Abstract: The objective of this study was to investigate the effects of dietary supplementation with raw or heat-treated sunflower oil seed with two levels of 7.5% or 15% on unsaturated fatty acids in milk fat and performances of high-yielding lactating cows. Twenty early lactating Holstein cows were used in a complete randomized design. Treatments included: 1) CON, control (without sunflower oil seed). 2) LS-UT, 7.5% raw sunflower oil seed. 3) LS-HT, 7.5% heat-treated sunflower oil seed. 4) HS-UT, 15% raw sunflower oil seed. 5) HS-HT, 15% heat-treated sunflower oil seed. Experimental period lasted for 4 wk, with first 2 wk used for adaptation to the diets. Supplementation with 7.5% raw sunflower seed (LS-UT) tended to decrease milk yield, with 28.37 kg/d compared with the control (34.75 kg/d). Milk fat percentage was increased with the HS-UT treatment that obtained 3.71% compared with CON that was 3.39% and without significant different. Milk protein percent was decreased high level sunflower oil seed treatments (15%) with 3.18% whereas CON treatment is caused 3.40% protein. The cows fed added low sunflower heat-treated (LS-HT) produced milk with the highest content of total unsaturated fatty acid with 32.59 g/100g of milk fat compared with the HS-UT with 23.59 g/100g of milk fat. Content of C18 unsaturated fatty acids in milk fat increased from 21.68 g/100g of fat in the HS-UT to 22.50, 23.98, 27.39 and 30.30 g/100g of fat from the cow fed HS-HT, CON, LS-UT and LS-HT treatments, respectively. C18:2 isomers of fatty acid in milk were greater by LSHT supplementation with significant effect (P < 0.05). Total of C18 unsaturated fatty acids content was significantly higher in milk of animal fed added low heat-treated sunflower (7.5%) than those fed with high sunflower. In all, results of this study showed that diet cow's supplementation with sunflower oil seed tended to reduce milk production of lactating cows but can improve C18 UFA (Unsaturated Fatty Acid) content in milk fat. 7.5% level of sunflower oil seed that heated seemed to be the optimal source to increase UFA production.
Abstract: This research aims to study consumer acceptance of Tempeh from various raw materials (type of bean) and determine protein contents for comparison. Tempeh made from soybean, peanut, white kidney bean and sesame in the ratio: - soybean:sesame =1:0.1, soybean:white kidney:sesame =1:1:0.1, soybean:peanut:sesame =1:1:0.1 and peanut:white kidney bean: sesame =1:1:0.1. The study found that consumer is most satisfied with appearances on soybean mixed with white kidney and black sesame tempeh (3.98). The most satisfied tempeh with textures is soybean mixed with peanut and black sesame tempeh (4.00). The most satisfied tempeh with odor is peanut mixed with white kidney bean and black sesame tempeh (4.04). And the most satisfied tempeh with flavor is peanut mixed with white kidney bean and black sesame tempeh (4.2). The amount of protein in production, soybean tempeh has the highest protein. When we add sesame seeds, it made the protein content slightly decreased (1.86 and 0.6 %). When we use peanut as raw material, the protein content decreased 15.3%. And when we use
white kidney bean as raw material, the protein content decreased (22.77- 26.11%).
