Abstract: Liposomal magnetofection is a simple, highly efficient
technology for cell transfection, demonstrating better outcome than a
number of other common gene delivery methods. However,
aggregate complexes distribution over the cell surface is non-uniform
due to the gradient of the permanent magnetic field. The aim of this
study was to estimate the efficiency of liposomal magnetofection for
prostate carcinoma PC3 cell line using newly designed device,
“DynaFECTOR", ensuring magnetofection in a dynamic gradient
magnetic field. Liposomal magnetofection in a dynamic gradient
magnetic field demonstrated the highest transfection efficiency for
PC3 cells – it increased for 21% in comparison with liposomal
magnetofection and for 42% in comparison with lipofection alone.
The optimal incubation time under dynamic magnetic field for PC3
cell line was 5 minutes and the optimal rotation frequency of
magnets – 5 rpm. The new approach also revealed lower cytotoxic
effect to cells than liposomal magnetofection.
Abstract: Direct fermentation of 226 white rose tapioca stem to
ethanol by Fusarium oxysporum was studied in a batch reactor.
Fermentation of ethanol can be achieved by sequential pretreatment
using dilute acid and dilute alkali solutions using 100 mesh tapioca
stem particles. The quantitative effects of substrate concentration, pH
and temperature on ethanol concentration were optimized using a full
factorial central composite design experiment. The optimum process
conditions were then obtained using response surface methodology.
The quadratic model indicated that substrate concentration of 33g/l,
pH 5.52 and a temperature of 30.13oC were found to be optimum for
maximum ethanol concentration of 8.64g/l. The predicted optimum
process conditions obtained using response surface methodology was
verified through confirmatory experiments. Leudeking-piret model
was used to study the product formation kinetics for the production
of ethanol and the model parameters were evaluated using
experimental data.
Abstract: The importance for manipulating an incorporated
scaffold and directing cell behaviors is well appreciated for tissue
engineering. Here, we developed newly nano-topographic oxidized
silicon nanosponges capable of being various chemical modifications
to provide much insight into the fundamental biology of how cells
interact with their surrounding environment in vitro. A wet etching
technique is exerted to allow us fabricated the silicon nanosponges in a
high-throughput manner. Furthermore, various organo-silane
chemicals enabled self-assembled on the surfaces by vapor deposition.
We have found that Chinese hamster ovary (CHO) cells displayed
certain distinguishable morphogenesis, adherent responses, and
biochemical properties while cultured on these chemical modified
nano-topographic structures in compared with the planar oxidized
silicon counterparts, indicating that cell behaviors can be influenced
by certain physical characteristic derived from nano-topography in
addition to the hydrophobicity of contact surfaces crucial for cell
adhesion and spreading. Of particular, there were predominant
nano-actin punches and slender protrusions formed while cells were
cultured on the nano-topographic structures. This study shed potential
applications of these nano-topographic biomaterials for controlling
cell development in tissue engineering or basic cell biology research.
Abstract: Electrochemical-oxidation of Reactive Black-5 (RB- 5) was conducted for degradation using DSA type Ti/RuO2-SnO2- Sb2O5 electrode. In the study, for electro-oxidation, electrode was indigenously fabricated in laboratory using titanium as substrate. This substrate was coated using different metal oxides RuO2, Sb2O5 and SnO2 by thermal decomposition method. Laboratory scale batch reactor was used for degradation and decolorization studies at pH 2, 7 and 11. Current density (50mA/cm2) and distance between electrodes (8mm) were kept constant for all experiments. Under identical conditions, removal of color, COD and TOC at initial pH 2 was 99.40%, 55% and 37% respectively for initial concentration of 100 mg/L RB-5. Surface morphology and composition of the fabricated electrode coatings were characterized using scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX) respectively. Coating microstructure was analyzed by X-ray diffraction (XRD). Results of this study further revealed that almost 90% of oxidation occurred within 5-10 minutes.
