Abstract: A method which allows a diabetic quadriplegic patient
that has had four limb amputations (above the knee and elbow) to
self-administer injections of insulin has been designed. The aim of
this research project is to improve a quadriplegic patient’s selfmanagement,
affected by diabetes, by designing a suitable device for
self-administering insulin.
The quadriplegic patient affected by diabetes has to be able to selfadminister
insulin safely and independently to guarantee stable
healthy conditions. The device also should be designed to adapt to a
number of different varying personal characteristics such as height
and body weight.
Abstract: The aim of the present study is to investigate the
potential use of the selected seed oils. The oil was extracted using
Soxhlet apparatus and the physicochemical characteristics of the oil
determined using standard methods. The following results were
obtained for the physicochemical parameters analysed: for Egusi seed
oil, Oil yield 53.20%, Saponification value 178.03±1.25 mgKOH/g,
Iodine value 49.10±0.32 g I2/100g, Acid value 4.30±0.86 mgKOH/g,
and Peroxide value 5.80±0.27 meq/kg were obtained. For Pawpaw
seed oil, Oil yield 40.10%, Saponification value 24.13±3.93
mgKOH/g, Iodine value 24.87±0.19 g I2/100g, Acid value 9.46±0.40
mgKOH/g, and Peroxide value 3.12±1.22 meq/kg were obtained. For
Sweet orange seed oil, Oil yield 43.10%, Saponification value
106.30±2.37 mgKOH/g, Iodine value 37.08±0.04 g I2/100g, Acid
value 7.59±0.77 mgKOH/g, and Peroxide value 2.21±0.46 meq/kg
were obtained. From the obtained values of the determined
parameters, the oils can be extracted from the three selected seeds in
commercial quantities and that the egusi and sweet orange seed oils
may be utilized in the industrial soap production.
Abstract: The aim of this work is to present a theoretical analysis of a 2D ultrasound transducer comprised of crossed arrays of metal strips placed on both sides of thin piezoelectric layer (a). Such a structure is capable of electronic beam-steering of generated wavebeam both in elevation and azimuth. In this paper a semi-analytical model of the considered transducer is developed. It is based on generalization of the well-known BIS-expansion method. Specifically, applying the electrostatic approximation, the electric field components on the surface of the layer are expanded into fast converging series of double periodic spatial harmonics with corresponding amplitudes represented by the properly chosen Legendre polynomials. The problem is reduced to numerical solving of certain system of linear equations for unknown expansion coefficients.
Abstract: Ficus deltoidea from the Moraceae family is a popular
medicinal herb in Malaysia. It possesses strong antioxidant and antiinflammatory
properties. In the present study, the anti-inflammatory
effects of F. deltoidea extract on UVB-irradiated HaCaT
Keratinocytes were investigated. HaCaT Keratinocytes were UVBirradiated
(12.5 mJ/cm3) and were treated with 0.05, 0.08 or 0.1% of
F. deltoidea extract. Cell viability following UVB irradiation was
significantly higher in the groups treated with the F. deltoidea extract
at doses of 0.05, 0.08 or 0.1% than in control group with UVB
irradiation only. Tumor necrosis factor-α (TNF-α), interleukin-1α
(IL-1α), interleukin-6 (IL-6) and cyclooxygenase (COX-2) play
primary roles in the inflammation process upon UV irradiation and
are known to be stimulated by UVB irradiation. Treatment with the
F. deltoidea extract dramatically inhibited the UV-induced TNF-α,
IL-1α, IL-6, and COX-2 expression. These results suggest that the F.
deltoidea extract inhibits the production of pro-inflammatory
cytokines and may be an effective protective agent for the treatment
of skin diseases.
Abstract: Heavy metals are one of the major groups of
contaminants in the environment and many of them are toxic even at
very low concentration in plants and animals. However, some metals
play important roles in the biological function of many enzymes in
living organisms. Metals such as zinc, iron, and cooper are important
for survival and activity of enzymes in plants, however heavy metals
can inhibit enzyme which is responsible for defense system of plants.
Polyphenol oxidase (PPO) is a copper-containing metalloenzyme
which is responsible for enzymatic browning reaction of plants.
