Assessment of Photodynamic Therapy for Staphylococcus Aureus Infected Wounds using Diffuse Reflectance Spectrometry
In this paper we evaluated the efficacy of
photodynamic treatment of infected wounds on pig animal model by
diffuse reflectance spectrometry. The study was conducted on fifteen
wounds contaminated with Staphylococcus aureus bacteria that were
incubated for 30 min with methylene blue solution (c = 3.3 x 10-3 M)
and exposed to laser radiations (λ = 670 nm, P = 15 mW) for 15 min.
The efficiency of photodynamic inactivation of bacteria was
evaluated by microbiological exams and diffuse reflectance
spectrometry. The results of the microbiological exams showed that
the bacterial concentration has decreased from 6.93±0.138
logCFU/ml to 3.12±0.108 logCFU/ml. The spectral examination
showed that the diffuse reflectance of wounds contaminated with
Staphylococcus aureus has decreased from 5.06±0.036 % to
3.36±0.025 %. In conclusion, photodynamic therapy is an effective
method for the treatment of infected wounds and there is a correlation
between the CFU count and diffuse reflectance.
[1] G. Jori, S.B. Brown, "Photosensitized inactivation of microorganisms,"
Photochem Photobiol Sci vol. 3, no. 5, pp. 403-405, 2004.
[2] H.M. Tang, M.R. Hamblin, C.M. Yow. "A comparative in vitro
photoinactivation study of clinical isolates of multidrug-resistant
pathogens,", J. Infect Chemother., vol. 13, no. 2, pp. 87-91, 2007
[3] A. Segalla, C.D. Borsarelli, S.E. Braslavsky, "et al. Photophysical,
photochemical and antibacterial photosensitizing properties of a novel
octacationic Zn(II)-phthalocyanine," Photochem Photobiol Sci. vol. 1,
pp. 641-648, 2002.
[4] P.T.C. Carvalho, A.P.C. Marques, F.A. Reis, "et al. Photodynamic
inactivation of in vitro bacterial cultures from pressure ulcers," Acta Cir
Bras, vol. 21, pp. 32-35, 2006.
[5] K.I. Hajim, D.S. Salih, Y.Z. Rassam, "Laser light combined with a
photosensitizer may eliminate methicillin-resistant strains of
Staphylococcus aureus," Lasers Med Sc, vol. 25, no. 5, pp. 743-748,
2010.
[6] M. Sharma , L. Visai, F. Bragheri, "et al. Toluidine Blue-Mediated
Photodynamic Effects on Staphylococcal Biofilms," Antimicrob Agents
Chemother, vol. 52, pp. 299-305, 2008.
[7] Y. Nitzan, B. Shainberg, Z.Malik, "The mechanism of photodynamic
inactivation of Staphylococcus aureus by deuteroporphyrin," Curr
Microbiol , vol. 19, no. 4, pp. 265-269, 1989.
[8] Y. Nitzan, S. Gozhansky, Z. Malik, "Effect of photoactivated
hematoporphyrin derivative on the viability of Staphylococcus aureus,"
Curr Microbiol, vol. 8, no. 5, pp. 279-284, 1985.
[9] M. Sharma, H. Bansal, P.K. Gupta, "Virulence of Pseudomonas
aeruginosa Cells Surviving Photodynamic Treatment with Toluidine
Blue," Curr Microbiol, vol. 50, pp. 277-280, 2005.
[10] S. Tubby, M. Wilson, S.P. Nair, "Inactivation of staphylococcal
virulence factors using a light-activated antimicrobial agent," BMC
Microbiology, vol. 9, no. 211, pp. 211, 2009.
[11] M.R. Hamblin, D.A. O-Donnell, N. Murthy, "et al. Rapid control of
wound infections by targeted photodynamic therapy monitored by in
vivo bioluminescence imaging," Photochem Photobiol. vol. 75, pp.
51-57, 2002.
[12] M.R. Hamblin, T. Zahra, C.H. Contag, "et al. Optical monitoring and
treatment of potentially lethal wound infections in vivo," J Infect Dis,
vol. 187, pp. 1717-1726, 2003.
[13] O. Simonetti, O. Cirioni, F. Orlando, "et al. Effectiveness of
antimicrobial photodynamic therapy with a single treatment of
RLP068/Cl in an experimental model of Staphylococcus aureus wound
infection," Br J Dermatol, vol. 164, pp. 987-995, 2011.
