Process Optimization for Enhanced Production of Cell Biomass and Metabolites of Fluorescent Pseudomonad R81
The fluorescent pseudomonad strain R81 is a root
colonizing rhizobacteria which promotes the growth of many plants
by various mechanisms. Its broth containing siderophore (ironchelating
compound) and 2,4- diacetyl phloroglucinol (DAPG) is
used for preparing bioinoculant formulations for agronomical
applications. Glycerol was found to be the best carbon source for
improved biomass production. Splitting of nitrogen source to NH4Cl
and urea had a stabilizing effect on pH during batch cultivation. Ltryptophan
at 0.5 % in the medium increased the siderophore
production to 850 mg/l. During batch cultivation of the strain in a
bioreactor, a maximum of 4 g/l of dry cell mass, 1.8 g/l of
siderophore and 20 mg/l of DAPG was achieved when glycerol was
15 g/l and C/N ratio was maintained at 12.5. In case of intermittent
feeding of fresh medium during fed-batch cultivation, the dry cell
mass was increased to 25 g/l with improved production of DAPG to
70 mg/l.
[1] A. Sharma, B.N. Johri, A.K. Sharma, B.R. Glick, "Plant growthpromoting
bacterium pseudomonas sp. strain GRP3 influences iron
acquisition in mung bean (Vigna radiata L. Wilzeck)," Soil. Biol.
Biochem, vol. 35, pp 887-894, Mar. 2003.
[2] A.R. Podile, and G.K. Kishore, "Plant Growth Promoting
Rhizobacteria," in Plant-associated bacteria, 1st ed. S.S.
Gnanamanickam, Ed, Netherlands, Springer, 2006, pp 195-230.
[3] J. Leong, J, "Siderophores: their biochemistry and possible role in the
biocontrol of plant pathogen," Ann. Rev. Phytopathol, vol. 24, pp 187-
209, 1986.
[4] C. Voisard, C. Keel, D. Hass, and G. Defago, "Cyanide production by
Pseudomonas fluorescens suppress helps black root rot of tobacco under
gnotobiotic conditions," EMBO J, vol. 8, pp 351-358. 1989.
[5] R. Gaur, S. Noam, S. Kawaljeet, B.N. Johri, P. Rossi, and M. Aragno,
"Diacetylphloroglucinol producing pseudomonads do not influence AM
fungi in wheat rhizosphere," Curr. Sci, vol. 86, pp 453-457. Feb. 2004.
[6] D.J. O-Sullivan, and F. O-Gara, "Traits of fluorescent pseudomonas sp.
involved in suppression of plant root pathogens," Microbiol. Rev, vol.
56, pp 662-676, Dec.1992.
[7] D. Roesti, R. Gaur, B.N. Johri, G. Imfeld, S. Sharma, K. Kwaljeet, and
M. Aragno, "Plant growth stage, fertilizer management and bioinoculation
of arbuscular mycorrhizal fungi and plant growth promoting
rhizobacteria affect the rhizobacterial community structure in rain-fed
wheat fields," Soil. Biol. Biochem, vol. 38, pp 1111-1120, Jan. 2006.
[8] M. Aragno, and H.G. Schlegel, "The mesophilic hydrogen-oxidizing
(Knallgas) bacteria," in The Prokaryotes, A. Balows, H.G. Tr├╝per, M.
Dworkin, W. Harder, and K.H. Schleifer KH, Eds. Berlin Heidelberg
New York: Springer Verlag, 1991, pp. 344-384.
[9] J.M. Meyer, and M.A. Abdallah, "The florescent pigment of
pseudomonas fluorescens biosynthesis, purification and physicalchemical
properties," J Gen Microbiol, vol. 107, pp 319-328, April
1978.
[10] S.H. Bok, and A.L. Demain, "An improved colorimetric assay for
polyols," Anal. Biochem, vol. 81, pp.18-20, March 1977.
[11] S.H. Hua, T. Yoshida, Y. Meng, T. Kabashima, K. Ito, Y. Nishiya, Y.
Kawamura, and T. Yoshimoto, "Purification and characterization of
thermostable glycerol kinase from Thermus flavus," J. Ferment. Bioeng,
vol. 83, pp 328-332, Jan. 1997.
[12] D. Rachid, and B. Ahmed, "Effect of iron and growth inhibitors on
siderophores production by Pseudomonas fluorescens" African J.
Biotechnol, vol. 4, pp 697-702, July 2005.
[13] E. María, and Díaz de Villegas "Biotechnological production of
siderophores," in Microbial Siderophores, Series: Soil Biology, Vol. 12,
A. Varma, and S. Chincholkar, Eds, Berlin, Heidelberg: Springer Verlag,
2007, pp. 219-231.
[14] T. Yamane, and S. Shimizu, "Fed-batch techniques in microbial
processes," Adv. Biochem. Eng, vol. 30, pp 147-194. 1984.
[15] L. Yee, and H.W. Blanch, "Recombinant trypsin production in high cell
density fed-batch cultures in Escherichia coli," Biotechnol. Bioeng, vol.
41, pp 781-790, April 1993.
[1] A. Sharma, B.N. Johri, A.K. Sharma, B.R. Glick, "Plant growthpromoting
bacterium pseudomonas sp. strain GRP3 influences iron
acquisition in mung bean (Vigna radiata L. Wilzeck)," Soil. Biol.
