Abstract: In order to improve the animal protein recommended daily intake of Nigerians, there is an upsurge in breeding of hitherto shunned food animals one of which is the rabbit. Radiography and ultrasonography are tools for diagnosing disease and evaluating the anatomical architecture of parts of the body non-invasively. As the rabbit is becoming a more important food animal, to achieve improved breeding of these animals, the best of the species form a breeding stock and will usually depend on breeding soundness which may be evaluated by assessment of the male reproductive organs by these tools. Four male intact rabbits weighing between 1.2 to 1.5 kg were acquired and acclimatized for 2 weeks. Dorsoventral views of the testes were acquired using a digital radiographic machine and a 5 MHz portable ultrasound scanner was used to acquire images of the testes in longitudinal, sagittal and transverse planes. Radiographic images acquired revealed soft tissue images of the testes in all rabbits. The testes lie in individual scrotal sacs sides on both sides of the midline at the level of the caudal vertebrae and thus are superimposed by caudal vertebrae and the caudal limits of the pelvic girdle. The ultrasonographic images revealed mostly homogenously hypoechogenic testes and a hyperechogenic mediastinum testis. The dorsal and ventral poles of the testes were heterogeneously hypoechogenic and correspond to the epididymis and spermatic cord. The rabbit is unique in the ability to retract the testes particularly when stressed and so careful and stressless handling during the procedures is of paramount importance. The imaging of rabbit testes can be safely done using both imaging methods but ultrasonography is a better method of assessment and evaluation of soundness for breeding.
Abstract: Production of specific antibody responses against hTSH is a cumbersome process due to the high identity between the hTSH and the other members of the glycoprotein hormone family (FSH, LH and HCG) and the high identity between the human hTSH and host animals for antibody production. Therefore, two polyclonal antibodies were purified against two recombinant proteins. Four possible ELISA tests were designed based on these antibodies. These ELISA tests were checked against hTSH and other glycoprotein hormones, and their sensitivity and specificity were assessed. Bioinformatics tools were used to analyze the immunological properties. After the immunogen region selection from hTSH protein, c terminal of B hTSH was selected and applied. Two recombinant genes, with these cut pieces (first: two repeats of C terminal of B hTSH, second: tetanous toxin+B hTSH C terminal), were designed and sub-cloned into the pET32a expression vector. Standard methods were used for protein expression, purification, and verification. Thereafter, immunizations of the white New Zealand rabbits were performed and the serums of them were used for antibody titration, purification and characterization. Then, four ELISA tests based on two antibodies were employed to assess the hTSH and other glycoprotein hormones. The results of these assessments were compared with standard amounts. The obtained results indicated that the desired antigens were successfully designed, sub-cloned, expressed, confirmed and used for in vivo immunization. The raised antibodies were capable of specific and sensitive hTSH detection, while the cross reactivity with the other members of the glycoprotein hormone family was minimum. Among the four designed tests, the test in which the antibody against first protein was used as capture antibody, and the antibody against second protein was used as detector antibody did not show any hook effect up to 50 miu/l. Both proteins have the ability to induce highly sensitive and specific antibody responses against the hTSH. One of the antibody combinations of these antibodies has the highest sensitivity and specificity in hTSH detection.
Abstract: In this article reduced nutritional value of the rabbits’ meat at 1, 1 dimethylhydrazine experimental toxicosis is shown. The assay was performed on liquid chromatograph SHIMADZU LC-20 Prominence (Japan) with fluorometric and spectrophotometric detector. This research has revealed that samples of rabbit meat of the experimental group had significant differences from the control group:in amino acids concentration from 1.2% to 9.1%; vitamin concentration from 11.2% to 60.5%, macro – minerals concentration from 17.4% to 78.1% and saturated fatty acids concentration from 17,1% to 34.5%, respectively. The decrease in the chemical composition of rabbits’ meat at 1,1 dimethylhydrazine toxicosis may be due to changes in the internal processes associated with impaired metabolic homeostasis of animals.
