Abstract: This study aims to demonstrate the quantification of
peptides based on isotope dilution surface enhanced Raman
scattering (IDSERS). SERS spectra of phenylalanine (Phe), leucine
(Leu) and two peptide sequences TGQIFK (T13) and
YSFLQNPQTSLCFSESIPTPSNR (T6) as part of the 22-kDa
human growth hormone (hGH) were obtained on Ag-nanoparticle
covered substrates. On the basis of the dominant Phe and Leu
vibrational modes, precise partial least squares (PLS) prediction
models were built enabling the determination of unknown T13 and
T6 concentrations. Detection of hGH in its physiological
concentration in order to investigate the possibility of protein
quantification has been achieved.