Enzymatic browning is a major problem for the handling of
vegetables and fruits in food industry. It can be increased and
effected with many different futures such as metals in the nature and
ground. In the present work, PPO was isolated and characterized
from green leaves of red poppy plant (Papaverr hoeas). Then, the
effect of some known antibrowning agents which can form
complexes with metals and metals were investigated on the red poppy
PPO activity. The results showed that glutathione was the most
potent inhibitory effect on PPO activity. Cu(II) and Fe(II) metals
increased the enzyme activities however, Sn(II) had the maximum
inhibitory effect and Zn(II) and Pb(II) had no significant effect on the
enzyme activity. In order to reduce the effect of heavy metals, the
effects of metal-antibrowning agent complexes on the PPO activity
were determined. EDTA and metal complexes had no significant
effect on the enzyme. L-ascorbic acid and metal complexes decreased
but L-ascorbic acid-Cu(II)-complex had no effect. Glutathione–metal
complexes had the best inhibitory effect on Red poppy leaf PPO
activity.
Abstract: Metal-enhanced Luminescence of silicon nanocrystals
(SiNCs) was determined using two different particle sizes of silver
nanoparticles (AgNPs). SiNCs have been characterized by scanning
electron microscopy (SEM), high resolution transmission electron
microscopy (HRTEM), Fourier transform infrared spectroscopy
(FTIR) and X-ray photoelectron spectroscopy (XPS). It is found that
the SiNCs are crystalline with an average diameter of 65 nm and FCC
lattice. AgNPs were synthesized using photochemical reduction of
AgNO3 with sodium dodecyl sulphate (SDS). The enhanced
luminescence of SiNCs by AgNPs was evaluated by confocal Raman
microspectroscopy. Enhancement up to x9 and x3 times were
observed for SiNCs that mixed with AgNPs which have an average
particle size of 100 nm and 30 nm, respectively. Silver NPs-enhanced
luminescence of SiNCs occurs as a result of the coupling between the
excitation laser light and the plasmon bands of AgNPs; thus this
intense field at AgNPs surface couples strongly to SiNCs.
Abstract: The effect of Zn2+, Mg2+, and Ba2+ on Saccharomyces
pastorianus performance was evaluated in this study at independent
and three variable combinations. After 96 h of fermentation, high
wort fermentability (%F) = 29.53 was obtained in medium containing
900:4 ppm Mg2+ + Ba2+. Increased ethanol yield 7.35 %(v/v) and
7.13 %(v/v) were obtained in media containing 900:4 ppm Mg2+ +
Ba2+ and 12:900 ppm Zn2+ + Mg2+. Decrease %F = 22.54 and ethanol
yield 6.18 % (v/v) was obtained in medium containing 12:4 ppm Zn2+
+ Ba2+. In media containing the individual ions, increased %F =
27.94 and 26.03 were recorded for media containing 700 ppm Mg2+
and 2 ppm Ba2+ , with ethanol yield of 7.88% (v/v) and 7.62% (v/v)
respectively. Reduced %F and ethanol yield was observed for 10 ppm
Zn2+ and 4 ppm Ba2+ media. The impact of Ba2+ at 1 and 2 ppm was
significant.
Abstract: A study was conducted to determine the diversity and
abundance of shorebird species habituating the mudflat area of Jeram
Beach and Remis Beach, Selangor, Peninsular Malaysia. Direct
observation technique (using binoculars and video camera) was
applied to record the presence of bird species in the sampling sites
from August 2013 until July 2014. A total of 32 species of shorebird
were recorded during both migratory and non-migratory seasons. Of
these, eleven species (48%) are migrants, six species (26%) have both
migrant and resident populations, four species (17%) are vagrants and
two species (9%) are residents. The compositions of the birds
differed significantly in all months (χ2 = 84.35, p < 0.001). There is a
significant difference in avian abundance between migratory and
non-migratory seasons (Mann-Whitney, t = 2.39, p = 0.036). The
avian abundance were differed significantly in Jeram and Remis
Beaches during migratory periods (t = 4.39, p = 0.001) but not during
non-migratory periods (t = 0.78, p = 0.456). Shorebird diversity was
also affected by tidal cycle. There is a significance difference
between high tide and low tide (Mann-Whitney, t = 78.0, p < 0.005).
Frequency of disturbance also affected the shorebird distribution
(Mann-Whitney, t = 57.0, p = 0.0134). Therefore, this study
concluded that tides and disturbances are two factors that affecting
temporal distribution of shorebird in mudflats area.
Abstract: Mech-Degla, Degla-Beida and Frezza are the date
(Phoenix dactylifera L.) common varieties with a more or less good
availability and feeble trade value. Some morphologic and
physicochemical factors were determined. Results show that the
whole date weight is significantly different (P= 95%) concerning
Mech-Degla and Degla-Beida which are more commercialized than
Frezza whereas the pulp mass proportion in relation to whole fruits is
highest for Frezza (88.28%). Moreover, there is a large variability
concerning the weights and densities of constitutive tissues in each
variety. The white tissue is dominant in Mech-Degla in opposite to
the two other varieties. The variance analyze showed that the
difference in weights between brown and white tissues is significant
(P = 95%) for all studied varieties. Some other morphologic and
chemical proprieties of the whole pulps and their two constitutive
parts (brown or pigmented and white) are also investigated. The
predominance of phenolics in Mech-Degla (4.01g/100g, w.b) and
Frezza (4.96 g/100g, w.b) pulps brown part is the main result
revealed in this study.
