Microwave Pretreatment of Seeds to Extract High Quality Vegetable Oil

Microwave energy is a superior alternative to several other thermal treatments. Extraction techniques are widely employed for the isolation of bioactive compounds and vegetable oils from oil seeds. Among the different and new available techniques, microwave pretreatment of seeds is a simple and desirable method for production of high quality vegetable oils. Microwave pretreatment for oil extraction has many advantages as follow: improving oil extraction yield and quality, direct extraction capability, lower energy consumption, faster processing time and reduced solvent levels compared with conventional methods. It allows also for better retention and availability of desirable nutraceuticals, such as phytosterols and tocopherols, canolol and phenolic compounds in the extracted oil such as rapeseed oil. This can be a new step to produce nutritional vegetable oils with improved shelf life because of high antioxidant content.

Accelerated Microwave Extraction of Natural Product using the Cryogrinding

Team distillation assisted by microwave extraction (SDAM) considered as accelerated technique extraction is a combination of microwave heating and steam distillation, performed at atmospheric pressure. SDAM has been compared with the same technique coupled with the cryogrinding of seeds (SDAM -CG). Isolation and concentration of volatile compounds are performed by a single stage for the extraction of essential oil from Cuminum cyminum seeds. The essential oils extracted by these two methods for 5 min were quantitatively (yield) and qualitatively (aromatic profile) no similar. These methods yield an essential oil with higher amounts of more valuable oxygenated compounds, and allow substantial savings of costs, in terms of time, energy and plant material. SDAM and SDAM-CG is a green technology and appears as a good alternative for the extraction of essential oils from aromatic plants.

The Effect of Pyridoxine and Different Levels of Nitrogen on Physiological Indices of Corn(Zea Mays L.var.sc704)

One field experiment was conducted on corn (Zea mays L.Var. SC 704) to study the effect of three different basic levels of nitrogen (90, 140and 190 Kg/ha as urea) with 0.01% and 0.02% pyridoxine pre-sowing seed soaking for 8 hours. Water-soaked seeds were treated as controled. biomass production was recorded on 45, 70 and 95 days after sowing. Total dry material (TDM), leaf area index (LAI), crop growth rate (CGR), relative growth rate (RGR) and net assimilation rate (NAR) was calculated form 45until 95 days after sowing. Yield and its components such as kernel yield, grain weight, biologic yield, harvest index and protein percentage was measured at harvest. In general, 0.02% pyridoxine and 190 Kg pure nitrogen/ha was shown gave maximum value for growth and yield parameters. N190 + 0.02 % pyridoxine enhanced seed yield and biologic yield by 57.15% and 62.98% compared to 90kg N and water – soaked treatment.

Extraction and Characterisation of Protein Fraction from Date Palm Fruit Seeds

Date palm (Phoenix dactylifera L.) seeds are waste streams which are considered a major problem to the food industry. They contain potentially useful protein (10-15% of the whole date-s weight). Global production, industrialisation and utilisation of dates are increasing steadily. The worldwide production of date palm fruit has increased from 1.8 million tons in 1961 to 6.9 million tons in 2005, thus from the global production of dates are almost 800.000 tonnes of date palm seeds are not currently used [1]. The current study was carried out to convert the date palm seeds into useful protein powder. Compositional analysis showed that the seeds were rich in protein and fat 5.64 and 8.14% respectively. We used several laboratory scale methods to extract proteins from seed to produce a high protein powder. These methods included simple acid or alkali extraction, with or without ultrafiltration and phenol trichloroacetic acid with acetone precipitation (Ph/TCA method). The highest protein content powder (68%) was obtained by Ph/TCA method with yield of material (44%) whereas; the use of just alkali extraction gave the lowest protein content of 8%, and a yield of 32%.

Unsupervised Image Segmentation Based on Fuzzy Connectedness with Sale Space Theory

In this paper, we propose an approach of unsupervised segmentation with fuzzy connectedness. Valid seeds are first specified by an unsupervised method based on scale space theory. A region is then extracted for each seed with a relative object extraction method of fuzzy connectedness. Afterwards, regions are merged according to the values between them of an introduced measure. Some theorems and propositions are also provided to show the reasonableness of the measure for doing mergence. Experiment results on a synthetic image, a color image and a large amount of MR images of our method are reported.

