Effect of Crude Extract from Bacillus Subtilis LB5 Cultivated Broth on Conidial Germination of Colletotrichum Gloeosporioides
Bacillus subtilis strain LB5 produced lipopeptide
antibiotic iturin A-2 in liquid medium. Crude extract
from cell-free supernatant of B. subtilis cultivated broth extracted
with n-butanol showed antifungal activity to conidial germination of
Colletotrichum gloeosporioides. The germination of conidia was
completely inhibited by crude extract. The ultrastructure of conidia
after treated with crude extract was found an accumulation of vesiclelike
material between cell wall and plasma membrane while this
accumulation was not observed in untreated and germinated conidia.
Besides, the cell wall was not affected by crude extract.
[1] A. L. Senghor, W. J. Liang and W. C. Ho. "Integrated control of
Colletotrichum gloeosporioides on mango fruit in Taiwan by the
combination of Bacillus subtilis and fruit bagging," Biolcontrol Sci.
Techn., vol. 17, pp. 865-870, Sep. 2007.
[2] B. C. Sutton. "The genus Glomerella and its anamorph Colletotrichum"
in Colletotrichum: Biology, Pathology and Control, J. A. Bailey and M.
J. Jeger, Eds, Wallingford: CAB International, 1992, pp. 1-26.
[3] C.C. Lin, "Disease survey and integrated control of diseases on forcing
culture on wax-apple fruit trees (Translation Journals style)," Research
Bulletin of Kaosiung District Agricultural Improvement Station, vol. 13,
pp 20-29, Dec. 2001.
[4] D. Romero, A. de Vicente, J. L. Olmos, J. C. D├ívila, and A. Pérez-
García. "Effect of lipopeptides of antagonistic strains of Bacillus subtilis
on the morphology and ultrastructure of the cucurbit fungal pathogen
Podosphaera fusca," J. of Appl Microbiol, vol 103, pp. 969-976, Oct.
2007.
[5] E. A. B. Emmert and J. Handelsman. 1999. "Biocontrol of plant disease:
a (Gram-) positive perspective," FEMS Microbiol. Lett., vol. 171, pp. 1-
9, Feb. 1999.
[6] I. L. Shih, C. Y. Lin, J. Y. Wu, and C. Hsieh. "Production of antifungal
lipopeptide from Bacillus subtilis in submerged fermentation using
shake and fermenter," Korea J. Chem. Eng., vol. 26, pp. 1652-1661,
Nov. 2009.
[7] J.M. Waller, "Colletotrichum diseases of perennial and other cash crop
(Book style with paper title and editor)," in Colletotrichum: biology,
pathology and control, J.A. Bailey and M.J. Jeger, Eds, UK: Redwood
Press, 1992, pp. 167-185.
[8] L. Thimon, F.Peypoux , J. Wallach, and G. Michel. "Effect of the
lipopeptide antibiotic, iturin A, on morphology and membrane
ultrastructure of yeast cells," FEMS Microbiol. Lett., vol. 128, pp. 101-
106, May 1995.
[9] O. Ruangwong, C. I. Chang, A. L. Senghor and W. J. Liang.
"Identification of antifungal compound produced by Bacillus
subtilis LB5 with ability to control anthracnose disease caused
by Colletotrichum gloeosporioides," Afr. J. Microbiol. Res., vol. 6, pp
3732-3738, Apr. 2012.
[10] P. F. Cannon, P. D. Bridge, and E. Monte. "Linking the past, present and
future of Colletotrichum systematics," in Colletotrichum. Host
Specificity, Pathology and Host-Pathogen Interaction, D. Prusky, S.
Freeman, and M.B. Dickman, Eds, St Paul, Minnesota: APS Press,
2000, pp. 1-20.
[11] P. Leroux. "Modes of action of agrochemicals against plant pathogenic
organisms," C. R. Biol., vol. 326, pp. 9-2, Jan 2003.
[12] R. Maget-Dana, and F. Peypoux. "Iturins, a speial class of pore-forming
lipopeptides: biological and physiological properties," Toxicology, vol.
87, pp. 151-174, Feb. 1994.
[13] S. Freeman, T. Katan, and E. Shabi, "Characterization of Colletotrichum
gloeosporioides isolates from avocado and almond fruits with molecular
and pathogenicity tests," Appl. Environ. Microbiol., vol. 62, pp.1014-
1020, Mar. 1996.
[14] T. Stein. 2005. "Bacillus subtilis antibiotics: structure, syntheses and
specific functions," Mol. Microbiol, vol. 56, pp. 845-857, May 2005.
[15] Y. Kefialew, and A. Ayalew, "Postharvest biological control of
anthracnose (Colletotrichum gloeosporioides) on mango (Mangifera
indica)," Postharvest Biolo. Tec., vol. 50, pp. 8-11, Oct. 2008.
[16] Y. Liu, Z. Chen, T. B. Ng, J. Zhang, M. Zhou, F. Song, F. Lu, and Y.
Liu. "Bacisubin, an antifungal protein with rionuclease and
hemagglutinating activities from Bacillus subtilis strain B-916,"
Peptides, vol. 28, pp. 553-559, Mar. 2007.
[1] A. L. Senghor, W. J. Liang and W. C. Ho. "Integrated control of
Colletotrichum gloeosporioides on mango fruit in Taiwan by the
combination of Bacillus subtilis and fruit bagging," Biolcontrol Sci.
