Abstract: Vermicomposting is the conversion of organic waste
into bio-fertilizers through the action of earthworm. This technology
is widely used for organic solid waste management. Waste corn pulp
blended with cow dung manure was vermicomposted over 30 days
using Eisenia fetida earthworms species. pH, temperature, moisture
content, and electrical conductivity were daily monitored. The
feedstock, vermicompost and vermiwash were analyzed for nutrient
composition. The average temperature and moisture content in the
vermi-reactor was 22.5°C and 42.5% respectively. The vermicompost
and vermiwash had an almost neutral pH whilst the electrical
conductivity was 21% higher in the vermicompost. The nitrogen and
potassium content was 57% and 79.6% richer in the vermicompost
respectively compared to the vermiwash. However, the vermiwash
was 84% richer in phosphorous as compared to vermicompost.
Furthermore, the vermiwash was 89.1% and 97.6% richer in Ca and
Mg respectively and was 97.8% richer in Na salts compared to the
vermicompost. The vermiwash also indicated a significantly higher
amount of micronutrients. Both bio-fertilizers were rich in nutrients
specification for fertilizers.
Abstract: Biodisel is a type of biofuel having similar properties of diesel fuel but lacks substances (undesirable emissions) such as sulfur, nitrogen and aromatic polycyclic. Upon filtration of waste oil, the biodiesel fuel was produced via carrying out transestrification reaction of triglycerides followed by conducting viscosity, density, flash point, cloud point, pour point and copper strip corrosion tests on the samples and comparing with EN14214 and ASTM 6751 standards and all results were found in the permitted limit. The highest yield of biodiesel production reaction was found 46.6435 g when Sodium Hydroxide catalyst in amount of 0.375g was employed, 44.2347 g when Sodium methoxide catalyst in amount of 0.5g was employed and 56.5124 g when acid sulfuric catalyst in amount of 1g was employed and 47.3290 g when two stage reaction was done.
Abstract: To decompose organochlorides by bioremediation, co-culture biohydrogen producer and dehalogenation microorganisms is a useful method. In this study, we combined these two characteristics from a biohydrogen producer, Rhodopseudomonas palustris, and a dehalogenation microorganism, Pseudomonas putida, to enchance halorespiration in R. palustris. The genes encoding cytochrome P450cam system (camC, camA, and camB) from P. putida were expressed in R. palustris with designated expression plasmid. All tested strains were cultured to log phase then presented pentachloroethane (PCA) in media. The vector control strain could degrade PCA about 78% after 16 hours, however, the cytochrome P450cam system expressed strain, CGA-camCAB, could completely degrade PCA in 12 hours. While taking chlorinated aromatic, 3-chlorobenzoate, as sole carbon source or present benzoate as co-substrate, CGA-camCAB presented faster growth rate than vector control strain.
Abstract: The distribution, enrichment, accumulation, and potential ecological risk of copper (Cu) in the surface sediments of northern Kaohsiung Harbor, Taiwan were investigated. Sediment samples from 12 locations of northern Kaohsiung Harbor were collected and characterized for Cu, aluminum, water content, organic matter, total nitrogen, total phosphorous, total grease and grain size. Results showed that the Cu concentrations varied from 6.9–244 mg/kg with an average of 109±66 mg/kg. The spatial distribution of Cu reveals that the Cu concentration is relatively high in the river mouth region, and gradually diminishes toward the harbor entrance region. This indicates that upstream industrial and municipal wastewater discharges along the river bank are major sources of Cu pollution. Results from the enrichment factor and geo-accumulation index analyses imply that the sediments collected from the river mouth can be characterized between moderate and moderately severe degree enrichment and between none to medium and moderate accumulation of Cu, respectively. However, results of potential ecological risk index indicate that the sediment has low ecological potential risk.
Abstract: The influence of axial magnetic field (B=0.48 T) on
the variation of ionization efficiency coefficient h and secondary
electron emission coefficient g with respect to reduced electric field
E/P is studied at a new range of plane-parallel electrode spacing (0<
d< 20 cm) and different nitrogen working pressure between 0.5-20
Pa. The axial magnetic field is produced from an inductive copper
coil of radius 5.6 cm. The experimental data of breakdown voltage is
adopted to estimate the mean Paschen curves at different working
features. The secondary electron emission coefficient is calculated
from the mean Paschen curve and used to determine the minimum
breakdown voltage. A reduction of discharge voltage of about 25% is
investigated by the applied of axial magnetic field. At high interelectrode
spacing, the effect of axial magnetic field becomes more
significant for the obtained values of h but it was less for the values
of g.
