Optimization of Gentamicin Production: Comparison of ANN and RSM Techniques
In this work, statistical experimental design was
applied for the optimization of medium constituents for Gentamicin
production by Micromsonospora echinospora subs pallida (MTCC
708) in a batch reactor and the results are compared with the ANN
predicted values. By central composite design, 50 experiments are
carried out for five test variables: Starch, Soya bean meal, K2HPO4,
CaCO3 and FeSO4. The optimum condition was found to be: Starch
(8.9,g/L), Soya bean meal (3.3 g/L), K2HPO4 (0.8 g/L), CaCO3 (4
g/L) and FeSO4 (0.03 g/L). At these optimized conditions, the yield
of gentamicin was found to be 1020 mg/L. The R2 values were found
to be 1 for ANN training set, 0.9953 for ANN test set, and 0.9286 for
RSM.
[1] M. J. Weinstein, G.M. Luedemann, E.M. Oden, G.H. Wagman, J.P.
Rosselet, J.A. Marquez, C.T. Coniglio, W. Charney, H.L. Herzog, and J.
Black, "Gentamicin, new antibiotic complex from Micromonospora", J.
Med. Chem., vol. 6, 1963, pp. 463-464.
[2] J. Chu, S. Zhang, Y. Zhuang, J. Chen, and Y. Li, "Factors affecting the
biosynthesis and secretion of gentamicin", Process Biochem., vol.38,
2002, pp. 815-820.
[3] J. Chu, W. Niu, S. Zhang, Y. Zhuang, H. Hu, and Y. Li, "Effect of metal
ions on the binding of gentamicin to the peptidoglycan of
Micromonospora echinospora, Process Biochem, vol.39, 2004, pp.1145-
1150.
[4] M. Himabindu, R. Potumarthi, and A. Kitty, "Enhancement of
gentamicin production by mutugenesis and non nutritional stress
conditions in Micromonospora echinospora". Process Biochem, vol.42,
2007, pp. 1352-1356.
[5] M. Himabindu, P. Ravichandra, and J. Annapurna, "Evaluation of
immobilization conditions for enhanced production of gentamicin in
repeated batch operation by micromonospora echinospora"Int.J.of
chemical Reaction Engineering, vol.5, 2007, pp. 1-13.
[6] M. Himabindu, P. Ravichandra, and J. Annapurna, "Gentamicin
production by micromonospora echinospora (Me-22) in stirred tank
reactor:effectof various parameters:" Journal of basic microbiology
vol.48, 2007, pp. 53-58.
[7] K. Mehrotra, C.K. Mohan, and S. Ranka, S., "Elements of Artificial
Neural Networks", Penram International, Mumbai, 1997
[8] L. Govindarajan, Radial Basis Neural Network Approach to VLE Data
Predictions, M.E., Dissertation, Annamalai University, India, 2002.
[9] Y.C.Chang, C.L. Lee, and T.M. Pan, "Statistical optimization of media
components for the production of Antrodia cinnamomea AC0623 in
submerged cultures", Applied Microbiology and Biotechnology, vol.72,
2006, pp. 654-661.
[10] E.Kristo, C.G. Biliaderis, and N. Tzanetakis, "Modeling of the
acidification process and rheological properties of milk fermented with a
yogurt starter culture using response surface methodology", Food
Chemistry, vol. 83, 2003, pp. 437 - 446.
[11] Q.K. Beg, R.K. Saxena, and R. Gupta, "Kinetic constants determination
for an alkaline protease from Bacillus mojavensis using response surface
methodology", Biotechnol. Bioeng. vol.78, 2002, pp. 289-295.
[12] L.S.T.Lai, C.C. Pan, and B.K. Tzeng, "The influence of medium design
on lovastatin production and pellet formation with a high-producing
mutant of Aspergillus terreus in submerged cultures", Process
Biochemistry, vol.38, 2003, pp. 1317 - 1326.
[13] E.L.Soo, A.B. Salieh and M. Basri, "Response surface methodological
study on lipase-catalyzed synthesis of amino acid surfactants", Process
Biochemistry, vol.39, 2004, pp. 1511 - 1518.
[14] Y.X.Wang, and Z.X. Lu, "Optimization of processing parameters for the
mycelial growth and extracellular polysaccharide production by Boletus
spp. ACCC 50328", Process Biochemistry, vol.40, 2005, pp. 1043 -
1051.
