Abstract: We created the tool, which combines the powerful
GENESIS (GEneral NEural SImulation System) simulation language
with the up-to-date visualisation and internet techniques. Our
solution resides in the connection between the simulation output from
GENESIS, which is converted to the data-structure suitable for
WWW browsers and VRML (Virtual Reality Modelling Language)
viewers. The selected GENESIS simulations are once exported into
the VRML code, and stored in our neurovisualisation portal
(webserver). There, the loaded models, demonstrating mainly the
spread of electrical signal (action potentials, postsynaptic potentials)
along the neuronal membrane (axon, dendritic tree, neuron) could be
displayed in the client-s VRML viewer, without interacting with
original GENESIS environment. This enables the visualisation of
basic neurophysiological phenomena designed for GENESIS
simulator on the independent OS (operation system).
Abstract: Attachment of the circulating monocytes to the
endothelium is the earliest detectable events during formation of
atherosclerosis. The adhesion molecules, chemokines and matrix
proteases genes were identified to be expressed in atherogenesis.
Expressions of these genes may influence structural integrity of the
luminal endothelium. The aim of this study is to relate changes in the
ultrastructural morphology of the aortic luminal surface and gene
expressions of the endothelial surface, chemokine and MMP-12 in
normal and hypercholesterolemic rabbits. Luminal endothelial
surface from rabbit aortic tissue was examined by scanning electron
microscopy (SEM) using low vacuum mode to ascertain
ultrastructural changes in development of atherosclerotic lesion. Gene
expression of adhesion molecules, MCP-1 and MMP-12 were studied
by Real-time PCR. Ultrastructural observations of the aortic luminal
surface exhibited changes from normal regular smooth intact
endothelium to irregular luminal surface including marked globular
appearance and ruptures of the membrane layer. Real-time PCR
demonstrated differentially expressed of studied genes in
atherosclerotic tissues. The appearance of ultrastructural changes in
aortic tissue of hypercholesterolemic rabbits is suggested to have
relation with underlying changes of endothelial surface molecules,
chemokine and MMP-12 gene expressions.
Abstract: A new supported liquid membrane (SLM) system for
the selective transport of VO2
+ ions was prepared in this present
work. The SLM was a thin porous polyvinylidene difluoride
(PVDF) membrane soaked with Di-(2-ethylhexyl) phosphoric acid
(D2EHPA) as mobile carrier in Xylene as organic solvent.
D2EHPA acts as a highly selective carrier for the uphill transport of
VO2
+ ions through the SLM. The transport of VO2
+ ions reached to
64%. In the presence of P2O7-2 ion as suitable masking agent in the
feed solution, the interfering effects of other cations were eliminated.
Abstract: This paper details few mechanical modeling and
design issues of RF MEMS switches. We concentrate on an
electrostatically actuated broad side series switch; surface
micromachined with a crab leg membrane. The same results are
extended to any complex structure. With available experimental data
and fabrication results, we present the variation in dynamic
performance and compliance of the switch with reference to few
design issues, which we find are critical in deciding the dynamic
behavior of the switch, without compromise on the RF
characteristics. The optimization of pull in voltage, transient time and
resonant frequency with regard to these critical design parameters are
also presented.
Abstract: Microorganisms isolated from water and soil of
Kazakhstan to identify potential high-effective producers of the
arachidonic acid, exhibiting a wide range of physiological activity
and having practical applications were screened. Based on the results
of two independent tests (the test on the sensitivity of the growth
processes of microorganisms to acetylsalicylic acid - an irreversible
inhibitor of PGH-synthase involved in the metabolism of arachidonic
acid and its derivatives, the test for inhibition of peroxidase activity
of membrane-bounding fraction of PGH - synthase by acetylsalicylic
acid) were selected microbial cultures which are potential highproducer
of arachidonic acid. They are characterized by a stable
strong growth in the laboratory conditions. Identification of
microorganism cultures based on morphological, physiological,
biochemical and molecular genetic characteristics was performed.
Abstract: In this work Membrane Distillation is applied to
concentrate orange Juice. Clarified orange juice (11o Brix) obtained
from fresh fruits and a sugar solution was subjected to membrane
distillation. The experiments were performed on a flat sheet module
using orange juice and sucrose solution as feeds. The concentration
of a sucrose solution, used as a model fruit juice and also orange
juice, was carried out in a direct contact membrane distillation using
hydrophobic PTFE membrane of pore size 0.2 μm and porosity 70%.
