Abstract: Plants can contain a wide variety of substances with
antioxidative properties which are associated with important health
benefits. These positive health effects are of great importance at a
time when the environment is laden with many toxic substances. Five
selected herbal plants namely, Mimosa pudica, Phyllanthus niruri,
Ceiba pentandra, Eleusine polydactyla and Trema amboinensi, were
chosen for the experiment to investigate their total phenolics content
and antioxidant activities using ABTS radical cation decolorization
power, and ferric reducing antioxidant power. The total phenolic
content of each herbal plants ranges from 0.84 to 42.59 mg gallic acid
equivalent/g. The antioxidant activity in the ABTS radical cation
decolorization power varies from 0.005 to 0.362 mg trolox
equivalent/g and the FRAP ranges from 0.30 to 28.42 mg gallic acid
equivalent/g. Among the five medicinal plants, Mimosa pudica has
been an excellent performer in terms of the 3 parameters measured; it
is followed by Phyllanthus niruri. The 5 herbal plants do not have
equivalent antioxidant power. The relative high values for M. pudica
and P. niruri supports the medicinal value of both plants. The total
phenolics, ABTS and FRAP correlate strongly with one another.
Abstract: Cadmium (II) (Cd) is one of the major toxic elemental
pollutants, which is hazardous for humans, animals and plants. γ-
Polyglutamic acid (γ-PGA) is an extracellular biopolymer produced
by several species of Bacillus which has been reported to be an
effective biosorbent for metal ions. The effect of γ-PGA on growth of
rice grown under laboratory conditions was investigated. Rice seeds
were germinated and then grown at 30±1°C on filter paper soaked
with Cd solution and γ-PGA for 7 days. The result showed that Cd
significantly inhibited the growth of roots, shoots by reducing root,
and shoot lengths. Fresh and dry weights also decreased compared
with control; however, the addition of 500 mg·L-1 γ-PGA alleviated
rice seedlings from the adverse effects of Cd. The analysis of
physiological traits revealed that Cd caused a decrease in the total
chlorophyll and soluble protein contents and amylase activities in all
treatments. The Cd content in seedling tissues increased for the Cd
250 μM treatment (P
Abstract: The influence of cultivation factors such as content of ammonium sulfate, glucose and water in the culture medium and particle size of dry orange waste, on their bioconversion for pectinase production was studied using complete factorial design. A polygalacturonase (PG) was isolated using ion exchange chromatography under gradient elution 0-0,5 m/l NaCl (column equilibrate with acetate buffer pH 4,5), subsequently by sephadex G75 column chromatography was applied and the molecular weight was obtained about 51,28 KDa. Purified PG enzyme exhibits a pH and temperature optima of activity at 5 and 35°C respectively. Treatment of apple juice by purified enzyme extract yielded a clear juice, which was competitive with juice yielded by pure Sigma Aldrich Aspergillus niger enzyme.
Abstract: The aims of study were investigation on chemical
composition essential oil and the effect of extract of Coronilla varia
on antimicrobial and cytotoxicity activity. The essential oils of
Coronilla varia is obtained by hydrodistillation and analyzed by
(GC/MS) for determining their chemical composition and
identification of their components. Antibacterial activity of plant
extract was determined by disc diffusion method and anticancer
activity measured by MTT assay. The major components in essential
oil were Caryophyllene Oxide (60.19%), Alphacadinol (4.13%) and
Homoadantaneca Robexylic Acid (3.31%). The extracts from
Coronilla varia had interesting activity against Proteus mirabilis in
the concentration of 700 μg/disc and did not show any activity
against Staphylococus aureus, Bacillus subtillis, Klebsiella
pneumonia and Entrobacter cloacae. The positive control,
Ampicillin, Chloramphenicol and Cenphalothin had shown zone of
inhibition resistant all bacteria. The ethanol extract of Corohilla varia
inhibited on MCF7 cell lines. IC50 0.6(mg/ml) was the optimum
concentration of extract from Coronilla varia inhibition of cell line
growth. The MCF7 cancer cell line and Proteus mirabilis were more
sensitive to Coronilla varia ethanol extract.