Abstract: Indian subcontinent has a plethora of traditional
medicine systems that provide promising solutions to lifestyle
disorders in an 'all natural way'. Spices and oilseeds hold
prominence in Indian cuisine hence the focus of the current study
was to evaluate the bioactive molecules from Linum usitatissinum
(LU), Lepidium sativum (LS), Nigella sativa (NS) and Guizotia
abyssinica (GA) seeds. The seeds were characterized for functional
lipids like omega-3 fatty acid, antioxidant capacity, phenolic
compounds, dietary fiber and anti-nutritional factors. Analysis of the
seeds revealed LU and LS to be a rich source of α-linolenic acid
(41.85 ± 0.33%, 26.71 ± 0.63%), an omega 3 fatty acid (using
GCMS). While studying antioxidant potential NS seeds demonstrated
highest antioxidant ability (61.68 ± 0.21 TEAC/ 100 gm DW) due to
the presence of phenolics and terpenes as assayed by the Mass
spectral analysis. When screened for anti-nutritional factor
cyanogenic glycoside, LS seeds showed content as high as 1674 ± 54
mg HCN / kg. GA is a probable good source of a stable vegetable oil
(SFA: PUFA 1:2.3). The seeds showed diversified bioactive profile
and hence further studies to use different bio molecules in tandem for
the development of a possible 'nutraceutical cocktail' have been
initiated..
Abstract: Antifungal activities of ether and methanolic extracts of volatiles oils of Nigella Sativa seeds were tested against pathogenic bacterias and fungies strains.The volatile oil were found to have significant antifungal and antibacterial activities compare to tetracycline, cefuroxime and ciprofloxacin positive controls.The ether and methanolic esxtracts were compared to each other for antifungal and antibacterial activities and ether extracts showed stonger activity than methanolic one.
Abstract: This study examined the toxicological effects and
safety of polypeptide k isolated from the seeds of Momordica
charantia in laboratory rats. 30 male Sprague Dawley rats (12 weeks
old, bodyweight 180-200 g) were randomly divided into 3 groups
(1000 mg/kg, 500 mg and 0 mg/kg). Rats were acclimatized to
laboratory conditions for 7 days and at day 8 rats were dosed orally
with polypeptide k (in 2% DMSO/normal saline) and the controls
received the dosed vehicle only. Rats were then observed for 72
hours before sacrificed. Rats were anaesthetized by pentobarbital
(50 mg/kg ip) and 2-3.0 mL of blood was taken by cardiac puncture
and rats were scarified by anaesthetic overdose. Immediately, organs
(heart, lungs, liver, kidneys) were weigh and taken for histology.
Organ sections were then evaluated by a histopathologist. Serum
samples were assayed for liver functions (ALT and γ-GT) and kidney
functions (BUN and creatinine). All rats showed normal behavior
after the dosing and no statistical changes were observed in all blood
parameters and organ weight. Histological examinations revealed
normal organ structures. In conclusion, dosing of rats up to 1000
mg/kg did not have any effects on the rat behavior, liver or kidney
functions nor histology of the selected organs.
Abstract: The effect of plant growth-promoting rhizobacteria
(PGPR) on seed germination, seedling growth and yield of field
grown maize were evaluated in three experiments. In these
experiments six bacterial strains include P.putida strain R-168,
P.fluorescens strain R-93, P.fluorescens DSM 50090, P.putida
DSM291, A.lipoferum DSM 1691, A.brasilense DSM 1690 were
used. Results of first study showed seed Inoculation significantly
enhanced seed germination and seedling vigour of maize. In second
experiment, leaf and shoot dry weight and also leaf surface area
significantly were increased by bacterial inoculation in both sterile
and non-sterile soil. The results showed that inoculation with
bacterial treatments had a more stimulating effect on growth and
development of plants in nonsterile than sterile soil. In the third
experiment, Inoculation of maize seeds with all bacterial strains
significantly increased plant height, 100 seed weight, number of seed
per ear and leaf area .The results also showed significant increase in
ear and shoot dry weight of maize.
Abstract: The effect of magnetic field on germination
characteristics of two wheat Seeds has been studied under laboratory
conditions. Seeds were magnetically exposed to magnetic field
strengths, 125 or 250mT for different periods of time. Mean
germination time and the time required to obtain 10, 25, 50, 75 and
90%of seeds to germinate were calculated. The germination time for
each treatment were in general, higher than corresponding control
values, in the other word in treated seeds time required for mean seed
germination time increased nearly 3 hours in compared non treated
control seeds. T10 for doses D5, D6, D11 and D12 significantly higher
than the control values for both cultivars. Mean germination time
(MGT) in both cultivars significantly increased when the time of
seed exposed at magnetic field treatments increased , about 3 and 2
hour respectively for Omid and BCR cultivars.
