Abstract: This paper is aimed at proposing a rhombus shaped
wearable fractal antenna for wireless communication systems. The
geometrical descriptors of the antenna have been obtained using
bacterial foraging optimization (BFO) for wide band operation. The
method of moment based IE3D software has been used to simulate
the antenna and observed that miniaturization of 13.08% has been
achieved without degrading the resonating properties of the proposed
antenna. An analysis with different substrates has also been done in
order to evaluate the effectiveness of electrical permittivity on the
presented structure. The proposed antenna has low profile, light
weight and has successfully demonstrated wideband and multiband
characteristics for wearable electronic applications.
Abstract: The quality and condition of perishable products
delivered to the market and their subsequent selling prices are
directly affected by the care taken during harvesting and handling.
Mechanical injury, in fact, occurs at all stages, from pre-harvest
operations through post-harvest handling, packing and transport to
the market. The main implications of this damage are the reduction of
the product’s quality and economical losses related to the shelf life
diminution. For most perishable products, the shelf life is relatively
short and it is typically dictated by microbial growth related to the
application of dynamic and static loads during transportation. This
paper presents the correlation between vibration levels and
microbiological growth on strawberries and woodland strawberries
and detects the presence of volatile organic compounds (VOC) in
order to develop an intelligent logistic unit capable of monitoring
VOCs using a specific sensor system. Fresh fruits were exposed to
vibrations by means of a vibrating table in a temperature-controlled
environment. Microbiological analyses were conducted on samples,
taken at different positions along the column of the crates. The values
obtained were compared with control samples not exposed to
vibrations and the results show that different positions along the
column influence the development of bacteria, yeasts and filamentous
fungi.
Abstract: In this article, the antibiogram and heavy metal
resistance profile of the bacteria isolated from total 34 studied
animals (Pelophylax ridibundus = 12; Mauremys rivulata = 14;
Natrix natrix = 8) captured around the Biga Stream, are described.
There was no database information on antibiogram and heavy metal
resistance profile of bacteria from these area’s amphibians and
reptiles.
A total of 200 bacteria were successfully isolated from cloaca and
oral samples of the aquatic amphibians and reptiles as well as from
the water sample. According to Jaccard’s similarity index, the degree
of similarity in the bacterial flora was quite high among the
amphibian and reptile species under examination, whereas it was
different from the bacterial diversity in the water sample. The most
frequent isolates were A. hydrophila (31.5%), B. pseudomallei
(8.5%), and C. freundii (7%). The total numbers of bacteria obtained
were as follows: 45 in P. ridibundus, 45 in N. natrix 30 in M.
rivulata, and 80 in the water sample. The result showed that
cefmetazole was the most effective antibiotic to control the bacteria
isolated in this study and that approximately 93.33% of the bacterial
isolates were sensitive to this antibiotic. The multiple antibiotic
resistances (MAR) index indicated that P. ridibundus (0.95) > N.
natrix (0.89) > M. rivulata (0.39). Furthermore, all the tested heavy
metals (Pb+2, Cu+2, Cr+3, and Mn+2) inhibit the growth of the bacterial
isolates at different rates. Therefore, it indicated that the water source
of the animals was contaminated with both antibiotic residues and
heavy metals.
Abstract: The biodegradable family of polymers
polyhydroxyalkanoates is an interesting substitute for convectional
fossil-based plastics. However, the manufacturing and environmental
impacts associated with their production via intracellular bacterial
fermentation are strongly dependent on the raw material used and on
energy consumption during the extraction process, limiting their
potential for commercialization. Industrial wastewater is studied in
this paper as a promising alternative feedstock for waste valorization.
Based on results from laboratory and pilot-scale experiments, a
conceptual process design, techno-economic analysis and life cycle
assessment are developed for the large-scale production of the most
common type of polyhydroxyalkanoate, polyhydroxbutyrate.