Biochem, vol. 35, pp 887-894, Mar. 2003.
[2] A.R. Podile, and G.K. Kishore, "Plant Growth Promoting
Rhizobacteria," in Plant-associated bacteria, 1st ed. S.S.
Gnanamanickam, Ed, Netherlands, Springer, 2006, pp 195-230.
[3] J. Leong, J, "Siderophores: their biochemistry and possible role in the
biocontrol of plant pathogen," Ann. Rev. Phytopathol, vol. 24, pp 187-
209, 1986.
[4] C. Voisard, C. Keel, D. Hass, and G. Defago, "Cyanide production by
Pseudomonas fluorescens suppress helps black root rot of tobacco under
gnotobiotic conditions," EMBO J, vol. 8, pp 351-358. 1989.
[5] R. Gaur, S. Noam, S. Kawaljeet, B.N. Johri, P. Rossi, and M. Aragno,
"Diacetylphloroglucinol producing pseudomonads do not influence AM
fungi in wheat rhizosphere," Curr. Sci, vol. 86, pp 453-457. Feb. 2004.
[6] D.J. O-Sullivan, and F. O-Gara, "Traits of fluorescent pseudomonas sp.
involved in suppression of plant root pathogens," Microbiol. Rev, vol.
56, pp 662-676, Dec.1992.
[7] D. Roesti, R. Gaur, B.N. Johri, G. Imfeld, S. Sharma, K. Kwaljeet, and
M. Aragno, "Plant growth stage, fertilizer management and bioinoculation
of arbuscular mycorrhizal fungi and plant growth promoting
rhizobacteria affect the rhizobacterial community structure in rain-fed
wheat fields," Soil. Biol. Biochem, vol. 38, pp 1111-1120, Jan. 2006.
[8] M. Aragno, and H.G. Schlegel, "The mesophilic hydrogen-oxidizing
(Knallgas) bacteria," in The Prokaryotes, A. Balows, H.G. Tr├╝per, M.
Dworkin, W. Harder, and K.H. Schleifer KH, Eds. Berlin Heidelberg
New York: Springer Verlag, 1991, pp. 344-384.
[9] J.M. Meyer, and M.A. Abdallah, "The florescent pigment of
pseudomonas fluorescens biosynthesis, purification and physicalchemical
properties," J Gen Microbiol, vol. 107, pp 319-328, April
1978.
[10] S.H. Bok, and A.L. Demain, "An improved colorimetric assay for
polyols," Anal. Biochem, vol. 81, pp.18-20, March 1977.
[11] S.H. Hua, T. Yoshida, Y. Meng, T. Kabashima, K. Ito, Y. Nishiya, Y.
Kawamura, and T. Yoshimoto, "Purification and characterization of
thermostable glycerol kinase from Thermus flavus," J. Ferment. Bioeng,
vol. 83, pp 328-332, Jan. 1997.
[12] D. Rachid, and B. Ahmed, "Effect of iron and growth inhibitors on
siderophores production by Pseudomonas fluorescens" African J.
Biotechnol, vol. 4, pp 697-702, July 2005.
[13] E. María, and Díaz de Villegas "Biotechnological production of
siderophores," in Microbial Siderophores, Series: Soil Biology, Vol. 12,
A. Varma, and S. Chincholkar, Eds, Berlin, Heidelberg: Springer Verlag,
2007, pp. 219-231.
[14] T. Yamane, and S. Shimizu, "Fed-batch techniques in microbial
processes," Adv. Biochem. Eng, vol. 30, pp 147-194. 1984.
[15] L. Yee, and H.W. Blanch, "Recombinant trypsin production in high cell
density fed-batch cultures in Escherichia coli," Biotechnol. Bioeng, vol.
41, pp 781-790, April 1993.
@article{"International Journal of Biological, Life and Agricultural Sciences:55850", author = "M.V.R.K Sarma and Krishna Saharan and Lalit Kumar and Ashwani Gautam and Avhijeet Kapoor and Nishant Srivastava and Vikram Sahai and V.S Bisaria", title = "Process Optimization for Enhanced Production of Cell Biomass and Metabolites of Fluorescent Pseudomonad R81", abstract = "The fluorescent pseudomonad strain R81 is a root
colonizing rhizobacteria which promotes the growth of many plants
by various mechanisms. Its broth containing siderophore (ironchelating
compound) and 2,4- diacetyl phloroglucinol (DAPG) is
used for preparing bioinoculant formulations for agronomical
applications. Glycerol was found to be the best carbon source for
improved biomass production. Splitting of nitrogen source to NH4Cl
and urea had a stabilizing effect on pH during batch cultivation. Ltryptophan
at 0.5 % in the medium increased the siderophore
production to 850 mg/l. During batch cultivation of the strain in a
bioreactor, a maximum of 4 g/l of dry cell mass, 1.8 g/l of
siderophore and 20 mg/l of DAPG was achieved when glycerol was
15 g/l and C/N ratio was maintained at 12.5. In case of intermittent
feeding of fresh medium during fed-batch cultivation, the dry cell
mass was increased to 25 g/l with improved production of DAPG to
70 mg/l.", keywords = "Batch cultivation, Fed-batch cultivation, fluorescent pseudomonad, Metabolites", volume = "4", number = "5", pages = "294-5", }