Abstract: In order to evaluate the Effects of dual inoculation of
Azotobacter and Mycorrhiza with Nitrogen and Phosphorus levels on
yield and yield components of spring safflower, this study was
carried out in field of Farahan university in Markazi province in
2007. A factorial in a randomized complete block design with three
replications was used inoculation of Azotobacter (with inoculation
and without inoculation) and Mycorrhiza (with inoculation and
without inoculation ) with Nitrogen and Phosphorus levels [F0= N0+
P0 (kg.ha-1), F1= N50+ P25(kg.ha-1), F2= N100+ P50(kg.ha-1) and
F3= N150+ P75 (kg.ha-1)] on spring safflower (cultivar IL-111). In
this study characteristics such as: Harvest index, Hectolitre weight,
Root dry weight, Seed yield, Mycorrhizal Colonization Root,
Number of days to maturity were assessed. Results indicated that
treatment (A0M1F3) with grain yield (1556 kg.ha-1) and treatment
(A0M1F0) with grain yield (918 kg.ha-1) were significantly superior
to the other treatments and according to calculated, inoculation seeds
in plantig date with Azotobacter and Mycorrhiza to cause increase
grain yield about 5/38 percentage. we can by inoculation safflower
seeds with Azotobacter and Mycorrhiza too easily at the time sowing
date. The purpose of this research, study and evaluation the role of
biological fixation N and P, to provide for feeds plants.
Abstract: A pot experiment was carried out under controlled
conditions to evaluate the residual effects of different doses of
atrazine+alachlor and foramsulfuron used in corn fields on the
growth and physiology of rapeseed (Brassica napus L.). A split-plot
experiment in CRD with 4 replications was used. The main plots
consisted of herbicide type (atrazine+alachlor mixture and
foramsulfuron) and the sub-plots were different residual doses of the
herbicides (0, 1%, 5%, 10%, 20%, 40%, 50% and 100%). 7 cm
diameter pots were filled with a virgin soil and seeds of rapeseed cv.
Hayola were planted in them. The pots were kept under controlled
conditions for 8 weeks after germination. At harvest, the growth
parameters and the chlorophyll contents of the leaves were
determined. The results showed that the growth of rapeseed plants
was completely prevented at the highest residual doses of the
herbicides (50 and 100 %). The growth parameters of rapeseed plants
were affected by all doses of both types of the herbicide as compared
to the controls. The residual effects of atrazine+alachlor mixture in
reducing the growth parameters of rapeseed were more pronounced
as compared to the residual effects of foramsulfuron alone.
Abstract: An experiment was conducted in October 2008 due the ability replacement plant associate biofertilizers by chemical fertilizers and the qualifying rate of chemical N fertilizers at the moment of using this biofertilizers and the interaction of this biofertilizer on each other. This field experiment has been done in Persepolis (Throne of Jamshid) and arrange by using factorial with the basis of randomized complete block design, in three replication Azespirilium SP bacteria has been admixed with consistence 108 cfu/g and inoculated with seeds of wheat, The streptomyces SP has been used in amount of 550 gr/ha and concatenated on clay and for the qualifying range of chemical fertilizer 4 level of N chemical fertilizer from the source of urea (N0=0, N1=60, N2=120, N3=180) has been used in this experiment. The results indicated there were Significant differences between levels of Nitrogen fertilizer in the entire characteristic which has been measured in this experiment. The admixed Azespirilium SP showed significant differences between their levels in the characteristics such as No. of fertile ear, No. of grain per ear, grain yield, grain protein percentage, leaf area index and the agronomic fertilizer use efficiency. Due the interaction streptomyses with Azespirilium SP bacteria this actinomycet didn-t show any statistically significant differences between it levels.