Intracellular polyhydroxybutyrate is obtained via fermentation of
microbial community present in industrial wastewater and the
downstream processing is based on chemical digestion with
surfactant and hypochlorite. The economic potential and
environmental performance results help identifying bottlenecks and
best opportunities to scale-up the process prior to industrial
implementation. The outcome of this research indicates that the
fermentation of wastewater towards PHB presents advantages
compared to traditional PHAs production from sugars because the
null environmental burdens and financial costs of the raw material in
the bioplastic production process. Nevertheless, process optimization
is still required to compete with the petrochemicals counterparts.
Abstract: Freshly laid eggs from green turtles, Chelonia mydas,
were randomly collected from Ras Al-Hadd Reserve, Oman.
Eggshells taken from eggs and sand collected from the body chamber
were analyzed for eight heavy metals (Al, Br, Cd, Co, Cu, Fe, S, and
Zn) using inductively coupled plasma mass spectrometry (ICP).
Heavy metal concentrations varied significantly (P
Abstract: Paraffinic oils were submitted to microbial action. The
microorganisms consisted of bacteria of the genera Pseudomonas sp.
and Bacillus lincheniforms. The alterations in interfacial tension were
determined using a tensometer and applying the hanging drop
technique at room temperature (299 K ±275 K). The alteration in the
constitution of the paraffins was evaluated by means of gas
chromatography. The microbial activity was observed to reduce
interfacial tension by 54 to 78%, as well as consuming the paraffins
C19 to C29 and producing paraffins C36 to C44. The LIFirr technique
made it possible to determine the microbial action quickly.
Abstract: In this paper, we discuss the performance of applying
hybrid spiral dynamic bacterial chemotaxis (HSDBC) optimisation
algorithm on an intelligent controller for a differential drive robot. A
unicycle class of differential drive robot is utilised to serve as a basis
application to evaluate the performance of the HSDBC algorithm. A
hybrid fuzzy logic controller is developed and implemented for the
unicycle robot to follow a predefined trajectory. Trajectories of
various frictional profiles and levels were simulated to evaluate the
performance of the robot at different operating conditions. Controller
gains and scaling factors were optimised using HSDBC and the
performance is evaluated in comparison to previously adopted
optimisation algorithms. The HSDBC has proven its feasibility in
achieving a faster convergence toward the optimal gains and resulted
in a superior performance.
Abstract: Interaction of Schiff base complexes of Iron and
Manganese: Iron [N, N’ Bis (5- (triphenyl phosphonium methyl)
salicylidene) -1, 2 ethanediamine) chloride, [Fe Salen]Cl; Manganese
[N, N’ Bis (5- (triphenyl phosphonium methyl) salicylidene) -1, 2
ethanediamine) acetate, were investigated by spectroscopic and
isothermal titration calorimetry techniques (ITC).
The absorbance spectra of complexes have shown hyper and
hypochromism in the presence of DNA that is indication of
interaction of complexes with DNA. The linear dichroism (LD)
measurements confirmed the bending of DNA in the presence of
complexes.
Furthermore, Isothermal titration calorimetry experiments
approved that complexes bound to DNA on the base of both
electrostatic and hydrophobic interactions. More, ITC profile exhibits
the existence of two binding phases for the complexes. Antibacterial
activity of ligand and complexes were tested in vitro to evaluate their
activity against the gram positive and negative bacteria.
Abstract: The Algeria by its location offers a rich and diverse
vegetation. A large number of aromatic and medicinal plants grow
spontaneously. The interest in these plants has continued to grow in
recent years. Their particular properties due to the essential oil
fraction can be utilized to treat microbial infections. To this end, and
in the context of the valuation of the Algerian flora, we became
interested in the species of the family Lamiaceae which is one of the
most used as a global source of spices. The plant on which we have
based our choice is a species of sage "Salvia officinalis" from the
Isser localized region within the province of Boumerdes. This work
focuses on the study of the antimicrobial activity of essential oil
extracted from the leaves of Salvia officinalis. The extraction is
carried out by essential oil hydrodistillation and reveals a yield of
1.06℅. The study of the antimicrobial activity of the essential oil by
the method of at aromatogramme shown that Gram positive bacteria
are most susceptible (Staphylococcus aureus and Bacillus subtilis)
with a strong inhibition of growth. The yeast Candida albicans
fungus Aspergillus niger and have shown moderately sensitive.