Abstract: This study aimed to assess the in vitro effects of different concentrations of the Viscum album extract on the motility, viability, and reactive oxygen species (ROS) production by rabbit spermatozoa during different time periods (0, 2, and 8h). Spermatozoa motility was assessed by using the CASA (Computer aided sperm analysis) system. Cell viability was evaluated by using the metabolic activity MTT assay, and the luminol-based luminometry was applied to quantify the ROS formation. The CASA analysis revealed that low Viscum concentrations were able to prevent a rapid decline of spermatozoa motility, especially in the case of concentrations ranging between 1 and 5 µg/mL (P
Abstract: Animal nutritionists and scientists have searched for alternative measures to improve the production. One of such alternative is use of organic acids as feed additive in animal nutrition. The study was conducted to investigate the impact of butyric acids, calcium formate, organic acids, and its salts (BCOS) additives on rabbit’s productive performance, carcass traits and meat quality. The study was conducted with 14 Californian breed rabbits. The rabbits were assigned to two treatment groups (seven rabbits per each treatment group). The dietary treatments were 1) control diet, 2) diet supplemented with a mixture BCOS - 2 kg/t of feed. Growth performance characteristics (body weight, daily weight gain, daily feed intake, feed conversion ratio, mortality) were evaluated. Rabbits were slaughtered; carcass characteristics and meat quality were evaluated. Samples loin and hind leg meat were analysed to determine carcass characteristics, pH and colour measurements, cholesterol, and malonyldialdehyde (MDA) content in loin and hind leg meat. Differences between treatments were significant for body weight (1.30 vs. 1.36 kg; P
Abstract: The present in vitro study was designed to reveal whether amygdalin (AMG) is able to cause changes to the motility, viability and mitochondrial activity of rabbit spermatozoa. New Zealand White rabbits (n = 10) aged four months were used in the study. Semen samples were collected from each animal and used for the in vitro incubation. The samples were divided into five equal parts and diluted with saline supplemented with 0, 0.5, 1, 2.5 and 5 mg/mL AMG. At times 0h, 3h and 5h spermatozoa motion parameters were assessed using the SpermVision™ computer-aided sperm analysis (CASA) system, cell viability was examined with the metabolic activity (MTT) assay, and the eosin-nigrosin staining technique was used to evaluate the viability of rabbit spermatozoa. All AMG concentrations exhibited stimulating effects on the spermatozoa activity, as shown by a significant preservation of the motility (P
Abstract: This study evaluated the acute toxicity and tissue
distribution of intravenously administered gold nanoparticles
(AuNPs) in male rabbits. Rabbits were exposed to single dose of
AuNPs (300 μg/ kg). Toxic effects were assessed via general
behavior, hematological parameters, serum biochemical parameters,
and histopathological examination of various rabbits’ organs.
Inductively coupled plasma–mass spectrometry (ICP-MS) was used
to determine gold concentrations in tissue samples collected at
predetermined time intervals. After one week, AuNPs exerted no
obvious acute toxicity in rabbits. However, inflammatory reactions
were observed in liver, lungs and kidneys accompanied with mild
absolute neutrophilia and significant monocytosis. The highest gold
levels were found in the spleen and liver followed by lungs, and
kidneys. These results indicated that AuNPs could be distributed
extensively to various tissues in the body, but primarily in the spleen
and liver.
Abstract: This study was aimed to measure effective transverse
relaxation rates (R2*) in the liver and muscle of normal New Zealand
White (NZW) rabbits. R2* relaxation rate has been widely used in
various hepatic diseases for iron overload by quantifying iron contents
in liver. R2* relaxation rate is defined as the reciprocal of T2*
relaxation time and mainly depends on the constituents of tissue.
Different tissues would have different R2* relaxation rates. The signal
intensity decay in Magnetic resonance imaging (MRI) may be
characterized by R2* relaxation rates. In this study, a 1.5T GE Signa
HDxt whole body MR scanner equipped with an 8-channel high
resolution knee coil was used to observe R2* values in NZW rabbit’s
liver and muscle. Eight healthy NZW rabbits weighted 2 ~ 2.5 kg were
recruited. After anesthesia using Zoletil 50 and Rompun 2% mixture,
the abdomen of rabbit was landmarked at the center of knee coil to
perform 3-plane localizer scan using fast spoiled gradient echo
(FSPGR) pulse sequence. Afterwards, multi-planar fast gradient echo
(MFGR) scans were performed with 8 various echo times (TEs) to
acquire images for R2* measurements. Regions of interest (ROIs) at
liver and muscle were measured using Advantage workstation.