Abstract: The selection for plantation of a particular type of
mustard plant depending on its productivity (pod yield) at the stage
of maturity. The growth of mustard plant dependent on some
parameters of that plant, these are shoot length, number of leaves,
number of roots and roots length etc. As the plant is growing, some
leaves may be fall down and some new leaves may come, so it can
not gives the idea to develop the relationship with the seeds weight at
mature stage of that plant. It is not possible to find the number of
roots and root length of mustard plant at growing stage that will be
harmful of this plant as roots goes deeper to deeper inside the land.
Only the value of shoot length which increases in course of time can
be measured at different time instances. Weather parameters are
maximum and minimum humidity, rain fall, maximum and minimum
temperature may effect the growth of the plant. The parameters of
pollution, water, soil, distance and crop management may be
dominant factors of growth of plant and its productivity. Considering
all parameters, the growth of the plant is very uncertain, fuzzy
environment can be considered for the prediction of shoot length at
maturity of the plant. Fuzzification plays a greater role for
fuzzification of data, which is based on certain membership
functions. Here an effort has been made to fuzzify the original data
based on gaussian function, triangular function, s-function,
Trapezoidal and L –function. After that all fuzzified data are
defuzzified to get normal form. Finally the error analysis
(calculation of forecasting error and average error) indicates the
membership function appropriate for fuzzification of data and use to
predict the shoot length at maturity. The result is also verified using
residual (Absolute Residual, Maximum of Absolute Residual, Mean
Absolute Residual, Mean of Mean Absolute Residual, Median of
Absolute Residual and Standard Deviation) analysis.
Abstract: Evaluation of crop plants resistance to environmental
stresses specially in germination stage is a critical factor in their
selection in different conditions of cultivation. Therefore use of a
procedure in controllable situation can help to evaluate plants
reaction to stress quickly and precisely. In order to study germination
characteristics of flax in water and salinity stress conditions were
conducted two laboratories experimental. The two experimental were
conducted in 4-replicant completing random design for salinity and
water stress. The treatment, for salinity and water stress was three
potential (zero, 40, 80 mM) of NaCl and three potential (zero, -2, -4
bar) of PEG respectively. Germination percentage and rate, in
addition to Radical and plumule length and dry-weight and
plumule/Radical ration were measured. All of characteristics reduce
under water stress conditions. salinity stress significant reduce
germination rate and Radical and plumule length of flax seeds.
Hydropriming and osmopriming significant increased germination
rate, plumule length and plumule/Radical ration ration of flax seeds.
But germination percentage and Radical and plumule dry weight
significant increased only in hydropriming treat. Hydropriming and
osmopriming could not be used to improved germination under saline
and drought stress. But has more tolerance in salinity and drought
stress in flax by less reduce in Radical and plumule length under
saline and drought stress.
Abstract: The seeds of cotton (Gossypium hirsutum) fall among the lesser known oil seeds. Cottonseeds are not normally consumed in their natural state due to their gossypol content, an antinutrient. The effect of processing on the sensory characteristics and chemical composition of cottonseed and its extract was studied by subjecting the cottonseed extract to heat treatment (boiling) and the cottonseed to fermentation. The cottonseed extract was boiled using the open pot and the pressure pot for 30 minutes respectively. The fermentation of the cottonseed was carried out for 6 days with samples withdrawn at intervals of 2 days. The extract and fermented samples were subjected to chemical analysis and sensory evaluated for colour, aroma, taste, mouth feel, appearance and overallacceptability. The open pot sample was more preferred. Fermentation for 6 days resulted into a significant reduction in gossypol level of the cottonseed; however, sample fermented for 2 days was most preferred.
