Abstract: In the past few years, high consumption of soft drinks
has attracted negative attention world-wide due to its possible
adverse effects, leading the health conscious people to find
alternative nutraceutical or herbal health drinks. In the present study,
a nutraceutical soft drink was developed utilizing some easily
available and well known traditional herbs having nutritional
potential. The key ingredients were selected as bael, amla, lemon
juice, ashwagandha and poppy seeds based on their household routine
use in the summer with proven refreshing, cooling and energetic
feeling since ages. After several trials made, the final composition of
nutraceutical summer soft drink was selected as most suitable
combination based on the taste, physicochemical, microbial and
organoleptic point of view. The physicochemical analysis of the
prepared drink found to contain optimum level of titratable acidity,
total soluble solids and pH which were in accordance of the
commercial recommendations. There were no bacterial colonies
found in the product therefore found within limits. During the nine
point’s hedonic scale sensory evaluation, the drink was strongly liked
for colour, taste, flavour and texture. The formulation was found to
contain flavonoids (80mg/100ml), phenolics (103mg/100ml), vitamin
C (250mg/100ml) and has antioxidant potential (75.52%) apart from
providing several other essential vitamins, minerals and healthy
components. The developed nutraceutical drink provides an
economical and feasible option for the consumers with very good
taste combined with potential health benefits. The present drink is
potentially capable to replace the synthetic soft drinks available in the
market.
Abstract: Chitosan polyacrylic acid composite membranes were
prepared by a bulk polymerization method in presence of N, N'-
methylene bisacrylamide (crosslinker) and ammonium persulphate as
initiator. Membranes prepared from this copolymer in presence and
absence of Ag nanoparticles were characterized by measuring
mechanical and physical properties, water up-take and antibacterial
properties. The results obtained indicated that the prepared
membranes have antibacterial properties which increase with adding
Ag nanoparticles.
Abstract: Nitrification is essential to biological processes
designed to remove ammonia and/or total nitrogen. It removes excess
nitrogenous compound in wastewater which could be very toxic to
the aquatic fauna or cause serious imbalance of such aquatic
ecosystem. Efficient nitrification is linked to an in-depth knowledge
of the structure and dynamics of the nitrifying community structure
within the wastewater treatment systems. In this study, molecular
technique was employed for characterizing the microbial structure of
activated sludge [ammonia oxidizing bacteria (AOB) and nitrite
oxidizing bacteria (NOB)] in a municipal wastewater treatment with
intention of linking it to the plant efficiency. PCR based phylogenetic
analysis was also carried out. The average operating and
environmental parameters as well as specific nitrification rate of plant
was investigated during the study. During the investigation the average temperature was 23±1.5oC.
Other operational parameters such as mixed liquor suspended solids
and chemical oxygen demand inversely correlated with ammonia
removal. The dissolved oxygen level in the plant was constantly
lower than the optimum (between 0.24 and 1.267 mg/l) during this
study. The plant was treating wastewater with influent ammonia
concentration of 31.69 and 24.47 mg/L. The influent flow rates
(ML/Day) was 96.81 during period. The dominant nitrifiers include:
Nitrosomonas spp. Nitrobacter spp. and Nitrospira spp. The AOB
had correlation with nitrification efficiency and temperature. This
study shows that the specific ammonia oxidizing rate and the specific
nitrate formation rates can serve as good indicator of the plant overall
nitrification performance.
Abstract: The aims of study were investigation on chemical
composition essential oil and the effect of extract of Coronilla varia
on antimicrobial and cytotoxicity activity. The essential oils of
Coronilla varia is obtained by hydrodistillation and analyzed by
(GC/MS) for determining their chemical composition and
identification of their components. Antibacterial activity of plant
extract was determined by disc diffusion method and anticancer
activity measured by MTT assay. The major components in essential
oil were Caryophyllene Oxide (60.19%), Alphacadinol (4.13%) and
Homoadantaneca Robexylic Acid (3.31%). The extracts from
Coronilla varia had interesting activity against Proteus mirabilis in
the concentration of 700 μg/disc and did not show any activity
against Staphylococus aureus, Bacillus subtillis, Klebsiella
pneumonia and Entrobacter cloacae. The positive control,
Ampicillin, Chloramphenicol and Cenphalothin had shown zone of
inhibition resistant all bacteria. The ethanol extract of Corohilla varia
inhibited on MCF7 cell lines. IC50 0.6(mg/ml) was the optimum
concentration of extract from Coronilla varia inhibition of cell line
growth. The MCF7 cancer cell line and Proteus mirabilis were more
sensitive to Coronilla varia ethanol extract.