Abstract: The high-molecular water-soluble preparations from
several species of two genera (Symphytum and Anchusa) of
Boraginaceae family Symphytum asperum, S. caucasicum, S.officinale
and Anchusa italica were isolated. According to IR, 13C and 1H
NMR, APT, 1D NOE, 2D heteronuclear 1H/13C HSQC and 2D
DOSY experiments, the main chemical constituent of these
preparations was found to be caffeic acid-derived polyether, namely
poly[3-(3,4-dihydroxyphenyl)glyceric acid] (PDPGA) or poly[oxy-1-
carboxy-2-(3,4-dihydroxyphenyl)ethylene]. Most carboxylic groups
of this caffeic acid-derived polymer of A. italica are methylated.
Abstract: Diets high in processed foods have been found to lack
essential micro-nutrients for optimum human development and
overall health. Some micro-nutrients such as copper (Cu) have been
found to enhance the inflammatory response through its oxidative
functions, thereby having a role in cardiovascular disease, metabolic
syndrome, diabetes and related complications. This research study
was designed to determine if food crops could be bio-fortified with
micro-nutrients by growing sprouts on mineral fortified fiber mats. In
the feasibility study described in this contribution, recycled cellulose
fibers and clay, saturated with either micro-nutrient copper ions or
copper nanoparticles, were converted to a novel mineral-cellulose
fiber carrier of essential micro-nutrient and of antimicrobial
properties. Seeds of Medicago sativa (alfalfa), purchased from a
commercial, organic supplier were germinated on engineered
cellulose fiber mats. After the appearance of the first leaves, the
sprouts were dehydrated and analyzed for Cu content. Nutrient
analysis showed ~2 increase in Cu of the sprouts grown on the fiber
mats with copper particles, and ~4 increase on mats with ionic copper
as compared to the control samples. This study illustrates the
potential for the use of engineered mats as a viable way to increase
the micro-nutrient composition of locally-grown food crops and the
need for additional research to determine the uptake, nutritional
implications and risks of micro-nutrient bio-fortification.
Abstract: In order to investigate the prebiotic potential of
oligosaccharides prepared by chemical hydrolysis of water-soluble
polysaccharides (WSP) from Zizyphus lotus leaves, the effect of
oligosaccharides on bacterial growth was studied. The chemical
composition of WSP was evaluated by colorimetric assays revealed
the average values: 7.05±0.73% proteins and 86.21±0.74%
carbohydrates, among them 64.81±0.42% is neutral sugar and the rest
16.25±1.62% is uronic acids. The characterization of
monosaccharides was determined by high performance anion
exchange chromatography with pulsed amperometric detection
(HPAEC-PAD) was found to be composed of galactose (23.95%),
glucose (21.30%), rhamnose (20.28%), arabinose (9.55%), and
glucuronic acid (22.95%). The effects of oligosaccharides on the
growth of lactic acid bacteria were compared with those of fructooligosaccharide
(RP95). The oligosaccharides concentration was
1g/L of Man, Rogosa, Sharpe broth. Bacterial growth was assessed
during 2, 4.5, 6.5, 9, 12, 16 and 24 h by measuring the optical density
of the cultures at 600 nm (OD600) and pH values. During
fermentation, pH in broth cultures decreased from 6.7 to 5.87±0.15.
The enumeration of lactic acid bacteria indicated that
oligosaccharides led to a significant increase in bacteria (P≤0.05)
compared to the control. The fermentative metabolism appeared to be
faster on RP95 than on oligosaccharides from Zizyphus lotus leaves.
Both RP95 and oligosaccharides showed clear prebiotic effects, but
had differences in fermentation kinetics because of to the different
degree of polymerization. This study shows the prebiotic
effectiveness of oligosaccharides, and provides proof for the selection
of leaves of Zizyphus lotus for use as functional food ingredients.
