Abstract: Brassinosteroids (BRs) regulate cell elongation,
vascular differentiation, senescence, and stress responses. BRs signal
through the BES1/BZR1 family of transcription factors, which
regulate hundreds of target genes involved in this pathway. In this
research a comprehensive genome-wide analysis was carried out in
BES1/BZR1 gene family in Arabidopsis thaliana, Cucumis sativus,
Vitis vinifera, Glycin max and Brachypodium distachyon.
Specifications of the desired sequences, dot plot and hydropathy plot
were analyzed in the protein and genome sequences of five plant
species. The maximum amino acid length was attributed to protein
sequence Brdic3g with 374aa and the minimum amino acid length
was attributed to protein sequence Gm7g with 163aa. The maximum
Instability index was attributed to protein sequence AT1G19350
equal with 79.99 and the minimum Instability index was attributed to
protein sequence Gm5g equal with 33.22. Aliphatic index of these
protein sequences ranged from 47.82 to 78.79 in Arabidopsis
thaliana, 49.91 to 57.50 in Vitis vinifera, 55.09 to 82.43 in Glycin
max, 54.09 to 54.28 in Brachypodium distachyon 55.36 to 56.83 in
Cucumis sativus. Overall, data obtained from our investigation
contributes a better understanding of the complexity of the
BES1/BZR1 gene family and provides the first step towards directing
future experimental designs to perform systematic analysis of the
functions of the BES1/BZR1 gene family.
Abstract: Transcription factor p53 has a powerful tumor
suppressing function that is associated with many cancers. However,
p53 of the molecular weight was higher make the limitation across to
skin or cell membrane. Thymidine dinucleotide (pTT), an
oligonucleotide, can activate the p53 transcription factor. pTT is a
hydrophilic and negative charge oligonucleotide, which delivery in to
cell membrane need an appropriate carrier. The aim of this study was
to improve the bioavailability of the nucleotide fragment, thymidine
dinucleotide (pTT), using elasic liposome carriers to deliver the drug
into the skin. The study demonstrate that dioleoylphosphocholine
(DOPC) incorporated with sodium cholate at molar ratio 1:1 can
archived the particle size about 220 nm. This elastic liposome could
penetration through skin from stratum corneum to whole epidermis by
confocal laser scanning microscopy (CLSM). Moreover, we observed
the the slight increase in generation of p53 by western blot.
Abstract: Candida albicans ATCC 10231 had low endogenous activity of the alternative oxidase compared with that of C. albicans ATCC 10261. In C. albicans ATCC 10231 the endogenous activity declined as the cultures aged. Alternative oxidase activity could be induced in C. albicans ATCC 10231 by treatment with cyanide, but the induction of this activity required the presence of oxygen which could be replaced, at least in part, with high concentrations of potassium ferricyanide. We infer from this that the expression of the gene encoding the alternative oxidase is under the control of a redoxsensitive transcription factor.