Abstract: The effect of notch depth on the elastic new strainconcentration
factor (SNCF) of rectangular bars with single edge Unotch
under combined loading is studied here. The finite element
method (FEM) and super position technique are used in the current
study. This new SNCF under combined loading of static tension and
pure bending has been defined under triaxial stress state. The
employed specimens have constant gross thickness of 16.7 mm and
net section thickness varied to give net-to-gross thickness ratio ho/Ho
from 0.2 to 0.95. The results indicated that the elastic SNCF for
combined loading increases with increasing notch depth up to ho/Ho =
0.7 and sharply decreases with increasing notch depth. It is also
indicated that the elastic SNCF of combined loading is greater than
that of pure bending and less than that of the static tension for 0.2 ≤
ho/Ho ≤ 0.7. However, the elastic SNCF of combined loading is the
elastic SNCF for static tension and less than that of pure bending for
shallow notches (i.e. 0.8 ≤ ho/Ho ≤ 0.95).
Abstract: In this work, statistical experimental design was
applied for the optimization of medium constituents for Gentamicin
production by Micromsonospora echinospora subs pallida (MTCC
708) in a batch reactor and the results are compared with the ANN
predicted values. By central composite design, 50 experiments are
carried out for five test variables: Starch, Soya bean meal, K2HPO4,
CaCO3 and FeSO4. The optimum condition was found to be: Starch
(8.9,g/L), Soya bean meal (3.3 g/L), K2HPO4 (0.8 g/L), CaCO3 (4
g/L) and FeSO4 (0.03 g/L). At these optimized conditions, the yield
of gentamicin was found to be 1020 mg/L. The R2 values were found
to be 1 for ANN training set, 0.9953 for ANN test set, and 0.9286 for
RSM.
Abstract: This paper presents an information retrieval model on
XML documents based on tree matching. Queries and documents are
represented by extended trees. An extended tree is built starting from
the original tree, with additional weighted virtual links between each
node and its indirect descendants allowing to directly reach each
descendant. Therefore only one level separates between each node
and its indirect descendants. This allows to compare the user query
and the document with flexibility and with respect to the structural
constraints of the query. The content of each node is very important to
decide weither a document element is relevant or not, thus the content
should be taken into account in the retrieval process. We separate
between the structure-based and the content-based retrieval processes.
The content-based score of each node is commonly based on the
well-known Tf × Idf criteria. In this paper, we compare between
this criteria and another one we call Tf × Ief. The comparison
is based on some experiments into a dataset provided by INEX1 to
show the effectiveness of our approach on one hand and those of
both weighting functions on the other.
Abstract: The efficacy of the separate mixing of four tropical spicy and medicinal plant products: Dennettia tripetala Baker (pepper fruit), Eugenia aromatica Hook (clove), Piper guineense (Schum and Thonn) (black pepper) and Monodora myristica (Dunal) (African nut-meg) with a household vegetable oil was evaluated under tropical storage conditions for the control and reproductive performance of Dermestes maculatus (De Geer) (hide beetle) and Necroba rufipes (De Geer) (copra beetle) on African catfish, Clarias gariepinus (Burchell). Each of the plant materials was pulverized into powder and applied as a mix of 1ml of oil and plant powder at 2.5, 5.0, 7.5 and 10.0g per 100g of dried fish, and allowed to dry for 6h. Each of the four oil-mixed powder treatments evoked significant (P < 05) mortalities of the two insects compared with the control (oil only) at 1, 3 and 7 days post treatment. The oil-powder mixture dosages did not prevent insect egg hatchability but while the emergent larvae on the treated samples died, the emergent larvae in the control survived into adults. The application of oil-mixed powders effectively suppressed the emergence of the larvae of the beetles. Similarly, each of the oil-powder mixtures significantly reduced weight loss in smoked fish that were exposed to D. maculatus and N. rufipes when compared to the control (P < 05). The results of this study suggest that the plant powders rather than the domestic oil demonstrated protective ability against the fish beetles and confirm the efficacy of the plant products as pest control agents.