Abstract: Hybrid bioreactor having both suspended-growth and
attached-growth bacteria is found a novel and excellent bioreactor
system for treating the municipal wastewater containing inhibitory
substrates too. In this reactor a fraction of substrate is used by
suspended biomass and the remaining by attached biomass resulting
in the competition between the two growths for the substrate. The
combination of suspended and attached growth provides the system
with enhanced biomass concentration and sludge age more than those
in ASP. Similar to attached growth system, the hybrid bioreactor
ensures considerable efficiency for treating toxic and refractory
substances in wastewater. For the process design of hybrid bioreactor
a suitable mathematical model is required. Although various
mathematical models were developed on hybrid bioreactor in due
course of time in earlier research works, none of them was found
having a specific simplified solution of the corresponding models and
without having any drawback. To overcome this drawback authors
already developed a simplified mathematical model for process
design of a hybrid bioreactor. The present paper briefly highlights on
the various aspects of process design of an aerobic hybrid bioreactor
for the treatment of municipal wastewater.
Abstract: Comparing other methods of waste water treatment,
constructed wetlands are one of the most fascinating practices
because being a natural process they are eco-friendly have low
construction and maintenance cost and have considerable capability
of wastewater treatment. The current research was focused mainly on
comparison of Ranunculus muricatus and Typha latifolia as wetland
plants for domestic wastewater treatment by designing and
constructing efficient pilot scale horizontal subsurface flow
mesocosms. Parameters like chemical oxygen demand, biological
oxygen demand, phosphates, sulphates, nitrites, nitrates, and
pathogenic indicator microbes were studied continuously with
successive treatments. Treatment efficiency of the system increases
with passage of time and with increase in temperature. Efficiency of
T. latifolia planted setups in open environment was fairly good for
parameters like COD and BOD5 which was showing reduction up to
82.5% for COD and 82.6% for BOD5 while DO was increased up to
125%. Efficiency of R. muricatus vegetated setup was also good but
lowers than that of T. latifolia planted showing 80.95% removal of
COD and BOD5. Ranunculus muricatus was found effective in
reducing bacterial count in wastewater. Both macrophytes were
found promising in wastewater treatment.
Abstract: Enterococci are important inhabitants of the animal
intestine and are widely used in probiotic products. A probiotic strain
is expected to possess several desirable properties in order to exert
beneficial effects. Therefore, the objective of this study was to
isolate, characterize and identify Enterococcus sp. from chicken cecal
and fecal samples to determine potential probiotic properties.
Enterococci were isolated from chicken ceca and feces of thirty three
clinically healthy chickens from a local farm. In vitro studies were
performed to assess antibacterial activity of the isolated LAB (using
agar well diffusion and cell free supernatant broth technique against
Salmonella enterica serotype Enteritidis), survival in acidic
conditions, resistance to bile salts, and their survival during simulated
gastric juice conditions at pH 2.5. Isolates were identified by
biochemical carbohydrate fermentation patterns using an API 50
CHL kit and API ZYM kits and by sequenced 16S rDNA. An isolate
belonging to E. faecium species exhibited inhibitory effect against S.
enteritidis. This isolate producing a clear zone as large as 10.30 mm
or greater and was able to grow in the coculture medium and at the
same time, inhibited the growth S. enteritidis. In addition, E. faecium
exhibited significant resistance under highly acidic conditions at pH
2.5 for 8 h and survived well in bile salt at 0.2% for 24 h and showing
ability to survive in the presence of simulated gastric juice at pH 2.5.
Based on these results, E. faecium isolate fulfills some of the criteria
to be considered as a probiotic strain and therefore, could be used as a
feed additive with good potential for controlling S. Enteritidis in
chickens. However, in vivo studies are needed to determine the safety
of the strain.
