Abstract: This study describes a capillary-based device
integrated with the heating and cooling modules for polymerase chain
reaction (PCR). The device consists of the reaction
polytetrafluoroethylene (PTFE) capillary, the aluminum blocks, and is
equipped with two cartridge heaters, a thermoelectric (TE) cooler, a
fan, and some thermocouples for temperature control. The cartridge
heaters are placed into the heating blocks and maintained at two
different temperatures to achieve the denaturation and the extension
step. Some thermocouples inserted into the capillary are used to obtain
the transient temperature profiles of the reaction sample during
thermal cycles. A 483-bp DNA template is amplified successfully in
the designed system and the traditional thermal cycler. This work
should be interesting to persons involved in the high-temperature
based reactions and genomics or cell analysis.
Abstract: In this paper, a new proposed system for Persian
printed numeral characters recognition with emphasis on
representation and recognition stages is introduced. For the first time,
in Persian optical character recognition, geometrical central moments
as character image descriptor and fuzzy min-max neural network for
Persian numeral character recognition has been used. Set of different
experiments on binary images of regular, translated, rotated and
scaled Persian numeral characters has been done and variety of
results has been presented. The best result was 99.16% correct
recognition demonstrating geometrical central moments and fuzzy
min-max neural network are adequate for Persian printed numeral
character recognition.
Abstract: Vernonia divergens Benth., commonly known as
“Insulin Plant” (Fam: Asteraceae) is a potent sugar killer. Locally the
leaves of the plant, boiled in water are successfully administered to a
large number of diabetic patients. The present study evaluates the
putative anti-diabetic ingredients, isolated from the in vivo and in
vitro grown plantlets of V. divergens for their antimicrobial and
anticancer activities. Sterilized explants of nodal segments were
cultured on MS (Musashige and Skoog, 1962) medium in presence of
different combinations of hormones. Multiple shoots along with
bunch of roots were regenerated at 1mg l-1 BAP and 0.5 mg l-1 NAA.
Micro-plantlets were separated and sub-cultured on the double
strength (2X) of the above combination of hormones leading to
increased length of roots and shoots. These plantlets were
successfully transferred to soil and survived well in nature. The
ethanol extract of plantlets from both in vivo & in vitro sources were
prepared in soxhlet extractor and then concentrated to dryness under
reduced pressure in rotary evaporator. Thus obtainedconcentrated
extracts showed significant inhibitory activity against gram
negative bacteria like Escherichia coli and Pseudomonas
aeruginosa but no inhibition was found against gram positive
bacteria. Further, these ethanol extracts were screened for in vitro
percentage cytotoxicity at different time periods (24 h, 48 h and 72 h)
of different dilutions. The in vivo plant extract inhibited the growth of
EAC mouse cell lines in the range of 65, 66, 78, and 88% at 100, 50,
25 & 12.5μg mL-1 but at 72 h of treatment. In case of the extract of in
vitro origin, the inhibition was found against EAC cell lines even at
48h. During spectrophotometric scanning, the extracts exhibited
different maxima (ʎ) - four peaks in in vitro extracts as against single
in in vivo preparation suggesting the possible change in the nature of
ingredients during micropropagation through tissue culture
techniques.
Abstract: In order to make conventional implicit algorithm to be applicable in large scale parallel computers , an interface prediction and correction of discontinuous finite element method is presented to solve time-dependent neutron transport equations under 2-D cylindrical geometry. Domain decomposition is adopted in the computational domain.The numerical experiments show that our parallel algorithm with explicit prediction and implicit correction has good precision, parallelism and simplicity. Especially, it can reach perfect speedup even on hundreds of processors for large-scale problems.
Abstract: The paper provides a numerical investigation of the
entropy generation analysis due to natural convection in an inclined
square porous cavity. The coupled equations of mass, momentum,
energy and species conservation are solved using the Control Volume
Finite-Element Method. Effect of medium permeability and
inclination angle on entropy generation is analysed. It was found that
according to the Darcy number and the porous thermal Raleigh
number values, the entropy generation could be mainly due to heat
transfer or to fluid friction irreversibility and that entropy generation
reaches extremum values for specific inclination angles.
