Abstract: Sweet cherries (Prunus avium L.) contain various
phenolic compounds which contribute to total antioxidant activity.
Total polyphenols, tannins, flavonoids and anthocyanins, and
antioxidant capacity in a fruits of a number of selected sweet cherry
genotypes were investigated. Total polyphenols content ranged from
4.12 to 8.34 mg gallic acid equivantents/g dry fruit weight and total
tannins content ranged from 0.19 to 1.95 mg gallic acid equivalent/g
dry fruit weight. Total flavonoids were within the range 0.42-1.56 mg
of rutin equivalents/g dry fruit weight and total anthocyanins content
were between 0.35 and 0.69 mg cyanidin 3-glucoside equivalent/ g
dry fruit weight. Although sweet cherry fruits are a significant source
of different phenolic compounds, antioxidant activity of sweet
cherries is not related only with the total polyphenolics, flavonoids or
anthocyanins.
Abstract: In this research the level of mercury is analyzed in
muscle tissue of Otolithes ruber retailed in Hamedan, Iran were
determined by flame atomic absorption spectrometry after wet
digestion. Analysis of mercury was carried out by
spectrophotometrically. The average concentration of Hg in muscle
tissue of Otolithes ruber was 0.030±0.026 -g/g so lower than to
compare with the Maximum Allowable Concentration determined by
FAO/WHO Codex Alimentarius Commission.
Abstract: In this study Vitaton (an organic supplement which
contains fermentative β-carotene) and synthetic astaxanthin
(CAROPHYLL® Pink) were evaluated as pro-growth factors in
Rainbow trout diet. An 8 week feeding trial was conducted to
determine the effects of Vitaton versus astaxanthin on rearing factors,
survival rate and visual flesh color of Rainbow trout
(Oncorhnchynchus mykiss) with initial weight of 196±5. Four
practical diets were formulated to contain 50 and 80 (ppm) of β-
carotene and astaxanthin and also a control diet was prepared without
any pigment. Each diet was fed to triplicate groups of fish rearing in
fresh water. Fish were fed twice daily. The water temperature
fluctuated from 12 to 15 (C˚) and also dissolved oxygen content was
between 7 to 7.5 (mg/lit) during the experimental period. At the end
of the experiment, growth and food utilization parameters and
survival rate were unaffected by dietary treatments (p>0.05). Also,
there was no significant difference between carcass yield within
treatments (p>0.05). No significant difference recognized between
visual flesh color (SalmoFan score) of fish fed Vitaton-containing
diets. On the contrary, feeding on diets containing 50 and 80 (ppm)
of astaxanthin, increased SalmoFan score (flesh astaxanthin
concentration) from
Abstract: Rice seed expression (cDNA) library in the Lambda
Zap 11® phage constructed from the developing grain 10-20 days
after flowering was transformed into yeast for functional
complementation assays in three salt sensitive yeast mutants S.
cerevisiae strain CY162, G19 and Axt3K. Transformed cells of G19
and Axt3K with pYES vector with cDNA inserts showed enhance
tolerance than those with empty pYes vector. Sequencing of the
cDNA inserts revealed that they encode for the putative proteins with
the sequence homologous to rice putative protein PROLM24
(Os06g31070), a prolamin precursor. Expression of this cDNA did
not affect yeast growth in absence of salt. Axt3k and G19 strains
expressing the PROLM24 were able to grow upto 400 mM and 600
mM of NaCl respectively. Similarly, Axt3k mutant with PROLM24
expression showed comparatively higher growth rate in the medium
with excess LiCl (50 mM). The observation that expression of
PROLM24 rescued the salt sensitive phenotypes of G19 and Axt3k
indicates the existence of a regulatory system that ameliorates the
effect of salt stress in the transformed yeast mutants. However, the
exact function of the cDNA sequence, which shows partial sequence
homology to yeast UTR1 is not clear. Although UTR1 involved in
ferrous uptake and iron homeostasis in yeast cells, there is no
evidence to prove its role in Na+ homeostasis in yeast cells. Absence
of transmembrane regions in Os06g31070 protein indicates that salt
tolerance is achieved not through the direct functional
complementation of the mutant genes but through an alternative
mechanism.