Abstract: Power systems are operating under stressed condition
due to continuous increase in demand of load. This can lead to
voltage instability problem when face additional load increase or
contingency. In order to avoid voltage instability suitable size of
reactive power compensation at optimal location in the system is
required which improves the load margin. This work aims at
obtaining optimal size as well as location of compensation in the 39-
bus New England system with the help of Bacteria Foraging and
Genetic algorithms. To reduce the computational time the work
identifies weak candidate buses in the system, and then picks only
two of them to take part in the optimization. The objective function is
based on a recently proposed voltage stability index which takes into
account the weighted average sensitivity index is a simpler and faster
approach than the conventional CPF algorithm. BFOA has been
found to give better results compared to GA.
Abstract: Lipases constitute one of the most important groups of
industrial enzymes that catalyze the hydrolysis of triacylglycerol to
glycerol and fatty acids. Muscarinic antagonist relieves smooth
muscle spasm of the gastrointestinal tract and effect on the
cardiovascular system. In this research the effect of a muscarinic
antagonist on the lipase activity of Pseudomonas aeruginosa was
studied. Lineweaver–Burk plot showed that the drug inhibited the
enzyme by competitive inhibition. The IC50 value (0.16 mM) and Ki
(0.03 mM) of the drug revealed the drug bound to enzyme with high
affinity. Determination of enzyme activity in various pH and
temperature showed that the maximum activity of lipase was at pH 8
and 60oC both in presence and absence of the drug.
Abstract: In this research paper were investigated the main
regularities of a radical bromination reaction of decalin. There had
been studied the temperature effect, durations of reaction, frequency
rate of process, a ratio of initial components, type and number of the
initiator on decalin bromination degree.
There were specified optimum conditions of synthesis of a
perbromodecalin by the method of a decalin bromination. There are
developed the technological flowchart of receiving a
perbromodecalin and the mass balance of process on the first and the
subsequent loadings of components.
The results of research of antibacterial and antifungal activity of
synthesized bromoderivatives have been represented.
Abstract: The influences of cell-free solutions (CFSs) of lactic
acid bacteria (LAB) on cadaverine and other biogenic amines
production by Listeria monocytogenes and Staphylococcus aureus
were investigated in lysine decarboxylase broth (LDB) using HPLC.
Cell free solutions were prepared from Lactococcus lactis subsp.
lactis, Leuconostoc mesenteroides subsp. cremoris, Pediococcus
acidilactici and Streptococcus thermophiles. Two different
concentrations that were 50% and 25% CFS and the control without
CFSs were prepared. Significant variations on biogenic amine
production were observed in the presence of L. monocytogenes and S.
aureus (P < 0.05). The function of CFS on biogenic amine production
by foodborne pathogens varied depending on strains and specific
amine. Cadaverine formation by L. monocytogenes and S. aureus in
control were 500.9 and 948.1 mg/L, respectively while the CFSs of
LAB induced 4-fold lower cadaverine production by L.
monocytogenes and 7-fold lower cadaverine production by S. aureus.
The CFSs resulted in strong decreases in cadaverine and putrescine
production by L. monocytogenes and S. aureus, although remarkable
increases were observed for histamine, spermidine, spermine,
serotonin, dopamine, tyramine and agmatine in the presence of LAB
in lysine decarboxylase broth.
