Abstract: Characterization and evaluation of the activity of Vespa basalis DPP-IV, which expressed in Spodoptera frugiperda 21 cells. The expression of rDPP-IV was confirmed by SDS–PAGE, Western blot analyses, LC-MS/MS and measurement of its peptidase specificity. One-step purification by Ni-NTA affinity chromatography and the total amount of rDPP-IV recovered was approximately 6.4mg per liter from infected culture medium; an equivalent amount would be produced by 1x109 infected Sf21 insect cells. Through the affinity purification led to highly stable rDPP-IV enzyme was recovered and with significant peptidase activity. The rDPP-IV exhibited classical Michaelis–Menten kinetics, with kcat/Km in the range of 10-500 mM-1×S-1 for the five synthetic substrates and optimum substrate is Ala-Pro-pNA. As expected in inhibition assay, the enzymatic activity of rDPP-IV was significantly reduced by 80 or 60% in the presence of sitagliptin (a DPP-IV inhibitor) or PMSF (a serine protease inhibitor), but was not apparently affected by iodoacetamide (a cysteine protease inhibitor).
Abstract: This study aims to demonstrate the quantification of
peptides based on isotope dilution surface enhanced Raman
scattering (IDSERS). SERS spectra of phenylalanine (Phe), leucine
(Leu) and two peptide sequences TGQIFK (T13) and
YSFLQNPQTSLCFSESIPTPSNR (T6) as part of the 22-kDa
human growth hormone (hGH) were obtained on Ag-nanoparticle
covered substrates. On the basis of the dominant Phe and Leu
vibrational modes, precise partial least squares (PLS) prediction
models were built enabling the determination of unknown T13 and
T6 concentrations. Detection of hGH in its physiological
concentration in order to investigate the possibility of protein
quantification has been achieved.
Abstract: A. niger XP isolated from Vietnam produces very low amount of acidic phytase with optimal pH at 2.5 and 5.5. The phytase production of this strain was successfully improved through gene cloning and expression. A 1.4 - kb DNA fragment containing the coding region of the phyA gene was amplified by PCR and inserted into the expression vector pPICZαA with a signal peptide α- factor, under the control of AOX1 promoter. The recombined plasmid was transformed into the host strain P. pastoris KM71H and X33 by electroporation. Both host strains could efficiently express and secret phytase. The multicopy strains were screened for over expression of phytase. All the selected multicopy strains of P. pastoris X33 were examined for phytase activity, the maximum phytase yield of 1329 IU/ml was obtained after 4 days of incubation in medium BMM. The recombinant protein with MW of 97.4 KW showed to be the only one protein secreted in the culture broth. Multicopy transformant P. pastoris X33 supposed to be potential candidate for producing the commercial preparation of phytase.
Abstract: Taking into account the importance of Lake Sevan and
Lake Sevan basin-s rivers for Armenian economy, the main goals of
our investigations were the documentation of water quality and the
biodiversity of invertebrates developed in Lake Sevan basin-s rivers
and selected tributaries.
Moderately satisfied ecological condition for the biodiversity of
Lake Sevan basin-s rivers has been established, and the changes in
species- composition of zoobenthos in Lake Sevan were detected.
A growing tendency of antibiotic resistance among E. coli isolates
in water resources has been shown.
Abstract: An immunomodulator bioproduct is prepared in a
batch bioprocess with a modified bacterium Pseudomonas
aeruginosa. The bioprocess is performed in 100 L Bioengineering
bioreactor with 42 L cultivation medium made of peptone, meat
extract and sodium chloride. The optimal bioprocess parameters were
determined: temperature – 37 0C, agitation speed - 300 rpm, aeration
rate – 40 L/min, pressure – 0.5 bar, Dow Corning Antifoam M-max.
4 % of the medium volume, duration - 6 hours. This kind of
bioprocesses are appreciated as difficult to control because their
dynamic behavior is highly nonlinear and time varying. The aim of
the paper is to present (by comparison) different models based on
experimental data.
The analysis criteria were modeling error and convergence rate.
The estimated values and the modeling analysis were done by using
the Table Curve 2D.
The preliminary conclusions indicate Andrews-s model with a
maximum specific growth rate of the bacterium in the range of
0.8 h-1.
Abstract: Ligula intestinalis is a three-host life-cycle
Pseudophyllidean Cestode which in its plerocercoid stage infests a
range of fresh water species. The objective of the present study was
the worm occurrence within planctonic copepods, fishes and
piscivorous birds and examine of parasite-hosts samples in the Lake
of Sattarkhan Dam (near the city of Ahar, East Azerbaijan, Iran).