Abstract: As a result of the ambiguity and complexity
surrounding anaerobic digester foaming, efforts have been made by
various researchers to understand the process of anaerobic digester
foaming so as to proffer a solution that can be universally applied
rather than site specific. All attempts ranging from experimental
analysis to comparative review of other process has not fully
explained the conditions and process of foaming in anaerobic
digester. Studying the current available knowledge on foam
formation and relating it to anaerobic digester process and operating
condition, this piece of work presents a succinct and enhanced
understanding of foaming in anaerobic digesters as well as
introducing a simple method to identify the onset of anaerobic
digester foaming based on analysis of historical data from a field
scale system.
Abstract: Anogeissus leiocarpus (Combretaceae) is well known
for its medicinal uses in African traditional medicine, for treating
many human diseases mainly skin diseases and infections. Mycetoma
disease is a fungal and/ or bacterial skininfection, mainly cause by
Madurella mycetomatis fungus. This study was carried out in vitro to
investigate the antifungal activity of Anogeissus leiocarpus leaf
extracts against the isolated pathogenic Madurella mycetomatis, by
using the NCCLS modified method compared to Ketoconazole
standard drug, and MTT assay. The bioactive fraction was subjected
to chemical analysis implementing different chromatographic
analytical methods (TLC, HPLC, and LC-MS/MS). The results
showed significance antifungal activity of A. leiocarpus leaf extracts
against the isolated pathogenic M. mycetomatis, compared to negative
and positive controls. The chloroform fraction showed the highest
antifungal activity. The chromatographic analysis of the chloroform
fraction with the highest activity showed the presence of important
bioactive compounds such as ellagic and flavellagic acids derivatives,
flavonoids and stilbenoid, which are well known for their antifungal
activity.
Abstract: Anaerobic digestion is a well-known technique for
sustainable energy recovery from sewage sludge. However, sewage
sludge digestion is restricted due to certain factors. Pre-treatment
methods have been established in various publications as a promising
technique to improve the digestibility of the sewage sludge and to
enhance the biogas generated which can be used for energy recovery.
In this study, continuous flow microwave (MW) pre-treatment with
different intensities were compared by using 5 L semi-continuous
digesters at a hydraulic retention time of 27 days. We focused on the
effects of MW at different intensities on the sludge solubilization,
sludge digestibility, and biogas production of the untreated and MW
pre-treated sludge. The MW pre-treatment demonstrated an increase
in the ratio of soluble chemical oxygen demand to total chemical
oxygen demand (sCOD/tCOD) and volatile fatty acid (VFA)
concentration. Besides that, the total volatile solid (TVS) removal
efficiency and tCOD removal efficiency also increased during the
digestion of the MW pre-treated sewage sludge compared to the
untreated sewage sludge. Furthermore, the biogas yield also
subsequently increases due to the pre-treatment effect. A higher MW
power level and irradiation time generally enhanced the biogas
generation which has potential for sustainable energy recovery from
sewage treatment plant. However, the net energy balance tabulation
shows that the MW pre-treatment leads to negative net energy production.
Abstract: In the vicinity of red sea about 15 fungi species were
isolated from oil contaminated sites. On the basis of aptitude to
degrade the crude oil and DCPIP assay, two fungal isolates were
selected amongst 15 oil degrading strains. Analysis of ITS-1, ITS-2
and amplicon pyrosequencing studies of fungal diversity revealed
that these strains belong to Penicillium and Aspergillus species. Two
strains that proved to be the most efficient in degrading crude oil was
Aspergillus niger (54%) and Penicillium commune (48%) Subsequent
to two weeks of cultivation in BHS medium the degradation rate
were recorded by using spectrophotometer and GC-MS. Hence, it is
cleared that these fungal strains has capability of degradation and can
be utilize for cleaning the Saudi Arabian environment.
