An Evaluation of Pesticide Stress Induced Proteins in three Cyanobacterial Species-Anabaena Fertilissima, Aulosira Fertilissima and Westiellopsis Prolifica using SDS-PAGE
The whole-cell protein-profiling technique was
evaluated for studying differences in banding pattern of three
different species of Cyanobacteria i.e. Anabaena fertilissima,
Aulosira fertilissima and Westiellopsis prolifica under the influence
of four different pesticides-2,4-D (Ethyl Ester of 2,4-Dichloro
Phenoxy Acetic Acid), Pencycuron (N-[(4-chlorophenyl)methyl]-Ncyclopentyl-
N'–phenylurea), Endosulfan (6,7,8,9,10,10hexachloro-
1,5,5a,6,9,9a-hexahydro-6,9-methano-2,4,3-benzodioxathiepine-3-
oxide) and Tebuconazole (1-(4-Chlorophenyl)-4,4-dimethyl-3-(1,2,4-
triazol-1-ylmethyl)pentan-3-ol). Whole-cell extracts were obtained by
sonication treatment (Sonifier cell disruptor -Branson Digital Sonifier
S-450D, USA) and were analyzed by sodium dodecyl sulfate
polyacrylamide gel electrophoresis (SDS-PAGE). SDS-PAGE
analyses of the total protein profile of Anabaena fertilissima,
Aulosira fertilissima and Westiellopsis prolifica showed a linear
decrease in the protein content with increasing pesticide stress when
administered to different concentrations of 2, 4-D, Pencycuron,
Endosulfan and Tebuconazole. The results indicate that different
stressors exert specific effects on cyanobacterial protein synthesis.
[1] O. A. Herrero, A. M. Muro-Pastor and E. Flores, "Nitrogen Controlling
Cyanobacteria," J. Bacteriol., vol. 183, pp. 411-425, 2001.
[2] U. Mishra and S. Pabby, "Cyanobacteria: A Potential Biofertilizer for
Rice," Res., 6-10, 2004.
[3] F. B. Pankratz, C. Doebel, A. Farenhorst and L. G. Goldsborough,
"Interactions between Algae (Selenastrum Capricornutum) and
Pesticides: Implications for Managing Constructed Wetlands for
Pesticides Removal," J. Env. Sci. Health: B., vol. 38, pp. 147-155, 2003.
[4] T. Krech, J. De Chastonay and E. Falsen, "Epidemiology of diptheria:
polypeptide and restriction enzyme analysis in comparison with
conventional phage typing," Eur. J. Cli. Microbiol. Infect. Dis., vol. 7,
pp. 232-237, 1988.
[5] M. T. Giardi, J. Masojidek and D. Godde, "Effects of Abiotic Stresses
on the Turnover of the D1reaction Center II Protein," Plant Physiol., vol.
101, pp. 635-642, 1997.
[6] L. Stal, "Cyanobacteria: Diversity and versatility. Clues to life in
Extreme Environments," in Algae and cyanobacteria in extreme
environments, 2007, pp. 659-680.
[7] J. Rath and S. P. Adhikary, "Response of the Estuarine Cyanobacterium
Lyngbya Aestuarii to UV-B Radiation," J. App. Phycol., vol. 19, pp.
529-536, 2007.
[8] J. I. Nirmal Kumar, B. Anubhuti and A. Manmeet Kaur, "Chronic
Toxicity of a Triazole Fungicide-Tebuconazole on the Growth and
Metabolic Activities of Heterocystous, Nitrogen-Fixing Paddy Field
Cyanobacterium, Westiellopsis prolifica, Janet," J. Microbiol. Biotech.,
vol. 20(7), pp. 1134-1139, 2010.
[9] P. Bhargava, M. Yogesh, S. Ashish Kumar, A. Anjum and R. Lal Chand,
"Preliminary Analysis of Cuprome of Anabaena Doliolum using Two-
Dimensional Gel Electrophoresis," Curr. Sci., vol. 91, pp. 1521-152,
2006.
[10] R. P. Sinha, N. Singh, A. Kumar, H. D. Kumar, M. Häder and D. P.
Häder, "Effects of UV Irradiation on Certain Physiological and
Biochemical Processes in Cyanobacteria," J. Photochem. Photobiol. B:
Biology, vol. 32, pp. 107-113, 1996.
[11] S. Zutshi, Jamia Millia Islamia University, New Delhi, India., Heavy
metal stress induced enzymes in cyanobacteria, Ph.D thesis, 2009.
[12] J. I. Nirmal Kumar, A. Manmeet Kaur and B. Anubhuti, "Differential
Effect of Phenoxy Substituted Herbicide 2, 4-D on Pigments,
Metabolites and Enzyme Activities of three Species of Cyanobacteria,"
Int. J. Biosci. Technol., vol. 2, pp. 87-95, 2009.
