Abstract: The influences of pulsed electric fields on early
physiological development in Arabidopsis thaliana were studied.
Inside a 4-mm electroporation cuvette, pre-germination seeds were
subjected to high-intensity, nanosecond electrical pulses generated
using laboratory-assembled pulsed electric field system. The field
strength was varied from 5 to 20 kV.cm-1 and the pulse width and the
pulse number were maintained at 10 ns and 100, respectively,
corresponding to the specific treatment energy from 300 J.kg-1 to 4.5
kJ.kg-1. Statistical analyses on the average leaf area 5 and 15 days
following pulsed electric field treatment showed that the effects
appear significant the second week after treatments with a maximum
increase of 80% compared to the control (P < 0.01).
Abstract: Brassinosteroids (BRs) regulate cell elongation,
vascular differentiation, senescence, and stress responses. BRs signal
through the BES1/BZR1 family of transcription factors, which
regulate hundreds of target genes involved in this pathway. In this
research a comprehensive genome-wide analysis was carried out in
BES1/BZR1 gene family in Arabidopsis thaliana, Cucumis sativus,
Vitis vinifera, Glycin max and Brachypodium distachyon.
Specifications of the desired sequences, dot plot and hydropathy plot
were analyzed in the protein and genome sequences of five plant
species. The maximum amino acid length was attributed to protein
sequence Brdic3g with 374aa and the minimum amino acid length
was attributed to protein sequence Gm7g with 163aa. The maximum
Instability index was attributed to protein sequence AT1G19350
equal with 79.99 and the minimum Instability index was attributed to
protein sequence Gm5g equal with 33.22. Aliphatic index of these
protein sequences ranged from 47.82 to 78.79 in Arabidopsis
thaliana, 49.91 to 57.50 in Vitis vinifera, 55.09 to 82.43 in Glycin
max, 54.09 to 54.28 in Brachypodium distachyon 55.36 to 56.83 in
Cucumis sativus. Overall, data obtained from our investigation
contributes a better understanding of the complexity of the
BES1/BZR1 gene family and provides the first step towards directing
future experimental designs to perform systematic analysis of the
functions of the BES1/BZR1 gene family.
Abstract: Eukaryotic protein-coding genes are interrupted by spliceosomal introns, which are removed from the RNA transcripts before translation into a protein. The exon-intron structures of different eukaryotic species are quite different from each other, and the evolution of such structures raises many questions. We try to address some of these questions using statistical analysis of whole genomes. We go through all the protein-coding genes in a genome and study correlations between the net length of all the exons in a gene, the number of the exons, and the average length of an exon. We also take average values of these features for each chromosome and study correlations between those averages on the chromosomal level. Our data show universal features of exon-intron structures common to animals, plants, and protists (specifically, Arabidopsis thaliana, Caenorhabditis elegans, Drosophila melanogaster, Cryptococcus neoformans, Homo sapiens, Mus musculus, Oryza sativa, and Plasmodium falciparum). We have verified linear correlation between the number of exons in a gene and the length of a protein coded by the gene, while the protein length increases in proportion to the number of exons. On the other hand, the average length of an exon always decreases with the number of exons. Finally, chromosome clustering based on average chromosome properties and parameters of linear regression between the number of exons in a gene and the net length of those exons demonstrates that these average chromosome properties are genome-specific features.