Abstract: The increasing high price of natural gas and oil with attendant increase in energy demand on world markets in recent years has stimulated interest in recovering residual oil saturation across the globe. In order to meet the energy security, efforts have been made in developing new technologies of enhancing the recovery of oil and gas, utilizing techniques like CO2 flooding, water injection, hydraulic fracturing, surfactant flooding etc. Surfactant flooding however optimizes production but poses risk to the environment due to their toxic nature. Amongst proven records that have utilized other type of bacterial in producing biosurfactants for enhancing oil recovery, this research uses a technique to combine biosurfactants that will achieve a scale of EOR through lowering interfacial tension/contact angle. In this study, three biosurfactants were produced from three Bacillus species from freeze dried cultures using sucrose 3 % (w/v) as their carbon source. Two of these produced biosurfactants were screened with the TEMCO Pendant Drop Image Analysis for reduction in IFT and contact angle. Interfacial tension was greatly reduced from 56.95 mN.m-1 to 1.41 mN.m-1 when biosurfactants in cell-free culture (Bacillus licheniformis) were used compared to 4. 83mN.m-1 cell-free culture of Bacillus subtilis. As a result, cell-free culture of (Bacillus licheniformis) changes the wettability of the biosurfactant treatment for contact angle measurement to more water-wet as the angle decreased from 130.75o to 65.17o. The influence of microbial treatment on crushed rock samples was also observed by qualitative wettability experiments. Treated samples with biosurfactants remained in the aqueous phase, indicating a water-wet system. These results could prove that biosurfactants can effectively change the chemistry of the wetting conditions against diverse surfaces, providing a desirable condition for efficient oil transport in this way serving as a mechanism for EOR. The environmental friendly effect of biosurfactants applications for industrial purposes play important advantages over chemically synthesized surfactants, with various possible structures, low toxicity, eco-friendly and biodegradability.
Abstract: The disposal of waste plastics has become a major worldwide environmental problem. Pyrolysis of waste plastics is one of the routes to waste minimization and recycling that has been gaining interest. In pyrolysis, the pyrolysed material is separated into gas, liquid (both are fuel) and solid (char) products. All fractions have utilities and economical value depending upon their characteristics. The first objective of this study is to determine the co-pyrolysis product fractions of waste HDPE- (high density polyethylene) and LDPE (low density polyethylene)-olive pomace (OP) and to determine the qualities of the solid product char. Chars obtained at 700 °C pyrolysis were used in biocomposite preparation as additive. As the second objective, the effects of char on biocomposite quality were investigated. Pyrolysis runs were performed at temperature 700 °C with heating rates of 5 °C/min. Biocomposites were prepared by mixing of chars with bisphenol-F type epoxy resin in various wt%. Biocomposite properties were determined by measuring electrical conductivity, surface hardness, Young’s modulus and tensile strength of the composites. The best electrical conductivity results were obtained with HDPE-OP char. For HDPE-OP char and LDPE-OP char, compared to neat epoxy, the tensile strength values of the composites increased by 102% and 78%, respectively, at 10% char dose. The hardness measurements showed similar results to the tensile tests, since there is a correlation between the hardness and the tensile strength.
Abstract: As genetic diversity is most important for existing, breeding and production of any fish; this study was undertaken for investigating genetic diversity of freshwater mud eel, Monopterus cuchia at population level where three ecological populations such as flooded area of Sylhet (P1), open water of Moulvibazar (P2) and open water of Sunamganj (P3) districts of Bangladesh were considered. Four arbitrary RAPD primers (OPB-12, C0-4, B-03 and OPB-08) were screened and RAPD banding patterns were analyzed among the populations considering 15 individuals of each population. In total 174, 138 and 149 bands were detected in the populations of P1, P2 and P3 respectively; however, each primer revealed less number of bands in each population. 100% polymorphic loci were recorded in P2 and P3 whereas only one monomorphic locus was observed in P1, recorded 97.5% polymorphism. Different genetic parameters such as inter-individual pairwise similarity, genetic distance, Nei genetic similarity, linkage distances, cluster analysis and allelic information, etc. were considered for measuring genetic diversity. The average inter-individual pairwise similarity was recorded 2.98, 1.47 and 1.35 in P1, P2 and P3 respectively. Considering genetic distance analysis, the highest distance 1 was recorded in P2 and P3 and the lowest genetic distance 0.444 was found in P2. The average Nei genetic similarity was observed 0.19, 0.16 and 0.13 in P1, P2 and P3, respectively; however, the average linkage distance was recorded 24.92, 17.14 and 15.28 in P1, P3 and P2 respectively. Based on linkage distance, genetic clusters were generated in three populations where 6 clades and 7 clusters were found in P1, 3 clades and 5 clusters were observed in P2 and 4 clades and 7 clusters were detected in P3. In addition, allelic information was observed where the frequency of p and q alleles were observed 0.093 and 0.907 in P1, 0.076 and 0.924 in P2, 0.074 and 0.926 in P3 respectively. The average gene diversity was observed highest in P2 (0.132) followed by P3 (0.131) and P1 (0.121) respectively.
