Abstract: In this paper, a direct method based on variable step
size Block Backward Differentiation Formula which is referred as
BBDF2 for solving second order Ordinary Differential Equations
(ODEs) is developed. The advantages of the BBDF2 method over the
corresponding sequential variable step variable order Backward
Differentiation Formula (BDFVS) when used to solve the same
problem as a first order system are pointed out. Numerical results are
given to validate the method.
Abstract: A parallel block method based on Backward
Differentiation Formulas (BDF) is developed for the parallel solution
of stiff Ordinary Differential Equations (ODEs). Most common
methods for solving stiff systems of ODEs are based on implicit
formulae and solved using Newton iteration which requires repeated
solution of systems of linear equations with coefficient matrix, I -
hβJ . Here, J is the Jacobian matrix of the problem. In this paper,
the matrix operations is paralleled in order to reduce the cost of the
iterations. Numerical results are given to compare the speedup and
efficiency of parallel algorithm and that of sequential algorithm.
Abstract: Ethanol is generally used as a therapeutic reagent against Hepatocellular carcinoma (HCC or hepatoma) worldwide, as it can induce Hepatocellular carcinoma cell apoptosis at low concentration through a multifactorial process regulated by several unknown proteins. This paper provides a simple and available proteomic strategy for exploring differentially expressed proteins in the apoptotic pathway. The appropriate concentrations of ethanol required to induce HepG2 cell apoptosis were first assessed by MTT assay, Gisma and fluorescence staining. Next, the central proteins involved in the apoptosis pathway processs were determined using 2D-PAGE, SDS-PAGE, and bio-software analysis. Finally the downregulation of two proteins, AFP and survivin, were determined by immunocytochemistry and reverse transcriptase PCR (RT-PCR) technology. The simple, useful method demonstrated here provides a new approach to proteomic analysis in key bio-regulating process including proliferation, differentiation, apoptosis, immunity and metastasis.
Abstract: The implicit block methods based on the backward
differentiation formulae (BDF) for the solution of stiff initial value
problems (IVPs) using variable step size is derived. We construct a
variable step size block methods which will store all the coefficients
of the method with a simplified strategy in controlling the step size
with the intention of optimizing the performance in terms of
precision and computation time. The strategy involves constant,
halving or increasing the step size by 1.9 times the previous step size.
Decision of changing the step size is determined by the local
truncation error (LTE). Numerical results are provided to support the
enhancement of method applied.
Abstract: IEEE 802.11e is the enhanced version of the IEEE
802.11 MAC dedicated to provide Quality of Service of wireless
network. It supports QoS by the service differentiation and
prioritization mechanism. Data traffic receives different priority
based on QoS requirements. Fundamentally, applications are divided
into four Access Categories (AC). Each AC has its own buffer queue
and behaves as an independent backoff entity. Every frame with a
specific priority of data traffic is assigned to one of these access
categories. IEEE 802.11e EDCA (Enhanced Distributed Channel
Access) is designed to enhance the IEEE 802.11 DCF (Distributed
Coordination Function) mechanisms by providing a distributed
access method that can support service differentiation among
different classes of traffic. Performance of IEEE 802.11e MAC layer
with different ACs is evaluated to understand the actual benefits
deriving from the MAC enhancements.
Abstract: Bone marrow-derived stem cells have been widely
studied as an alternative source of stem cells. Mesenchymal stem
cells (MSCs) were mostly investigated and studies showed MSCs can
promote neurogenesis. Little is known about the non-mesenchymal
mononuclear cell fraction, which contains both hematopoietic and
nonhematopoietic cells, including monocytes and endothelial
progenitor cells. This study focused on unfractionated bone marrow
mononuclear cells (BMMCs), which remained 72 h after MSCs were
adhered to the culture plates. We showed that BMMC-conditioned
medium promoted morphological changes of human SH-SY5Y
neuroblastoma cells from an epithelial-like phenotype towards a
neuron-like phenotype as indicated by an increase in neurite
outgrowth, like those observed in retinoic acid (RA)-treated cells.
The result could be explained by the effects of trophic factors
released from BMMCs, as shown in the RT-PCR results that
BMMCs expressed nerve growth factor (NGF), brain-derived
neurotrophic factor (BDNF), and ciliary neurotrophic factor (CNTF).