Abstract: GRF, Growth regulating factor, genes encode a novel
class of plant-specific transcription factors. The GRF proteins play a
role in the regulation of cell numbers in young and growing tissues
and may act as transcription activations in growth and development
of plants. Identification of GRF genes and their expression are
important in plants to performance of the growth and development of
various organs. In this study, to better understanding the structural
and functional differences of GRFs family, 45 GRF proteins
sequences in A. thaliana, Z. mays, O. sativa, B. napus, B. rapa, H.
vulgare and S. bicolor, have been collected and analyzed through
bioinformatics data mining. As a result, in secondary structure of
GRFs, the number of alpha helices was more than beta sheets and in
all of them QLQ domains were completely in the biggest alpha helix.
In all GRFs, QLQ and WRC domains were completely protected
except in AtGRF9. These proteins have no trans-membrane domain
and due to have nuclear localization signals act in nuclear and they
are component of unstable proteins in the test tube.
Abstract: Preparation of nanoparticles of cerium oxide and
adsorption of bovine serum albumin on them were studied. Particle
size distribution and influence of pH on zeta potential of prepared
CeO2 were determined. Average size of prepared cerium oxide
nanoparticles was 9 nm. The simultaneous measurements of the
bovine serum albumin adsorption and zeta potential determination of
the (adsorption) suspensions were carried out. The adsorption
isotherms were found to be of typical Langmuir type; values of the
bovine serum albumin adsorption capacities were calculated.
Increasing of pH led to decrease of zeta potential and decrease of
adsorption capacity of cerium oxide nanoparticles. The maximum
adsorption capacity was found for strongly acid suspension (am =
118 mg/g). The samples of nanoceria with positive zeta potential
adsorbed more bovine serum albumin on the other hand, the samples
with negative zeta potential showed little or no protein adsorption.
Surface charge or better say zeta potential of CeO2 nanoparticles
plays the key role in adsorption of proteins on such type of materials.
Abstract: Food contamination occurs during post process
handling. This leads to spoilage and growth of pathogenic
microorganisms in the food, thereby reducing its shelf life or
spreading of food borne diseases. Several methods are tried and one
of which is use of antimicrobial packaging. Here, papain, a protease
enzyme, is covalently immobilized with the help of glutarldehyde on
polyurethane and used as a food wrap to protect food from microbial
contamination. Covalent immobilization of papain was achieved at a
pH of 7.4; temperature of 4°C; glutaraldehyde concentration of 0.5%;
incubation time of 24h; and 50mg of papain. The formation of -C=Nobserved
in the Fourier transform infrared spectrum confirmed the
immobilization of the enzyme on the polymer. Immobilized enzyme
retained higher activity than the native free enzyme. The modified
polyurethane showed better reduction of Staphylococcus aureus
biofilm than bare polymer film (eight folds reduction in live colonies,
two times reduction in protein and 6 times reduction in
carbohydrates). The efficacy of this was studied by wrapping it over
S. aureus contaminated cottage cheese (paneer) and cheese and
stored at a temperature of 4°C for 7days. The modified film reduced
the bacterial contamination by eight folds when compared to the bare
film. FTIR also indicated reduction in lipids, sugars and proteins in
the biofilm.
Abstract: The presence of endocrine-disrupting compounds,
such as bisphenol A (BPA), in the environment can cause serious
health problems. However, there are controversial opinions. This
study investigated the reproductive, metabolic, oxidative and
immunologic-disrupting effects of bisphenol A in male rabbits.
Rabbits were divided into five groups. The first four rabbit groups
were administered oral BPA (1, 10, 50, or 100 mg/kg/day) for ten
weeks. The fifth group was administered corn oil as the vehicle. BPA
significantly decreased serum testosterone, estradiol and the free
androgen index (FAI) and significantly increased sex hormone
binding globulin (SHBG) compared with the placebo group. The
higher doses of BPA showed a significant decrease in follicular
stimulating hormone (FSH) and luteinizing hormone (LH). A
significant increase in blood glucose levels was identified in the BPA
groups. The non-significant difference in insulin levels is a novel
finding. The cumulative testicular toxicity of BPA was clearly
demonstrated by the dose-dependent decrease in absolute testes
weight, primary measures of semen quality and a significant increase
in testicular malonaldehyde (MDA). Moreover, BPA significantly
decreased total antioxidant capacity (TAC) and significantly
increased immunoglobulin G (IgG) at the highest concentration. Our
results suggest that BPA, especially at higher doses, is associated
with many adverse effects on metabolism, oxidative stress, immunity,
sperm quality and markers of androgenic action.