Abstract: This study was designed to evaluate whether carvacrol
(CAR) could provide protection against lung injury by acute
pancreatitis development. The rats were randomized into groups to
receive (I) no therapy; (II) 50 μg/kg cerulein at 1h intervals by four
intraperitoneal injections (i.p.); (III) 50, 100 and 200 mg/kg CAR by
one i.p.; and (IV) cerulein+CAR after 2h of cerulein injection. 12h
later, serum samples were obtained to assess pancreatic function the
lipase and amylase values. The animals were euthanized and lung
samples were excised. The specimens were stained with
hematoxylin-eosin (H&E), periodic acid–Schif (PAS), Mallory's
trichrome and amyloid. Additionally, oxidative DNA damage was
determined by measuring as increases in 8-hydroxy-deoxyguanosine
(8-OH-dG) adducts. The results showed that the serum activity of
lipase and amylase in AP rats were significantly reduced after the
therapy (p
Abstract: Essential oils of Heracleum persicum (Apiaceae) have been widely used from many years ago, but the difference of its properties among different populations have not been identified up to now. Hydrodistilation Clevenger type was used to obtaining the fruit essential oils of four populations of H. persicum from different localities in Iran, then they were characterized by GC-FID and GC-MS analyses. Some ecological factors were also measured. The oils of four populations were compared to determine the similarities and differences and the relationships between these factors and ecological factors. Based on the result, 18-32 different components were identified in four populations, while the percentage of the main components was higher in population with lower number of components. According to the statistical analyses of chemical components and ecological factors, it can be concluded that some ecological factors such as altitude, less humidity, high difference between day and night temperature and salty soil would lead to lower number of components in essential oil, whereas they consist the higher percentage.
Abstract: The technology of mobile telephony has positively enhanced human life and reports on the bio safety of the radiation from their antennae have been contradictory, leading to serious litigations and violent protests by residents in several parts of the world. The crave for more information, as requested by WHO in order to resolve this issue, formed the basis for this study on the effect of the radiation from 900 MHz GSM antenna on the DNA of Hibiscus sabdariffa. Seeds of H. sabdariffa were raised in pots placed in three replicates at 100, 200, 300 and 400 metres from the GSM antennae in three selected test locations and a control where there was no GSM signal. Temperature (˚C) and the relative humidity (%) of study sites were measured for the period of study (24 weeks). Fresh young leaves were harvested from each plant at two, eight and twenty-four weeks after sowing and the DNA extracts were subjected to RAPD-PCR analyses. There were no significant differences between the weather conditions (temperature and relative humidity) in all the study locations. However, significant differences were observed in the intensities of radiations between the control (less than 0.02 V/m) and the test (0.40-1.01 V/m) locations. Data obtained showed that DNA of samples exposed to rays from GSM antenna had various levels of distortions, estimated at 91.67%. Distortions occurred in 58.33% of the samples between 2-8 weeks of exposure while 33.33% of the samples were distorted between 8-24 weeks exposure. Approximately 8.33% of the samples did not show distortions in DNA while 33.33% of the samples had their DNA damaged twice, both at 8 and at 24 weeks of exposure. The study showed that radiation from the 900 MHz GSM antenna is potent enough to cause distortions to DNA of H. sabdariffa even within 2-8 weeks of exposure. DNA damage was also independent of the distance from the antenna. These observations would qualify emissions from GSM mast as environmental hazard to the existence of plant biodiversities and all life forms in general. These results will trigger efforts to prevent further erosion of plant genetic resources which have been threatening food security and also the risks posed to living organisms, thereby making our environment very safe for our existence while we still continue to enjoy the benefits of the GSM technology.