Abstract: Groundwater is vital to the livelihoods and health of the majority of the people, since it provides almost the entire water resource for domestic, agricultural and industrial uses. Groundwater quality comprises the physical, chemical and bacteriological qualities. The present investigation was carried out to determine the physicochemical and bacteriological quality of the ground water sources in the residential areas of Karakulam Grama Panchayath in Thiruvananthapuram district, Kerala state in India. Karakulam is located in the eastern suburbs of Thiruvananthapuram city. The major drinking water source of the residents in the study area is wells. The present study aims to assess the portability and irrigational suitability of groundwater in the study area. The water samples were collected from randomly selected dug wells and bore wells in the study area during post monsoon and pre monsoon seasons of the year 2014 after a preliminary field survey. The physical, chemical and bacteriological parameters of the water samples were analyzed following standard procedures. The concentration of heavy metals (Cd, Pb and Mn) in the acid digested water samples were determined by using an Atomic Absorption Spectrophotometer. The results showed that the pH of well water samples ranged from acidic to alkaline level. In majority of well water samples (>54 %) the iron and magnesium content were found high in both the seasons studied, and the values were above the permissible limits of WHO drinking water quality standards. Bacteriological analyses showed that 63% of the wells were contaminated with total coliforms in both the seasons studied. Irrigational suitability of groundwater was assessed by determining the chemical indices like Sodium Percentage (%Na), Sodium Adsorption Ratio (SAR), Residual Sodium Carbonate (RSC), Permeability Index (PI), and the results indicate that the well water in the study area are good for irrigation purposes. Therefore, the study reveals the degradation of drinking water quality groundwater sources in Karakulam Grama Panchayath in Thiruvananthapuram District, Keralain terms of its chemical and bacteriological characteristics, and is not potable without proper treatment. In the study, more than 1/3rdof the well water samples tested were positive for total coliforms, and the bacterial contamination may pose threat to public health. The study recommends the need for periodic well water quality monitoring in the study area and to conduct awareness programs among the residents.
Abstract: Anammox is a novel and promising technology that has changed the traditional concept of biological nitrogen removal. The process facilitates direct oxidation of ammonical nitrogen under anaerobic conditions with nitrite as an electron acceptor without addition of external carbon sources. The present study investigated the feasibility of Anammox Hybrid Reactor (AHR) combining the dual advantages of suspended and attached growth media for biodegradation of ammonical nitrogen in wastewater. Experimental unit consisted of 4 nos. of 5L capacity AHR inoculated with mixed seed culture containing anoxic and activated sludge (1:1). The process was established by feeding the reactors with synthetic wastewater containing NH4-H and NO2-N in the ratio 1:1 at HRT (hydraulic retention time) of 1 day. The reactors were gradually acclimated to higher ammonium concentration till it attained pseudo steady state removal at a total nitrogen concentration of 1200 mg/l. During this period, the performance of the AHR was monitored at twelve different HRTs varying from 0.25-3.0 d with increasing NLR from 0.4 to 4.8 kg N/m3d. AHR demonstrated significantly higher nitrogen removal (95.1%) at optimal HRT of 1 day. Filter media in AHR contributed an additional 27.2% ammonium removal in addition to 72% reduction in the sludge washout rate. This may be attributed to the functional mechanism of filter media which acts as a mechanical sieve and reduces the sludge washout rate many folds. This enhances the biomass retention capacity of the reactor by 25%, which is the key parameter for successful operation of high rate bioreactors. The effluent nitrate concentration, which is one of the bottlenecks of anammox process was also minimised significantly (42.3-52.3 mg/L). Process kinetics was evaluated using first order and Grau-second order models. The first-order substrate removal rate constant was found as 13.0 d-1. Model validation revealed that Grau second order model was more precise and predicted effluent nitrogen concentration with least error (1.84±10%). A new mathematical model based on mass balance was developed to predict N2 gas in AHR. The mass balance model derived from total nitrogen dictated significantly higher correlation (R2=0.986) and predicted N2 gas with least error of precision (0.12±8.49%). SEM study of biomass indicated the presence of heterogeneous population of cocci and rod shaped bacteria of average diameter varying from 1.2-1.5 mm. Owing to enhanced NRE coupled with meagre production of effluent nitrate and its ability to retain high biomass, AHR proved to be the most competitive reactor configuration for dealing with nitrogen laden wastewater.