Abstract: Solid state fermentation of cassava peel with emphasis on protein enrichment using Trichoderma viride was evaluated. The effect of five variables: moisture content, pH, particle size (p), nitrogen source and incubation temperature; on the true protein and total sugars of cassava peel was investigated. The optimum fermentation period was established to be 8 days. Total sugars were 5-fold higher at pH 6 relative to pH 4 and 7-fold higher when cassava peels were fermented at 30oC relative to 25oC as well as using ammonium sulfate as the nitrogen source relative to urea or a combination of both. Total sugars ranged between 123.21mg/g at 50% initial moisture content to 374mg/g at 60% and from 190.59mg/g with particle size range of 2.00>p>1.41mm to 310.10mg/g with 4.00>p>3.35mm.True protein ranged from 229.70 mg/g at pH 4 to 284.05 mg/g at pH 6; from 200.87 mg/g with urea as nitrogen source and to 254.50mg/g with ammonium sulfate; from 213.82mg/g at 50% initial moisture content to 254.50mg/g at 60% moisture content, from 205.75mg/g in cassava peel with 5.6>p> 4.75mm to 268.30 in cassava peel with particle size 4.00>p>3.35mm, from 207.57mg/g at 25oC to 254.50mg/g at 30oC Cassava peel with particle size 4.00>p>3.35 mm and initial moisture content of 60% at pH 6.0, 30oC incubation temperature with ammonium sulfate (10g N / kg substrate) was most suitable for protein enrichment with Trichoderma viride. Crude protein increased from 4.21 % in unfermented cassava peel samples to 10.43 % in fermented samples.
Abstract: “Garbage enzyme", a fermentation product of kitchen waste, water and brown sugar, is claimed in the media as a multipurpose solution for household and agricultural uses. This study assesses the effects of dilutions (5% to 75%) of garbage enzyme in reducing pollutants in domestic wastewater. The pH of the garbage enzyme was found to be 3.5, BOD concentration about 150 mg/L. Test results showed that the garbage enzyme raised the wastewater-s BOD in proportion to its dilution due to its high organic content. For mixtures with more than 10% garbage enzyme, its pH remained acidic after the 5-day digestion period. However, it seems that ammonia nitrogen and phosphorus could be removed by the addition of the garbage enzyme. The most economic solution for removal of ammonia nitrogen and phosphorus was found to be 9%. Further tests are required to understand the removal mechanisms of the ammonia nitrogen and phosphorus.
Abstract: Eutrophication of surface water is one of the most
widespread environmental problems at present. Large number of
pilgrims and tourists visit sacred artificial tank known as “Brahma
Sarover” located at Kurukshetra, India to take holy dip and perform
religious ceremonies. The sources of pollutants include impurities in
feed water, mass bathing, religious offerings and windblown
particulate matter. Studies so far have focused mainly on assessing
water quality for bathing purpose by using physico-chemical and
bacteriological parameters. No effort has been made to assess nutrient
concentration and trophic status of the tank to take more appropriate
measures for improving water quality on long term basis. In the
present study, total nitrogen, total phosphorous and chlorophyll a
measurements have been done to assess the nutrient level and trophic
status of the tank. The results show presence of high concentration of
nutrients and Chlorophyll a indicating mesotrophic and eutrophic
state of the tank. Phosphorous has been observed as limiting nutrient
in the tank water.
Abstract: Three strains of Vibrio parahaemolyticus (690, BCRC
13023 and BCRC 13025) implicated in food poisoning outbreaks in
Taiwan were subjected to acid adaptation at pH 5.5 for 90 min. The
growth behaviors of acid-adapted and non-adapted V.
parahaemolyticus in the media supplemented with various nitrogen
and carbon sources were investigated. The effects of acid adaptation
on the thermostable direct hemolysin (TDH) secretion and fatty acid
profiles of V. parahaemolyticus were also examined. Results showed
that acid-adapted and non-adapted V. parahaemolyticus 690, BCRC
13023 and BCRC 13025 grew similarly in TSB-3% NaCl and basal
media supplemented with various carbon and nitrogen sources during
incubation period. Higher TDH secretion was noted with V.
parahaemolyticus 690 among the three strains. However, acid-adapted
strains produced less amounts of TDH than non-adapted strains when
they were grown in TSB-3% NaCl. Additionally, acid adaptation
increased the ratio of SFA/USFA in cells of V. parahaemolyticus
strains.