Abstract: The biosynthesis of nanoparticles by microorganisms,
on the contrary to chemical synthesis, is an environmentally-friendly
process which has low energy requirements. In this investigation, we
used the microorganism Geobacillus wiegelii, strain GWE1, an
aerobic thermophile belonging to genus Geobacillus, isolated from a
drying oven. This microorganism has the ability to reduce selenite
evidenced by the change of color from colorless to red in the culture.
Elemental analysis and composition of the particles were verified
using transmission electron microscopy and energy-dispersive X-ray
analysis. The nanoparticles have a defined spherical shape and a
selenium elemental state. Previous experiments showed that the
presence of the whole microorganism for the reduction of selenite
was not necessary. The results strongly suggested that an intracellular
NADPH/NADH-dependent reductase mediates selenium
nanoparticles synthesis under aerobic conditions. The enzyme was
purified and identified by mass spectroscopy MALDI-TOF TOF
technique. The enzyme is a 1-pyrroline-5-carboxylate dehydrogenase.
Histograms of nanoparticles sizes were obtained. Size distribution
ranged from 40-160 nm, where 70% of nanoparticles have less than
100 nm in size. Spectroscopic analysis showed that the nanoparticles
are composed of elemental selenium. To analyse the effect of pH in
size and morphology of nanoparticles, the synthesis of them was
carried out at different pHs (4.0, 5.0, 6.0, 7.0, 8.0). For
thermostability studies samples were incubated at different
temperatures (60, 80 and 100 ºC) for 1 h and 3 h. The size of all
nanoparticles was less than 100 nm at pH 4.0; over 50% of
nanoparticles have less than 100 nm at pH 5.0; at pH 6.0 and 8.0 over
90% of nanoparticles have less than 100 nm in size. At neutral pH
(7.0) nanoparticles reach a size around 120 nm and only 20% of them
were less than 100 nm. When looking at temperature effect,
nanoparticles did not show a significant difference in size when they
were incubated between 0 and 3 h at 60 ºC. Meanwhile at 80 °C the
nanoparticles suspension lost its homogeneity. A change in size was
observed from 0 h of incubation at 80ºC, observing a size range
between 40-160 nm, with 20% of them over 100 nm. Meanwhile
after 3 h of incubation at size range changed to 60-180 nm with 50%
of them over 100 nm. At 100 °C the nanoparticles aggregate forming
nanorod structures. In conclusion, these results indicate that is
possible to modulate size and shape of biologically synthesized
nanoparticles by modulating pH and temperature.
Abstract: Taro Scarab beetles (Papuana uninodis, Coleoptera:
Scarabaeidae) inflict severe damage on important root crops and
plants such as Taro or Cocoyam, yam, sweet potatoes, oil palm and
coffee tea plants across Africa and Asia resulting in economic
hardship and starvation in some nations. Scoliid wasps and
Metarhizium anisopliae fungus - bio-control agents; are shown to be
able to control the population of Scarab beetle adults and larvae using
a newly created simulation model based on non-linear ordinary
differential equations that track the populations of the beetle life
cycle stages: egg, larva, pupa, adult and the population of the scoliid
parasitoid wasps, which attack beetle larvae. In spite of the challenge
driven by the longevity of the scarab beetles, the combined effect of
the larval wasps and the fungal bio-control agent is able to control
and drive down the population of both the adult and the beetle eggs
below the environmental carrying capacity within an interval of 120
days, offering the long term prospect of a stable and eco-friendly
environment; where the population of scarab beetles is: regulated by
parasitoid wasps and beneficial soil saprophytes.
Abstract: Nanostructured catalysts were successfully prepared
by acidification of diatomite and regeneration of FCC spent catalysts.
The obtained samples were characterized by IR, XRD, SEM, EDX,
MAS-NMR (27Al and 29Si), NH3-TPD and tested in catalytic
pyrolysis of biomass (rice straw). The results showed that the similar
bio-oil yield of 41.4% can be obtained by pyrolysis with catalysts at
450oC as compared to that of the pyrolysis without catalyst at 550oC.
