Screening and Identification of Microorganisms – Potential Producers of Arachidonic Acid
Microorganisms isolated from water and soil of
Kazakhstan to identify potential high-effective producers of the
arachidonic acid, exhibiting a wide range of physiological activity
and having practical applications were screened. Based on the results
of two independent tests (the test on the sensitivity of the growth
processes of microorganisms to acetylsalicylic acid - an irreversible
inhibitor of PGH-synthase involved in the metabolism of arachidonic
acid and its derivatives, the test for inhibition of peroxidase activity
of membrane-bounding fraction of PGH - synthase by acetylsalicylic
acid) were selected microbial cultures which are potential highproducer
of arachidonic acid. They are characterized by a stable
strong growth in the laboratory conditions. Identification of
microorganism cultures based on morphological, physiological,
biochemical and molecular genetic characteristics was performed.
<p>[1] A. R. Brash, “Arachidonic acid as a bioactive molecule” J. of Clinical
Investigation, vol.107, no. 11, pp. 1339-1345, 2001.
[2] M. G. Sergeeva, A. T. Varfolomeeva, The cascade of arachidonic acid.
Moscow: Public education, 2006, 256 p.
[3] S. H. Mitmesser, C. L. Jensen, “Roles of long-chain polyunsaturated
fatty acids in the term infant: developmental benefits”, Neonatal
Network, vol. 26, no. 4, pp. 229-234, 2007.
[4] G. Bernardi, New comprehensive biochemistry: Biochemistry of lipids,
lipoproteins and membranes, Amsterdam: Elsevier, 1996, pp. 141–152.
[5] E. G. Deduhina, T. I. Chistyakova, M. B. Vainshtein, “Biosynthesis of
arachidonic acid by Micromycetes” (review), Applied biochemistry and
microbiology, vol. 47, no. 2, pp. 125-134, 2011.
[6] K. Higashiyama, S. Fujikawa, E. Y. Park, S. Shimizu, “Production of
Arachidonic Acid by Mortierella Fungi”, Biotechnology and Bioprocess
Engineering, vol. 7, pp. 252-262, 2002.
[7] Edited by A. N. Netrusova М. Workshop on Microbiology, Academia,
2005, pp. 448- 597.
[8] E. Z. Tepper, V. K. Shilnikova, Workshop on Microbiology, Мoscow:
Drofa, 2004, 256 p.
[9] Edited by N.S Yegorov, Guide to practical training in Microbiology:
Textbook, 3rd ed., Moscow: Moscow State University Press, 1995,
224p.
[10] V. K. Eroshin, E. G. Dedyukhina, T. I. Chistyakova, V. P. Zhelifonova,
C. P. Kurtzman, R. J. Bothast, “Arachidonic-acid production by species
of Mortierella”, World Journal of Microbiology & Biotechnology, vol.
12, no. 1, pp. 91-96, 1996.
[11] J. G. Holt, Noel. R. Krieg, Peter H. A. Sneath, James T. Staley, Stanley
T. Williams, Bergey's Manual of Determinative Bacteriology”, 9-th. Ed,
vol.1-2, 1997.
[12] I. N. Scvortcova, Identification of soil bacteria of the Bacillus, Part 1,
Мoscow: MSU, 1983, 43 p.
[13] O. A. Nesterenko, E. N. Kvasnikov, T. M. Nogina, Nocardio and corinesimilar
bacteria, Kiev, 1985, 331 p.
[14] K. Wilson, “Preparation of genomic DNA from bacteria”, Current
Protocols in Molecular Biology, (F. M. Ausubel, R. Brent, R. E.
Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, et al.), New York:
Wiley, 1987, 650 p.
[15] R. A. Clayton, G. Sutton, P. S. Hinkle, Jr. C. Bult, C. Fields,
“Intraspecific variation in small-subunit rRNA sequences in GenBank:
why single sequences may not adequately represent prokaryotic taxa”
International Journal of Systematic Bacteriology, vol. 45, pp. 595-599,
1995.
[16] E. Werle, C. Schneider, M. Renner, M. Völker, W. Fiehn, “Convenient
single-step, one tube purification of PCR products for direct
sequencing”, Nucleic Acids Research, vol. 22, pp. 4354-4355, 1994.
[17] H. Maciel, C. Gouvêa, M. Toyama, M. Smolka, S. Marangoni, G.
Pastore, “Еxtraction, purification and biochemical characterization of a
peroxidase from Copaifera langsdorffii leaves”, Química Nova, vol. 30,
no. 5, pp. 1067-1071, 2007.
[18] O. H. Lowry, N. J. Rosebrough, A. L. Farr, R. J. Randall, “Protein
measurement with Folin phenol reagent”, Journal of Biological
Chemistry, vol.193, no 1, pp. 265-275,1951.
[19] P. I. Terentyev, N. S. Rostova, Workshop on Biometrics, Leningrad: SP.
U., 1977, 234 p.
[20] J. R. Vane, R. M. Botting, “The mechanism of action of aspirin”,
Thrombosis Research, no. 110, pp. 255–258, 2003.</p>
<p>[1] A. R. Brash, “Arachidonic acid as a bioactive molecule” J. of Clinical
Investigation, vol.107, no. 11, pp. 1339-1345, 2001.