Abstract: Electrostatic interaction energy (ΔEEDL) is a part of the Extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory, which, together with van der Waals (ΔEVDW) and acid base (ΔEAB) interaction energies, has been extensively used to investigate the initial adhesion of bacteria to surfaces. Electrostatic or electrical double layer interaction energy is considerably affected by surface potential; however it cannot be determined experimentally and is usually replaced by zeta (ζ) potential via electrophoretic mobility. This paper focusses on the effect of ionic concentration as a function of pH and the effect of mineral grain size on ζ potential. It was found that both ionic strength and mineral grain size play a major role in determining the value of ζ potential for the adhesion of P. putida to hematite and quartz surfaces. Higher ζ potential values lead to higher electrostatic interaction energies and eventually to higher total XDLVO interaction energy resulting in bacterial repulsion.
Abstract: Batch production plants provide a wide range of
scheduling problems. In pharmaceutical industries a batch process
is usually described by a recipe, consisting of an ordering of tasks
to produce the desired product. In this research work we focused
on pharmaceutical production processes requiring the culture of
a microorganism population (i.e. bacteria, yeasts or antibiotics).
Several sources of uncertainty may influence the yield of the culture
processes, including (i) low performance and quality of the cultured
microorganism population or (ii) microbial contamination. For
these reasons, robustness is a valuable property for the considered
application context. In particular, a robust schedule will not collapse
immediately when a cell of microorganisms has to be thrown away
due to a microbial contamination. Indeed, a robust schedule should
change locally in small proportions and the overall performance
measure (i.e. makespan, lateness) should change a little if at all.
In this research work we formulated a constraint programming
optimization (COP) model for the robust planning of antibiotics
production. We developed a discrete-time model with a multi-criteria
objective, ordering the different criteria and performing a
lexicographic optimization. A feasible solution of the proposed
COP model is a schedule of a given set of tasks onto available
resources. The schedule has to satisfy tasks precedence constraints,
resource capacity constraints and time constraints. In particular
time constraints model tasks duedates and resource availability
time windows constraints. To improve the schedule robustness, we
modeled the concept of (a, b) super-solutions, where (a, b) are input
parameters of the COP model. An (a, b) super-solution is one in
which if a variables (i.e. the completion times of a culture tasks)
lose their values (i.e. cultures are contaminated), the solution can be
repaired by assigning these variables values with a new values (i.e.
the completion times of a backup culture tasks) and at most b other
variables (i.e. delaying the completion of at most b other tasks).
The efficiency and applicability of the proposed model is
demonstrated by solving instances taken from a real-life
pharmaceutical company. Computational results showed that
the determined super-solutions are near-optimal.
Abstract: Wireless Sensor Networks (WSNs) enable new
applications and need non-conventional paradigms for the protocol
because of energy and bandwidth constraints, In WSN, sensor node’s
life is a critical parameter. Research on life extension is based on
Low-Energy Adaptive Clustering Hierarchy (LEACH) scheme,
which rotates Cluster Head (CH) among sensor nodes to distribute
energy consumption over all network nodes. CH selection in WSN
affects network energy efficiency greatly. This study proposes an
improved CH selection for efficient data aggregation in sensor
networks. This new algorithm is based on Bacterial Foraging
Optimization (BFO) incorporated in LEACH.