Abstract: This paper analyses the unsteady, two-dimensional
stagnation point flow of an incompressible viscous fluid over a flat
sheet when the flow is started impulsively from rest and at the same
time, the sheet is suddenly stretched in its own plane with a velocity
proportional to the distance from the stagnation point. The partial
differential equations governing the laminar boundary layer forced
convection flow are non-dimensionalised using semi-similar
transformations and then solved numerically using an implicit finitedifference
scheme known as the Keller-box method. Results
pertaining to the flow and heat transfer characteristics are computed
for all dimensionless time, uniformly valid in the whole spatial region
without any numerical difficulties. Analytical solutions are also
obtained for both small and large times, respectively representing the
initial unsteady and final steady state flow and heat transfer.
Numerical results indicate that the velocity ratio parameter is found
to have a significant effect on skin friction and heat transfer rate at
the surface. Furthermore, it is exposed that there is a smooth
transition from the initial unsteady state flow (small time solution) to
the final steady state (large time solution).
Abstract: A physically based, spatially-distributed water quality model is being developed to simulate spatial and temporal distributions of material transport in the Great Lakes Watersheds of the U.S. Multiple databases of meteorology, land use, topography, hydrography, soils, agricultural statistics, and water quality were used to estimate nonpoint source loading potential in the study watersheds. Animal manure production was computed from tabulations of animals by zip code area for the census years of 1987, 1992, 1997, and 2002. Relative chemical loadings for agricultural land use were calculated from fertilizer and pesticide estimates by crop for the same periods. Comparison of these estimates to the monitored total phosphorous load indicates that both point and nonpoint sources are major contributors to the total nutrient loads in the study watersheds, with nonpoint sources being the largest contributor, particularly in the rural watersheds. These estimates are used as the input to the distributed water quality model for simulating pollutant transport through surface and subsurface processes to Great Lakes waters. Visualization and GIS interfaces are developed to visualize the spatial and temporal distribution of the pollutant transport in support of water management programs.
Abstract: In this paper we propose a new knowledge model using
the Dempster-Shafer-s evidence theory for image segmentation and
fusion. The proposed method is composed essentially of two steps.
First, mass distributions in Dempster-Shafer theory are obtained from
the membership degrees of each pixel covering the three image
components (R, G and B). Each membership-s degree is determined by
applying Fuzzy C-Means (FCM) clustering to the gray levels of the
three images. Second, the fusion process consists in defining three
discernment frames which are associated with the three images to be
fused, and then combining them to form a new frame of discernment.
The strategy used to define mass distributions in the combined
framework is discussed in detail. The proposed fusion method is
illustrated in the context of image segmentation. Experimental
investigations and comparative studies with the other previous methods
are carried out showing thus the robustness and superiority of the
proposed method in terms of image segmentation.
Abstract: Colored Petri Nets (CPN) are very known kind of
high level Petri nets. With sound and complete semantics, rewriting
logic is one of very powerful logics in description and verification of
non-deterministic concurrent systems. Recently, CPN semantics are
defined in terms of rewriting logic, allowing us to built models by
formal reasoning. In this paper, we propose an automatic translation
of CPN to the rewriting logic language Maude. This tool allows
graphical editing and simulating CPN. The tool allows the user
drawing a CPN graphically and automatic translating the graphical
representation of the drawn CPN to Maude specification. Then,
Maude language is used to perform the simulation of the resulted
Maude specification. It is the first rewriting logic based environment
for this category of Petri Nets.
Abstract: High-frequency (HF) communications have been used by military organizations for more than 90 years. The opportunity of very long range communications without the need for advanced equipment makes HF a convenient and inexpensive alternative of satellite communications. Besides the advantages, voice and data transmission over HF is a challenging task, because the HF channel generally suffers from Doppler shift and spread, multi-path, cochannel interference, and many other sources of noise. In constructing an HF data modem, all these effects must be taken into account. STANAG 4539 is a NATO standard for high-speed data transmission over HF. It allows data rates up to 12800 bps over an HF channel of 3 kHz. In this work, an efficient implementation of STANAG 4539 on a single Texas Instruments- TMS320C6747 DSP chip is described. The state-of-the-art algorithms used in the receiver and the efficiency of the implementation enables real-time high-speed data / digitized voice transmission over poor HF channels.