Similar results on the cell proliferation rate were also observed
between RA-treated cells and cells cultured in BMMC-conditioned
medium, suggesting that cells creased proliferating and differentiated
into a neuronal phenotype. Using real-time RT-PCR, a significantly
increased expression of tyrosine hydroxylase (TH) mRNA in SHSY5Y
cells indicated that BMMC-conditioned medium induced
catecholaminergic identities in differentiated SH-SY5Y cells.
Abstract: The main objective of this study was to demonstrate that differentiation of infected and vaccinated animals (DIVA) strategy using different ELISA tests is possible when a subunit vaccine (Haemagglutinin protein) is used to prevent Avian influenza. Special emphasis was placed on the differentiation in the serological response to different components of the AIV (Nucleoprotein, Neuraminidase, Haemagglutinin, Nucleocapsid) between chickens that were vaccinated with a whole virus kill vaccine and recombinant vaccine. Furthermore, the potential use of this DIVA strategy using ELISA assays to detect Neuraminidase 1 (N1) was analyzed as strategy in countries where the field virus is H5N1 and the vaccine used is formulated with H5N2. Detection of AIV-s antibodies to any component in serum was negative for all animals on the study days 0-13. At study day 14 the titers of antibodies against Nucleoprotein (NP) and Nucleocapsid (NC) rose in the experimental groups vaccinated with Volvac® AI KV and were negatives during all the trial in the experimental groups vaccinated with a subunit H5; significant statistically differences were observed between these groups (p < 0.05). The seroconversion either Haemagglutinin or Neuraminidase was evident after 21 days post-vaccination in the experimental groups vaccinated with the respective viral fraction. Regarding the main aim of this study and according with the results that were obtained, use a combination of different ELISA test as a DIVA strategy is feasible when the vaccination is carry out with a subunit H5 vaccine. Also is possible to use the ELISA kit to detect Neuraminidase (either N1 or N2) as a DIVA concept in countries where H5N1 is present and the vaccination programs are done with H5N2 vaccine.
Abstract: Processes of plant breeding, testing and licensing of new varieties, patent protection in seed production, relations in trade and protection of copyright are dependent on identification, differentiation and characterization of plant genotypes. Therefore, we focused our research on utilization of wheat storage proteins as genetic markers suitable not only for differentiation of individual genotypes, but also for identification and characterization of their considerable properties. We analyzed a collection of 102 genotypes of bread wheat (Triticum aestivum L.), 41 genotypes of spelt wheat (Triticum spelta L.), and 35 genotypes of durum wheat (Triticum durum Desf.), in this study. Our results show, that genotypes of bread wheat and durum wheat were homogenous and single line, but spelt wheat genotypes were heterogenous. We observed variability of HMW-GS composition according to environmental factors and level of breeding and predict technological quality on the basis of Glu-score calculation.
Abstract: Protein and Esterase electrophoresis were used to
genetically identify two Saudi tick species. Engorged females of the
camel tick Hyalomma dromedarii (Koch) (Acari: Ixodidae) and the
cattle tick Boophilus annulatus (Say) (Acari: Ixodidae) ticks
collected from infested camels and cattle in the animals resting
house at Hail region in KSA were used. The results showed that
there are a variation in both of protein and esterase activity levels and
a high polymorphism within and between the genera and species of
Hyalomma and Boophilus . In conclusion, the protein and esterase
electrophoretic analysis used in the present study could successfully
distinguish among tick species, commonly found in Saudi Arabia.
Abstract: The main objective of our study is to collect data
about the profile of the asthmatic patients in Assam and thereby have
a comprehensive knowledge of the factors influencing the asthmatic
patients of the state and their medication pattern. We developed a
search strategy to find any publication about the community based
survey asthma related and used. These to search the MEDLINE
(1996 to current literature) CINAHL DOAJ pubmed databases using
the key phrases, Asthma, Respiratory disorders, Drug therapy of
Asthma, database decision support system and asthma. The
appropriate literature was printed out from the online source and
library (Journal) source. The study was conducted through a set of
structured and non-structured questionnaires targeted on the
asthmatic patients belonging to the rural and urban areas of Assam,
during the month of Dec 2006 to July 2007, 138 cases were studied
in Gauwathi Medical College & Hospital located in Bhangagarh,
Assam in India. The demographic characteristics a factor in 138
patients with asthma with allergic rhinitis (cases) gives the detail
profile of asthmatic patient-s distribution of Assam as classified on
the basis of age and sex. It is evident from the study that male
populations (66%) are more prone to asthma as compared to the
females (34%).Another striking features that emerged from this
survey is the maximum prevalence of asthma in the age group of 20-
30 years followed by infants belonging to the age group of 7 (0.05%)
0-10years among both male and female populations of Assam. The
high incidence of asthma in the age group of 20-30 years may
probably be due to the allergy arising out of sudden exposure to dust
and pollen which the children face while playing and going to the
school. The rural females in the age group of 30-40 years are more
prone to asthma than urban females in the same age group may be
due to sex differentiation among the tribal population of the state.