Surface modification of PTFE membrane has been carried out by
treating membrane with alcohol and water solution to make it
hydrophilic and then hydrophobicity was regained by drying. The
influences of the feed temperature, feed concentration, flow rate,
operating time on the permeate flux were studied for treated and non
treated membrane. In this work treated and non treated membrane
were compared in terms of water flux, Within the tested range, MD
with surface modified membrane the water flux has been
significantly improved by treating the membrane surface.
Abstract: The choice of finite element to use in order to predict
nonlinear static or dynamic response of complex structures becomes
an important factor. Then, the main goal of this research work is to
focus a study on the effect of the in-plane rotational degrees of
freedom in linear and geometrically non linear static and dynamic
analysis of thin shell structures by flat shell finite elements. In this
purpose: First, simple triangular and quadrilateral flat shell finite
elements are implemented in an incremental formulation based on the
updated lagrangian corotational description for geometrically
nonlinear analysis. The triangular element is a combination of DKT
and CST elements, while the quadrilateral is a combination of DKQ
and the bilinear quadrilateral membrane element. In both elements,
the sixth degree of freedom is handled via introducing fictitious
stiffness. Secondly, in the same code, the sixth degrees of freedom in
these elements is handled differently where the in-plane rotational
d.o.f is considered as an effective d.o.f in the in-plane filed
interpolation. Our goal is to compare resulting shell elements. Third,
the analysis is enlarged to dynamic linear analysis by direct
integration using Newmark-s implicit method. Finally, the linear
dynamic analysis is extended to geometrically nonlinear dynamic
analysis where Newmark-s method is used to integrate equations of
motion and the Newton-Raphson method is employed for iterating
within each time step increment until equilibrium is achieved. The
obtained results demonstrate the effectiveness and robustness of the
interpolation of the in-plane rotational d.o.f. and present deficiencies
of using fictitious stiffness in dynamic linear and nonlinear analysis.
Abstract: In a world worried about water resources with the
shadow of drought and famine looming all around, the quality of
water is as important as its quantity. The source of all concerns is the
constant reduction of per capita quality water for different uses.
Iran With an average annual precipitation of 250 mm compared to
the 800 mm world average, Iran is considered a water scarce country
and the disparity in the rainfall distribution, the limitations of
renewable resources and the population concentration in the margins
of desert and water scarce areas have intensified the problem.
The shortage of per capita renewable freshwater and its poor
quality in large areas of the country, which have saline, brackish or
hard water resources, and the profusion of natural and artificial
pollutant have caused the deterioration of water quality.
Among methods of treatment and use of these waters one can refer
to the application of membrane technologies, which have come into
focus in recent years due to their great advantages. This process is
quite efficient in eliminating multi-capacity ions; and due to the
possibilities of production at different capacities, application as
treatment process in points of use, and the need for less energy in
comparison to Reverse Osmosis processes, it can revolutionize the
water and wastewater sector in years to come. The article studied the
different capacities of water resources in the Persian Gulf and Oman
Sea watershed basins, and processes the possibility of using
nanofiltration process to treat brackish and non-conventional waters
in these basins.
Abstract: In this study, the designed dual stage membrane
bioreactor (MBR) system was conceptualized for the treatment of
cyanide and heavy metals in electroplating wastewater. The design
consisted of a primary treatment stage to reduce the impact of
fluctuations and the secondary treatment stage to remove the residual
cyanide and heavy metal contaminants in the wastewater under
alkaline pH conditions. The primary treatment stage contained
hydrolyzed Citrus sinensis (C. sinensis) pomace and the secondary
treatment stage contained active Aspergillus awamori (A. awamori)
biomass, supplemented solely with C. sinensis pomace extract from
the hydrolysis process. An average of 76.37%, 95.37%, 93.26 and
94.76% and 99.55%, 99.91%, 99.92% and 99.92% degradation
efficiency for total cyanide (T-CN), including the sorption of nickel
(Ni), zinc (Zn) and copper (Cu) were observed after the first and
second treatment stages, respectively. Furthermore, cyanide
conversion by-products degradation was 99.81% and 99.75 for both
formate (CHOO-) and ammonium (NH4
+) after the second treatment
stage. After the first, second and third regeneration cycles of the C.
sinensis pomace in the first treatment stage, Ni, Zn and Cu removal
achieved was 99.13%, 99.12% and 99.04% (first regeneration cycle),
98.94%, 98.92% and 98.41% (second regeneration cycle) and 98.46
%, 98.44% and 97.91% (third regeneration cycle), respectively.
There was relatively insignificant standard deviation detected in all
the measured parameters in the system which indicated
reproducibility of the remediation efficiency in this continuous
system.