Abstract: Neem is a highly heterozygous and commercially
important perennial plant. Conventionally, it is propagated by seeds
which loose viability within two weeks. Strictly cross pollinating
nature of the plant causes serious barrier to the genetic improvement
by conventional methods. Alternative methods of tree improvement
such as somatic hybridization, mutagenesis and genetic
transformation require an efficient in vitro plant regeneration system.
In this regard, somatic embryogenesis particularly secondary somatic
embryogenesis may offer an effective system for large scale plant
propagation without affecting the clonal fidelity of the regenerants. It
can be used for synthetic seed production, which further bolsters
conservation of this tree species which is otherwise very difficult
The present report describes the culture conditions necessary to
induce and maintain repetitive somatic embryogenesis, for the first
time, in neem. Out of various treatments tested, the somatic embryos
were induced directly from immature zygotic embryos of neem on
MS + TDZ (0.1 μM) + ABA (4 μM), in more than 76 % cultures.
Direct secondary somatic embryogenesis occurred from primary
somatic embryos on MS + IAA (5 μM) + GA3 (5 μM) in 12.5 %
cultures. Embryogenic competence of the explant as well as of the
primary embryos was maintained for a long period by repeated
subcultures at frequent intervals. A maximum of 10 % of these
somatic embryos were converted into plantlets.
Abstract: Recent research on seeds of bio-diesel plants like
Jatropha curcas, constituting 40-50% bio-crude oil indicates its
potential as one of the most promising alternatives to conventional
sources of energy. Also, limited studies on utilization of de-oiled cake
have revealed that Jatropha bio-waste has good potential to be used as
organic fertilizers produced via aerobic and anaerobic treatment.
However, their commercial exploitation has not yet been possible. The
present study aims at developing appropriate bio-processes and
formulations utilizing Jatropha seed cake as organic fertilizer, for
improving the growth of Polianthes tuberose L. (Tuberose). Pot
experiments were carried out by growing tuberose plants on soil
treated with composted formulations of Jatropha de-oiled cake, Farm
Yard Manure (FYM) and inorganic fertilizers were also blended in
soil. The treatment was carried out through soil amendment as well as
foliar spray. The growth and morphological parameters were
monitored for entire crop cycle.
The growth Length and number of leaves, spike length, rachis
length, number of bulb per plant and earliness of sprouting of bulb and
yield enhancement were comparable to that achieved under inorganic
fertilizer. Furthermore, performance of inorganic fertilizer also showed
an improvement when blended with composted bio-waste. These
findings would open new avenues for Jatropha based bio-wastes to be
composted and used as organic fertilizers for commercial floriculture.
Abstract: Among the many promising nanomaterials with antifungal properties, metal nanoparticles (silver nanoparticles) stand out due to their high chemical activity. Therefore, the aim of this study was to evaluate the effect of silver nanoparticles (AgNPs) against Phomopsis sp. AgNPs were synthesized by silver nitrate reduction with sodium citrate and stabilized with ammonia. The synthesized AgNPs have further been characterized by UV/Visible spectroscopy, Biophysical techniques like Dynamic light scattering (DLS) and Scanning Electron Microscopy (SEM). The average diameter of the prepared silver colloidal nanoparticles was about 52 nm. Absolute inhibitions (100%) were observed on treated with a 270 and 540 µg ml-1 concentration of AgNPs. The results from the study of the AgNPs antifungal effect are significant and suggest that the synthesized silver nanoparticles may have an advantage compared with conventional fungicides.