Techn., vol. 17, pp. 865-870, Sep. 2007.
[2] B. C. Sutton. "The genus Glomerella and its anamorph Colletotrichum"
in Colletotrichum: Biology, Pathology and Control, J. A. Bailey and M.
J. Jeger, Eds, Wallingford: CAB International, 1992, pp. 1-26.
[3] C.C. Lin, "Disease survey and integrated control of diseases on forcing
culture on wax-apple fruit trees (Translation Journals style)," Research
Bulletin of Kaosiung District Agricultural Improvement Station, vol. 13,
pp 20-29, Dec. 2001.
[4] D. Romero, A. de Vicente, J. L. Olmos, J. C. D├ívila, and A. Pérez-
García. "Effect of lipopeptides of antagonistic strains of Bacillus subtilis
on the morphology and ultrastructure of the cucurbit fungal pathogen
Podosphaera fusca," J. of Appl Microbiol, vol 103, pp. 969-976, Oct.
2007.
[5] E. A. B. Emmert and J. Handelsman. 1999. "Biocontrol of plant disease:
a (Gram-) positive perspective," FEMS Microbiol. Lett., vol. 171, pp. 1-
9, Feb. 1999.
[6] I. L. Shih, C. Y. Lin, J. Y. Wu, and C. Hsieh. "Production of antifungal
lipopeptide from Bacillus subtilis in submerged fermentation using
shake and fermenter," Korea J. Chem. Eng., vol. 26, pp. 1652-1661,
Nov. 2009.
[7] J.M. Waller, "Colletotrichum diseases of perennial and other cash crop
(Book style with paper title and editor)," in Colletotrichum: biology,
pathology and control, J.A. Bailey and M.J. Jeger, Eds, UK: Redwood
Press, 1992, pp. 167-185.
[8] L. Thimon, F.Peypoux , J. Wallach, and G. Michel. "Effect of the
lipopeptide antibiotic, iturin A, on morphology and membrane
ultrastructure of yeast cells," FEMS Microbiol. Lett., vol. 128, pp. 101-
106, May 1995.
[9] O. Ruangwong, C. I. Chang, A. L. Senghor and W. J. Liang.
"Identification of antifungal compound produced by Bacillus
subtilis LB5 with ability to control anthracnose disease caused
by Colletotrichum gloeosporioides," Afr. J. Microbiol. Res., vol. 6, pp
3732-3738, Apr. 2012.
[10] P. F. Cannon, P. D. Bridge, and E. Monte. "Linking the past, present and
future of Colletotrichum systematics," in Colletotrichum. Host
Specificity, Pathology and Host-Pathogen Interaction, D. Prusky, S.
Freeman, and M.B. Dickman, Eds, St Paul, Minnesota: APS Press,
2000, pp. 1-20.
[11] P. Leroux. "Modes of action of agrochemicals against plant pathogenic
organisms," C. R. Biol., vol. 326, pp. 9-2, Jan 2003.
[12] R. Maget-Dana, and F. Peypoux. "Iturins, a speial class of pore-forming
lipopeptides: biological and physiological properties," Toxicology, vol.
87, pp. 151-174, Feb. 1994.
[13] S. Freeman, T. Katan, and E. Shabi, "Characterization of Colletotrichum
gloeosporioides isolates from avocado and almond fruits with molecular
and pathogenicity tests," Appl. Environ. Microbiol., vol. 62, pp.1014-
1020, Mar. 1996.
[14] T. Stein. 2005. "Bacillus subtilis antibiotics: structure, syntheses and
specific functions," Mol. Microbiol, vol. 56, pp. 845-857, May 2005.
[15] Y. Kefialew, and A. Ayalew, "Postharvest biological control of
anthracnose (Colletotrichum gloeosporioides) on mango (Mangifera
indica)," Postharvest Biolo. Tec., vol. 50, pp. 8-11, Oct. 2008.
[16] Y. Liu, Z. Chen, T. B. Ng, J. Zhang, M. Zhou, F. Song, F. Lu, and Y.
Liu. "Bacisubin, an antifungal protein with rionuclease and
hemagglutinating activities from Bacillus subtilis strain B-916,"
Peptides, vol. 28, pp. 553-559, Mar. 2007.
@article{"International Journal of Medical, Medicine and Health Sciences:51003", author = "Onuma Ruangwong and Wen-Jinn Liang", title = "Effect of Crude Extract from Bacillus Subtilis LB5 Cultivated Broth on Conidial Germination of Colletotrichum Gloeosporioides", abstract = "Bacillus subtilis strain LB5 produced lipopeptide
antibiotic iturin A-2 in liquid medium. Crude extract
from cell-free supernatant of B. subtilis cultivated broth extracted
with n-butanol showed antifungal activity to conidial germination of
Colletotrichum gloeosporioides. The germination of conidia was
completely inhibited by crude extract. The ultrastructure of conidia
after treated with crude extract was found an accumulation of vesiclelike
material between cell wall and plasma membrane while this
accumulation was not observed in untreated and germinated conidia.
Besides, the cell wall was not affected by crude extract.", keywords = "Bacillus subtilis strain LB5, iturin A-2,
Colletotrichum gloeosporioides, TEM, vesicle-like material.", volume = "6", number = "12", pages = "634-5", }
