), as well as ethanol yield (0.56 g/g) than the one-stage configuration. Therefore, two-stage process was selected for ethanol production from E. aerogenes TISTR1468 in scale-up studies.
Abstract: The aim of a biological model is to understand the
integrated structure and behavior of complex biological systems as a
function of the underlying molecular networks to achieve simulation
and forecast of their operation. Although several approaches have
been introduced to take into account structural and environment
related features, relatively little attention has been given to represent
the behavior of biological systems. The Abstract Biological Process
(ABP) model illustrated in this paper is an object-oriented model
based on UML (the standard object-oriented language). Its main
objective is to bring into focus the functional aspects of the
biological system under analysis.
Abstract: The usefulness of weaning foods to meet the nutrient
needs of children is well recognized, and most of them are precooked
roller dried mixtures of cereal and/or legume flours which posses a
high viscosity and bulk when reconstituted. The objective of this study
was to formulate composite weaning foods using cereals, malted
legumes and vegetable powders and analyze them for nutrients,
functional properties and sensory attributes. Selected legumes (green
gram and lentil) were germinated, dried and dehulled. Roasted wheat,
rice, carrot powder and skim milk powder also were used. All the
ingredients were mixed in different proportions to get four
formulations, made into 30% slurry and dried in roller drier. The
products were analyzed for proximate principles, mineral content,
functional and sensory qualities. The results of analysis showed
following range of constituents per 100g of formulations on dry
weight basis, protein, 18.1-18.9 g ; fat, 0.78-1.36 g ; iron, 5.09-6.53
mg; calcium, 265-310 mg. The lowest water absorption capacity was
in case of wheat green gram based and the highest was in rice lentil
based sample. Overall sensory qualities of all foods were graded as
“good" and “very good" with no significant differences. The results
confirm that formulated weaning foods were nutritionally superior,
functionally appropriate and organoleptically acceptable.
Abstract: This research is to study the performance of heat
pump dryer for drying of kaffir lime leaves under different media and
to compare the color values and essential oil content of final products
after drying. In the experiments, kaffir lime leaves were dried in the
closed-loop system at drying temperatures of 40, 50 and 60 oC. The
drying media used in this study were hot air, CO2 and N2 gases. The
velocity of drying media in the drying chamber was 0.4 m/s with
bypass ratio of 30%. The initial moisture content of kaffir lime leaves
was approximately 180-190 % d.b. It was dried until down to a final
moisture content of 10% d.b. From the experiments, the results
showed that drying rate, the coefficient of performance (COP) and
specific energy consumption (SEC) depended on drying temperature.
While drying media did not affect on drying rate. The time for kaffir
lime leaves drying at 40, 50 and 60 oC was 10, 5 and 3 hours,
respectively. The performance of the heat pump system decreased
with drying temperature in the range of 2.20-3.51. In the aspect of
final product color, the greenness and overall color had a great
change under drying temperature at 60 oC rather than drying at 40
and 50 oC. When compared among drying media, the greenness and
overall color of product dried with hot air at 60 oC had a great change
rather than dried with CO2 and N2.
Abstract: The whole-cell protein-profiling technique was
evaluated for studying differences in banding pattern of three
different species of Cyanobacteria i.e. Anabaena fertilissima,
Aulosira fertilissima and Westiellopsis prolifica under the influence
of four different pesticides-2,4-D (Ethyl Ester of 2,4-Dichloro
Phenoxy Acetic Acid), Pencycuron (N-[(4-chlorophenyl)methyl]-Ncyclopentyl-
N'–phenylurea), Endosulfan (6,7,8,9,10,10hexachloro-
1,5,5a,6,9,9a-hexahydro-6,9-methano-2,4,3-benzodioxathiepine-3-
oxide) and Tebuconazole (1-(4-Chlorophenyl)-4,4-dimethyl-3-(1,2,4-
triazol-1-ylmethyl)pentan-3-ol). Whole-cell extracts were obtained by
sonication treatment (Sonifier cell disruptor -Branson Digital Sonifier
S-450D, USA) and were analyzed by sodium dodecyl sulfate
polyacrylamide gel electrophoresis (SDS-PAGE). SDS-PAGE
analyses of the total protein profile of Anabaena fertilissima,
Aulosira fertilissima and Westiellopsis prolifica showed a linear
decrease in the protein content with increasing pesticide stress when
administered to different concentrations of 2, 4-D, Pencycuron,
Endosulfan and Tebuconazole. The results indicate that different
stressors exert specific effects on cyanobacterial protein synthesis.