Abstract: This paper proposes the concept of aerocapture with
aerodynamic-environment-adaptive variable geometry flexible
aeroshell that vehicle deploys. The flexible membrane is composed
of thin-layer film or textile as its aeroshell in order to solve some
problems obstructing realization of aerocapture technique.
Multi-objective optimization study is conducted to investigate
solutions and derive design guidelines. As a result, solutions which
can avoid aerodynamic heating and enlarge the corridor width up
to 10% are obtained successfully, so that the effectiveness of this
concept can be demonstrated. The deformation-use optimum
solution changes its drag coefficient from 1.6 to 1.1, along with the
change in dynamic pressure. Moreover, optimization results show
that deformation-use solution requires the membrane for which
upper temperature limit and strain limit are more than 700 K and
120%, respectively, and elasticity (Young-s modulus) is of order of
106 Pa.
Abstract: Bones are dynamic and responsive organs, they
regulate their strength and mass according to the loads which they are subjected. Because, the Wnt/β-catenin pathway has profound
effects on the regulation of bone mass, we hypothesized that mechanical loading of bone cells stimulates Wnt/β-catenin signaling, which results in the generation of new bone mass.
Mechanical loading triggers the secretion of the Wnt molecule, which after binding to transmembrane proteins, causes GSK-3β (Glycogen synthase kinase 3 beta) to cease the phosphorylation of β-catenin. β-catenin accumulation in the cytoplasm, followed by its
transport into the nucleus, binding to transcription factors (TCF/LEF)
that initiate transcription of genes related to bone formation. To test this hypothesis, we used TOPGAL (Tcf Optimal Promoter
β-galactosidase) mice in an experiment in which cyclic loads were
applied to the forearm. TOPGAL mice are reporters for cells effected
by the Wnt/β-catenin signaling pathway. TOPGAL mice are genetically engineered mice in which transcriptional activation of β-
catenin, results in the production of an enzyme, β-galactosidase. The
presence of this enzyme allows us to localize transcriptional
activation of β-catenin to individual cells, thereby, allowing us to quantify the effects that mechanical loading has on the Wnt/β-catenin pathway and new bone formation. The ulnae of loaded TOPGAL
mice were excised and transverse slices along different parts of the
ulnar shaft were assayed for the presence of β-galactosidase.
Our results indicate that loading increases β-catenin transcriptional
activity in regions where this pathway is already primed (i.e. where basal activity is already higher) in a load magnitude dependent
manner. Further experiments are needed to determine the temporal and spatial activation of this signaling in relation to bone formation.
Abstract: PDMS (Polydimethylsiloxane) polymer is a suitable material for biological and MEMS (Microelectromechanical systems) designers, because of its biocompatibility, transparency and high resistance under plasma treatment. PDMS round channel is always been of great interest due to its ability to confine the liquid with membrane type micro valves. In this paper we are presenting a very simple way to form round shapemicrofluidic channel, which is based on reflow of positive photoresist AZ® 40 XT. With this method, it is possible to obtain channel of different height simply by varying the spin coating parameters of photoresist.
Abstract: Aptamers are useful tools in microorganism
researches, diagnoses, and treatment. Aptamers are specific target
molecules formed by oligonucleic acid molecules, and are not
decomposed by alcohol. Aptamers used to detect Mycobacterium
tuberculosis (MTB) have been proved to have specific affinity to the
outer membrane proteins of MTB. This article presents a biosensor
chip set with aptamers for early detection of MTB with high specificity
and sensitivity, even in very low concentration. Meanwhile, we have
already made a modified hydrophobic facial mask module with
internal rendering hydrophobic for effectively collecting M.
tuberculosis.
Abstract: Fuel and oxidant gas delivery plate, or fuel cell
plate, is a key component of a Proton Exchange Membrane (PEM)
fuel cell. To manufacture low-cost and high performance fuel cell
plates, advanced computer modeling and finite element structure
analysis are used as virtual prototyping tools for the optimization
of the plates at the early design stage. The present study examines
thermal stress analysis of the fuel cell plates that are produced
using a patented, low-cost fuel cell plate production technique
based on screen-printing. Design optimization is applied to
minimize the maximum stress within the plate, subject to strain
constraint with both geometry and material parameters as design
variables. The study reveals the characteristics of the printed
plates, and provides guidelines for the structure and material design
of the fuel cell plate.