Pharmacists should educate the asthmatics how to use inhalers
considering growing menace of asthma in the state. Safer drugs
should be produced in the form of aerosol so that easy administration
by the asthmatic patients and physicians of the state is possible for
curing asthma. The health centers should be more equipped with the
medicines to cure asthma in the state like Assam.
Abstract: Reduction of Single Input Single Output (SISO) discrete systems into lower order model, using a conventional and an evolutionary technique is presented in this paper. In the conventional technique, the mixed advantages of Modified Cauer Form (MCF) and differentiation are used. In this method the original discrete system is, first, converted into equivalent continuous system by applying bilinear transformation. The denominator of the equivalent continuous system and its reciprocal are differentiated successively, the reduced denominator of the desired order is obtained by combining the differentiated polynomials. The numerator is obtained by matching the quotients of MCF. The reduced continuous system is converted back into discrete system using inverse bilinear transformation. In the evolutionary technique method, Particle Swarm Optimization (PSO) is employed to reduce the higher order model. PSO method is based on the minimization of the Integral Squared Error (ISE) between the transient responses of original higher order model and the reduced order model pertaining to a unit step input. Both the methods are illustrated through numerical example.
Abstract: In this research, we propose a weighted class based
queuing (WCBQ) mechanism to provide class differentiation and to
reduce the load for the IMS (IP Multimedia Subsystem) presence
server (PS). The tasks of admission controller for the PS are
demonstrated. Analysis and simulation models are developed to
quantify the performance of WCBQ scheme. An optimized dropping
time frame has been developed based on which some of the preexisting
messages are dropped from the PS-buffer. Cost functions are
developed and simulation comparison has been performed with FCFS
(First Come First Served) scheme. The results show that the PS
benefits significantly from the proposed queuing and dropping
algorithm (WCBQ) during heavy traffic.
Abstract: In this paper, the processing of sonar signals has been
carried out using Minimal Resource Allocation Network (MRAN)
and a Probabilistic Neural Network (PNN) in differentiation of
commonly encountered features in indoor environments. The
stability-plasticity behaviors of both networks have been
investigated. The experimental result shows that MRAN possesses
lower network complexity but experiences higher plasticity than
PNN. An enhanced version called parallel MRAN (pMRAN) is
proposed to solve this problem and is proven to be stable in
prediction and also outperformed the original MRAN.
Abstract: Calcium is very important for communication among
the neurons. It is vital in a number of cell processes such as secretion,
cell movement, cell differentiation. To reduce the system of reactiondiffusion
equations of [Ca2+] into a single equation, two theories
have been proposed one is excess buffer approximation (EBA) other
is rapid buffer approximation (RBA). The RBA is more realistic than
the EBA as it considers both the mobile and stationary endogenous
buffers. It is valid near the mouth of the channel. In this work we have
studied the effects of different types of buffers on calcium diffusion
under RBA. The novel thing studied is the effect of sodium ions on
calcium diffusion. The model has been made realistic by considering
factors such as variable [Ca2+], [Na+] sources, sodium-calcium
exchange protein(NCX), Sarcolemmal Calcium ATPase pump. The
proposed mathematical leads to a system of partial differential equations
which has been solved numerically to study the relationships
between different parameters such as buffer concentration, buffer
disassociation rate, calcium permeability. We have used Forward
Time Centred Space (FTCS) approach to solve the system of partial
differential equations.
Abstract: In this paper, parallelism in the solution of Ordinary
Differential Equations (ODEs) to increase the computational speed is
studied. The focus is the development of parallel algorithm of the two
point Block Backward Differentiation Formulas (PBBDF) that can
take advantage of the parallel architecture in computer technology.
Parallelism is obtained by using Message Passing Interface (MPI).