Abstract: Chalcopyrite (CuFeS2) is the most common primary
mineral used for the commercial production of copper. The low
dissolution efficiency of chalcopyrite in sulfate media has prevented
an efficient industrial leaching of this mineral in sulfate media. Ferric
ions, bacteria, oxygen and other oxidants have been used as oxidizing
agents in the leaching of chalcopyrite in sulfate and chloride media
under atmospheric or pressure leaching conditions. Two leaching
methods were studied to evaluate chalcopyrite (CuFeS2) dissolution
in acid media. First, the conventional oxidative acid leaching method
was carried out using sulfuric acid (H2SO4) and potassium
dichromate (K2Cr2O7) as oxidant at atmospheric pressure. Second,
microwave-assisted acid leaching was performed using the
microwave accelerated reaction system (MARS) for same reaction
media. Parameters affecting the copper extraction such as leaching
time, leaching temperature, concentration of H2SO4 and
concentration of K2Cr2O7 were investigated. The results of
conventional acid leaching experiments were compared to the
microwave leaching method. It was found that the copper extraction
obtained under high temperature and high concentrations of oxidant
with microwave leaching is higher than those obtained
conventionally. 81% copper extraction was obtained by the
conventional oxidative acid leaching method in 180 min, with the
concentration of 0.3 mol/L K2Cr2O7 in 0.5M H2SO4 at 50 ºC, while
93.5% copper extraction was obtained in 60 min with microwave
leaching method under same conditions.
Abstract: The main objective of this study was to determine the
effects of Nitrogen fixing bacteria and manure application on the seed
yield and yield components in black cumin (Nigella sativa L.). The
experiment was carried out at the RAN Research Station in
Firouzkouh in 2012. A 4×4 factorial experiment, arranged in a
randomized complete blocks designed with three replications.
Nitrogen fixing bacteria at 4 levels (control, Azotobacter,
Azospirillum and Azotobacter + Azospirillum) and manure
application at 4 levels (0, 2.5, 5 and 7.5 ton ha-1) were used at this
investigation. The present results have shown that the highest height,
1000 seeds weight, seed number per follicle, follicle yield, seed yield
and harvest index were obtained after using Azotobacter and
Azospirillum, simultaneously. Manure application only effects on
follicle yield and by 5ton manure ha-1 the highest follicle yield
obtained. Results of this investigation showed that the maximum seed
yield obtained when Aotobacter+Azospirillum inoculated with black
cumin seeds and 5 ton manure ha-1 applied. According to the results
of this investigation the integrated management of Azotobacter and
Azospirillum with manure application is the best treatment for
achieving the maximum quantitative charactersitics of Black cumin.
Abstract: Food poisoning and infection by bacteria are of public
health significance to both developing and developed countries.
Samples of ogi (akamu) prepared from white and yellow variety of
maize sold in Uturu and Okigwe were analyzed together with the
laboratory prepared ogi for bacterial quality using the standard
microbiological methods. The analyses showed that both white and
yellow variety had total bacterial counts (cfu/g) of 4.0 ×107 and 3.9 x
107 for the laboratory prepared ogi while the commercial ogi had 5.2
x 107 and 4.9 x107, 4.9 x107 and 4.5 x107, 5.4 x107 and 5.0 x107 for
Eke-Okigwe, Up-gate and Nkwo-Achara market respectively. The
Staphylococcal counts ranged from 2.0 x 102 to 5.0 x102 and 1.0 x
102 to 4.0 x102 for the white and yellow variety from the different
markets while Staphylococcal growth was not recorded on the
laboratory prepared ogi. The laboratory prepared ogi had no Coliform
growth while the commercially prepared ogi had counts of 0.5 x103
to 1.6 x 103 for white variety and 0.3 x 103 to 1.1 x103 for yellow
variety respectively. The Lactic acid bacterial count of 3.5x106 and
3.0x106 was recorded for the laboratory ogi while the commercially
prepared ogi ranged from 3.2x106 to 4.2x106 (white variety) and 3.0
x106 to 3.9 x106 (yellow). The presence of bacteria isolates from the
commercial and laboratory fermented ogi showed that Lactobacillus
sp, Leuconostoc sp and Citrobacter sp were present in all the
samples, Micrococcus sp and Klebsiella sp were isolated from Eke-
Okigwe and ABSU-up-gate markets varieties respectively, E. coli
and Staphylococcus sp were present in Eke-Okigwe and Nkwo-
Achara markets while Salmonella sp were isolated from the three
markets. Hence, there are chances of contracting food borne diseases
from commercially prepared ogi. Therefore, there is the need for
sanitary measures in the production of fermented cereals so as to
minimize the rate of food borne pathogens during processing and
storage.