The Tyrosinase and Cyclooxygenase Inhibitory Activities and Cytotoxicity Screening of Tamarindus indica Seeds

The methanolic extracts from seeds of tamarind (Tamarindus indica) was prepared by Soxhlet apparatus extraction and evaluated for total phenolic content by Folin-Ciocalteu method. Then, methanolic extract was screened biological activities (In vitro) for anti-melanogenic activity by tyrosinase inhibition test, antiinflammation activity by cyclooxygenase 1 (COX-1) and cyclooxygenase 2 (COX-2) inhibition test, and cytotoxic screening test with Vero cells. The results showed that total phenolic content, which contained in extract, was contained 27.72 mg of gallic acid equivalent per g of dry weight. The ability to inhibit tyrosinase enzyme, which exerted by Tamarind seed extracts (1 mg/ml) was 52.13 ± 0.42 %. The extract was not possessed inhibitory effect to COX-1 and COX-2 enzymes and cytotoxic effect to Vero cells. The finding is concludes that tested seed extract was possessed antimelanogenic activity with non-toxic effects. However, there was not exhibited anti-inflammatory activity. Further studies include the use of advance biological models to confirm this biological activity, as well as, the isolation and characterization of the purified compounds that it was contained.

Fenugreek in the Ecological Areas of Belarus and France

On the territory of France fenugreek is spread since long on a line from the Gironde to the Italian border. In Belarus experimental cultivation has begun since 2004. Experiments with fenugreek variety Ovari 4 were conducted about time of sowing in order to study their growth, development, and evaluation of productivity in the North-east part of Belarus and Central part of France. Reaching full ripeness of seeds the plants of fenugreek in the Central part of France requires about 94-97 days. Average seeds yield of 2011-2012 is 1259 kg/ha. Plant height is about 36,8 cm. Plants were affected by aphid and in the high moist agro-climatic conditions by powdery mildew. In North-east part of Belarus plants need 86-93 days to full ripeness. Plants of fenugreek have steam about 59 cm. The average seeds yield of 2007-2009 was about 723 kg/ha. Plants were resistant to aphid and diseases.

Effect of Cement-kiln Dust Pollution on The Vegetation in The Western Mediterranean Desert of Egypt

This study investigated the ecological effects of particulate pollution from a cement factory on the vegetation in the western Mediterranean coastal desert of Egypt. Variations in vegetation, soil chemical characters, and some responses of Atriplex halimus, as a dominant species in the study area, were investigated in some sites located in different directions from the cement factory between Burg El-Arab in the east and El-Hammam in the west. The results showed an obvious decrease in vegetation diversity, in response to cement-kiln dust pollution, that accompanied by a high dominance attributed to the high contribution of Atriplex halimus. Annual species were found to be more sensitive to cement dust pollution as they all failed to persist in highly disturbed sites. It is remarkable that cover and phytomass of Atriplex halimus were increased greatly in response to cement dust pollution, and this was accompanied by a reduction in the mature seeds and leaf-area of the plant. The few seeds of the affected individuals seemed to be more fertile and attained higher germination percentages and exhibited hardening against drought stress.

Effect of Planting Density on Yield and Yield Components of Safflower Cultivars in Spring Planting

This study carried out to determine the effect of plant densities on some agronomic characteristics of four safflower cultivars in spring planting. The experiment was conducted at Yazd, Iran- using a factorial in a randomized complete block design with four replications. Cultivars were including Arak, IL, Asteria and Local and plant densities were 10, 13.3, 20 and 40 plant/m2. Number of seeds/head, number of heads/plant, HI, 1000-seed weight and seed yield significantly decreased as planting density increased. With increasing planting density, LAI, plant height, first branch height and biological yield increased. The highest seed yield was obtained in 13.3 plant/m2 (2167 kg/ha). There were significant differences between cultivars. Local cv. had higher seed yield than the other cultivars mainly due to higher heads/plant and seeds/head.