Finally, the R2* was obtained by a linear regression of ln(sı) on TE.
The results showed that the longer the echo time, the smaller the signal
intensity. The R2* values of liver and muscle were 44.8 ± 10.9 s-1 and
37.4 ± 9.5 s-1, respectively. It implies that the iron concentration of
liver is higher than that of muscle. In conclusion, the more the iron
contents in tissue, the higher the R2*. The correlations between R2*
and iron content in NZW rabbits might be valuable for further
exploration.
Abstract: Cancer is still one of the serious diseases threatening
the lives of human beings. How to have an early diagnosis and
effective treatment for tumors is a very important issue. The animal
carcinoma model can provide a simulation tool for the studies of
pathogenesis, biological characteristics, and therapeutic effects.
Recently, drug delivery systems have been rapidly developed to
effectively improve the therapeutic effects. Liposome plays an
increasingly important role in clinical diagnosis and therapy for
delivering a pharmaceutic or contrast agent to the targeted sites.
Liposome can be absorbed and excreted by the human body, and is
well known that no harm to the human body. This study aimed to
compare the therapeutic effects between encapsulated (doxorubicin
liposomal, Lipodox) and un-encapsulated (doxorubicin, Dox)
anti-tumor drugs using magnetic resonance imaging (MRI).
Twenty-four New Zealand rabbits implanted with VX2 carcinoma at
left thighs were classified into three groups: control group (untreated),
Dox-treated group, and LipoDox-treated group, 8 rabbits for each
group. MRI scans were performed three days after tumor implantation.
A 1.5T GE Signa HDxt whole body MRI scanner with a high
resolution knee coil was used in this study. After a 3-plane localizer
scan was performed, three-dimensional (3D) fast spin echo (FSE)
T2-weighted Images (T2WI) was used for tumor volumetric
quantification. Afterwards, two-dimensional (2D) spoiled gradient
recalled echo (SPGR) dynamic contrast-enhanced (DCE) MRI was
used for tumor perfusion evaluation. DCE-MRI was designed to
acquire four baseline images, followed by contrast agent Gd-DOTA
injection through the ear vein of rabbit. A series of 32 images were
acquired to observe the signals change over time in the tumor and
muscle. The MRI scanning was scheduled on a weekly basis for a
period of four weeks to observe the tumor progression longitudinally.
The Dox and LipoDox treatments were prescribed 3 times in the first
week immediately after the first MRI scan; i.e. 3 days after VX2 tumor
implantation. ImageJ was used to quantitate tumor volume and time
course signal enhancement on DCE images. The changes of tumor size
showed that the growth of VX2 tumors was effectively inhibited for
both LipoDox-treated and Dox-treated groups. Furthermore, the tumor
volume of LipoDox-treated group was significantly lower than that of
Dox-treated group, which implies that LipoDox has better therapeutic effect than Dox. The signal intensity of LipoDox-treated group is
significantly lower than that of the other two groups, which implies
that targeted therapeutic drug remained in the tumor tissue. This study
provides a radiation-free and non-invasive MRI method for
therapeutic monitoring of targeted liposome on an animal tumor
model.
Abstract: Current research is targeting new molecular
mechanisms that underlie non-alcoholic fatty liver disease (NAFLD)
and associated metabolic disorders like non-alcoholic steatohepatitis
(NASH). Forty New Zealand White rabbits have been used and fed a
high protein (HP) and energy diet based on grains and containing
11.76 MJ/kg. Boron added to 3 experimental groups’ drinking waters
(30 mg boron/L) as boron compounds. Biochemical analysis
including boron levels, and nuclear magnetic resonance (NMR) based
metabolomics evaluation, and mRNA expression of peroxisome
proliferator-activated receptor (PPAR) family was performed. LDLcholesterol
concentrations alone were decreased in all the
experimental groups. Boron levels in serum and feces were increased.