Abstract: This paper present some preliminary work on the
preparation and physicochemical caracterization of nanocomposite
MFI-alumina structures based on alumina hollow fibres. The fibers
are manufactured by a wet spinning process. α-alumina particles were
dispersed in a solution of polysulfone in NMP. The resulting slurry is
pressed through the annular gap of a spinneret into a precipitation
bath. The resulting green fibres are sintered. The mechanical strength
of the alumina hollow fibres is determined by a three-point-bending
test while the pore size is characterized by bubble-point testing. The
bending strength is in the range of 110 MPa while the average pore
size is 450 nm for an internal diameter of 1 mm and external diameter
of 1.7 mm. To characterize the MFI membranes various techniques
were used for physicochemical characterization of MFI–ceramic
hollow fibres membranes: The nitrogen adsorption, X-ray
diffractometry, scanning electron microscopy combined with X
emission microanalysis. Scanning Electron Microscopy (SEM) and
Energy Dispersive Microanalysis by the X-ray were used to observe
the morphology of the hollow fibre membranes (thickness,
infiltration into the carrier, defects, homogeneity). No surface film,
has been obtained, as observed by SEM and EDX analysis and
confirmed by high temperature variation of N2 and CO2 gas
permeances before cation exchange. Local analysis and characterise
(SEM and EDX) and overall (by ICP elemental analysis) were
conducted on two samples exchanged to determine the quantity and
distribution of the cation of cesium on the cross section fibre of the
zeolite between the cavities.
Abstract: Repeated additions of the unfertilized bacteria led to
increase the activity of Nitrogen-fixing bacteria in the root zone with
drip irrigation system compared to traditional manual vaccination to
increase the proportion of Nitrogen from 29% to 64%, and the
efficiency of adding Nitrogen fertilizer did not exceed 9.5% while
dropped to 4%, due to the amount of fertilizer added was not exceed
20kg N/h, and the second was the existence of a large amount of
available Nitrogen in the soil by fixation, while the efficiency of
irrigation system between 2.08 to 2.26 kg/m3.
Abstract: The fluorescent pseudomonad strain R81 is a root
colonizing rhizobacteria which promotes the growth of many plants
by various mechanisms. Its broth containing siderophore (ironchelating
compound) and 2,4- diacetyl phloroglucinol (DAPG) is
used for preparing bioinoculant formulations for agronomical
applications. Glycerol was found to be the best carbon source for
improved biomass production. Splitting of nitrogen source to NH4Cl
and urea had a stabilizing effect on pH during batch cultivation. Ltryptophan
at 0.5 % in the medium increased the siderophore
production to 850 mg/l. During batch cultivation of the strain in a
bioreactor, a maximum of 4 g/l of dry cell mass, 1.8 g/l of
siderophore and 20 mg/l of DAPG was achieved when glycerol was
15 g/l and C/N ratio was maintained at 12.5. In case of intermittent
feeding of fresh medium during fed-batch cultivation, the dry cell
mass was increased to 25 g/l with improved production of DAPG to
70 mg/l.
Abstract: Life is beautiful. But, it is decided by genes, environment and the individual and shattered by the natural and / or the invited problems. Most of the global rural helpless masses are struggling for their survival since; they are neglected in all aspects of life including health. Amidst a countless number of miserable diseases in man, diabetes is becoming a dreaded killer and ramifying the entire globe in a jet speed. Diabetes control continues as a Herculean task to the scientific community and the modern society in the 21st century also. T2DM is not pertaining to any age and it can develop even during the childhood. This multifactorial disease abruptly changes the activities of certain vital biomarkers in the present rural T2DM cases. A remarkable variation in the levels of biomarkers like AST, ALT, GGT, ALP, LDH, HbA1C, C- peptide, fasting sugar, post-prandial sugar, sodium, potassium, BUN, creatinine and insulin show the rampant nature of T2DM in this physically active rural agrarian community.
Abstract: A thirty Rahmani weaned male lambs of average body weight (27.28±1.40 kg) were randomly allotted to three similar groups, ten lambs in each, to study the benefit of commercial feed additives Tonilisat (Saccharomyces cerevisiae) and Roemin W2 (Lactobacillus acidophilus, Lactobacillus thermophilus, Bifidobacterium and Lactose) as growth promoters on lambs performance, digestibility, rumen activity and some blood constituents. The experiment lasted about 107 days. Three experimental groups were allotted as control group: received the basal ration, T1 group: received the basal ration supplemented with Tonilisat as (0.5kg/ ton concentrate feed mixture) and T2 group: received the basal ration supplemented with Roemin W2 (1kg/ ton concentrate feed mixture). Our study revealed that addition of Tonilisat significantly increased digestion coefficient of crude protein than that of the control group, Furthermore, the supplementation of Tonilisat or Roemin W2 increased (p
Abstract: Xanthan gum is one of the major commercial
biopolymers. Due to its excellent rheological properties xanthan gum
is used in many applications, mainly in food industry. Commercial
production of xanthan gum uses glucose as the carbon substrate;
consequently the price of xanthan production is high. One of the
ways to decrease xanthan price, is using cheaper substrate like
agricultural wastes. Iran is one of the biggest date producer countries.
However approximately 50% of date production is wasted annually.
