Effect of Process Parameters on Aerobic Decolourization of Reactive Azo Dye using Mixed Culture
In the present study, an attempt was made to examine
the potential of aerobic mixed culture for decolourization of Remazol Black B dye in batch reactors. The effect of pH, temperature, inoculum, initial concentration of dye and initial concentration of
glucose was studied with an aim to determine the optimal conditions
required for maximum decolourization and degradation. The culture
exhibited maximum decolourization ability at pH between 7-8 and at
30°C. A 10% (v/v) inoculum and 1% (w/v) glucose concentration
were found to be the optimum for decolourization. A maximum of
98% decolourization was observed at 25 ppm initial concentration of
dye after 18 hours of incubation period. At higher dye concentration
of 300 ppm, the removal in colour was found to be 75% in 48 hours of incubation period. The results show that the enriched mixed culture from activated sludge has good potential in removal of Remazol Black B dye from wastewater under aerobic conditions.
[1] Banat, IM.; Nigam , P.; Singh, D.; Marchant, R. Microbial decolorization
of textile-dye containing effluents - a review. Bioresource Technology. 1996 (58) 217-227.
[2] Wang, W. Toxicity assessment of pretreated industrial effluent using higher
plant. Research Journal Water Pollution Control Federation. 1991, (62) 853-
860.
[3] Kapustka, L.A.; Reporter, M. Terrestrial primary producers, in: P. Calow
(Ed.), Handbook of Ecotoxicology, vol. 1, Blackwell Scientific Publications,
Oxford, 1993, 278-297.
[4] Kalyuzhnyi, S.; Sklyar, V. Biomineralisation of azo dyes and their
breakdown products in anaerobic-aerobic hybrid and UASB reactors. Water
Science Technology. 2000, ( 41) 23- 30.
[5] Kariminiaae, Hamedaani.; Sakurai, A.; Sakakibara, M.; Decolorization of
synthetic dyes by a new manganese peroxidase-producing white rot fungus.
Dyes Pigments. 2007 (72) 157-162.
[6] Asad, S.; Amoozegar,M.A,; Pourbabaee, A.A.; Sarbolouki, M.N.;
Dastgheib, S.M.M. Decolorization of textile azo dyes by newly isolated
halophilic and halotolerant bacteria. Bioresource Technology. 2007, 2082-
2088.
[7] Moosvi,S.;Kher,X.;Madamwar, D. Isolation, characterization and
decolorization of textile dyes by amixed bacterial consortium JW-2. Dyes
Pigments. 2007 (74) 723-729.
[8] Aksu, Z.; Tezer, S. Equilibrium and kinetic modelling of biosorption of
Remazol Black B by Rhizopus arrhizus in a batch system: effect of
Temperature. Process Biochemistry.2000 (36) 431-439.
[9] Kirby, N.; Marchant, R.; McMullan, G. Decolourisation of synthetic textile
dyes by Phlebia tremellosa. FEMS Microbiology Letters. 2000 (188) 93-96.
[10] Swamy, J.; Ramsay, J.A.The evaluation of white rot fungi in the
decolouration of textile dyes. Enz Microbial Technology.1999 (24) 130-137.
[11] Aksu, Z.; Donmez, G. Combined effects of molasses sucrose and reactive
dye on the growth and dye bioaccumulation properties of Candida tropicalis.
Process Biochemistry. 2005 (40) 2443-2454.
[12] Meehan, C.; Banat, IM.; McMullan, G.; Nigam, P.; Smyth, F.; Marchant, R.
Decolorization of Remazol Black-B using a thermotolerant yeast,
Kluyveromyces marxianus IMB3. Environment Internatioanl. 2000. (1-2) 75-
79.
[13] Robinson, T.; Chandran, B.; Nigam, P. Effect of pretreatments of three waste
resiudes, wheat straw, corncobs and barley husks on dye adsorption.