Abstract: Amoxicillin is an antibiotic which is widely used to
treat various infections in both human beings and animals. However,
when amoxicillin is released into the environment, it is a major
problem. Amoxicillin causes bacterial resistance to these drugs and
failure of treatment with antibiotics. Liquid membrane is of great
interest as a promising method for the separation and recovery of the
target ions from aqueous solutions due to the use of carriers for the
transport mechanism, resulting in highly selectivity and rapid
transportation of the desired metal ions. The simultaneous processes
of extraction and stripping in a single unit operation of liquid
membrane system are very interesting. Therefore, it is practical to
apply liquid membrane, particularly the HFSLM for industrial
applications as HFSLM is proved to be a separation process with
lower capital and operating costs, low energy and extractant with
long life time, high selectivity and high fluxes compared with solid
membranes. It is a simple design amenable to scaling up for industrial
applications. The extraction and recovery for (Amoxicillin) through
the hollow fiber supported liquid membrane (HFSLM) using
aliquat336 as a carrier were explored with the experimental data. The
important variables affecting on transport of amoxicillin viz.
extractant concentration and operating time were investigated. The
highest AMOX- extraction percentages of 85.35 and Amoxicillin
stripping of 80.04 were achieved with the best condition at 6 mmol/L
[aliquat336] and operating time 100 min. The extraction reaction
order (n) and the extraction reaction rate constant (kf) were found to
be 1.00 and 0.0344 min-1, respectively.
Abstract: This paper presents the performance of Integrated
Bacterial Foraging Optimization and Particle Swarm Optimization
(IBFO_PSO) technique in MANET routing. The BFO is a bio-inspired
algorithm, which simulates the foraging behavior of bacteria.
It is effectively applied in improving the routing performance in
MANET. In results, it is proved that the PSO integrated with BFO
reduces routing delay, energy consumption and communication
overhead.
Abstract: The article represents the results of isolation and
component chromatographic analysis of essential oils of Polygonum
L. plants growing in Kazakhstan in commercial reserves at the
territory of Kazakhstan. The results of research of antibacterial and
antifungal activity of isolated compounds have been represented.
Abstract: In the immunologic sense, clinical infection is a state
of failure of the immune system to combat the pathogenic weapon of
the bacteria invading the host. A motile gram negative vibroid
organism associated with marked mono and poly nuclear cell
responses was traced during the examination of a clinical material
from an infected common carp Cyprinus carpio. On primary plate
culture, growth was shown to be pure, dense population of an
Aeromonas-like colony morphotype. The pure isolate was found to
be; Aerobic, facultatively anaerobic, non-halophilic, grew at 0C, and
37C, oxidase positive utilizes glucose through fermentative pathway,
resist 0/129 and novobiocin, produces alanine and lysine
decarboxylases but non-producing ornithine dehydrolases. Tests for
the in vitro determinants of pathogenicity has shown to be; Betahaemolytic
onto blood agar, gelatinase, casienase and amylase
producer. Three in vivo determinants of pathogenicity were tested as,
the lethal dose fifty, the pathogenesis and pathogenicity. It was
evident that 0.1 milliliter of the causal bacterial cell suspension of a
density 1 x 107 CFU/ml injected intramuscularly into an average of
100gms fish toke five days incubation period, then at the day six
morbidity and mortality were initiated. LD50 was recorded at the day
12 post-infection. Use of an LD50 doses to study the pathogenicity,
reveals mononuclear and polynuclear cell responses, on examining
the stained direct films of the clinical materials from the
experimentally infected fish. Re-isolation tests confirm that the reisolant
is same. The course of the infection in natural case was shown
manifestation of; skin ulceration, haemorrhage and descaling. On
evisceration, the internal organs were shown; congestion in the
intestines, spleen and, air sacs. The induced infection showed a
milder form of these manifestations. The grading of the virulence of
this organism was virulent causing chronic course of infections as
indicated from the pathogenesis and pathogenicity studies. Thus the
infectious bacteria were consistent with Aeromonas hydrophila, and
the infection was chronic.
Abstract: Whey is the lactose rich by-product of the dairy
industry, having good amount of nutrient reservoir. Most abundant
nutrients are lactose, soluble proteins, lipids and mineral salts.