Abstract: This paper deals with the analysis of active constrained layer damping (ACLD) of doubly curved laminated composite shells using active fiber composite (AFC) materials. The constraining layer of the ACLD treatment has been considered to be made of the AFC materials. A three dimensional energy based finite element model of the smart doubly curved laminated composite shell integrated with a patch of such ACLD treatment has been developed to demonstrate the performance of the patch on enhancing the damping characteristics of the doubly curved laminated composite shells. Particular emphasis has been placed on studying the effect of variation of piezoelectric fiber orientation angle in the constraining AFC layer on the control authority of the ACLD patch.
Abstract: The fault current levels through the electric devices
have a significant impact on failure probability. New fault current
results in exceeding the rated capacity of circuit breaker and switching
equipments and changes operation characteristic of overcurrent relay.
In order to solve these problems, SFCL (Superconducting Fault
Current Limiter) has rising as one of new alternatives so as to improve
these problems. A fault current reduction differs depending on
installed location. Therefore, a location of SFCL is very important.
Also, SFCL decreases the fault current, and it prevents surrounding
protective devices to be exposed to fault current, it then will bring a
change of reliability. In this paper, we propose method which
determines the optimal location when SFCL is installed in power
system. In addition, the reliability about the power system which
SFCL was installed is evaluated. The efficiency and effectiveness of
this method are also shown by numerical examples and the reliability
indices are evaluated in this study at each load points. These results
show a reliability change of a system when SFCL was installed.
Abstract: Scheduling algorithm is a key technology in satellite
switching system with input-buffer. In this paper, a new scheduling
algorithm and its realization are proposed. Based on Crossbar
switching fabric, the algorithm adopts serial scheduling strategy and
adjusts the output port arbitrating strategy for the better equity of every
port. Consequently, it increases the matching probability. The
algorithm can greatly reduce the scheduling delay and cell loss rate.
The analysis and simulation results by OPNET show that the proposed
algorithm has the better performance than others in average delay and
cell loss rate, and has the equivalent complexity. On the basis of these
results, the hardware realization and simulation based on FPGA are
completed, which validate the feasibility of the new scheduling
algorithm.
Abstract: The early diagnostic decision making in industrial processes is absolutely necessary to produce high quality final products. It helps to provide early warning for a special event in a process, and finding its assignable cause can be obtained. This work presents a hybrid diagnostic schmes for batch processes. Nonlinear representation of raw process data is combined with classification tree techniques. The nonlinear kernel-based dimension reduction is executed for nonlinear classification decision boundaries for fault classes. In order to enhance diagnosis performance for batch processes, filtering of the data is performed to get rid of the irrelevant information of the process data. For the diagnosis performance of several representation, filtering, and future observation estimation methods, four diagnostic schemes are evaluated. In this work, the performance of the presented diagnosis schemes is demonstrated using batch process data.
Abstract: Steganography, derived from Greek, literally means
“covered writing". It includes a vast array of secret communications
methods that conceal the message-s very existence. These methods
include invisible inks, microdots, character arrangement, digital
signatures, covert channels, and spread spectrum communications.
This paper proposes a new improved version of Least Significant Bit
(LSB) method. The approach proposed is simple for implementation
when compared to Pixel value Differencing (PVD) method and yet
achieves a High embedding capacity and imperceptibility. The
proposed method can also be applied to 24 bit color images and
achieve embedding capacity much higher than PVD.
Abstract: The study area receives a wide variety of wastes
generated by municipalities and the industries like paints and
pigments, metal processing industries, thermal power plants electroprocessing
industries etc. The Physico-chemical and structural
investigation of water from river Pandu indicated high level of
chlorides and calcium which made the water unsuitable for human
use. Algae like Cyclotella fumida, Asterionella Formosa,
Cladophora glomerata, Pediastrum simplex, Scenedesmus bijuga,
Cladophora glomerata were the dominant pollution tolerant species
recorded under these conditions. The sensitive and less abundant
species of algae included Spirogyra sps., Merismopedia sps. The
predominance colonies of Zygnema sps, Phormidium sps,
Mycrocystis aeruginosa, Merismopedia minima, Pandorina morum,
seems to correlate with high organic contents of Pandu river water.