Content of acetate was in about 2x higher for anhydrous borax group,
at least 3x higher for boric acid group. PPARα mRNA expression
was significantly decreased in boric acid group. Anhydrous borax
attenuated mRNA levels of PPARγ, which was further suppressed by
boric acid. Boron supplementation decreased the degenerative
alterations in hepatocytes. Except borax group other boron groups did
not have a pronounced change in tubular epithels of kidney. In
conclusion, high protein and energy diet leads hepatocytes’
degenerative changes which can be prevented by boron
supplementation. Boric acid seems to be more effective in this
situation.
Abstract: The presence of endocrine-disrupting compounds,
such as bisphenol A (BPA), in the environment can cause serious
health problems. However, there are controversial opinions. This
study investigated the reproductive, metabolic, oxidative and
immunologic-disrupting effects of bisphenol A in male rabbits.
Rabbits were divided into five groups. The first four rabbit groups
were administered oral BPA (1, 10, 50, or 100 mg/kg/day) for ten
weeks. The fifth group was administered corn oil as the vehicle. BPA
significantly decreased serum testosterone, estradiol and the free
androgen index (FAI) and significantly increased sex hormone
binding globulin (SHBG) compared with the placebo group. The
higher doses of BPA showed a significant decrease in follicular
stimulating hormone (FSH) and luteinizing hormone (LH). A
significant increase in blood glucose levels was identified in the BPA
groups. The non-significant difference in insulin levels is a novel
finding. The cumulative testicular toxicity of BPA was clearly
demonstrated by the dose-dependent decrease in absolute testes
weight, primary measures of semen quality and a significant increase
in testicular malonaldehyde (MDA). Moreover, BPA significantly
decreased total antioxidant capacity (TAC) and significantly
increased immunoglobulin G (IgG) at the highest concentration. Our
results suggest that BPA, especially at higher doses, is associated
with many adverse effects on metabolism, oxidative stress, immunity,
sperm quality and markers of androgenic action.
Abstract: Design of experiments made for correlated the hormonal activity of steroids and their effect on the vaginal structure properties. Vaginal wall consists of distinct layers of cells which strongly differ regarding growth in answer to steroid hormones. The presence study carried out for functional evaluation and sustained replacement effect of the reproductive hormones on the vaginal morphometry in the rabbit. Rabbits were maintained control (Co) or ovariectomized (Ovz) and continuously treated with estradiol (Ovz E) and estradiol mixture with testosterone (Ovz ET). Relative to control rabbits with the distinct tissue distribution pattern, ovariectomized animals displayed declined epithelial thickness and atrophy of the muscularis layer that cause the vaginal wall thinning. Estradiol treatment of Orz rabbits inverted these changes to status of the preovariectomy. Histometric measurements in Ovz ET group completely including of thickness of the epithelial and muscular layers was greater in comparison to that of control animals.
Abstract: In the present study, we aimed to design the
intrauterine and extrauterine exposure to 1800 MHz GSM-like RF
radiation and investigate its possible bio-effects on infant female
rabbits. Totally thirty-six New Zealand White female rabbits, onemonth
old, were randomly divided into four groups which are
composed of 9 rabbits; i. Group I [Intrauterine (IU) exposure(-);
Extrauterine (EU) exposure (-)], Group II [IU exposure (-); EU
exposure (+)], Group III [IU exposure(+);EU exposure(-)], Group IV
[IU exposure (+);EU exposure(+)]. The master regulatory enzymes
activities of pentose phosphate pathway (glucose-6-phosphate
dehydrogenase, G-6PD; 6-phosphogluconate dehydrogenase, 6-
PGDH) and glutathione-dependent metabolism (glutathione
peroxidase, GSH-Px; glutathione reductase, GR; glutathione Stransferase,
GST, thioredoxin reductase, TRx) were analyzed in liver
tissues of young female rabbits. Decreased G-6PD, 6-PGD, GSH-Px,
GR activities were found in Group III compared to Group I (p
Abstract: Twenty four New Zealand white rabbits (12 does and
12 bucks) and twenty four Flanders (12 does and 12 bucks) rabbits,
allotted into two feeding regime (6 for each breed, 3 males and 3
females) first one fed commercial ration and second one fed
commercial diet plus sodium butyrate (300 g/ton). The obtained
results showed that at end of 8th week experimental period New
Zealand white rabbits were heavier body weight than Flanders rabbits
(1934.55+39.05 vs. 1802.5+30.99 g); significantly high body weight
gain during experimental period especially during 8th week
(136.1+3.5 vs. 126.8+1.8 g/week); better feed conversion ratio during
all weeks of experiment from first week (3.07+0.16 vs. 3.12+0.10)
till the 8th week of experiment (5.54+0.16 vs. 5.76+0.07) with
significantly high dressing percentages (0.54+0.01 vs. 0.52+0.01).