Bioresour Technol. 2002 (85) 119-124.
[14] Hepel,M.; Hazelton,S. Photoelectrochemical Degradation of Diazo Dyes on
Nanostructured Electrodes Electrochim Acta. 2005 (50) 5278-5291.
[15] Vinodgopal, K.; Peller, J.; Makogon, O.; Kamat, P.V. Ultrasonic
mineralization of a reactive textile azo dye, Remazol Black B. Water
Research. 1998(32) 3646.
[16] Pearce, CI.; Lloyd, JR.; Guthrie, JT. The removal of colour from textile
wastewater using whole bacterial cells: a review. Dyes and Pigment. 2003
(58),179-196.
[17] Andre, BDS.; Francisco, JC.; Jules, BVL. Review paper on current
technologies for decolourisation of textile wastewaters: perspectives for
anaerobic biotechnology. Bioresource Technology. 2007 (98) 2369- 2385.
[18] Chang, Jo-Shu.; Chen, Bor-Yann.; Lin, YS. Stimulation of bacterial
decolorization of an azo dye by extracellular metabolites from Escherichia
coli strain NO3. Bioresource Technology. 2004 (91) 243-48.
[19] Chen, K.C.; Huang, W.T.; Wu, J.Y.; Houng, J.Y. Microbial decolorization of
azo dyes by Proteus mirabilis, Journal of Industrial Microbiology and
Biotechnology. 1999 (23) 686-690.
[20] Aksu, Z. Reactive dye bioaccumulation by Saccharomyces cerevisiae.
Process Biochem. 2003(10) 1437-1444.
[21] Cetin, D.; Donmez,G. Decolorization of reactive dyes by mixed cultures
isolated from textile effluent under anaerobic conditions. Enzyme and
Microbial Technology. 2006 (38) 926-930.
[1] Banat, IM.; Nigam , P.; Singh, D.; Marchant, R. Microbial decolorization
of textile-dye containing effluents - a review. Bioresource Technology. 1996 (58) 217-227.
[2] Wang, W. Toxicity assessment of pretreated industrial effluent using higher
plant. Research Journal Water Pollution Control Federation. 1991, (62) 853-
860.
[3] Kapustka, L.A.; Reporter, M. Terrestrial primary producers, in: P. Calow
(Ed.), Handbook of Ecotoxicology, vol. 1, Blackwell Scientific Publications,
Oxford, 1993, 278-297.
[4] Kalyuzhnyi, S.; Sklyar, V. Biomineralisation of azo dyes and their
breakdown products in anaerobic-aerobic hybrid and UASB reactors. Water
Science Technology. 2000, ( 41) 23- 30.
[5] Kariminiaae, Hamedaani.; Sakurai, A.; Sakakibara, M.; Decolorization of
synthetic dyes by a new manganese peroxidase-producing white rot fungus.
Dyes Pigments. 2007 (72) 157-162.
[6] Asad, S.; Amoozegar,M.A,; Pourbabaee, A.A.; Sarbolouki, M.N.;
Dastgheib, S.M.M. Decolorization of textile azo dyes by newly isolated
halophilic and halotolerant bacteria. Bioresource Technology. 2007, 2082-
2088.
[7] Moosvi,S.;Kher,X.;Madamwar, D. Isolation, characterization and
decolorization of textile dyes by amixed bacterial consortium JW-2. Dyes
Pigments. 2007 (74) 723-729.
[8] Aksu, Z.; Tezer, S. Equilibrium and kinetic modelling of biosorption of
Remazol Black B by Rhizopus arrhizus in a batch system: effect of
Temperature. Process Biochemistry.2000 (36) 431-439.
[9] Kirby, N.; Marchant, R.; McMullan, G. Decolourisation of synthetic textile
dyes by Phlebia tremellosa. FEMS Microbiology Letters. 2000 (188) 93-96.