Abstract: Doxorubicin, also known as Adriamycin, is an
anthracycline class of drug used in cancer chemotherapy. It is used in
the treatment of non-Hodgkin’s lymphoma, multiple myeloma, acute
leukemia, breast cancer, lung cancer, endometrium cancer and ovary
cancers. It functions via intercalating DNA and ultimately killing
cancer cells. The major side effects of doxorubicin are hair loss,
myelosuppression, nausea & vomiting, oesophagitis, diarrhea, heart
damage and liver dysfunction. The minor modifications in the
structure of compound exhibit large variation in the biological
activity, has prompted us to carry out the synthesis of sulfonamide
derivatives. Sulfonamide is an important feature with broad spectrum
of biological activity such as antiviral, antifungal, diuretics, antiinflammatory,
antibacterial and anticancer activities. Structure of the
synthesized compound N-(1-methyl-2-oxo-2-N-methyl anilinoethyl)
benzene sulfonamide confirmed by proton nuclear magnetic
resonance (1H NMR),13C NMR, Mass and FTIR spectroscopic tools
to assure the position of all protons and hence stereochemistry of the
molecule. Further we have reported the binding potential of
synthesized sulfonamide analogues in comparison to doxorubicin
drug using Auto Dock 4.2 software. Computational binding energy
(B.E.) and inhibitory constant (Ki) has been evaluated for the
synthesized compound in comparison of doxorubicin against Poly
(dA-dT).Poly (dA-dT) and Poly (dG-dC).Poly (dG-dC) sequences.
The in vitro cytotoxic study against human breast cancer cell lines
confirms the better anticancer activity of the synthesized compound
over currently in use anticancer drug doxorubicin. The IC50 value of
the synthesized compound is 7.12 μM whereas for doxorubicin is 7.2
μM.
Abstract: The aim of research was to define the relations
between volatile compounds, some parameters (pH, titratable acidity
(TA), total soluble solid (TSS), lactic acid bacteria count) and
consumer preference of commercial fermented milks. These relations
tend to be used for controlling and developing new fermented milk
product. Three leading commercial brands of fermented milks in
Thailand were evaluated by consumers (n=71) using hedonic scale
for four attributes (sweetness, sourness, flavour, and overall liking),
volatile compounds using headspace-solid phase microextraction
(HS-SPME) GC-MS, pH, TA, TSS and LAB count. Then the
relations were analyzed by principal component analysis (PCA). The
PCA data showed that all of four attributes liking scores were related
to each other. They were also related to TA, TSS and volatile
compounds. The related volatile compounds were mainly on
fermented produced compounds including acetic acid, furanmethanol,
furfural, octanoic acid and the volatiles known as artificial fruit
flavour (beta pinene, limonene, vanillin, and ethyl vanillin). These
compounds were provided the information about flavour addition in
commercial fermented milk in Thailand.
Abstract: For centuries humans have used the antimicrobial
properties of copper to their advantage. Yet, after all these years the
underlying mechanisms of copper mediated cell death in various
microbes remain unclear. We had explored the hypothesis that copper
mediated increased levels of lipid peroxidation in the membrane fatty
acids is responsible for increased killing in Escherichia coli. In this
study we show that in both gram positive (Staphylococcus aureus)
and gram negative (Pseudomonas aeruginosa) bacteria there is a
strong correlation between copper mediated cell death and increased
levels of lipid peroxidation. Interestingly, the non-spore forming
gram positive bacteria as well as gram negative bacteria show similar
patterns of cell death, increased levels of lipid peroxidation, as well
as genomic DNA degradation, however there is some difference in
loss in membrane integrity upon exposure to copper alloy surface.
Abstract: The management of Helicobacter pylori (H. pylori)
eradication is still a matter of discussion, full effectiveness is rarely
achieved, and it has many adverse effects. The use of probiotics may
be associated with better eradication rates and possibly prevention of
adverse events due to antibiotic therapy. The present clinical study
was undertaken to evaluate the efficacy of a specially designed
fermented milk product, containing Bifidobacterium lactis B420, on
the eradication of H. pylori infection in a prospective, randomized,
double-blind, controlled study in humans. Four test fermented milks
(FM) were specially designed in which counts of viable cells in all
products were 10^10 Log CFU. 100 mL-1 for Bifidobacterium lactis -
Bifidobacterium species 420. 190 subjects infected with H. pylori,
with previous diagnosis of functional dyspepsia according to Rome
III criteria entered the study. Bifidobacterium lactis B420,
administered twice a day for 90 days was not able to eradicate H.
pylori in Brazilian patients with functional dyspepsia.