Also all carcass cuts were significantly high in New Zealand white
rabbits than Flanders. Females rabbits (at the same age) were lower
body weight than males from start of experiment (941.1+39.8
vs.972.1+33.5 g) till the end of experiment (1833.64+37.69 vs.
1903.41+36.93 g); gained less during all weeks of experiment except
during 8th week (132.1+2.3 vs. 130.9+3.4 g/week), with lower
dressing percentage (0.52+0.01 vs. 0.53+0.01) and lighter carcass
cuts than males, however, they had better feed conversion ratio
during 1st week, 7th week and 8th week of experiment. Addition of
300g sodium butyrate/ton of rabbit increased the body weight of
rabbits at the end of experimental period (1882.71+26.45 vs.
1851.5+49.82 g); improve body weight gain at 3rd, 4th, 5th, 6th and
7th week of experiment and significantly improve feed conversion
ratio during all weeks of the experiment from 1st week (2.85+0.07
vs. 3.30+0.15) till the 8th week of the experiment (5.51+0.12 vs.
5.77+0.12). Also the dressing percentage was higher in Sodium
butyrate fed groups than control one (0.53+0.01 vs. 0.52+0.01) and
the most important results of feeding sodium butyrate is the reducing
of the mortality percentage in rabbits during 8 week experiment to
zero percentage as compared with 16% in control group.
Abstract: Steroidal hormones with the efficient changes on the epiphyseal growth plate may influence tissue structure properties. Presents paper to investigate the effects of gonadectomy in the pattern distribution of the epiphyseal structure. Fifteen adult female New Zealand white rabbits were separated into three groups. One group was intact and others groups were selected for surgical operation. From these two groups, one group carried out steroidal administration. The results obtained showed that there is no statistically difference in the mean diameter of the growth plate cells between all three groups. The maximum value of the cartilage cells were allocated to the gonadectomized group and the minimum number were observed in Hormonal induced group significantly. Growth plate height was significantly greater in gonadectomized group than in two other groups.
Abstract: The present study was designed to investigate the
cardio protective role of chronic oral administration of alcoholic
extract of Terminalia arjuna in in-vivo ischemic reperfusion injury
and the induction of HSP72. Rabbits, divided into three groups, and
were administered with the alcoholic extract of the bark powder of
Terminalia arjuna (TAAE) by oral gavage [6.75mg/kg: (T1) and
9.75mg/kg: (T2), 6 days /week for 12 weeks]. In open-chest
Ketamine pentobarbitone anaesthetized rabbits, the left anterior
descending coronary artery was occluded for 15 min of ischemia
followed by 60 min of reperfusion. In the vehicle-treated group,
ischemic-reperfusion injury (IRI) was evidenced by depression of
global hemodynamic function (MAP, HR, LVEDP, peak LV (+) & (-
) (dP/dt) along with depletion of HEP compounds. Oxidative stress
in IRI was evidenced by, raised levels of myocardial TBARS and
depletion of endogenous myocardial antioxidants GSH, SOD and
catalase. Western blot analysis showed a single band corresponding
to 72 kDa in homogenates of hearts from rabbits treated with both the
doses. In the alcoholic extract of the bark powder of Terminalia
arjuna treatment groups, both the doses had better recovery of
myocardial hemodynamic function, with significant reduction in
TBARS, and rise in SOD, GSH, catalase were observed. The results
of the present study suggest that the alcoholic extract of the bark
powder of Terminalia arjuna in rabbit induces myocardial HSP 72
and augments myocardial endogenous antioxidants, without causing
any cellular injury and offered better cardioprotection against
oxidative stress associated with myocardial IR injury.