The goal of this study is to produce xanthan gum from waste date
using Xanthomonas campestris PTCC1473 by submerged
fermentation. In this study the effect of three variables including
phosphor and nitrogen amount and agitation rate in three levels using
response surface methodology (RSM) has been studied. Results
achieved from statistical analysis Design Expert 7.0.0 software
showed that xanthan increased with increasing level of phosphor.
Low level of nitrogen leaded to higher xanthan production. Xanthan
amount, increasing agitation had positive influence. The statistical
model identified the optimum conditions nitrogen amount=3.15g/l,
phosphor amount=5.03 g/l and agitation=394.8 rpm for xanthan. To
model validation, experiments in optimum conditions for xanthan
gum were carried out. The mean of result for xanthan was 6.72±0.26.
The result was closed to the predicted value by using RSM.
Abstract: The main purpose of this research is the calculation of implicit prices of the environmental level of air quality in the city of Moscow on the basis of housing property prices. The database used contains records of approximately 20 thousand apartments and has been provided by a leading real estate agency operating in Russia. The explanatory variables include physical characteristics of the houses, environmental (industry emissions), neighbourhood sociodemographic and geographic data: GPS coordinates of each house. The hedonic regression results for ecological variables show «negative» prices while increasing the level of air contamination from such substances as carbon monoxide, nitrogen dioxide, sulphur dioxide, and particles (CO, NO2, SO2, TSP). The marginal willingness to pay for higher environmental quality is presented for linear and log-log models.
Abstract: The growth of the aquaculture industry has been
associated with negative environmental impacts through the
discharge of raw effluents into the adjacent receiving water bodies.
Macrophytes from natural saline lakes, which have adaptability to the
high salinity, can be suitable for saline effluent treatment. Eight
emergent species from natural saline area were planted in an
experimental gravel bed hydroponic mesocosm (GBH) which was
treated with effluent water from an intensive fish farm using
geothermal water. In order to examine the applicability of the
halophytes in treatment processes, we tested the relative efficacy of
total nitrogen (TN), total phosphorus (TP), potassium (K), sodium
(Na), magnesium (Mg) and calcium (Ca) removal for the saline
wastewater treatment. Four of the eight species, which were
Phragmites australis, Typha angustifolia, Glyceria maxima, Scirpus
lacustris spp. tabernaemontani could survive and contribute the
experimental treatment.
Abstract: Adsorption of methanol and ethanol over mesoporous
siliceous material are studied in the current paper. The pure
mesoporous silica is prepared using tetraethylorthosilicate (TEOS) as
silica source and dodecylamine as template at low pH. The prepared
material was characterized using nitrogen adsorption,nX-ray
diffraction (XRD) and scanning electron microscopy (SEM). The
adsorption kinetics of methanol and ethanol from aqueous solution
were studied over the prepared mesoporous silica material. The
percent removal of alcohol was calculated per unit mass of adsorbent
used. The 1st order model is found to be in agreement with both
adsorbates while the 2nd order model fit the adsorption of methanol
only.
Abstract: Gas hydrates form when a number of factors co-exist:
free water, hydrocarbon gas, cold temperatures and high pressures are typical of the near mud-line conditions in a deepwater drilling
operation. Subsequently, when drilling with water based muds, particularly on exploration wells, the risk of hydrate formation
associated with a gas influx is high. The consequences of gas hydrate
formation while drilling are severe, and as such, every effort should be made to ensure the risk of hydrate formation is either eliminated
or significantly reduced. Thermodynamic inhibitors are used to reduce the free water content of a drilling mud, and thus suppress the
hydrate formation temperature. Very little experimental work has
been performed by oil and gas research companies on the evaluation
of gas hydrate formation in a water-based drilling mud. The main
objective of this paper is to investigate the experimental gas hydrate
formation for a mixture of methane, carbon dioxide & nitrogen in a
water-based drilling mud with or without presence of different
concentrations of thermodynamic inhibitors including pure salt and a
combination of salt with methanol or ethylene glycol at different
concentrations in a static loop apparatus. The experiments were
performed using a static loop apparatus consisting of a 2.4307 cm
inside diameter and 800 cm long pipe. All experiments were conducted at 2200 psia. The temperature in the loop was decreased at
a rate of 3.33 °F/h from initial temperature of 80 °F.
Abstract: This study aims to screen out and to optimize the
major nutrients for maximum carotenoid production and
antioxidation characteristics by Rhodotorula rubra. It was found that
supplementary of 10 g/l glucose as carbon source, 1 g/l ammonium
sulfate as nitrogen source and 1 g/l yeast extract as growth factor in
the medium provided the better yield of carotenoid content of 30.39
μg/g cell dry weight the amount of antioxidation of Rhodotorula
rubra by DPPH, ABTS and MDA method were 1.463%, 34.21% and
34.09 μmol/l, respectively.