Disposing of whey by most of milk plants which do not have proper
pre-treatment system is the major issue. As a result of which, there
can be significant loss of potential food and energy source. Thus,
whey has been explored as the substrate for the synthesis of different
value added products such as enzymes. β-galactosidase is one of the
important enzymes and has become the major focus of research due
to its ability to catalyze both hydrolytic as well as
transgalactosylation reaction simultaneously. The enzyme is widely
used in dairy industry as it catalyzes the transformation of lactose to
glucose and galactose, making it suitable for the lactose intolerant
people. The enzyme is intracellular in both bacteria and yeast,
whereas for molds, it has an extracellular location. The present work
was carried to utilize the whey for the production of β-galactosidase
enzyme using both yeast and fungal cultures. The yeast isolate
Kluyveromyces marxianus WIG2 and various fungal strains have
been used in the present study. Different disruption techniques have
also been investigated for the extraction of the enzyme produced
intracellularly from yeast cells. Among the different methods tested
for the disruption of yeast cells, SDS-chloroform showed the
maximum β-galactosidase activity. In case of the tested fungal
cultures, Aureobasidium pullulans NCIM 1050 was observed to be
the maximum extracellular enzyme producer.
Abstract: Methicillin/multiple-resistant Staphylococcus aureus
(MRSA) are infectious bacteria that are resistant to common
antibiotics. A previous in silico study in our group has identified a
hypothetical protein SAV1226 as one of the potential drug targets. In
this study, we reported the bioinformatics characterization, as well as
cloning, expression, purification and kinetic assays of hypothetical
protein SAV1226 from methicillin/vancomycin-resistant
Staphylococcus aureus Mu50 strain. MALDI-TOF/MS analysis
revealed a low degree of structural similarity with known proteins.
Kinetic assays demonstrated that hypothetical protein SAV1226 is
neither a domain of an ATP dependent dihydroxyacetone kinase nor
of a phosphotransferase system (PTS) dihydroxyacetone kinase,
suggesting that the function of hypothetical protein SAV1226 might
be misannotated on public databases such as UniProt and
InterProScan 5.
Abstract: Microbes have been used to solve environmental
problems for many years. The role of microorganism to sequester,
precipitate or alter the oxidation state of various heavy metals has
been extensively studied. Treatment using microorganism interacts
with toxic metal are very diverse. The purpose of this research is to
remove the mercury using Pseudomonas putida (P. putida), pure
culture ATTC 49128 at optimum growth parameters such as
techniques of culture, acclimatization time and speed of incubator
shaker. Thus, in this study, the optimum growth parameters of P.
putida were obtained to achieve the maximum of mercury removal.
Based on the optimum parameters of P. putida for specific growth
rate, the removal of two different mercury concentration, 1 ppm and
4 ppm were studied. From mercury nitrate solution, a mercuryresistant
bacterial strain which is able to reduce from ionic mercury
to metallic mercury was used to reduce ionic mercury. The overall
levels of mercury removal in this study were between 80% and 89%.
The information obtained in this study is of fundamental for
understanding of the survival of P. putida ATTC 49128 in mercury
solution. Thus, microbial mercury removal is a potential
bioremediation for wastewater especially in petrochemical industries
in Malaysia.
Abstract: Medicinal plants are now gaining attractiveness in
treatment of bacterial infections and food preservation. The objective
of this study was to assess antibacterial activity of some medicinal
plants on pathogenic bacteria. Screening of antibacterial activity of
aqueous and methanol extracts of some plants: Jojoba, Ginger, Sage,
Thyme and Clove against Bacillus cereus, Salmonella typhimurium,
Staphylococcus aureus, Clostridium perfringens and Escherichia coli
were investigated. Antibacterial activity was performed by agar
diffusion and disc diffusion method. Jatropha, Jojoba, Clove and
Ginger extracts showed notable bacterial activity in the first
screening step then selected to be tested against Bacillus cereus
(Gram+), Staphylococcus aureus (Gram+) and Salmonella
typhimurium (Gram−) and their effect was compared using
antibiotics as control. Screening results showed potential antibacterial
activity of the tested plant extracts against the screened bacterial
strains. It was found that methanol extracts exhibited higher
antibacterial activity than aqueous extracts. Methanol extract of
Jatropha showed the highest inhibition zone against Staphylococcus
aureus (Gram+) with 24.00 mm diameter, compared to the other
plant extracts followed by clove. Meanwhile, the inhibition zones of
methanol extracts of Jojoba and Ginger were the same (12mm).The
Gram-positive bacteria were found to be more sensitive to aqueous
and methanol extracts than Gram-negative bacteria.