Evaluation of Biofertilizer and Manure Effects on Quantitative Yield of Nigella sativa L.

The main objective of this study was to determine the effects of Nitrogen fixing bacteria and manure application on the seed yield and yield components in black cumin (Nigella sativa L.). The experiment was carried out at the RAN Research Station in Firouzkouh in 2012. A 4×4 factorial experiment, arranged in a randomized complete blocks designed with three replications. Nitrogen fixing bacteria at 4 levels (control, Azotobacter, Azospirillum and Azotobacter + Azospirillum) and manure application at 4 levels (0, 2.5, 5 and 7.5 ton ha-1) were used at this investigation. The present results have shown that the highest height, 1000 seeds weight, seed number per follicle, follicle yield, seed yield and harvest index were obtained after using Azotobacter and Azospirillum, simultaneously. Manure application only effects on follicle yield and by 5ton manure ha-1 the highest follicle yield obtained. Results of this investigation showed that the maximum seed yield obtained when Aotobacter+Azospirillum inoculated with black cumin seeds and 5 ton manure ha-1 applied. According to the results of this investigation the integrated management of Azotobacter and Azospirillum with manure application is the best treatment for achieving the maximum quantitative charactersitics of Black cumin.




References:
[1] Gyaneshwar, P., Naresh Kumar, G., Parekh, L.J., Poole, P.S., 2002. Role
of Soil Microorganisms in Improving P Nutrition of Plants. Plant. Soil.
245: 83-93.
[2] Haj Seyed Hadi, M.R., Darzi, M.T., Riazi, G.H., Ghandehari, Z., 2011.
Effects of Vermicompost and Amino Acids on the Flower Yield and
Essential Oil Production from Matricaria Chamomile L. J. Med. Plants
Res. 5(23):5611-5617.
[3] Patel, B.S., Patel, J.C., Sadaria, S.G., 1996. Response of Blond Psyllium
(Plantago ovata) to Irrigation and Phosphorus. Indian J. Agron. 41:
311314.
[4] Bendahou, M., Muselli, A., Grignon-Dubois, M., Benyoucef, M.,
Desjobert, J.M., Bernardini, A.F., Costa, J., 2008. Antimicrobial
Activity and Chemical Composition of Origanum glandulosum Desf.
Essential Oil and Extract Obtained by Microwave Extraction:
comparison with Hydrodistillation. Food Chem. 106: 132-139.
[5] Badary, O.A., Al-Shabanah, O.A., Nagi, M.N., Al-Rikabi, A.C.,
Elmazar, M.M., 1999. Inhibition of Benzo(a)Pyrene-Induced Forestomach Carcinogenesis in Mice by Thymoquinone. Eur. J. Cancer.
Prev. 8: 435-40.
[6] Ivankovic, S., Stojkovic, R., Jukic, M., Milos, M., Jurin, M., 200). The
Antitumor Activity of Thymoquinone and Thymohydroquinone in vitro
and in vivo. Exp. Oncol. 28 (3): 220-224.
[7] Sarig, S., Blum, A., Okon, Y., 1988. Improvement of the Water Status
Andyield of Field-Grown Grain Sorghum (Sorghum Bicolor) by
Inoculation with Azospirillum Brasilense. J. Agric. Sci. 110:271-277.
[8] Das, K., Dang, R., Shivananda, T.N., Sekeroglu, N., 2007. Influence
Obiofertilizers on the Biomass Yield and Nutrient Content in Stevia
Rebaudiana Bert. Grown in Indian Subtropics. J. Med. Plants Res.1
(1):5-8.
[9] Verma, N.K., 2011. Integrated Nutrient Management in Winter Maize
(Zea mays L.) Sown at Different Dates. Afr. J. Plant Breed. Crop Sci.
3(8):161-167.
[10] Pereyra, M.A., Garcia, P., Colabelli, M.N., Barassi, C.A., Creus, C.M.,
2012. A Better Water Status in Wheat Seedlings Induced by
Azospirillum under Osmotic Stress is Related to Morphological Changes
in Xylem Vessels of the Coleoptile. Appl. Soil Ecol. 53:94-97.
[11] Kolb, W., Martin, P., 1985. Response of Plant Roots to Inoculation with
Azospirillum Brasilense and to Application of Indole Acetic Acid. In
Azospirillum. III. Genetics, Physiology, Ecology. Edited by W.
Klingmüller. Springer-Verlag, Berlin, Heidelberg, pp. 215-221.
[12] Najm, A.A., Haj Seyed Hadi, M.R., Darzi, M.T., Rahi, A.R., Fazeli, F.,
2012. Effects of Integrated Management of Nitrogen Fertilizer and
Cattle Manure on the Leaf Cholorophyll, Yield and Tuber
Glycoalkaloids of Agria Potato. Commun. Soil. Sci. Plant Anal. 43: 912-
923.
[13] Millet, E., Feldman, M., 1986. Yield Response of a Common Spring
Wheat Cultivar to Inoculation with Azospirillum Brasilense at Various
Levels Ofnitrogen Fertilization. Plant Soil, 80:255-259.
[14] Warembourg, F.R., Dreessen, R., Vlassak, K., Lafont, F., 1987. Peculiar
Effect of Azospirillum Inoculation on Growth and Nitrogen Balance of
Winter Wheat (Triticum aestivum). Biol. Fertil. Soils 4:55-59.
[15] Nadjafi, F., Rezvani, P., 2002. Effects of Irrigation Regimes and Plant
Densities on Yield and Agronomic Characteristics of Isabgol (Plantago
ovata). Agric. Sci. Technol. 2:59-65.
[16] Bhaskara, R.K.V., Charyulu, P.B.B.N., 2005. Evaluation of effect of
Inoculation of Azospirillum on the Yield of Setaria Italica (L.). Afr.
JBiotechnol. 4(9):989-995.
[17] Haj Seyed Hadi, M.R., Darzi, M.T., Ghandehari, Z., 2012. Effects of
Irrigation Treatment and Azospirillum Inoculation on Yield and Yield
Component of Black Cumin (Nigella sativa L.). J. Med. Plants Res.
6(30): 4553-4561.
[18] Fulchieri M, Frioni L (1994). Azospirillum Inoculation on Maize (Zea
mays): Effect on Yield in a Field Experiment in Central Argentina. Soil.
Biol. Chem. 26:921-923.
[19] Caballero-Mellado, J.M., Carcano, M.M.G., Mascarua, E.M.A., 1992.
Field Inoculation of Wheat (Triticum aestivum) with Azospirillum
Brasilense under Temperate Climate. Symbiosis. 13:243-253.
[20] Barton, I.L., Johnson, G.V., Orbock, M.S., 1986. The Effect of
Azospirillum Brasilense on Iron Absorption and Translocation by
Sorghum. J. Plant nut. 9: 557-565.
[21] Murty, M.G., Ladha, J.K., 1987. Differential Colonization of
Azospirillum Lipoferum on Roots of Two Varieties of Rice (oryza
satival.). Biol. Fertil. Soils. 4: 3-7.