Abstract: In this work, the plate bending formulation of the boundary element method - BEM, based on the Reissner?s hypothesis, is extended to the analysis of plates reinforced by beams taking into account the membrane effects. The formulation is derived by assuming a zoned body where each sub-region defines a beam or a slab and all of them are represented by a chosen reference surface. Equilibrium and compatibility conditions are automatically imposed by the integral equations, which treat this composed structure as a single body. In order to reduce the number of degrees of freedom, the problem values defined on the interfaces are written in terms of their values on the beam axis. Initially are derived separated equations for the bending and stretching problems, but in the final system of equations the two problems are coupled and can not be treated separately. Finally are presented some numerical examples whose analytical results are known to show the accuracy of the proposed model.
Abstract: In the first part of the research work, an electrolyzer (10.16 cm dia and 24.13 cm height) to produce hydrogen and oxygen was constructed for single slice O2/H2 fuel cell using cation exchange membrane. The electrolyzer performance was tested with 23% NaOH, 30% NaOH, 30% KOH and 35% KOH electrolyte solution with current input 4 amp and 2.84 V from the rectifier. Rates of volume of hydrogen produced were 0.159 cm3/sec, 0.155 cm3/sec, 0.169 cm3/sec and 0.163 cm3/sec respectively from 23% NaOH, 30% NaOH, 30% KOH and 35% KOH solution. Rates of volume of oxygen produced were 0.212 cm3/sec, 0.201 cm3/sec, 0.227 cm3/sec and 0.219 cm3/sec respectively from 23% NaOH, 30% NaOH, 30% KOH and 35% KOH solution (1.5 L). In spite of being tested the increased concentration of electrolyte solution, the gas rate does not change significantly. Therefore, inexpensive 23% NaOH electrolyte solution was chosen to use as the electrolyte in the electrolyzer. In the second part of the research work, graphite serpentine flow plates, fiberglass end plates, stainless steel screen electrodes, silicone rubbers were made to assemble the single slice O2/H2 polymer electrolyte membrane fuel cell (PEMFC).
Abstract: Application of nanoparticles as additives in membrane
synthesis for improving the resistance of membranes against fouling
has triggered recent interest in new membrane types. However, most
nanoparticle-enhanced membranes suffer from the tradeoff between
permeability and selectivity. In this study, nano-WS2 was explored as
the additive in membrane synthesis by non-solvent induced phase
separation. Blended PES-WS2 flat-sheet membranes with the
incorporation of ultra-low concentrations of nanoparticles (from 0.025
to 0.25%, WS2/PES ratio) were manufactured and investigated in
terms of permeability, fouling resistance and solute rejection.
Remarkably, a significant enhancement in the permeability was
observed as a result of the incorporation of ultra-low fractions of
nano-WS2 to the membrane structure. Optimal permeability values
were obtained for modified membranes with 0.10%
nanoparticle/polymer concentration ratios. Furthermore, fouling
resistance and solute rejection were significantly improved by the
incorporation of nanoparticles into the membrane matrix. Specifically,
fouling resistance of modified membrane can increase by around 50%.
Abstract: Liposomal magnetofection is the most powerful nonviral method for the nucleic acid delivery into the cultured cancer cells and widely used for in vitro applications. Use of the static magnetic field condition may result in non-uniform distribution of aggregate complexes on the surface of cultured cells. To prevent this, we developed the new device which allows to concentrate aggregate complexes under dynamic magnetic field, assisting more contact of these complexes with cellular membrane and, possibly, stimulating endocytosis. Newly developed device for magnetofection under dynamic gradient magnetic field, “DynaFECTOR", was used to compare transfection efficiency of human liver hepatocellular carcinoma cell line HepG2 with that obtained by lipofection and magnetofection. The effect of two parameters on transfection efficiency, incubation time under dynamic magnetic field and rotation frequency of magnet, was estimated. Liposomal magnetofection under dynamic gradient magnetic field showed the highest transfection efficiency for HepG2 cells.
Abstract: This study presents the performance of membrane
bioreactor in treating high phosphate wastewater. The laboratory
scale MBR was operated at permeate flux of 25 L/m2.h with a hollow
fiber membrane (polypropylene, approx. pore size 0.01 - 0.2 μm) at
hydraulic retention time (HRT) of 12 hrs. Scanning electron
microscopy (SEM) and energy diffusive X-ray (EDX) analyzer were
used to characterize the membrane foulants. Results showed that the
removal efficiencies of COD, TSS, NH3-N and PO4
3- were 93, 98, 80
and 30% respectively. On average 91% of influent soluble microbial
products (SMP) were eliminated, with the eliminations of
polysaccharides mostly above 80%. The main fouling resistance was
cake resistance. It should be noted that SMP were found in major
portions of mixed liquor that played a relatively significant role in
membrane fouling. SEM and EDX analyses indicated that the
foulants covering the membrane surfaces comprises not only organic
substances but also inorganic elements including Mg, Ca, Al, K and
P.