Fish sample were collected with fyke and gill nets and the abdominal
cavity was examined for the presence of ligula. Zooplanktons were
captured using a planktonic net and occurrence of parasitic larval
form in the body cavity was determined. Piscivorous birds were
selected by telescope, they hunted and dissected for presence of
parasite eggs in their gut. Results indicated that prevalence of
infection was 16% for cyclopid copepoda and majority of infected
cyclopid were female Cyclops. Investigation of 310 fishes specimens
were indicated to infection of five species of cyprinid fishes. In
addition, results indicated to manipulation of six species of migratory
aquatic and semi aquatic birds by ligula. Obtained results are in
agreement by previous studies. Its definite in this study that all of
fishes in Sattarkhan Lake capable to infection, its important for
health because they capture by native people and it is documented
that ligula can be introduce as a zoonose. It's seemed that to prevent
from disperses of parasite and restricted of infection, biological
elimination can be effective and it's necessary to inform native
people about sanitation.
Abstract: The present work represents an investigation of the
hydrolysis of hull-less pumpkin (Cucurbita Pepo L.) oil cake protein
isolate (PuOC PI) by pepsin. To examine the effectiveness and
suitability of pepsin towards PuOC PI the kinetic parameters for
pepsin on PuOC PI were determined and then, the hydrolysis process
was studied using Response Surface Methodology (RSM). The
hydrolysis was carried out at temperature of 30°C and pH 3.00. Time
and initial enzyme/substrate ratio (E/S) at three levels were selected
as the independent parameters. The degree of hydrolysis, DH, was
mesuared after 20, 30 and 40 minutes, at initial E/S of 0.7, 1 and 1.3
mA/mg proteins. Since the proposed second-order polynomial model
showed good fit with the experimental data (R2 = 0.9822), the
obtained mathematical model could be used for monitoring the
hydrolysis of PuOC PI by pepsin, under studied experimental
conditions, varying the time and initial E/S. To achieve the highest
value of DH (39.13 %), the obtained optimum conditions for time
and initial E/S were 30 min and 1.024 mA/mg proteins.
Abstract: Lectins have a good scope in current clinical
microbiology research. In the present study evaluated the
antimicrobial activities of a D-galactose binding lectin (PnL) was
purified from the annelid, Perinereis nuntia (polychaeta) by affinity
chromatography. The molecular mass of the lectin was determined to
be 32 kDa as a single polypeptide by SDS-PAGE under both reducing
and non-reducing conditions. The hemagglutinating activity of the
PnL showed against trypsinized and glutaraldehyde-fixed human
erythrocytes was specifically inhibited by D-Gal, GalNAc,
Galβ1-4Glc and Galα1-6Glc. PnL was evaluated for in vitro
antibacterial screening studies against 11 gram-positive and
gram-negative microorganisms. From the screening results, it was
revealed that PnL exhibited significant antibacterial activity against
gram-positive bacteria. Bacillus megaterium showed the highest
growth inhibition by the lectin (250 μg/disc). However, PnL did not
inhibit the growth of gram-negative bacteria such as Vibrio cholerae
and Pseudomonas sp. PnL was also examined for in vitro antifungal
activity against six fungal phytopathogens. PnL (100 μg/mL) inhibited
the mycelial growth of Alternaria alternata (24.4%). These results
indicate that future findings of lectin applications obtained from
annelids may be of importance to life sciences.
Abstract: In this paper, an extended study is performed on the
effect of different factors on the quality of vector data based on a
previous study. In the noise factor, one kind of noise that appears in
document images namely Gaussian noise is studied while the previous
study involved only salt-and-pepper noise. High and low levels of
noise are studied. For the noise cleaning methods, algorithms that were
not covered in the previous study are used namely Median filters and
its variants. For the vectorization factor, one of the best available
commercial raster to vector software namely VPstudio is used to
convert raster images into vector format. The performance of line
detection will be judged based on objective performance evaluation
method. The output of the performance evaluation is then analyzed
statistically to highlight the factors that affect vector quality.
Abstract: Certain tRNA synthetases have developed highly accurate molecular machinery to discriminate their cognate amino acids. Those aaRSs achieve their goal via editing reaction in the Connective Polypeptide 1 (CP1). Recently mutagenesis studies have revealed the critical importance of residues in the CP1 domain for editing activity and X-ray structures have shown binding mode of noncognate amino acids in the editing domain. To pursue molecular mechanism for amino acid discrimination, molecular modeling studies were performed. Our results suggest that aaRS bind the noncognate amino acid more tightly than the cognate one. Finally, by comparing binding conformations of the amino acids in three systems, the amino acid binding mode was elucidated and a discrimination mechanism proposed. The results strongly reveal that the conserved threonines are responsible for amino acid discrimination. This is achieved through side chain interactions between T252 and T247/T248 as well as between those threonines and the incoming amino acids.