[13] R. Rippka, S. Deruelles, J. B. Waterbury, M. Herdman and R. Y.
Stanier, "Generic Assignments, Strain Histories and Properties of Pure
Cultures of Cyanobacteria," J. Gen. Microbiol., vol. 111, pp. 1-61, 1979.
[14] F. Gentilli, M. C. Nilsson, O. Zackrisson, T. H. DeLuca and A. Sellstedt,
"Physiological and Molecular Diversity of Feather Moss Associative
N2-Fixing Cyanobacteria," J. Exp. Bot., vol. 56, pp. 3121-3127, 2005.
[15] U. K. Laemmli, "Cleavage of Structural Proteins during the Assembly of
the Head of Bacteriophage T4," Nature, vol. 227, pp. 680-685, 1970.
[16] A. Weber and K. Jung, "Profiling Early Osmostress-Dependent Gene
Expression in Escherichia Coli Using DNA Macroarrays," J. Bacteriol.,
vol. 184, pp. 5502-5507, 2002.
[17] U. M. Rajendran, K. Elango and N. Anand, "Effects of a Fungicide, an
Insecticide and a Biopesticide on Tolypothrix scytonemoides," Pest.
Biochem. Physiol., vol. 87, pp. 164-171, 2007.
[18] K. Freemark, "The Use of Plants for Environmental Monitoring and
Assessment," Ecotoxicol. Env. Saf., vol. 30, pp. 289 - 301, 1995.
[19] S. K. Saha, L. Uma and S. Gopalakrishnan, "Nitrogen Stress Induced
Changes in the Marine Cyanobacterium Oscillatoria willei BDU
130511," FEMS Microbiol. Eco., vol. 45, pp. 263-272, 2003.
[20] E. A. Fern├índez-Valiente, U. A. Quesada, F. Leganés and R. Careres,
Contribution of N Fixing Cyanobacteria to Rice Production: Availability
of Nitrogen from N-Labelled Cyanobacteria and Ammonium Sulphate to
Rice. Plant Soil, vol. 211, pp. 107-112, 2000.
[1] O. A. Herrero, A. M. Muro-Pastor and E. Flores, "Nitrogen Controlling
Cyanobacteria," J. Bacteriol., vol. 183, pp. 411-425, 2001.
[2] U. Mishra and S. Pabby, "Cyanobacteria: A Potential Biofertilizer for
Rice," Res., 6-10, 2004.
[3] F. B. Pankratz, C. Doebel, A. Farenhorst and L. G. Goldsborough,
"Interactions between Algae (Selenastrum Capricornutum) and
Pesticides: Implications for Managing Constructed Wetlands for
Pesticides Removal," J. Env. Sci. Health: B., vol. 38, pp. 147-155, 2003.
[4] T. Krech, J. De Chastonay and E. Falsen, "Epidemiology of diptheria:
polypeptide and restriction enzyme analysis in comparison with
conventional phage typing," Eur. J. Cli. Microbiol. Infect. Dis., vol. 7,
pp. 232-237, 1988.
[5] M. T. Giardi, J. Masojidek and D. Godde, "Effects of Abiotic Stresses
on the Turnover of the D1reaction Center II Protein," Plant Physiol., vol.
101, pp. 635-642, 1997.
[6] L. Stal, "Cyanobacteria: Diversity and versatility. Clues to life in
Extreme Environments," in Algae and cyanobacteria in extreme
environments, 2007, pp. 659-680.
[7] J. Rath and S. P. Adhikary, "Response of the Estuarine Cyanobacterium
Lyngbya Aestuarii to UV-B Radiation," J. App. Phycol., vol. 19, pp.
529-536, 2007.
[8] J. I. Nirmal Kumar, B. Anubhuti and A. Manmeet Kaur, "Chronic
Toxicity of a Triazole Fungicide-Tebuconazole on the Growth and
Metabolic Activities of Heterocystous, Nitrogen-Fixing Paddy Field
Cyanobacterium, Westiellopsis prolifica, Janet," J. Microbiol. Biotech.,
vol. 20(7), pp. 1134-1139, 2010.
[9] P. Bhargava, M. Yogesh, S. Ashish Kumar, A. Anjum and R. Lal Chand,
"Preliminary Analysis of Cuprome of Anabaena Doliolum using Two-
Dimensional Gel Electrophoresis," Curr. Sci., vol. 91, pp. 1521-152,
2006.
[10] R. P. Sinha, N. Singh, A. Kumar, H. D. Kumar, M. Häder and D. P.