Numerical results are given to validate the efficiency of the PBBDF
implementation as compared to the sequential implementation.
Abstract: It is sometimes difficult to differentiate between
innocent murmurs and pathological murmurs during auscultation. In
these difficult cases, an intelligent stethoscope with decision support
abilities would be of great value. In this study, using a dog model,
phonocardiographic recordings were obtained from 27 boxer dogs
with various degrees of aortic stenosis (AS) severity. As a reference
for severity assessment, continuous wave Doppler was used. The data
were analyzed with recurrence quantification analysis (RQA) with
the aim to find features able to distinguish innocent murmurs from
murmurs caused by AS. Four out of eight investigated RQA features
showed significant differences between innocent murmurs and
pathological murmurs. Using a plain linear discriminant analysis
classifier, the best pair of features (recurrence rate and entropy)
resulted in a sensitivity of 90% and a specificity of 88%. In
conclusion, RQA provide valid features which can be used for
differentiation between innocent murmurs and murmurs caused by
AS.
Abstract: The study describes chitosan membrane platform
modified with nanostructure pattern which using nanotechnology to
fabricate. The cell-substrate interaction between neuro-2a neuroblasts
cell lines and chitosan membrane (flat, nanostructure and
nanostructure pattern types) was investigated. The adhered
morphology of neuro-2a cells depends on the topography of chitosan
surface. We have found that neuro-2a showed different morphogenesis
when cells adhered on flat and nanostructure chitosan membrane. The
cell projected area of neuro-2a on flat chitosan membrane is larger
than on nanostructure chitosan membrane. In addition, neuro-2a cells
preferred to adhere on flat chitosan surface region than on
nanostructure chitosan membrane to immobilize and differentiation.
The experiment suggests surface topography can be used as a critical
mechanism to isolate group of neuro-2a to a particular rectangle area
on chitosan membrane. Our finding will provide a platform to take
patch clamp to record electrophysiological behavior about neurons in
vitro in the future.
Abstract: Mobile Ad hoc networks (MANETs) are collections
of wireless mobile nodes dynamically reconfiguring and collectively
forming a temporary network. These types of networks assume
existence of no fixed infrastructure and are often useful in battle-field
tactical operations or emergency search-and-rescue type of
operations where fixed infrastructure is neither feasible nor practical.
They also find use in ad hoc conferences, campus networks and
commercial recreational applications carrying multimedia traffic. All
of the above applications of MANETs require guaranteed levels of
performance as experienced by the end-user. This paper focuses on
key challenges in provisioning predetermined levels of such Quality
of Service (QoS). It also identifies functional areas where QoS
models are currently defined and used. Evolving functional areas
where performance and QoS provisioning may be applied are also
identified and some suggestions are provided for further research in
this area. Although each of the above functional areas have been
discussed separately in recent research studies, since these QoS
functional areas are highly correlated and interdependent, a
comprehensive and comparative analysis of these areas and their
interrelationships is desired. In this paper we have attempted to
provide such an overview.
Abstract: School physical education, through its objectives and
contents, efficiently valorizes the pupils- abilities, developing them,
especially the coordinative skill component, which is the basis of
movement learning, of the development of the daily motility and also
of the special, refined motility required by the practice of certain
sports. Medium school age offers the nervous and motor substratum
needed for the acquisition of complex motor habits, a substratum that
is essential for the coordinative skill. Individuals differ as to the level
at which this function is performed, the extent to which this function
turns an individual into a person that is adapted and adaptable to
complex and various situations. Spatio-temporal orientation, together
with movement combination and coupling, and with kinesthetic,
balance, motor reaction, movement transformation and rhythm
differentiation form the coordinative skills. From our viewpoint,
these are characteristic features with high levels of manifestation in a
complex psychomotor act - valorizing the quality of one-s talent - as
well as indices pertaining to one-s psychomotor intelligence and
creativity.
Abstract: Using DNA microarrays the comparative analysis of a
gene expression profiles is carried out in a liver and kidneys of pigs.
The hypothesis of a cross hybridization of one probe with different
cDNA sites of the same gene or different genes is checked up, and it
is shown, that cross hybridization can be a source of essential errors
at revealing of a key genes in organ-specific transcriptome. It is
reveald that distinctions in profiles of a gene expression are well coordinated
with function, morphology, biochemistry and histology of
these organs.