Abstract: Subjective loneliness describes people who feel a
disagreeable or unacceptable lack of meaningful social relationships,
both at the quantitative and qualitative level. The studies to be
presented tested an Italian 18-items self-report loneliness measure,
that included items adapted from scales previously developed,
namely a short version of the UCLA (Russell, Peplau and Cutrona,
1980), and the 11-items Loneliness scale by De Jong-Gierveld &
Kamphuis (JGLS; 1985). The studies aimed at testing the developed
scale and at verifying whether loneliness is better conceptualized as a
unidimensional (so-called 'general loneliness') or a bidimensional
construct, namely comprising the distinct facets of social and
emotional loneliness. The loneliness questionnaire included 2 singleitem
criterion measures of sad mood, and social contact, and asked
participants to supply information on a number of socio-demographic
variables. Factorial analyses of responses obtained in two
preliminary studies, with 59 and 143 Italian participants respectively,
showed good factor loadings and subscale reliability and confirmed
that perceived loneliness has clearly two components, a social and an
emotional one, the latter measured by two subscales, a 7-item
'general' loneliness subscale derived from UCLA, and a 6–item
'emotional' scale included in the JGLS. Results further showed that
type and amount of loneliness are related, negatively, to frequency of
social contacts, and, positively, to sad mood. In a third study data
were obtained from a nation-wide sample of 9.097 Italian subjects,
12 to about 70 year-olds, who filled the test on-line, on the Italian
web site of a large-audience magazine, Focus. The results again
confirmed the reliability of the component subscales, namely social,
emotional, and 'general' loneliness, and showed that they were
highly correlated with each other, especially the latter two.
Loneliness scores were significantly predicted by sex, age, education
level, sad mood and social contact, and, less so, by other variables –
e.g., geographical area and profession. The scale validity was
confirmed by the results of a fourth study, with elderly men and
women (N 105) living at home or in residential care units. The three
subscales were significantly related, among others, to depression, and
to various measures of the extension of, and satisfaction with, social
contacts with relatives and friends. Finally, a fifth study with 315
career-starters showed that social and emotional loneliness correlate
with life satisfaction, and with measures of emotional intelligence.
Altogether the results showed a good validity and reliability in the
tested samples of the entire scale, and of its components.
Abstract: Bacillus subtilis strain LB5 produced lipopeptide
antibiotic iturin A-2 in liquid medium. Crude extract
from cell-free supernatant of B. subtilis cultivated broth extracted
with n-butanol showed antifungal activity to conidial germination of
Colletotrichum gloeosporioides. The germination of conidia was
completely inhibited by crude extract. The ultrastructure of conidia
after treated with crude extract was found an accumulation of vesiclelike
material between cell wall and plasma membrane while this
accumulation was not observed in untreated and germinated conidia.
Besides, the cell wall was not affected by crude extract.
Abstract: In this paper, a robust statistics based filter to remove salt and pepper noise in digital images is presented. The function of the algorithm is to detect the corrupted pixels first since the impulse noise only affect certain pixels in the image and the remaining pixels are uncorrupted. The corrupted pixels are replaced by an estimated value using the proposed robust statistics based filter. The proposed method perform well in removing low to medium density impulse noise with detail preservation upto a noise density of 70% compared to standard median filter, weighted median filter, recursive weighted median filter, progressive switching median filter, signal dependent rank ordered mean filter, adaptive median filter and recently proposed decision based algorithm. The visual and quantitative results show the proposed algorithm outperforms in restoring the original image with superior preservation of edges and better suppression of impulse noise
Abstract: Pepper spray use has gained momentum since 1992
and although the active ingredient is readily available, it is considered
a weapon with restricted use in many regions, including The
Bahamas. In light of controversy in the literature regarding the
severity of presenting respiration complaints among individuals postacute
exposure of pepper spray inhalation, this descriptive case series
study was conducted to assess the respiratory status of persons
evaluated during a mass casualty in The Bahamas. Parameters noted
were patients- demographics and respiration severity determined via
clinical examination findings, disposition and follow-up review of the
20 persons. Their most common complaint was difficulty breathing
post exposure. Two required admission and stayed for
Abstract: The present study has been taken to explore the
screening of in vitro antimicrobial activities of D-galactose-binding
sponge lectin (HOL-30). HOL-30 was purified from the marine
demosponge Halichondria okadai by affinity chromatography. The
molecular mass of the lectin was determined to be 30 kDa with a
single polypeptide by SDS-PAGE under non-reducing and reducing
conditions. HOL-30 agglutinated trypsinized and glutaraldehydefixed
rabbit and human erythrocytes with preference for type O
erythrocytes. The lectin was subjected to evaluation for inhibition of
microbial growth by the disc diffusion method against eleven human
pathogenic gram-positive and gram-negative bacteria. The lectin
exhibited strong antibacterial activity against gram-positive bacteria,
such as Bacillus megaterium and Bacillus subtilis. However, it did
not affect against gram-negative bacteria such as Salmonella typhi
and Escherichia coli. The largest zone of inhibition was recorded of
Bacillus megaterium (12 in diameter) and Bacillus subtilis (10 mm in
diameter) at a concentration of the lectin (250 μg/disc). On the other
hand, the antifungal activity of the lectin was investigated against six
phytopathogenic fungi based on food poisoning technique. The lectin
has shown maximum inhibition (22.83%) of mycelial growth of
Botrydiplodia theobromae at a concentration of 100 μg/mL media.