[14] R.F. Donnelly, C.M. Cassidy, R.G. Loughlin, "et al. Delivery of
Methylene Blue and meso-tetra (N-methyl-4-pyridyl) porphine tetra
tosylate from cross-linked poly(vinyl alcohol) hydrogels: A potential
means of photodynamic therapy of infected wounds," J Photochem
Photobiol B-Biology, vol. 96, no. 3, pp. 223-231, 2009.
[15] S.A.G. Lambrechts SAG, T.N. Demidova, M.C.G. Aalders, "et al.
Photodynamic therapy for Staphylococcus aureus infected burn
wounds in mice. Photochem.," Photobiol. Sci, vol. 4, pp. 503-509,
2005.
[16] X. Ragas, D. Sanchez-Garcia, R. Ruiz-Gonzalez, "et al. Cationic
Porphycenes as Potential Photosensitizers for Antimicrobial
Photodynamic Therapy, J Med Chem, vol. 53, no. 21, pp. 7796 -7803,
2010.
[17] V. Garcia, M. Lima, T. Okamoto, "et al. Effect of photodynamic
therapy on the healing of cutaneous third-degree-burn: histological
study in rats," Lasers Med Sc, vol. 25, no. 2, pp. 221-228, 2010.
[18] M.A. Calin, R.M. Ion, "Optical method for monitoring of
photodynamic inactivation of bacteria", J Biol Phys, vol. 37, pp. 107-
116, 2011.
[19] K. O-Riordan, O.E. Akilov, T. Hasan, "The Potential for Photodynamic
Therapy in the Treatment of Localized Infections," Photodiag
Photodyn Ther, vol. 2, pp. 247-262., 2005.
[20] T.J. Maisch, "Anti-microbial Photodynamic Therapy: Useful in the
Future?" Lasers Med Sci, vol. 22, pp. 83-91, 2007.
[1] G. Jori, S.B. Brown, "Photosensitized inactivation of microorganisms,"
Photochem Photobiol Sci vol. 3, no. 5, pp. 403-405, 2004.
[2] H.M. Tang, M.R. Hamblin, C.M. Yow. "A comparative in vitro
photoinactivation study of clinical isolates of multidrug-resistant
pathogens,", J. Infect Chemother., vol. 13, no. 2, pp. 87-91, 2007
[3] A. Segalla, C.D. Borsarelli, S.E. Braslavsky, "et al. Photophysical,
photochemical and antibacterial photosensitizing properties of a novel
octacationic Zn(II)-phthalocyanine," Photochem Photobiol Sci. vol. 1,
pp. 641-648, 2002.
[4] P.T.C. Carvalho, A.P.C. Marques, F.A. Reis, "et al. Photodynamic
inactivation of in vitro bacterial cultures from pressure ulcers," Acta Cir
Bras, vol. 21, pp. 32-35, 2006.
[5] K.I. Hajim, D.S. Salih, Y.Z. Rassam, "Laser light combined with a
photosensitizer may eliminate methicillin-resistant strains of
Staphylococcus aureus," Lasers Med Sc, vol. 25, no. 5, pp. 743-748,
2010.
[6] M. Sharma , L. Visai, F. Bragheri, "et al. Toluidine Blue-Mediated
Photodynamic Effects on Staphylococcal Biofilms," Antimicrob Agents
Chemother, vol. 52, pp. 299-305, 2008.
[7] Y. Nitzan, B. Shainberg, Z.Malik, "The mechanism of photodynamic
inactivation of Staphylococcus aureus by deuteroporphyrin," Curr
Microbiol , vol. 19, no. 4, pp. 265-269, 1989.
[8] Y. Nitzan, S. Gozhansky, Z. Malik, "Effect of photoactivated
hematoporphyrin derivative on the viability of Staphylococcus aureus,"
Curr Microbiol, vol. 8, no. 5, pp. 279-284, 1985.
[9] M. Sharma, H. Bansal, P.K. Gupta, "Virulence of Pseudomonas
aeruginosa Cells Surviving Photodynamic Treatment with Toluidine
Blue," Curr Microbiol, vol. 50, pp. 277-280, 2005.