Abstract: Dunaliella salina has great potential as a system for generating commercially valuable products, including beta-carotene, pharmaceuticals, and biofuels. Our goal is to improve this potential by enhancing growth rate and other properties of D. salina under optimal growth conditions. We used ethyl methane sulfonate (EMS) to generate random mutants in D. salina KU11, a strain classified in Thailand. In a preliminary experiment, we first treated D. salina cells with 0%, 0.8%, 1.0%, 1.2%, 1.44% and 1.66% EMS to generate a killing curve. After that, we randomly picked 30 candidates from approximately 300 isolated survivor colonies from the 1.44% EMS treatment (which permitted 30% survival) as an initial test of the mutant screen. Among the 30 survivor lines, we found that 2 strains (mutant #17 and #24) had significantly improved growth rates and cell number accumulation at stationary phase approximately up to 1.8 and 1.45 fold, respectively, 2 strains (mutant #6 and #23) had significantly decreased growth rates and cell number accumulation at stationary phase approximately down to 1.4 and 1.35 fold, respectively, while 26 of 30 lines had similar growth rates compared with the wild type control. We also analyzed cell size for each strain and found there was no significant difference comparing all mutants with the wild type. In addition, mutant #24 had shown an increase of biomass accumulation approximately 1.65 fold compared with the wild type strain on day 5 that was entering early stationary phase. From these preliminary results, it could be feasible to identify D. salina mutants with significant improved growth rate, cell accumulation and biomass production compared to the wild type for the further study; this makes it possible to improve this microorganism as a platform for biotechnology application.
Abstract: The bioactivity studies from the weed ethanolic crude extracts from leaf, stem, pod and root of wild spider flower; Cleoma viscosa Linn. were analyzed for the growth inhibition of 6 bacterial species; Salmonella typhimurium TISTR 5562, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus TISTR 1466, Streptococcus epidermidis ATCC 1228, Escherichia coli DMST 4212 and Bacillus subtilis ATCC 6633 with initial concentration crude extract of 50 mg/ml. The agar well diffusion results found that the extracts inhibit only gram positive bacteria species; S. aureus, S. epidermidis and B. subtilis. The minimum inhibition concentration study with gram positive strains revealed that leaf crude extract give the best result of the lowest concentration compared with other plant parts to inhibit the growth of S. aureus, S. epidermidis and B. subtilis at 0.78, 0.39 and lower than 0.39 mg/ml, respectively. The determination of total phenolic compounds in the crude extracts exhibited the highest phenolic content was 10.41 mg GAE/g dry weight in leaf crude extract. Analyzed the efficacy of free radical scavenging by using DPPH radical scavenging assay with all crude extracts showed value of IC50 of leaf, stem, pod and root crude extracts were 8.32, 12.26, 21.62 and 35.99 mg/ml, respectively. Studied cytotoxicity of crude extracts on human breast adenocarcinoma cell line by MTT assay found that pod extract had the most cytotoxicity CC50 value, 32.41 µg/ml. Antioxidant activity and cytotoxicity of crude extracts exhibited that the more increase of extract concentration, the more activities indicated. According to the bioactivities results, the leaf crude extract of Cleoma viscosa Linn. is the most interesting plant part for further work to search the beneficial of this weed.
Abstract: This study aims to evaluate the diversity of microorganisms in filters PM2.5 and PM10; and determine the genotoxic and cytotoxic activity of the complex mixture present in PM2.5 filters used in the Aburrá Valley Air Quality Monitoring Network (Colombia). The research results indicate that particulate matter PM2.5 of different monitoring stations are bacteria; however, this study of detection of bacteria and their phylogenetic relationship is not complete evidence to connect the microorganisms with pathogenic or degrading activities of compounds present in the air. Additionally, it was demonstrated the damage induced by the particulate material in the cell membrane, lysosomal and endosomal membrane and in the mitochondrial metabolism; this damage was independent of the PM2.5 concentrations in almost all the cases.