Abstract: Agriculture is the backbone of economy of Pakistan
and cotton is the major agricultural export and supreme source of raw
fiber for our textile industry. To combat severe problems of insect
and weed, combination of three genes namely Cry1Ac, Cry2A and
EPSPS genes was transferred in locally cultivated cotton variety
MNH-786 with the use of Agrobacterium mediated genetic
transformation. The present study focused on the molecular screening
of transgenic cotton plants at T3 generation in order to confirm
integration and expression of all three genes (Cry1Ac, Cry2A and
EPSP synthase) into the cotton genome. Initially, glyphosate spray
assay was used for screening of transgenic cotton plants containing
EPSP synthase gene at T3 generation. Transgenic cotton plants which
were healthy and showed no damage on leaves were selected after 07
days of spray. For molecular analysis of transgenic cotton plants in
the laboratory, the genomic DNA of these transgenic cotton plants
were isolated and subjected to amplification of the three genes. Thus,
seventeen out of twenty (Cry1Ac gene), ten out of twenty (Cry2A
gene) and all twenty (EPSP synthase gene) were produced positive
amplification. On the base of PCR amplification, ten transgenic plant
samples were subjected to protein expression analysis through
ELISA. The results showed that eight out of ten plants were actively
expressing the three transgenes. Real-time PCR was also done to
quantify the mRNA expression levels of Cry1Ac and EPSP synthase
gene. Finally, eight plants were confirmed for the presence and active
expression of all three genes at T3 generation.
Abstract: This paper seeks to analyse the benefits of big data
and more importantly the challenges it pose to the subject of privacy
and data protection. First, the nature of big data will be briefly
deliberated before presenting the potential of big data in the present
days. Afterwards, the issue of privacy and data protection is
highlighted before discussing the challenges of implementing this
issue in big data. In conclusion, the paper will put forward the debate
on the adequacy of the existing legal framework in protecting
personal data in the era of big data.
Abstract: Water contamination by toxic compound is one of the serious environmental problems today. These toxic compounds mostly originated from industrial effluents, agriculture, natural sources and human waste. These studies focus on modification of multiwalled carbon nanotube (MWCNTs) with nanoparticle of calixarene and explore the possibility of using this modification for the remediation of cadmium in water. The nanocomposites were prepared by dissolving calixarene in chloroform solution as solvent, followed by additional multiwalled carbon nanotube (MWCNTs) then sonication process for 3 hour and fabricated the nanocomposites on substrate by spin coating method. Finally, the nanocomposites were tested on cadmium ion (10 mg/ml). The morphology of nanocomposites was investigated by FESEM showing the formation of calixarene on the outer walls of carbon nanotube and cadmium ion also clearly seen from the micrograph. This formation was supported by using energy dispersive x-ray (EDX). The presence of cadmium ions in the films, leads to some changes in the surface potential and Fourier Transform Infrared spectroscopy (FTIR).The nanocomposites MWCNTs-calixarene have potential for development of sensor for pollutant monitoring and nanoelectronics devices applications.
Abstract: Shortfall of electrical energy in Pakistan is a challenge
adversely affecting its industrial output and social growth. As
elsewhere, Pakistan derives its electrical energy from a number of
conventional sources. The exhaustion of petroleum and conventional
resources, the rising costs coupled with extremely adverse climatic
effects are taking its toll especially on the under-developed countries
like Pakistan. As alternate, renewable energy sources like hydropower,
solar, wind, even bio-energy and a mix of some or all of them
could provide a credible alternative to the conventional energy
resources that would not only be cleaner but sustainable as well. As a
model, solar energy-based power grid for the near future has been
attempted to offset the energy shortfalls as a mix with our existing
sustainable natural energy resources. An assessment of solar energy
potential for electricity generation is being presented for fulfilling the
energy demands with higher level of reliability and sustainability.
This model is based on the premise that solar energy potential of
Pakistan is not only reliable but also sustainable. This research
estimates the present & future approaching renewable energy
resource specially the impact of solar energy based power grid for
mitigating energy shortage in Pakistan.
Abstract: The design, manufacture, and operation of thin-walled
pressure vessels must be based on maximum safe operating pressure
and an adequate factor of safety (FoS). This research paper first
reports experimental evaluation of longitudinal and hoops stresses
based on working pressure as well as maximum pressure; and then
includes a critical study of factor of safety (FoS) in the design of a
glass fiber pressure vessel. Experimental work involved the use of
measuring instruments and the readings from pressure gauges.