Abstract: Liposomal magnetofection is a simple, highly efficient
technology for cell transfection, demonstrating better outcome than a
number of other common gene delivery methods. However,
aggregate complexes distribution over the cell surface is non-uniform
due to the gradient of the permanent magnetic field. The aim of this
study was to estimate the efficiency of liposomal magnetofection for
prostate carcinoma PC3 cell line using newly designed device,
“DynaFECTOR", ensuring magnetofection in a dynamic gradient
magnetic field. Liposomal magnetofection in a dynamic gradient
magnetic field demonstrated the highest transfection efficiency for
PC3 cells – it increased for 21% in comparison with liposomal
magnetofection and for 42% in comparison with lipofection alone.
The optimal incubation time under dynamic magnetic field for PC3
cell line was 5 minutes and the optimal rotation frequency of
magnets – 5 rpm. The new approach also revealed lower cytotoxic
effect to cells than liposomal magnetofection.
Abstract: Gamboge disorder (GD) or fruit damage by the yellow sap is a major problem in mangosteen. Mangosteen plants varied in the level of GD, from very low or non GD to low, moderate and high GD. However it was difficult to differentiate between GD and non GD plants because evaluation of the disorder is strongly influenced by environment. In this study we investigated the usefulness of primer designed from bioinformatics related to cell wall strength, termed as MCWS, to predict GD. Plant materials used were 28 mangosteen plants selected based on percentage of GD categorized as high, moderate, low and very low or non GD. The result showed that the specific DNA fragments were absent in the high GD accessions. The MCWS marker suggests as a novel polymorphic marker for GD in mangosteen as well as a marker for detect variability in mangosteen as apomictic plant.
Abstract: Two novel hydrodesulfurization (HDS) catalysts:
FeN4/C and FeN2/C, were prepared using an impregnation-pyrolysis
method. The two materials were investigated as catalysts for
hydrodesulfurization (HDS) and hydrodearomitization (HDA) of
model compounds. The turnover frequency of the two FeN catalysts
is comparable to (FeN4/C) or even higher (FeN2/C) than that of
MoNi/Al2O3. The FeN4/C catalyst also exhibited catalytic activity
toward HDA.
Abstract: Determination of wellbore problems during a
production/injection process might be evaluated thorough
temperature log analysis. Other applications of this kind of log
analysis may also include evaluation of fluid distribution analysis
along the wellbore and identification of anomalies encountered
during production/injection process. While the accuracy of such
prediction is paramount, the common method of determination of a
wellbore temperature log includes use of steady-state energy balance
equations, which hardly describe the real conditions as observed in
typical oil and gas flowing wells during production operation; and
thus increase level of uncertainties. In this study, a practical method
has been proposed through development of a simplified semianalytical
model to apply for predicting temperature profile along the
wellbore. The developed model includes an overall heat transfer
coefficient accounting all modes of heat transferring mechanism,
which has been focused on the prediction of a temperature profile as
a function of depth for the injection/production wells. The model has
been validated with the results obtained from numerical simulation.
Abstract: The present paper concerns with the influence of fiber
packing on the transverse plastic properties of metal matrix
composites. A micromechanical modeling procedure is used to
predict the effective mechanical properties of composite materials at
large tensile and compressive deformations. Microstructure is
represented by a repeating unit cell (RUC). Two fiber arrays are
considered including ideal square fiber packing and random fiber
packing defined by random sequential algorithm. The
micromechanical modeling procedure is implemented for
graphite/aluminum metal matrix composite in which the
reinforcement behaves as elastic, isotropic solids and the matrix is
modeled as an isotropic elastic-plastic solid following the von Mises
criterion with isotropic hardening and the Ramberg-Osgood
relationship between equivalent true stress and logarithmic strain.
The deformation is increased to a considerable value to evaluate both
elastic and plastic behaviors of metal matrix composites. The yields
strength and true elastic-plastic stress are determined for
graphite/aluminum composites.
Abstract: In this study, synthesis of biomemitic patterned nano
hydroxyapatite-starch biocomposites using different concentration of
starch to evaluate effect of polymer alteration on biocomposites
structural properties has been reported. Formation of hydroxyapatite
nano particles was confirmed by X-ray diffraction (XRD) and Fourier
transform infrared spectroscopy (FT-IR). Size and morphology of the
samples were characterized using scanning and transmission electron
microscopy (SEM and TEM). It seems that by increasing starch
content, the more active site of polymer (oxygen atoms) can be
provided for interaction with Ca2+ followed by phosphate and
hydroxyl group.