Effects of Salinity and Drought Levels in Seed Germination of Five Crop Species

The heterotrophic seedling growth can be defined as a product of two components: (1) the weight of mobilized seed reserve, and (2) conversion efficiency of utilized seed reserve to seedling tissue. The first component can be further divided into (1) initial seed weight, and (2) the fraction of seed reserve, which is mobilized. The objective of this study was the identification of the sensitive seedling growth component(s) in response to drought and salinity stresses. Two experiments were separately conducted using various salinity levels (osmotic pressure) of 0, 0.25, 0.50, 0.75, 1, 1.25 and 1.5 MPa created using NaCl as first experiment and by polyethylene glycol (drought stress) of 0, 0.2, 0.4, 0.6, 0.8, 1, 1.2 and 1.4 MPa in second experiment. Seeds of five crops species (Hordeum vulgare, Brassica napus, Zea mays, Medicago sativa and Medicago scutellata) were used in each experiment. In both experiments, seedling growth, fraction of seed reserve utilization and weight of mobilized seed reserve decreased with increasing drought and salt intensity. However, drought and salinity stresses had no effect on the conversion efficiency. It was concluded that the sensitive component of seedling growth is the weight of mobilized seed reserve.

Callusing in Stevia rebaudiana (Natural Sweetener) for Steviol Glycoside Production

Stevia rebaudiana Bertoni (natural sweetener) belongs to Asteraceae family and can be used as substitute of artificial sweeteners for diabetic patients. Conventionally, it is cultivated by seeds or stem cutting, but seed viability rate is poor. A protocol for callus induction and multiplication was developed to produce large no. of calli in short period. Surface sterilized nodal, leaf and root explants were cultured on Murashige and Skoog (MS) medium with different concentrations of plant hormone like, IBA, kinetin, NAA, 2,4-D, and NAA in combination with 2,4-D. 100% callusing was observed from leaf explants cultured on combination of NAA and 2,4-D after three weeks while with 2,4-D, only 10% callusing was observed. Calli obtained from leaf and root explants were shiny green while with nodal explants it was hard and brown. The present findings deal with induction of callusing in Stevia to achieve the rapid callus multiplication for study of steviol glycosides in callus culture.

Polyphenolic Profile and Antioxidant Activities of Nigella Sativa Seed Extracts In Vitro and In Vivo

Nigella sativa L. is an aromatic plant belonging to the family Ranunculaceae. It has been used traditionally, especially in the middle East and India, for the treatment of asthma, cough, bronchitis, headache, rheumatism, fever, influenza and eczema. Several biological activities have been reported in Nigella sativa seeds, including antioxidant. In this context we tried to estimate the antioxidant activity of various extracts prepared from Nigella sativa seeds, methanolic extract (ME), chloroformic extract (CE), hexanic extract (HE : fixed oil), ethyl acetate extract (EAE) water extract (WE). The Folin-Ciocalteu assay showed that CE and EAE contained high level of phenolic compounds 81.31 and 72.43μg GAE/mg of extract respectively. Similarly, the CE and EAE exhibited the highest DPPH radical scavenging activity, with IC50 values of 106.56μg/ml and 121.62μg/ml respectively. In addition, CE and HE showed the most scavenging activity against superoxide radical generated in the PMS-NADH-NBT system with respective IC50 values of 361.86 μg/ml and 371.80 μg/ml, which is comparable to the activity of the standard antioxidant BHT (344.59 μg/ml). Ferrous ion chelating capacity assay showed that WE, EAE and ME are the most active with 40.57, 39.70 and 22.02 mg EDTA-E/g of extract. The inhibition of linoleic acid/ß-carotene coupled oxidation was estimated by ßcarotene bleaching assay, this showed a highest relative antioxidant activity with CE and EAE (69.82% of inhibition). The antioxidant activities of the methanolic extract and the fixed oil are confirmed by an in vivo assay in mice, the daily oral administration of methanolic extract (500 and 800 mg/kg/day) and fixed oil (2 and 4 ml/kg/day) during 21 days, resulted in a significant enhancement of the blood total antioxidant capacity (measured by KRL test) and the plasmatic antioxidant capacity towards DPPH radical.