Design calculations involved the computations of design stress and
FoS; the latter was based on breaking strength of 55 MPa for the
glass fiber (pressure-vessel material). The experimentally determined
FoS value has been critically compared with the general FoS allowed
in the design of glass fiber pressure vessels.
Abstract: Applied industrial engineering is concerned with
imparting employable skills to improve the productivity for current
situation of products and services. The purpose of this case study is to
present the results of an initial research study conducted to identify
the desired professional characteristics of an industrial engineer with
an undergraduate degree and the emerging topic areas that should be
incorporated into the curriculum to prepare industrial engineering
(IE) graduates for the future workforce. Conclusions and
recommendations for applied industrial engineering syllabus have
been gathered and reported below. A two-pronged approach was
taken which included a method of benchmarking by comparing the
applied industrial engineering curricula of various universities and an
industry survey to identify job market requirements. This
methodology produced an analysis of the changing nature of
industrial engineering from learning to practical education. A
curriculum study for engineering is a relatively unexplored area of
research in the Middle East, much less for applied industrial
engineering. This work is an effort to bridge the gap between
theoretical study in the classroom and the real world work
applications in the industrial and service sectors.
Abstract: Domestic goats (Capra hircus) are extremely diverse
species and principal animal genetic resource of the developing
world. These facilitate a persistent supply of meat, milk, fibre, and
skin and are considered as important revenue generators in small
pastoral environments. This study aimed to fingerprint β-LG gene at
PCR-RFLP level in native Saudi goat breeds (Ardi, Habsi and Harri)
in an attempt to have a preliminary image of β-LG genotypic patterns
in Saudi breeds as compared to other foreign breeds such as Indian
and Egyptian. Also, the Phylogenetic analysis was done to investigate
evolutionary trends and similarities among the caprine β-LG gene
with that of the other domestic specie, viz. cow, buffalo and sheep.
Blood samples were collected from 300 animals (100 for each breed)
and genomic DNA was extracted. A fragment of the β-LG gene
(427bp) was amplified using specific primers. Subsequent digestion
with Sac II restriction endonuclease revealed two alleles (A and B)
and three different banding patterns or genotypes i.e. AA, AB and
BB. The statistical analysis showed a general trend that β-LG AA
genotype had higher milk yield than β-LG AB and β-LG BB
genotypes. Nucleotide sequencing of the selected β-LG fragments
was done and submitted to GenBank NCBI (Accession No.
KJ544248, KJ588275, KJ588276, KJ783455, KJ783456 and
KJ874959). Phylogenetic analysis on the basis of nucleotide
sequences of native Saudi goats indicated evolutional similarity with
the GenBank reference sequences of goat, Bubalus bubalis and Bos
taurus. However, the origin of sheep which is the most closely
related from the evolutionary point of view, was located some
distance away.
Abstract: This paper discusses about the findings of preliminary
survey on MATLAB software learning among power electronics
students. One of the main focuses of power electronics course is on
DC to DC boost convertors, because boost convertors are generally
used in different industrial and non industrial applications. Population
samples of this study were randomly selected final year bachelor of
electronics and electrical engineering students from University Tun
Hussein Onn Malaysia (UTHM).As per the results from the survey
questioner analysis, almost eighty percent students are facing
problem and difficulties in Dc to Dc boost convertors experimental
understanding without using MATLAB simulink package. As per
finding of this study it is clear that MATLAB play an effective and
efficient function for better understanding of boost convertors
experimental work among power electronics learners.
Abstract: This paper explains about the voltage output for DC to
DC boost converter between open loop, PID controller and fuzzy
logic controller through Matlab Simulink. Simulink input voltage was
set at 12V and the voltage reference was set at 24V. The analysis on
the deviation of voltage resulted that the difference between reference
voltage setting and the output voltage is always lower. Comparison
between open loop, PID and FLC shows that, the open loop circuit
having a bit higher on the deviation of voltage. The PID circuit
boosts for FLC has a lesser deviation of voltage and proved that it is
such a better performance on control the deviation of voltage during
the boost mode.