Häder, "Effects of UV Irradiation on Certain Physiological and
Biochemical Processes in Cyanobacteria," J. Photochem. Photobiol. B:
Biology, vol. 32, pp. 107-113, 1996.
[11] S. Zutshi, Jamia Millia Islamia University, New Delhi, India., Heavy
metal stress induced enzymes in cyanobacteria, Ph.D thesis, 2009.
[12] J. I. Nirmal Kumar, A. Manmeet Kaur and B. Anubhuti, "Differential
Effect of Phenoxy Substituted Herbicide 2, 4-D on Pigments,
Metabolites and Enzyme Activities of three Species of Cyanobacteria,"
Int. J. Biosci. Technol., vol. 2, pp. 87-95, 2009.
[13] R. Rippka, S. Deruelles, J. B. Waterbury, M. Herdman and R. Y.
Stanier, "Generic Assignments, Strain Histories and Properties of Pure
Cultures of Cyanobacteria," J. Gen. Microbiol., vol. 111, pp. 1-61, 1979.
[14] F. Gentilli, M. C. Nilsson, O. Zackrisson, T. H. DeLuca and A. Sellstedt,
"Physiological and Molecular Diversity of Feather Moss Associative
N2-Fixing Cyanobacteria," J. Exp. Bot., vol. 56, pp. 3121-3127, 2005.
[15] U. K. Laemmli, "Cleavage of Structural Proteins during the Assembly of
the Head of Bacteriophage T4," Nature, vol. 227, pp. 680-685, 1970.
[16] A. Weber and K. Jung, "Profiling Early Osmostress-Dependent Gene
Expression in Escherichia Coli Using DNA Macroarrays," J. Bacteriol.,
vol. 184, pp. 5502-5507, 2002.
[17] U. M. Rajendran, K. Elango and N. Anand, "Effects of a Fungicide, an
Insecticide and a Biopesticide on Tolypothrix scytonemoides," Pest.
Biochem. Physiol., vol. 87, pp. 164-171, 2007.
[18] K. Freemark, "The Use of Plants for Environmental Monitoring and
Assessment," Ecotoxicol. Env. Saf., vol. 30, pp. 289 - 301, 1995.
[19] S. K. Saha, L. Uma and S. Gopalakrishnan, "Nitrogen Stress Induced
Changes in the Marine Cyanobacterium Oscillatoria willei BDU
130511," FEMS Microbiol. Eco., vol. 45, pp. 263-272, 2003.
[20] E. A. Fern├índez-Valiente, U. A. Quesada, F. Leganés and R. Careres,
Contribution of N Fixing Cyanobacteria to Rice Production: Availability
of Nitrogen from N-Labelled Cyanobacteria and Ammonium Sulphate to
Rice. Plant Soil, vol. 211, pp. 107-112, 2000.
@article{"International Journal of Biological, Life and Agricultural Sciences:61281", author = "Nirmal Kumar and Rita N. Kumar and Anubhuti Bora and Manmeet Kaur Amb", title = "An Evaluation of Pesticide Stress Induced Proteins in three Cyanobacterial Species-Anabaena Fertilissima, Aulosira Fertilissima and Westiellopsis Prolifica using SDS-PAGE", abstract = "The whole-cell protein-profiling technique was
evaluated for studying differences in banding pattern of three
different species of Cyanobacteria i.e. Anabaena fertilissima,
Aulosira fertilissima and Westiellopsis prolifica under the influence
of four different pesticides-2,4-D (Ethyl Ester of 2,4-Dichloro
Phenoxy Acetic Acid), Pencycuron (N-[(4-chlorophenyl)methyl]-Ncyclopentyl-
N'–phenylurea), Endosulfan (6,7,8,9,10,10hexachloro-
1,5,5a,6,9,9a-hexahydro-6,9-methano-2,4,3-benzodioxathiepine-3-
oxide) and Tebuconazole (1-(4-Chlorophenyl)-4,4-dimethyl-3-(1,2,4-
triazol-1-ylmethyl)pentan-3-ol). Whole-cell extracts were obtained by
sonication treatment (Sonifier cell disruptor -Branson Digital Sonifier
S-450D, USA) and were analyzed by sodium dodecyl sulfate
polyacrylamide gel electrophoresis (SDS-PAGE). SDS-PAGE
analyses of the total protein profile of Anabaena fertilissima,
Aulosira fertilissima and Westiellopsis prolifica showed a linear
decrease in the protein content with increasing pesticide stress when
administered to different concentrations of 2, 4-D, Pencycuron,
Endosulfan and Tebuconazole. The results indicate that different
stressors exert specific effects on cyanobacterial protein synthesis.", keywords = "Cyanobacteria, pesticide, SDS-PAGE", volume = "5", number = "3", pages = "161-8", }