Abstract: Opportunistic network is a kind of Delay Tolerant Networks (DTN) where the nodes in this network come into contact with each other opportunistically and communicate wirelessly and, an end-to-end path between source and destination may have never existed, and disconnection and reconnection is common in the network. In such a network, because of the nature of opportunistic network, perhaps there is no a complete path from source to destination for most of the time and even if there is a path; the path can be very unstable and may change or break quickly. Therefore, routing is one of the main challenges in this environment and, in order to make communication possible in an opportunistic network, the intermediate nodes have to play important role in the opportunistic routing protocols. In this paper we proposed an Adaptive Fuzzy Routing in opportunistic network (AFRON). This protocol is using the simple parameters as input parameters to find the path to the destination node. Using Message Transmission Count, Message Size and Time To Live parameters as input fuzzy to increase delivery ratio and decrease the buffer consumption in the all nodes of network.
Abstract: We numerically study the three-dimensional
magnetohydrodynamics (MHD) stability of oscillatory natural
convection flow in a rectangular cavity, with free top surface, filled
with a liquid metal, having an aspect ratio equal to A=L/H=5, and
subjected to a transversal temperature gradient and a uniform
magnetic field oriented in x and z directions. The finite volume
method was used in order to solve the equations of continuity,
momentum, energy, and potential. The stability diagram obtained in
this study highlights the dependence of the critical value of the
Grashof number Grcrit , with the increase of the Hartmann number
Ha for two orientations of the magnetic field. This study confirms
the possibility of stabilization of a liquid metal flow in natural
convection by application of a magnetic field and shows that the
flow stability is more important when the direction of magnetic field
is longitudinal than when the direction is transversal.
Abstract: Recognizing behavioral patterns of financial markets
is essential for traders. Japanese candlestick chart is a common tool to
visualize and analyze such patterns in an economic time series. Since
the world was introduced to Japanese candlestick charting, traders
saw how combining this tool with intelligent technical approaches
creates a powerful formula for the savvy investors.
This paper propose a generalization to box counting method of
Grassberger-Procaccia, which is based on computing the correlation
dimension of Japanese candlesticks instead commonly used 'close'
points. The results of this method applied on several foreign
exchange rates vs. IRR (Iranian Rial). Satisfactorily show lower
chaotic dimension of Japanese candlesticks series than regular
Grassberger-Procaccia method applied merely on close points of
these same candles. This means there is some valuable information
inside candlesticks.
Abstract: Food and fibre production in arid and semi-arid regions has emerged as one of the major challenges for various socio-economic and political reasons such as the food security and self-sufficiency. Productive use of the renewable water resources has risen on top ofthe decision-making agenda. For this reason, efficient operation and maintenance of modern irrigation and drainage schemes become part and parcel and indispensible reality in agricultural policy making arena. The aim of this paper is to investigate the complexity of operating and maintaining such schemes, mainly focussing on challenges which enhance and opportunities that impedsustainable food and fibre production. The methodology involved using secondary data complemented byroutine observations and stakeholders views on issues that influence the O&M in the Dez command area. The SPSS program was used as an analytical framework for data analysis and interpretation.Results indicate poor application efficiency in most croplands, much of which is attributed to deficient operation of conveyance and distribution canals. These in turn, are reportedly linked to inadequate maintenance of the pumping stations and hydraulic structures like turnouts,flumes and other control systems particularly in the secondary and tertiary canals. Results show that the aforementioned deficiencies have been the major impediment to establishing regular flow toward the farm gates which subsequently undermine application efficiency and tillage operationsat farm level. Results further show that accumulative impact of such deficiencies has been the major causes of poorcrop yield and quality that deem production system in these croplands uneconomic. Results further show that the present state might undermine the sustainability of agricultural system in the command area. The overall conclusion being that present water management is unlikely to be responsive to challenges that the sector faces. And in the absence of coherent measures to shift the status quo situation in favour of more productive resource use, it would be hard to fulfil the objectives of the National Economic and Socio-cultural Development Plans.