Abstract: The aim of the present study was to investigate the
chemical and biological properties of local cowpea seed protein
cultivated in Gizan region. The results showed that the cowpea and
its products contain high level of protein (22.9-77.6%), high
carbohydrates (9.4-64.3%) and low fats (0.1-0.3%). The trypsin and
chymotrypsin activities were found to be 32.2 and 15.2 units,
respectively. These activities were not affected in both defatted and
protein concentrate whereas they were significantly reduced in
isolated protein and cooked samples. The phytate content of cooked
and concentrated cowpea samples varied from 0.25% -0.32%,
respectively. Tannin content was found to be 0.4% and 0.23% for
cooked and raw samples, respectively. The in vitro protein
digestibility was very high in cowpea seeds (75.04-78.76%). The
biological evaluation using rats showed that the group fed with
animal feed containing casein gain more weight than those fed with
that containing cowpea. However, the group fed with cooked cowpea
gain more weight than those fed with uncooked cowpea. On the
other hand, in vivo digestion showed high value (98.33%) among the
group consumed casein compared to other groups those consumed
cowpea contains feed. This could be attributed to low antinutritional
factors in casein contains feed compared to those of cowpea contains
feed because cooking significantly increased the digestion rate
(80.8% to 83.5%) of cowpea contains feed. Furthermore, the
biological evaluation was high (91.67%) of casein containing feed
compared to that of cowpea containing feed (80.83%-87.5%). The
net protein utilization (NPU) was higher (89.67%) in the group fed
with casein containing feed than that of cowpea containing feed
(56.33%-69.67%).
Abstract: Ozone (O3) is considered as one of the most
phytotoxic pollutants with deleterious effects on living and non living
components of Ecosystems. It reduces growth and yield of many
crops as well as alters the physiology and crop quality. The present
study described series of experiments to investigate the effects of
ambient O3 at different locations with different ambient levels of O3
depending on proximity to pollutant source and ranged between 17
ppb/h in control experiment to 112 ppb/h in industrial area
respectively. The ambient levels in other three locations (King Saud
University botanical garden, King Fahd Rd, and Almanakh Garden)
were 61,61,77 ppb/h respectively. Tow legume crops species (vicia
vaba L ; and Pisum sativum) differ in their phenology and sensitivity
were used. The results showed a significant negative effect to ozone
on morphology, number of injured leaves, growth and productivity
with a difference in the degree of response depending on the plant
type. Visia Faba showed sensitivity to ozone to number and leaf area
and the degree of injury leaves 3, pisum sativum show higher
sensitivity for the gas for degree of injury 1,The relative growth rate
and seed weight, it turns out there is no significant difference
between the two plants in plant height and number of seeds.
Abstract: The influences of pulsed electric fields on early
physiological development in Arabidopsis thaliana were studied.
Inside a 4-mm electroporation cuvette, pre-germination seeds were
subjected to high-intensity, nanosecond electrical pulses generated
using laboratory-assembled pulsed electric field system. The field
strength was varied from 5 to 20 kV.cm-1 and the pulse width and the
pulse number were maintained at 10 ns and 100, respectively,
corresponding to the specific treatment energy from 300 J.kg-1 to 4.5
kJ.kg-1. Statistical analyses on the average leaf area 5 and 15 days
following pulsed electric field treatment showed that the effects
appear significant the second week after treatments with a maximum
increase of 80% compared to the control (P < 0.01).
Abstract: In order to study of hydropriming and halopriming on
germination and early growth stage of wheat (Triticum aestivum) an
experiment was carried out in laboratory of the Department of
Agronomy and Plant breeding, Shahrood University of Technology.
Seed treatments consisted of T1: control (untreated seeds), T2:
soaking in distilled water for 18 h (hydropriming). T3: soaking in -
1.2 MPa solution of CaSO4 for 36 h (halopriming). Germination and
early seedling growth were studied using distilled water (control) and
under osmotic potentials of -0.4, -0.8 and -1.2 MPa for NaCl and
polyethylene glycol (PEG 6000), respectively. Results showed that
Hydroprimed seeds achieved maximum germination seedling dry
weight, especially during the higher osmotic potentials. Minimum
germination was recorded at untreated seeds (control) followed by
osmopriming. Under high osmotic potentials, hydroprimed seeds had
higher GI (germination index) as compared to haloprimed or
untreated seeds. Interaction effect of seed treatment and osmotic
potential significantly affected the seedling vigour index (SVI).