[2] M. G. Sergeeva, A. T. Varfolomeeva, The cascade of arachidonic acid.
Moscow: Public education, 2006, 256 p.
[3] S. H. Mitmesser, C. L. Jensen, “Roles of long-chain polyunsaturated
fatty acids in the term infant: developmental benefits”, Neonatal
Network, vol. 26, no. 4, pp. 229-234, 2007.
[4] G. Bernardi, New comprehensive biochemistry: Biochemistry of lipids,
lipoproteins and membranes, Amsterdam: Elsevier, 1996, pp. 141–152.
[5] E. G. Deduhina, T. I. Chistyakova, M. B. Vainshtein, “Biosynthesis of
arachidonic acid by Micromycetes” (review), Applied biochemistry and
microbiology, vol. 47, no. 2, pp. 125-134, 2011.
[6] K. Higashiyama, S. Fujikawa, E. Y. Park, S. Shimizu, “Production of
Arachidonic Acid by Mortierella Fungi”, Biotechnology and Bioprocess
Engineering, vol. 7, pp. 252-262, 2002.
[7] Edited by A. N. Netrusova М. Workshop on Microbiology, Academia,
2005, pp. 448- 597.
[8] E. Z. Tepper, V. K. Shilnikova, Workshop on Microbiology, Мoscow:
Drofa, 2004, 256 p.
[9] Edited by N.S Yegorov, Guide to practical training in Microbiology:
Textbook, 3rd ed., Moscow: Moscow State University Press, 1995,
224p.
[10] V. K. Eroshin, E. G. Dedyukhina, T. I. Chistyakova, V. P. Zhelifonova,
C. P. Kurtzman, R. J. Bothast, “Arachidonic-acid production by species
of Mortierella”, World Journal of Microbiology & Biotechnology, vol.
12, no. 1, pp. 91-96, 1996.
[11] J. G. Holt, Noel. R. Krieg, Peter H. A. Sneath, James T. Staley, Stanley
T. Williams, Bergey's Manual of Determinative Bacteriology”, 9-th. Ed,
vol.1-2, 1997.
[12] I. N. Scvortcova, Identification of soil bacteria of the Bacillus, Part 1,
Мoscow: MSU, 1983, 43 p.
[13] O. A. Nesterenko, E. N. Kvasnikov, T. M. Nogina, Nocardio and corinesimilar
bacteria, Kiev, 1985, 331 p.
[14] K. Wilson, “Preparation of genomic DNA from bacteria”, Current
Protocols in Molecular Biology, (F. M. Ausubel, R. Brent, R. E.
Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, et al.), New York:
Wiley, 1987, 650 p.
[15] R. A. Clayton, G. Sutton, P. S. Hinkle, Jr. C. Bult, C. Fields,
“Intraspecific variation in small-subunit rRNA sequences in GenBank:
why single sequences may not adequately represent prokaryotic taxa”
International Journal of Systematic Bacteriology, vol. 45, pp. 595-599,
1995.
[16] E. Werle, C. Schneider, M. Renner, M. Völker, W. Fiehn, “Convenient
single-step, one tube purification of PCR products for direct
sequencing”, Nucleic Acids Research, vol. 22, pp. 4354-4355, 1994.
[17] H. Maciel, C. Gouvêa, M. Toyama, M. Smolka, S. Marangoni, G.
Pastore, “Еxtraction, purification and biochemical characterization of a
peroxidase from Copaifera langsdorffii leaves”, Química Nova, vol. 30,
no. 5, pp. 1067-1071, 2007.
[18] O. H. Lowry, N. J. Rosebrough, A. L. Farr, R. J. Randall, “Protein
measurement with Folin phenol reagent”, Journal of Biological
Chemistry, vol.193, no 1, pp. 265-275,1951.
[19] P. I. Terentyev, N. S. Rostova, Workshop on Biometrics, Leningrad: SP.
U., 1977, 234 p.
[20] J. R. Vane, R. M. Botting, “The mechanism of action of aspirin”,
Thrombosis Research, no. 110, pp. 255–258, 2003.</p>
@article{"International Journal of Biological, Life and Agricultural Sciences:50561", author = "A. V. Goncharova and T. A. Karpenyuk and Y. S. Tsurkan and R. U. Beisembaeva and A. M. Kalbaeva and T. D. Mukasheva and L. V. Ignatova", title = "Screening and Identification of Microorganisms – Potential Producers of Arachidonic Acid", abstract = "Microorganisms isolated from water and soil of
Kazakhstan to identify potential high-effective producers of the
arachidonic acid, exhibiting a wide range of physiological activity
and having practical applications were screened. Based on the results
of two independent tests (the test on the sensitivity of the growth
processes of microorganisms to acetylsalicylic acid - an irreversible
inhibitor of PGH-synthase involved in the metabolism of arachidonic
acid and its derivatives, the test for inhibition of peroxidase activity
of membrane-bounding fraction of PGH - synthase by acetylsalicylic
acid) were selected microbial cultures which are potential highproducer
of arachidonic acid. They are characterized by a stable
strong growth in the laboratory conditions. Identification of
microorganism cultures based on morphological, physiological,
biochemical and molecular genetic characteristics was performed.
", keywords = "Arachidonic acid, aspirin-sensitive culture, bacteria,
producers, screening.", volume = "7", number = "7", pages = "494-4", }