Abstract: The objective of the present study was to examine the
dose-response relationships between antioxidant parameters and liver
contaminant levels of Kazakhstan light crude oil (KLCO) in albino
rats. The animals were repeatedly exposed, by intraperitoneal
injection, to low dosages (0.5–1.5 ml/kg) of KLCO. Rats exposed to
these doses levels did not show any apparent symptoms of
intoxication. Serum aminotransferases increased significantly
(p
Abstract: In this paper we evaluated the efficacy of
photodynamic treatment of infected wounds on pig animal model by
diffuse reflectance spectrometry. The study was conducted on fifteen
wounds contaminated with Staphylococcus aureus bacteria that were
incubated for 30 min with methylene blue solution (c = 3.3 x 10-3 M)
and exposed to laser radiations (λ = 670 nm, P = 15 mW) for 15 min.
The efficiency of photodynamic inactivation of bacteria was
evaluated by microbiological exams and diffuse reflectance
spectrometry. The results of the microbiological exams showed that
the bacterial concentration has decreased from 6.93±0.138
logCFU/ml to 3.12±0.108 logCFU/ml. The spectral examination
showed that the diffuse reflectance of wounds contaminated with
Staphylococcus aureus has decreased from 5.06±0.036 % to
3.36±0.025 %. In conclusion, photodynamic therapy is an effective
method for the treatment of infected wounds and there is a correlation
between the CFU count and diffuse reflectance.
Abstract: The paper presents the influence of the conventional
ploughing tillage technology in comparison with the minimum
tillage, upon the soil properties, weed control and yield in the case of
maize (Zea mays L.), soya-bean (Glycine hispida L.) and winter
wheat (Triticum aestivum L.) in a three years crop rotation. A
research has been conducted at the University of Agricultural
Sciences and Veterinary Medicine Cluj-Napoca, Romania. The use of
minimum soil tillage systems within a three years rotation: maize,
soya-bean, wheat favorites the rise of the aggregates hydro stability
with 5.6-7.5% on a 0-20 cm depth and 5-11% on 20-30 cm depth.
The minimum soil tillage systems – paraplow, chisel or rotary grape
– are polyvalent alternatives for basic preparation, germination bed
preparation and sowing, for fields and crops with moderate loose
requirements being optimized technologies for: soil natural fertility
activation and rationalization, reduction of erosion, increasing the
accumulation capacity for water and realization of sowing in the
optimal period. The soil tillage system influences the productivity
elements of cultivated species and finally the productions thus
obtained. Thus, related to conventional working system, the
productions registered in minimum tillage working represented 89-
97% in maize, 103-112% in soya-bean, 93-99% in winter-wheat. The
results of investigations showed that the yield is a conclusion soil
tillage systems influence on soil properties, plant density assurance
and on weed control. Under minimum tillage systems in the case of
winter weat as an option for replacing classic ploughing, the best
results in terms of quality indices were obtained from version worked
with paraplow, followed by rotary harrow and chisel. At variants
worked with paraplow were obtained quality indices close to those of
the variant worked with plow, and protein and gluten content was
even higher. At Ariesan variety, highest protein content, 12.50% and
gluten, 28.6% was obtained for the variant paraplow.
Abstract: The objective of this study was to develop and compare alternative prediction equations of lean meat proportion (LMP) of lamb carcasses. Forty (40) male lambs, 22 of Churra Galega Bragançana Portuguese local breed and 18 of Suffolk breed were used. Lambs were slaughtered, and carcasses weighed approximately 30 min later in order to obtain hot carcass weight (HCW). After cooling at 4º C for 24-h a set of seventeen carcass measurements was recorded. The left side of carcasses was dissected into muscle, subcutaneous fat, inter-muscular fat, bone, and remainder (major blood vessels, ligaments, tendons, and thick connective tissue sheets associated with muscles), and the LMP was evaluated as the dissected muscle percentage. Prediction equations of LMP were developed, and fitting quality was evaluated through the coefficient of determination of estimation (R2 e) and standard error of estimate (SEE). Models validation was performed by k-fold crossvalidation and the coefficient of determination of prediction (R2 p) and standard error of prediction (SEP) were computed. The BT2 measurement was the best single predictor and accounted for 37.8% of the LMP variation with a SEP of 2.30%. The prediction of LMP of lamb carcasses can be based simple models, using as predictors the HCW and one fat thickness measurement.