[10] Swamy, J.; Ramsay, J.A.The evaluation of white rot fungi in the
decolouration of textile dyes. Enz Microbial Technology.1999 (24) 130-137.
[11] Aksu, Z.; Donmez, G. Combined effects of molasses sucrose and reactive
dye on the growth and dye bioaccumulation properties of Candida tropicalis.
Process Biochemistry. 2005 (40) 2443-2454.
[12] Meehan, C.; Banat, IM.; McMullan, G.; Nigam, P.; Smyth, F.; Marchant, R.
Decolorization of Remazol Black-B using a thermotolerant yeast,
Kluyveromyces marxianus IMB3. Environment Internatioanl. 2000. (1-2) 75-
79.
[13] Robinson, T.; Chandran, B.; Nigam, P. Effect of pretreatments of three waste
resiudes, wheat straw, corncobs and barley husks on dye adsorption.
Bioresour Technol. 2002 (85) 119-124.
[14] Hepel,M.; Hazelton,S. Photoelectrochemical Degradation of Diazo Dyes on
Nanostructured Electrodes Electrochim Acta. 2005 (50) 5278-5291.
[15] Vinodgopal, K.; Peller, J.; Makogon, O.; Kamat, P.V. Ultrasonic
mineralization of a reactive textile azo dye, Remazol Black B. Water
Research. 1998(32) 3646.
[16] Pearce, CI.; Lloyd, JR.; Guthrie, JT. The removal of colour from textile
wastewater using whole bacterial cells: a review. Dyes and Pigment. 2003
(58),179-196.
[17] Andre, BDS.; Francisco, JC.; Jules, BVL. Review paper on current
technologies for decolourisation of textile wastewaters: perspectives for
anaerobic biotechnology. Bioresource Technology. 2007 (98) 2369- 2385.
[18] Chang, Jo-Shu.; Chen, Bor-Yann.; Lin, YS. Stimulation of bacterial
decolorization of an azo dye by extracellular metabolites from Escherichia
coli strain NO3. Bioresource Technology. 2004 (91) 243-48.
[19] Chen, K.C.; Huang, W.T.; Wu, J.Y.; Houng, J.Y. Microbial decolorization of
azo dyes by Proteus mirabilis, Journal of Industrial Microbiology and
Biotechnology. 1999 (23) 686-690.
[20] Aksu, Z. Reactive dye bioaccumulation by Saccharomyces cerevisiae.
Process Biochem. 2003(10) 1437-1444.
[21] Cetin, D.; Donmez,G. Decolorization of reactive dyes by mixed cultures
isolated from textile effluent under anaerobic conditions. Enzyme and
Microbial Technology. 2006 (38) 926-930.
@article{"International Journal of Biological, Life and Agricultural Sciences:61586", author = "Kapil Kumar and M. G. Dastidar and T. R. Sreekrishnan", title = "Effect of Process Parameters on Aerobic Decolourization of Reactive Azo Dye using Mixed Culture", abstract = "In the present study, an attempt was made to examine
the potential of aerobic mixed culture for decolourization of Remazol Black B dye in batch reactors. The effect of pH, temperature, inoculum, initial concentration of dye and initial concentration of
glucose was studied with an aim to determine the optimal conditions
required for maximum decolourization and degradation. The culture
exhibited maximum decolourization ability at pH between 7-8 and at
30°C. A 10% (v/v) inoculum and 1% (w/v) glucose concentration
were found to be the optimum for decolourization. A maximum of
98% decolourization was observed at 25 ppm initial concentration of
dye after 18 hours of incubation period. At higher dye concentration
of 300 ppm, the removal in colour was found to be 75% in 48 hours of incubation period. The results show that the enriched mixed culture from activated sludge has good potential in removal of Remazol Black B dye from wastewater under aerobic conditions.", keywords = "Aerobic conditions, Decolourization, Mixed culture, Remazol Black B.", volume = "3", number = "10", pages = "530-4", }