These findings indicate that the lectin may be of importance to
clinical microbiology and have therapeutic applications.
Abstract: Positron emission particle tracking (PEPT) is a
technique in which a single radioactive tracer particle can be
accurately tracked as it moves. A limitation of PET is that in order to
reconstruct a tomographic image it is necessary to acquire a large
volume of data (millions of events), so it is difficult to study rapidly
changing systems. By considering this fact, PEPT is a very fast
process compared with PET.
In PEPT detecting both photons defines a line and the annihilation
is assumed to have occurred somewhere along this line. The location
of the tracer can be determined to within a few mm from coincident
detection of a small number of pairs of back-to-back gamma rays and
using triangulation. This can be achieved many times per second and
the track of a moving particle can be reliably followed. This
technique was invented at the University of Birmingham [1].
The attempt in PEPT is not to form an image of the tracer particle
but simply to determine its location with time. If this tracer is
followed for a long enough period within a closed, circulating system
it explores all possible types of motion.
The application of PEPT to industrial process systems carried out
at the University of Birmingham is categorized in two subjects: the
behaviour of granular materials and viscous fluids. Granular
materials are processed in industry for example in the manufacture of
pharmaceuticals, ceramics, food, polymers and PEPT has been used
in a number of ways to study the behaviour of these systems [2].
PEPT allows the possibility of tracking a single particle within the
bed [3]. Also PEPT has been used for studying systems such as: fluid
flow, viscous fluids in mixers [4], using a neutrally-buoyant tracer
particle [5].
Abstract: The Zung self-depression scale and Beck Anxiety Inventory were used to study the depression and anxiety levels of Armenian Crohn's disease patients, as well as to reveal the relation between emotional status and placebo effect of these patients. Despite of registered high levels of depression and anxiety, the high placebo rate during investigations was described. The importance of use of psychotherapies for optimal outcomes during treatments of Crohn's disease is obvious.
Abstract: In this paper, we propose a novel spatiotemporal fuzzy
based algorithm for noise filtering of image sequences. Our proposed algorithm uses adaptive weights based on a triangular membership
functions. In this algorithm median filter is used to suppress noise.
Experimental results show when the images are corrupted by highdensity
Salt and Pepper noise, our fuzzy based algorithm for noise filtering of image sequences, are much more effective in suppressing
noise and preserving edges than the previously reported algorithms such as [1-7]. Indeed, assigned weights to noisy pixels are very
adaptive so that they well make use of correlation of pixels. On the other hand, the motion estimation methods are erroneous and in highdensity noise they may degrade the filter performance. Therefore, our
proposed fuzzy algorithm doesn-t need any estimation of motion trajectory. The proposed algorithm admissibly removes noise without having any knowledge of Salt and Pepper noise density.
Abstract: Changing technology and increased constituent
demand for government services derive the need for governmental
responsiveness. The government organisations in the developing
countries will be under increased pressure to change their
bureaucratic systems to be able to respond rapidly to changing and
increasing requirements and rapid technology advancements. This
paper aims to present a conceptual framework for explaining the
main barriers and drivers of public e-service development. Therefore,
the framework provides a basic context within which the process and
practice of E-Service can be implemented successfully in the public
sector organisations. The framework is flexible enough to be adopted
by governments at different levels; national or local by developing
countries around the world.
Abstract: Majority of pepper farmers in Malaysia are using the
open-sun method for drying the pepper berries. This method is time
consuming and exposed the berries to rain and contamination. A
maintenance-friendly and properly enclosed dryer is therefore
desired. A dryer design with a solar collector and a chimney was
studied and adapted to suit the needs of small-scale pepper farmers in
Malaysia. The dryer will provide an environment with an optimum
operating temperature meant for drying pepper berries. The dryer
model was evaluated by using commercially available computational
fluid dynamic (CFD) software in order to understand the heat and
mass transfer inside the dryer. Natural convection was the only mode
of heat transportation considered in this study as in accordance to the
idea of having a simple and maintenance-friendly design. To
accommodate the effect of low buoyancy found in natural convection
driers, a biomass burner was integrated into the solar dryer design.