PTFE Capillary-Based DNA Amplification within an Oscillatory Thermal Cycling Device

This study describes a capillary-based device
integrated with the heating and cooling modules for polymerase chain
reaction (PCR). The device consists of the reaction
polytetrafluoroethylene (PTFE) capillary, the aluminum blocks, and is
equipped with two cartridge heaters, a thermoelectric (TE) cooler, a
fan, and some thermocouples for temperature control. The cartridge
heaters are placed into the heating blocks and maintained at two
different temperatures to achieve the denaturation and the extension
step. Some thermocouples inserted into the capillary are used to obtain
the transient temperature profiles of the reaction sample during
thermal cycles. A 483-bp DNA template is amplified successfully in
the designed system and the traditional thermal cycler. This work
should be interesting to persons involved in the high-temperature
based reactions and genomics or cell analysis.

• Jyh J. Chen
• Fu H. Yang
• Ming H. Liao

• Polymerase chain reaction
• thermal cycles
• capillary
• TE cooler.

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