Abstract: The full length mitochondrial small subunit ribosomal
(mt-rns) gene has been characterized for Ophiostoma novo-ulmi
subspecies americana. The gene was also characterized for
Ophiostoma ulmi and a group II intron was noted in the mt-rns gene
of O. ulmi. The insertion in the mt-rns gene is at position S952 and it
is a group IIB1 intron that encodes a double motif LAGLIDADG
homing endonuclease from an open reading frame located within a
loop of domain III. Secondary structure models for the mt-rns RNA
of O. novo-ulmi subsp. americana and O. ulmi were generated to
place the intron within the context of the ribosomal RNA. The in vivo
splicing of the O.ul-mS952 group II intron was confirmed with
reverse transcription-PCR. A survey of 182 strains of Dutch Elm
Diseases causing agents showed that the mS952 intron was absent in
what is considered to be the more aggressive species O. novo-ulmi
but present in strains of the less aggressive O. ulmi. This observation
suggests that the O.ul-mS952 intron can be used as a PCR-based
molecular marker to discriminate between O. ulmi and O. novo-ulmi
subsp. americana.
Abstract: This study assesses the vulnerability of Bulgarian
agriculture to drought using the WINISAREG model and seasonal
standard precipitation index SPI(2) for the period 1951-2004. This
model was previously validated for maize on soils of different water
holding capacity (TAW) in various locations. Simulations are
performed for Plovdiv, Stara Zagora and Sofia. Results relative to
Plovdiv show that in soils of large TAW (180 mm m-1) net irrigation
requirements (NIRs) range 0-40 mm in wet years and 350-380 mm in
dry years. In soils of small TAW (116 mm m-1), NIRs reach 440 mm
in the very dry year. NIRs in Sofia are about 80 mm smaller. Rainfed
maize is associated with great yield variability (29%
Abstract: This paper presents preliminary results of a
technology assessment analysis for the use of high pressure treatment
(HPT) on Halloumi cheese. In particular, it presents the importance
of this traditional Cyprus cheese to the island-s economy, explains its
production process, and gives a brief introduction to HPT and its
application on cheese. More importantly, it offers preliminary results
of HPT of Halloumi samples and a preliminary economic feasibility
study on the financial implications of the introduction of such
technology.
Abstract: Proteins levels produced by bacteria may be increased
in stressful surroundings, such as in the presence of antibiotics. It
appears that many antimicrobial agents or antibiotics, when used at
low concentrations, have in common the ability to activate or repress
gene transcription, which is distinct from their inhibitory effect.
There have been comparatively few studies on the potential of
antibiotics or natural compounds in nature as a specific chemical
signal that can trigger a variety of biological functions. Therefore,
this study was focusing on the effect of essential oils from
Cymbopogon flexuosus and C. nardus in regulating proteins
production by Bacillus subtilis ATCC 21332. The Minimum
Inhibition Concentrations (MICs) of both essential oils on B. subtilis
were determined by using microdilution assay, resulting 0.2% and
1.56% for each C. flexuosus and C. nardus subsequently. The
bacteria were further exposed to each essential oils at concentration
of 0.01XMIC for 2 days. The proteins were then isolated and
analyzed by sodium dodecyl sulfate polyacrylamide gel
electrophoresis (SDS-PAGE). Protein profile showed that a band
with approximate size of 250 kD was appeared for the treated
bacteria with essential oils. Thus, Bacillus subtilis ATCC 21332 in
stressful condition with the presence of essential oils at low
concentration could induce the protein production.
Abstract: The aim of present study was to assess the effect of
glucogenic (G) and lipogenic (L) diets on blood metabolites in
Baloochi lambs. Three rumen cannulated Baloochi sheep were used
as a 3×3 Latin square design with 3 periods (28 days). Experimental
diets were a glucogenic, a lipogenic and a mixture of G and L diets
(50:50). The animals were fed diets consisted of 50% chopped alfalfa
hay and 50% concentrate. Diets were fed once daily ad libitum.
Blood samples were taken from jugular vein before the feeding, 2, 4
and 6 hour post feeding at day 27. Results indicated that β-
hydroxybutyrate (BHBA), glucose, insulin and aspartate
aminotransferase (AST) were not affected by treatments (P > 0.05).