Abstract: P16/INK4A is tumor suppressor protein that plays a critical role in cell cycle regulation. Loss of P16 protein expression has been implicated in pathogenesis of many cancers, including lymphoma. Therefore, we sought to investigate if loss of P16 protein expression is associated with lymphoma and/or any specific lymphoma subtypes (Hodgkin-s lymphoma (HL) and nonHodgkin-s lymphoma (NHL)). Fifty-five lymphoma cases consisted of 30 cases of HL and 25 cases of NHL, with an age range of 3 to 78 years, were examined for loss of P16 by immunohistochemical technique using a specific antibody reacting against P16. In total, P16 loss was seen in 33% of all lymphoma cases. P16 loss was identified in 47.7% of HL cases. In contrast, only 16% of NHL showed loss of P16. Loss of P16 was seen in 67% of HL patients with 50 years of age or older, whereas P16 loss was found in only 42% of HL patients with less than 50 years of age. P16 loss in HL is somewhat higher in male (55%) than in female (30%). In subtypes of HL, P16 loss was found exclusively in all cases of lymphocyte depletion, lymphocyte predominance and unclassified cases, whereas P16 loss was seen in 39% of mixed cellularity and 29% of nodular sclerosis cases. In low grade NHL patients, P16 loss was seen in approximately one-third of cases, whereas no or very rare of P16 loss was found in intermediate and high grade cases. P16 loss did not show any correlation with age or gender of NHL patients. In conclusion, the high rate of P16 loss seen in our study suggests that loss of P16 expression plays a critical role in the pathogenesis of lymphoma, particularly with HL.
Abstract: Yogurt is a coagulated milk product obtained from
the lactic acid fermentation by the action of Lactobacillus
bulgaricus and Streptococcus thermophilus. The additions of fruits
into milk may enhance the taste and the therapeutical values of milk
products. However fruits also may change the fermentation
behaviour. In this present study, the changes in physicochemical, the
peptide concentration, total phenolics content and the antioxidant
potential of yogurt upon the addition of Hylocereus polyrhizus and
Hylocereus undatus (white and red dragon fruit) were investigated.
Fruits enriched yogurt (10%, 20%, 30% w/w) were prepared and the
pH, TTA, syneresis measurement, peptide concentration, total
phenolics content and DPPH antioxidant inhibition percentage were
determined. Milk fermentation rate was enhanced in red dragon fruit
yogurt for all doses (-0.3606 - -0.4126 pH/h) while only white
dragon fruit yogurt with 20% and 30% (w/w) composition showed
increment in fermentation rate (-0.3471 - -0.3609 pH/h) compared to
plain yogurt (-0.3369pH/h). All dragon fruit enriched yogurts
generally showed lower pH readings (pH 3.95 - 4.03) compared to
plain yogurt (pH 4.05). Both fruit yogurts showed a higher lactic
acid percentage (1.14-1.23%) compared to plain yogurt (1.08%).
Significantly higher syneresis percentage (57.19 - 70.32%)
compared to plain yogurt (52.93%) were seen in all fruit enriched
yogurts. The antioxidant activity of plain yogurt (19.16%) was
enhanced by the presence of white and red dragon fruit (24.97-
45.74%). All fruit enriched yogurt showed an increment in total
phenolic content (36.44 - 64.43mg/ml) compared to plain yogurt
(20.25mg/ml). However, the addition of white and red dragon fruit
did not enhance the proteolysis of milk during fermentation.
Therefore, it could be concluded that the addition of white and red
dragon fruit into yogurt enhanced the milk fermentation rate, lactic
acid content, syneresis percentage, antioxidant activity, and total
phenolics content in yogurt.