The bio-oil yield reached a maximum of 42.55% at the pyrolysis
temperature of 500oC with catalytic content of 20%. Moreover, by
catalytic pyrolysis, bio-oil quality was better as reflected in higher
ratio of H/C, lower ratio of O/C. This clearly indicated high
application potential of these new nanostructured catalysts in the
production of bio-oil with low oxygenated compounds.
Abstract: This review summarizes the potential of starch
agroindustrial residues as substrate for biohydrogen production.
Types of potential starch agroindustrial residues, recent developments
and bio-processing conditions for biohydrogen production will be
discussed. Biohydrogen is a clean energy source with great potential
to be an alternative fuel, because it releases energy explosively in
heat engines or generates electricity in fuel cells producing water as
only by-product. Anaerobic hydrogen fermentation or dark
fermentation seems to be more favorable, since hydrogen is yielded
at high rates and various organic waste enriched with carbohydrates
as substrate result in low cost for hydrogen production. Abundant
biomass from various industries could be source for biohydrogen
production where combination of waste treatment and energy
production would be an advantage. Carbohydrate-rich nitrogendeficient
solid wastes such as starch residues can be used for
hydrogen production by using suitable bioprocess technologies.
Alternatively, converting biomass into gaseous fuels, such as
biohydrogen is possibly the most efficient way to use these
agroindustrial residues.
Abstract: Due to the determination of the pollution status of
fresh resources in the Egyptian territorial waters is very important for
public health; this study was carried out to reveal the levels of heavy
metals in the shellfish and their environment and its relation to the
highly developed industrial activities in those areas. A total of 100
shellfish samples from the Rosetta, Edku, El-Maadiya, Abo-Kir and
El-Max coasts [10 crustaceans (shrimp) and 10 mollusks (oysters)]
were randomly collected from each coast. Additionally, 10 samples
from both the water and the sediment were collected from each coast.
Each collected sample was analyzed for cadmium, chromium,
copper, lead and zinc residues using a Perkin Elmer atomic
absorption spectrophotometer (AAS). The results showed that the
levels of heavy metals were higher in the water and sediment from
Abo-Kir. The heavy metal levels decreased successively for the
Rosetta, Edku, El-Maadiya, and El-Max coasts, and the
concentrations of heavy metals, except copper and zinc, in shellfish
exhibited the same pattern. For the concentration of heavy metals in
shellfish tissue, the highest was zinc and the concentrations decreased
successively for copper, lead, chromium and cadmium for all coasts,
except the Abo-Kir coast, where the chromium level was highest and
the other metals decreased successively for zinc, copper, lead and
cadmium. In Rosetta, chromium was higher only in the mollusks,
while the level of this metal was lower in the crustaceans; this trend
was observed at the Edku, El-Maadiya and El-Max coasts as well.
Herein, we discuss the importance of such contamination for public
health and the sources of shellfish contamination with heavy metals.
We suggest measures to minimize and prevent these pollutants in the
aquatic environment and, furthermore, how to protect humans from
excessive intake.
Abstract: In the UK, flooding is responsible for significant
losses to the economy due to the impact on businesses, the vast
majority of which are Small and Medium Enterprises (SMEs).
Businesses of this nature tend to lack formal plans to aid their
response to and recovery from disruptive events such as flooding.
This paper reports on work on how an agent-based model (ABM) is
being developed based on interview data gathered from SMEs at-risk
of flooding and/or have direct experience of flooding. The ABM will
enable simulations to be performed allowing investigations of
different response strategies which SMEs may employ to lessen the
impact of flooding, thus strengthening their resilience.
Abstract: This study presents an attempt to evaluate the
antioxidant potential and antimicrobial activity of methanolic extract,
and essential oils prepared from the leaves of sage (Salvia officinalis
L.). The content of polyphenol in the methanolic extracts from the
leaves of Salvia officinalis was determined spectrophotometrically,
calculated as gallic acid and catechin equivalent. The essential oils
and methanol extract were also subjected to screenings for the
evaluation of their antioxidant activities using 2, 2-diphenyl-1-
picrylhydrazyl (DPPH) test. While the plant essential oils showed
only weak antioxidant activities, its methanol extract was
considerably active in DPPH (IC50 = 37.29 μg/ml) test. Appreciable
total polyphenol content (31.25 mg/g) was also detected for the plant
methanol extract as gallic acid equivalent in the Folin–Ciocalteu test.
The plant was also screened for its antimicrobial activity and good to
moderate inhibitions were recorded for its essential oils, and
methanol extracts against most of the tested microorganisms.
The present investigation revealed that this plant had rich source
of antioxidant properties. It is for this reason that sage has found
increasing application in food formulations.