However, lipogenic diet increased significantly activity of Alanine
aminotransferase (ALT) and concentration of non-esterified fatty acid
(NEFA) in blood plasma (P < 0.05)
Abstract: Forty-five dairy cows were used to compare the
enzyme activity of alkaline phosphatase (ALP), lactate
dehydrogenase (LDH), α -amylase in the cervical mucus of cows
during spontaneous and induced estrus using progestagen or PGF2 α
and to determine whether these enzymes affect the fertility in cows
with induced estrus, at the time of Al. The animals were assigned to 3
groups (no treatment, a Crestar® for 12 days, a double im injection of
PGF2 α). The cows were artificially inseminated (AI). Cervical
mucus samples were collected from all cows 3 to 5 min before the
AI. The results are summarized as follows: ALP and α -amylase
activity for spontaneous estrus were similar to those for induced
estrus (P>0.05) . LDH activity levels during spontaneous and PGF2 α
induced estrus was significantly lower (P < 0.001) than that in
progestagene induced estrus groups. While no difference was found
between the first and the third groups. Our result showed a significant
difference in LDH activity levels between cows conceived with 2 or
more AI and those conceived with 1 AI. The result of this study
showed that the enzyme activity in cervical mucus is helpful for
detection of ovulation and time of AI.
Abstract: Regulatory relationships of 686 intronic miRNA and 784 intergenic miRNAs with mRNAs of 51 intronic miRNA coding genes were established. Interaction features of studied miRNAs with 5'UTR, CDS and 3'UTR of mRNA of each gene were revealed. Functional regions of mRNA were shown to be significantly heterogenous according to the number of binding sites of miRNA and to the location density of these sites.
Abstract: Biochemical investigations were carried out to assess
the effect of different exposure regimes of Kazakhstan crude oil
(KCO) on hepatic antioxidant defense system in albino rats.
Contaminants were delivered under two different dosing regimes,
with all treatments receiving the same total contaminant load by the
end of the exposure period. Rats in regime A injected with KCO
once at a dose of 6 ml/kg bw while in regime B injected multiply at a
dose of 1.5 ml/kg bw on day 1, 3, 5 and 8. Antioxidant biomarkers
were measured in hepatic tissue after 1, 3, 5 and 8 days. Significant
induction was observed in serum aminotransferases (ALT, AST)
(p
Abstract: World population growth drives food demand, promotes intensification of agriculture, development of new production technologies and varieties more suitable for regional nature conditions. Climate change can affect the length of growing period, biomass and carbon accumulation in winter wheat. The increasing mean air temperature resulting from climate change can reduce the length of growth period of cereals, and without adequate adjustments in growing technologies or varieties, can reduce biomass and carbon accumulation. Deeper understanding and effective measures for monitoring and management of cereal growth process are needed for adaptation to changing climate and technological conditions.
Abstract: The present work deals with the stabilisation of
organic clay using hydrated lime. Artificial organic clays were
prepared by adding kaolin and different humic acid contents. Results
given by physical testing show that the presence of humic acid has a
drawback effect on the untreated organic clay. The decrease in
specific gravity value was accompanied by a decrease in dry density
and plasticity of clay at higher humic acid contents. Significant
increase in shear strength at 7 days of curing period is observed in the
lime-treated samples up to 5% lime content. However shear strength
of lime-treated organic clay decreases at longer curing periods. The
results given by laboratory testing is further verified by
microstructure analysis. Based on the results obtained in this study, it
can be concluded that the presence of more than 1.5% humic acid
reduces significantly the efficiency of lime stabilization in organic
clays.
Abstract: In the present study, 49 Hybrid (Catla catla ♂ x
Labeo rohita ♀) were sampled from Al-Raheem Fish Hatchery,
Village Ali Pure Shamali, Jhang Road, 18 Km from Muzaffar Garh
using a cast net and Live fishes were transported to research
laboratory. Mean percentage for water found 79.13 %, ash 6.58 %, fat
2.22 % and protein content 12.06 % in whole wet body weight. It was
observed that body constituents were found increasing in the same
proportion with an increase in body weight while significant
proportional increase was observed with total length. However,
condition factor remained insignificant (P>0.05) with body
constituents.
Abstract: Tofurther advance research on immune-related genes
from T. molitor, we constructed acDNA library and analyzed
expressed sequence taq (EST) sequences from 1,056 clones. After
removing vector sequence and quality checkingthrough thePhred
program (trim_alt 0.05 (P-score>20), 1039 sequences were generated.
The average length of insert was 792 bp. In addition, we identified 162
clusters, 167 contigs and 391 contigs after clustering and assembling
process using a TGICL package. EST sequences were searchedagainst
NCBI nr database by local BLAST (blastx, E