This study assumes significance as some algae can be used as
bioindicators of water pollution and algal floral of a municipal drain
carrying waste effluents from industrial area Kanpur and discharge
them into the river Pandu flowing onto southern outskirts of Kanpur
city.
Abstract: Von Willebrand-s disease is the most common
inherited bleeding disorder in humans, it
caused by qualitative abnormalities of the von Willebrand factor
(vWF). Our objective is to determine the prevalence of this disease at
part of the Algerian population in the East and the South by a
biological diagnosis based on specific biological tests (automated
platelet count, the bleeding time (TS), the time of cephalin + activator
(TCA), measure of the prothrombin rate (TP), vWF rate and factor
VIII rate, Molecular electrophoresis of vWF multimers in agarose gel
in the presence of SDS). Four patients of type III or severe
Willebrand-s disease were found on 200 suspect cases. All cases are
showed a deficit in vWF rate (< 5%), and factor VIII (P
Abstract: K-Modes is an extension of K-Means clustering algorithm, developed to cluster the categorical data, where the mean is replaced by the mode. The similarity measure proposed by Huang is the simple matching or mismatching measure. Weight of attribute values contribute much in clustering; thus in this paper we propose a new weighted dissimilarity measure for K-Modes, based on the ratio of frequency of attribute values in the cluster and in the data set. The new weighted measure is experimented with the data sets obtained from the UCI data repository. The results are compared with K-Modes and K-representative, which show that the new measure generates clusters with high purity.
Abstract: The cost of developing the software from scratch can
be saved by identifying and extracting the reusable components from
already developed and existing software systems or legacy systems
[6]. But the issue of how to identify reusable components from
existing systems has remained relatively unexplored. We have used
metric based approach for characterizing a software module. In this
present work, the metrics McCabe-s Cyclometric Complexity
Measure for Complexity measurement, Regularity Metric, Halstead
Software Science Indicator for Volume indication, Reuse Frequency
metric and Coupling Metric values of the software component are
used as input attributes to the different types of Neural Network
system and reusability of the software component is calculated. The
results are recorded in terms of Accuracy, Mean Absolute Error
(MAE) and Root Mean Squared Error (RMSE).
Abstract: This paper presents a modified version of the
maximum urgency first scheduling algorithm. The maximum
urgency algorithm combines the advantages of fixed and dynamic
scheduling to provide the dynamically changing systems with
flexible scheduling. This algorithm, however, has a major
shortcoming due to its scheduling mechanism which may cause a
critical task to fail. The modified maximum urgency first scheduling
algorithm resolves the mentioned problem. In this paper, we propose
two possible implementations for this algorithm by using either
earliest deadline first or modified least laxity first algorithms for
calculating the dynamic priorities. These two approaches are
compared together by simulating the two algorithms. The earliest
deadline first algorithm as the preferred implementation is then
recommended. Afterwards, we make a comparison between our
proposed algorithm and maximum urgency first algorithm using
simulation and results are presented. It is shown that modified
maximum urgency first is superior to maximum urgency first, since it
usually has less task preemption and hence, less related overhead. It
also leads to less failed non-critical tasks in overloaded situations.
Abstract: The purpose of this study was to explore the
demographic differences of international tourists according to three
main factors, including the value of time, shopping behavior and
shopping motivation. The Chatuchak Weekend Market is known as
one of the biggest weekend markets in the world. Too little academic
studies had been conducted in this area of weekend market, despite its
growth and continuous development. In general, both domestic
visitors and international tourists are attracted to the perception of
cheap and bargaining prices the weekend market. However, systematic
research study can provide reliable understanding of the perception of
the visitors.
This study focused on the group of international tourists who visited
the market and aimed to provide better insights based on the
differences in their demographic factors. Findings indicated that
several differences in value of time, shopping behavior, and shopping
motivation were identified by gender, income and age. Research
implications and directions for further studies were discussed.
Abstract: This paper proposes a resonant dc link in PWM ac
chopper. This can solve the spike problems and also reduce the
switching loss. The configuration and PWM pattern of the proposed
technique are presented. The simulation results are used to confirm
the theory.