Abstract: A series of Ti based shape memory alloys with
composition of Ti50Ni49Cr1, Ti50Ni47Cr3 and Ti50Ni45Cr5 were
developed by vacuum arc-melting under a purified argon atmosphere.
The histometric and corrosion evaluation of Ti-Ni-Cr shape memory
alloys have been considered in this research work. The alloys were
developed by vacuum arc melting and implanted subcutaneously in
rabbits for 4, 8 and 12 weeks. Metallic implants were embedded in
order to determine the outcome of implantation on histometric and
corrosion evaluation of Ti-Ni-Cr metallic strips. Encapsulating
membrane formation around the alloys was minimal in the case of all
materials. After histomorphometric analyses it was possible to
demonstrate that there were no statistically significant differences
between the materials. Corrosion rate was also determined in this
study which is within acceptable range. The results showed the Ti-
Ni-Cr alloy was neither cytotoxic, nor have any systemic reaction on
living system in any of the test performed. Implantation shows good
compatibility and a potential of being used directly in vivo system.
Abstract: We aimed to investigate how can target and optimize
pulmonary delivery distribution by changing physicochemical
characteristics of instilled liquid.Therefore, we created a new liquids
group:
a. eligible for desired distribution within lung because of
assorted physicochemical characteristics
b. capable of being augmented with a broad range of
chemicals inertly
c. no interference on respiratory function
d. compatible with airway surface liquid
We developed forty types of new liquid,were composed of
Carboxymethylcellulose sodium,Glycerin and different types of
Polysorbates.Viscosity was measured using a Programmable
Rheometer and surface tension by KRUSS Tensiometer.We
subsequently examined the liquids and delivery protocols by simple
and branched glass capillary tube models of airways.Eventually,we
explored pulmonary distribution of liquids being augmented with
technetium-99m in mechanically ventilated rabbits.We used a single
head large field of view gamma camera.Kinematic viscosity between
0.265Stokes and 0.289Stokes,density between 1g/cm3 and 1.5g/cm3
and surface tension between 25dyn/cm and 35dyn/cm were the most
acceptable.
Abstract: Attachment of the circulating monocytes to the
endothelium is the earliest detectable events during formation of
atherosclerosis. The adhesion molecules, chemokines and matrix
proteases genes were identified to be expressed in atherogenesis.
Expressions of these genes may influence structural integrity of the
luminal endothelium. The aim of this study is to relate changes in the
ultrastructural morphology of the aortic luminal surface and gene
expressions of the endothelial surface, chemokine and MMP-12 in
normal and hypercholesterolemic rabbits. Luminal endothelial
surface from rabbit aortic tissue was examined by scanning electron
microscopy (SEM) using low vacuum mode to ascertain
ultrastructural changes in development of atherosclerotic lesion. Gene
expression of adhesion molecules, MCP-1 and MMP-12 were studied
by Real-time PCR. Ultrastructural observations of the aortic luminal
surface exhibited changes from normal regular smooth intact
endothelium to irregular luminal surface including marked globular
appearance and ruptures of the membrane layer. Real-time PCR
demonstrated differentially expressed of studied genes in
atherosclerotic tissues. The appearance of ultrastructural changes in
aortic tissue of hypercholesterolemic rabbits is suggested to have
relation with underlying changes of endothelial surface molecules,
chemokine and MMP-12 gene expressions.
Abstract: The aim of the present investigation was to compare
sex differences in thyroid gland structure of rabbits. Five adult male
and five adult female (3.1-3.5 kg body weight) New Zealand white
rabbits were used in the experiment. Results showed that at light
microscopic level, there was no sex difference in microscopic
appearance of the thyroid glands. At electron microscopic level,
however, the mitochondria and the microvilli of the follicular cells
are more numerous and the Golgi complex is also more extensive in
male rabbits in comparison to females. Results obtained from
micrometric measurements showed that the volume density of the
follicles is higher in males than in females, but the differences are not
statistically significant .The volume density of epithelium and the
height of follicular cells are significantly greater in males than in
females and reverse is true about the volume density of interstitium
(p