Abstract: The study identified the socio-economic and
demographic factors of both married and unmarried females in third
world countries. Almost all the countries have same problems but we
have selected Pakistan as a sample country. The main purpose of this
study was to examine which factors forced women to participate in
labor market. So the best technique of data collection was survey of
both married and unmarried females between the ages of 20 to 49.
Two models (probit and logit) were used to analyze the factors which
effect on FLFP. The result showed that some factors e.g. age;
education and marital status have significant effect on FLFP. The
findings showed that educated women and those who belong to joint
families are more participate because of financial pressure.
Abstract: The effect of trucks on the level of service is
determined by considering passenger car equivalents (PCE) of trucks.
The current version of Highway Capacity Manual (HCM) uses a
single PCE value for all tucks combined. However, the composition
of truck traffic varies from location to location; therefore, a single
PCE value for all trucks may not correctly represent the impact of
truck traffic at specific locations. Consequently, present study
developed separate PCE values for single-unit and combination
trucks to replace the single value provided in the HCM on different
freeways. Site specific PCE values, were developed using concept of
spatial lagging headways (that is the distance between rear bumpers
of two vehicles in a traffic stream) measured from field traffic data.
The study used data from four locations on a single urban freeway
and three different rural freeways in Indiana. Three-stage-leastsquares
(3SLS) regression techniques were used to generate models
that predicted lagging headways for passenger cars, single unit trucks
(SUT), and combination trucks (CT). The estimated PCE values for
single-unit and combination truck for basic urban freeways (level
terrain) were: 1.35 and 1.60, respectively. For rural freeways the
estimated PCE values for single-unit and combination truck were:
1.30 and 1.45, respectively. As expected, traffic variables such as
vehicle flow rates and speed have significant impacts on vehicle
headways. Study results revealed that the use of separate PCE values
for different truck classes can have significant influence on the LOS
estimation.
Abstract: The generalized wave equation models various
problems in sciences and engineering. In this paper, a new three-time
level implicit approach based on cubic trigonometric B-spline for the
approximate solution of wave equation is developed. The usual finite
difference approach is used to discretize the time derivative while
cubic trigonometric B-spline is applied as an interpolating function in
the space dimension. Von Neumann stability analysis is used to
analyze the proposed method. Two problems are discussed to exhibit
the feasibility and capability of the method. The absolute errors and
maximum error are computed to assess the performance of the
proposed method. The results were found to be in good agreement
with known solutions and with existing schemes in literature.
Abstract: This paper presents the voltage problem location
classification using performance of Least Squares Support Vector
Machine (LS-SVM) and Learning Vector Quantization (LVQ) in
electrical power system for proper voltage problem location
implemented by IEEE 39 bus New- England. The data was collected
from the time domain simulation by using Power System Analysis
Toolbox (PSAT). Outputs from simulation data such as voltage, phase
angle, real power and reactive power were taken as input to estimate
voltage stability at particular buses based on Power Transfer Stability
Index (PTSI).The simulation data was carried out on the IEEE 39 bus
test system by considering load bus increased on the system. To verify
of the proposed LS-SVM its performance was compared to Learning
Vector Quantization (LVQ). The results showed that LS-SVM is faster
and better as compared to LVQ. The results also demonstrated that the
LS-SVM was estimated by 0% misclassification whereas LVQ had
7.69% misclassification.
Abstract: Electronic market place plays an important
intermediary role for connecting dealers and retail customers. The
main aim of this paper is to design a value co-creation model in
used-car auctions. More specifically, the study has been designed in
order to describe the process of value co-creation in used-car auctions,
to explore the co-created values in used-car auctions, and finally
conclude the paper indicating the future research directions. Our
analysis shows that economic values as well as non-economic values
are co-created in used-car auctions. In addition, this paper contributes
to the academic society broadening the view of value co-creation in
service science.
Abstract: Recently GPS data is used in a lot of studies to
automatically reconstruct travel patterns for trip survey. The aim is to
minimize the use of questionnaire surveys and travel diaries so as to
reduce their negative effects. In this paper data acquired from GPS and
accelerometer embedded in smart phones is utilized to predict the
mode of transportation used by the phone carrier. For prediction,
Support Vector Machine (SVM) and Adaptive boosting (AdaBoost)
are employed. Moreover a unique method to improve the prediction
results from these algorithms is also proposed. Results suggest that the
prediction accuracy of AdaBoost after improvement is relatively better
than the rest.