The Effectiveness of Ultrasound Treatment on the Germination Stimulation of Barley Seed and its Alpha-Amylase Activity

In the present study, the effects of ultrasound as emerging technology were investigated on germination stimulation, amount of alpha-amylase activity on dry barley seeds before steeping stage of malting process. All experiments were carried out at 20 KHz on the ultrasonic generator in 3 different ultrasonic intensities (20, 60 and 100% setting from total power of device) and time (5, 10 and 15 min) at constant temperature (30C). For determining the effects of these parameters on enzyme the Fuwa method assay based on the decreased staining value of blue starch–iodine complexes employed for measurement an activity. The results of these assays were analyzed by Qualitek4 software using the Taguchi statistical method to evaluate the factor-s effects on enzyme activity. It has been found that when malting barley is irradiated with an ultrasonic power, a stimulating effect occurs as to the enzyme activity.

Tissue Composition and Muscularity of Lamb Legs Fed with Sunflower Seeds and Vitamin E

The purpose of this study was to evaluate the tissue composition and carcass muscularity of 32 legs of Ile de France lambs fed with diets containing sunflower seeds and vitamin E, with mean body weight of 15 kg, lodged in individual pens at 15 kg and slaughtered at 32 kg of body weight. The treatments influenced (P0,05) by the treatments. The interaction of the sunflower and vitamin E was positive for bone total weights and intermuscular fat.

Effects of Ultrasonic Treatment on Germination of Synthetic Sunflower Seeds

One problem of synthetic sunflower cultivation is an erratic germination of the seeds. To improve the germination, presowing seed treatment with an ultrasound was tested. All treatments were carried out at 40 kHz frequency with the intensities of 40, 60, 80 and 100% of the ultrasonic generator total power (250 W) for the durations of 5, 10, 15 and 20 minutes. Data on seed germination percentage, seed vigor index (SVI), root and shoot lengths of seedlings were collected. The results showed that germination, SVI, root and shoot lengths of ultrasonic treated seedlings were different from the control, depending on intensity of the ultrasound. The effects of ultrasonic treatment were significant on germination, resulting in a maximum increase of 43% at 40 and 60% intensities compared to that of the control seeds. In addition, seedlings of these 2 treatments had higher SVI and longer root and shoot lengths than that of the control seedlings. All treatment durations resulted in higher germination and SVI, longer root and higher shoot lenghts of seedlings than the control. Among the duration treatments, only SVI and seedling root length were significantly different.

A Frame Work for the Development of a Suitable Method to Find Shoot Length at Maturity of Mustard Plant Using Soft Computing Model

The production of a plant can be measured in terms of seeds. The generation of seeds plays a critical role in our social and daily life. The fruit production which generates seeds, depends on the various parameters of the plant, such as shoot length, leaf number, root length, root number, etc When the plant is growing, some leaves may be lost and some new leaves may appear. It is very difficult to use the number of leaves of the tree to calculate the growth of the plant.. It is also cumbersome to measure the number of roots and length of growth of root in several time instances continuously after certain initial period of time, because roots grow deeper and deeper under ground in course of time. On the contrary, the shoot length of the tree grows in course of time which can be measured in different time instances. So the growth of the plant can be measured using the data of shoot length which are measured at different time instances after plantation. The environmental parameters like temperature, rain fall, humidity and pollution are also play some role in production of yield. The soil, crop and distance management are taken care to produce maximum amount of yields of plant. The data of the growth of shoot length of some mustard plant at the initial stage (7,14,21 & 28 days after plantation) is available from the statistical survey by a group of scientists under the supervision of Prof. Dilip De. In this paper, initial shoot length of Ken( one type of mustard plant) has been used as an initial data. The statistical models, the methods of fuzzy logic and neural network have been tested on this mustard plant and based on error analysis (calculation of average error) that model with minimum error has been selected and can be used for the assessment of shoot length at maturity. Finally, all these methods have been tested with other type of mustard plants and the particular soft computing model with the minimum error of all types has been selected for calculating the predicted data of growth of shoot length. The shoot length at the stage of maturity of all types of mustard plants has been calculated using the statistical method on the predicted data of shoot length.