[10] S. Tubby, M. Wilson, S.P. Nair, "Inactivation of staphylococcal
virulence factors using a light-activated antimicrobial agent," BMC
Microbiology, vol. 9, no. 211, pp. 211, 2009.
[11] M.R. Hamblin, D.A. O-Donnell, N. Murthy, "et al. Rapid control of
wound infections by targeted photodynamic therapy monitored by in
vivo bioluminescence imaging," Photochem Photobiol. vol. 75, pp.
51-57, 2002.
[12] M.R. Hamblin, T. Zahra, C.H. Contag, "et al. Optical monitoring and
treatment of potentially lethal wound infections in vivo," J Infect Dis,
vol. 187, pp. 1717-1726, 2003.
[13] O. Simonetti, O. Cirioni, F. Orlando, "et al. Effectiveness of
antimicrobial photodynamic therapy with a single treatment of
RLP068/Cl in an experimental model of Staphylococcus aureus wound
infection," Br J Dermatol, vol. 164, pp. 987-995, 2011.
[14] R.F. Donnelly, C.M. Cassidy, R.G. Loughlin, "et al. Delivery of
Methylene Blue and meso-tetra (N-methyl-4-pyridyl) porphine tetra
tosylate from cross-linked poly(vinyl alcohol) hydrogels: A potential
means of photodynamic therapy of infected wounds," J Photochem
Photobiol B-Biology, vol. 96, no. 3, pp. 223-231, 2009.
[15] S.A.G. Lambrechts SAG, T.N. Demidova, M.C.G. Aalders, "et al.
Photodynamic therapy for Staphylococcus aureus infected burn
wounds in mice. Photochem.," Photobiol. Sci, vol. 4, pp. 503-509,
2005.
[16] X. Ragas, D. Sanchez-Garcia, R. Ruiz-Gonzalez, "et al. Cationic
Porphycenes as Potential Photosensitizers for Antimicrobial
Photodynamic Therapy, J Med Chem, vol. 53, no. 21, pp. 7796 -7803,
2010.
[17] V. Garcia, M. Lima, T. Okamoto, "et al. Effect of photodynamic
therapy on the healing of cutaneous third-degree-burn: histological
study in rats," Lasers Med Sc, vol. 25, no. 2, pp. 221-228, 2010.
[18] M.A. Calin, R.M. Ion, "Optical method for monitoring of
photodynamic inactivation of bacteria", J Biol Phys, vol. 37, pp. 107-
116, 2011.
[19] K. O-Riordan, O.E. Akilov, T. Hasan, "The Potential for Photodynamic
Therapy in the Treatment of Localized Infections," Photodiag
Photodyn Ther, vol. 2, pp. 247-262., 2005.
[20] T.J. Maisch, "Anti-microbial Photodynamic Therapy: Useful in the
Future?" Lasers Med Sci, vol. 22, pp. 83-91, 2007.
@article{"International Journal of Biological, Life and Agricultural Sciences:55358", author = "M.A. Calin and D. Voicu and M.R. Calin and D. Savastru and D. Manea", title = "Assessment of Photodynamic Therapy for Staphylococcus Aureus Infected Wounds using Diffuse Reflectance Spectrometry", abstract = "In this paper we evaluated the efficacy of
photodynamic treatment of infected wounds on pig animal model by
diffuse reflectance spectrometry. The study was conducted on fifteen
wounds contaminated with Staphylococcus aureus bacteria that were
incubated for 30 min with methylene blue solution (c = 3.3 x 10-3 M)
and exposed to laser radiations (λ = 670 nm, P = 15 mW) for 15 min.
The efficiency of photodynamic inactivation of bacteria was
evaluated by microbiological exams and diffuse reflectance
spectrometry. The results of the microbiological exams showed that
the bacterial concentration has decreased from 6.93±0.138
logCFU/ml to 3.12±0.108 logCFU/ml. The spectral examination
showed that the diffuse reflectance of wounds contaminated with
Staphylococcus aureus has decreased from 5.06±0.036 % to
3.36±0.025 %. In conclusion, photodynamic therapy is an effective
method for the treatment of infected wounds and there is a correlation
between the CFU count and diffuse reflectance.", keywords = "photodynamic inactivation, bacteria, pigs, wounds", volume = "5", number = "11", pages = "717-4", }