Abstract: Digital information is expanding in exponential order in our life. Information that is residing online and offline are stored in huge repositories relating to every aspect of our lives. Getting the required information is a task of retrieval systems. Content based image retrieval (CBIR) is a retrieval system that retrieves the required information from repositories on the basis of the contents of the image. Time is a critical factor in retrieval system and using indexed views with CBIR system improves the time efficiency of retrieved results.
Abstract: This study was conducted to investigate the effect of the leaf essential oil of C. sinensis harvested at 7.00a.m and 4.00p.m on some Liver and Kidney function indices of diabetic rats as well as investigate the effect of time of harvest on the observed effect. Experimental animals were divided into 4 groups (A, B, C and D). Diabetes mellitus was induced in all animals, except the normal control group (Group A), by injecting 150mg/kg body weight of alloxan monohydrate intraperitoneally. Group A received distilled water while group B (diabetic control group) was not treated. Group C and D were treated with leaf essential oil of C. sinensis harvested at 7.00 a.m and 4.00p.m respectively at a dose of 110 mg/kg body weight every other day for 15 days. Alkaline phosphatase (ALP), Alanine Transaminase (ALT) and Aspartate Transaminase (AST) activity was evaluated in the serum, Liver and Kidney of studied animals. Total and Direct Bilirubin level, Total Protein and Globulin, Creatinine and Urea level were also evaluated. Result showed that creatinine and urea, serum ALP, AST and ALT levels was significantly reduced (p < 0.05), while the levels of total Protein and Globulin increased significantly (p < 0.05) for the treated animals compared to the diabetic control group. In conclusion, the leaf essential oil of Citrus sinensis ameliorated the impaired renal and liver function; however, the time of harvest of the leaf does not significantly affect its ameliorative effect.
Abstract: In an interconnected power system, any sudden small
load perturbation in any of the interconnected areas causes the
deviation of the area frequencies, the tie line power and voltage
deviation at the generator terminals. This paper deals with the study
of performance of intelligent Fuzzy Logic controllers coupled with
Conventional Controllers (PI and PID) for Load Frequency Control.
For analysis, an isolated single area and interconnected two area
thermal power systems with and without generation rate constraints
(GRC) have been considered. The studies have been performed with
conventional PI and PID controllers and their performance has been
compared with intelligent fuzzy controllers. It can be demonstrated
that these controllers can successfully bring back the excursions in
area frequencies and tie line powers within acceptable limits in
smaller time periods and with lesser transients as compared to the
performance of conventional controllers under same load disturbance
conditions. The simulations in MATLAB have been used for
comparative studies.
Abstract: The tremendous loss of life that resulted in the aftermath of recent earthquakes in developing countries is mostly due to the collapse of non-engineered and semi-engineered building structures. Such structures are used as houses, schools, primary healthcare centers and government offices. These building are classified structurally into two categories viz. non-engineered and semi-engineered. Non-engineered structures include: adobe, unreinforced masonry (URM) and wood buildings. Semi-engineered buildings are mostly low-rise (up to 3 story) light concrete frame structures or masonry bearing walls with reinforced concrete slab. This paper presents an overview of the typical damage observed in non-engineered structures and their most likely causes in the past earthquakes with specific emphasis on the performance of such structures in the 2005 Kashmir earthquake. It is demonstrated that seismic performance of these structures can be improved from life-safety viewpoint by adopting simple low-cost modifications to the existing construction practices. Incorporation of some of these practices in the reconstruction efforts after the 2005 Kashmir earthquake are examined in the last section for mitigating seismic risk hazard.
Abstract: An experimental study was conducted on foamed concrete with synthetic and natural fibres consisting of AR-glas, polypropylene, steel, kenaf and oil palm fibre. The foamed concrete mixtures produced had a target density of 1000kg/m3 and a mix ratio of (1:1.5:0.45). The fibres were used as additives. The inclusion of fibre was maintained at a volumetric fraction of 0.25 and 0.4%. The water absorption, thermal and shrinkage were determined to study the effect of the fibre on the durability properties of foamed concrete. The results showed that AR-glass fibre has the lowest percentage value of drying shrinkage compared to others.