[15] H. Wang, J. Ren, and Y. Zhang, "Use of p-dimethylaminobenzalhyde as
a coloured reagent for determination of gentamycin", Talanta, Vol. 40,
1993, pp.851-853
[16] L. Govindarajan, "Optimal design of reactors", Ph D Thesis, Annamalai
University, India, 2005.
[1] M. J. Weinstein, G.M. Luedemann, E.M. Oden, G.H. Wagman, J.P.
Rosselet, J.A. Marquez, C.T. Coniglio, W. Charney, H.L. Herzog, and J.
Black, "Gentamicin, new antibiotic complex from Micromonospora", J.
Med. Chem., vol. 6, 1963, pp. 463-464.
[2] J. Chu, S. Zhang, Y. Zhuang, J. Chen, and Y. Li, "Factors affecting the
biosynthesis and secretion of gentamicin", Process Biochem., vol.38,
2002, pp. 815-820.
[3] J. Chu, W. Niu, S. Zhang, Y. Zhuang, H. Hu, and Y. Li, "Effect of metal
ions on the binding of gentamicin to the peptidoglycan of
Micromonospora echinospora, Process Biochem, vol.39, 2004, pp.1145-
1150.
[4] M. Himabindu, R. Potumarthi, and A. Kitty, "Enhancement of
gentamicin production by mutugenesis and non nutritional stress
conditions in Micromonospora echinospora". Process Biochem, vol.42,
2007, pp. 1352-1356.
[5] M. Himabindu, P. Ravichandra, and J. Annapurna, "Evaluation of
immobilization conditions for enhanced production of gentamicin in
repeated batch operation by micromonospora echinospora"Int.J.of
chemical Reaction Engineering, vol.5, 2007, pp. 1-13.
[6] M. Himabindu, P. Ravichandra, and J. Annapurna, "Gentamicin
production by micromonospora echinospora (Me-22) in stirred tank
reactor:effectof various parameters:" Journal of basic microbiology
vol.48, 2007, pp. 53-58.
[7] K. Mehrotra, C.K. Mohan, and S. Ranka, S., "Elements of Artificial
Neural Networks", Penram International, Mumbai, 1997
[8] L. Govindarajan, Radial Basis Neural Network Approach to VLE Data
Predictions, M.E., Dissertation, Annamalai University, India, 2002.
[9] Y.C.Chang, C.L. Lee, and T.M. Pan, "Statistical optimization of media
components for the production of Antrodia cinnamomea AC0623 in
submerged cultures", Applied Microbiology and Biotechnology, vol.72,
2006, pp. 654-661.
[10] E.Kristo, C.G. Biliaderis, and N. Tzanetakis, "Modeling of the
acidification process and rheological properties of milk fermented with a
yogurt starter culture using response surface methodology", Food
Chemistry, vol. 83, 2003, pp. 437 - 446.
[11] Q.K. Beg, R.K. Saxena, and R. Gupta, "Kinetic constants determination
for an alkaline protease from Bacillus mojavensis using response surface
methodology", Biotechnol. Bioeng. vol.78, 2002, pp. 289-295.
[12] L.S.T.Lai, C.C. Pan, and B.K. Tzeng, "The influence of medium design
on lovastatin production and pellet formation with a high-producing
mutant of Aspergillus terreus in submerged cultures", Process
Biochemistry, vol.38, 2003, pp. 1317 - 1326.
[13] E.L.Soo, A.B. Salieh and M. Basri, "Response surface methodological
study on lipase-catalyzed synthesis of amino acid surfactants", Process
Biochemistry, vol.39, 2004, pp. 1511 - 1518.
[14] Y.X.Wang, and Z.X. Lu, "Optimization of processing parameters for the
mycelial growth and extracellular polysaccharide production by Boletus
spp. ACCC 50328", Process Biochemistry, vol.40, 2005, pp. 1043 -
1051.
[15] H. Wang, J. Ren, and Y. Zhang, "Use of p-dimethylaminobenzalhyde as
a coloured reagent for determination of gentamycin", Talanta, Vol. 40,
1993, pp.851-853
[16] L. Govindarajan, "Optimal design of reactors", Ph D Thesis, Annamalai
University, India, 2005.
@article{"International Journal of Chemical, Materials and Biomolecular Sciences:57345", author = "M.Rajasimman and S.Subathra", title = "Optimization of Gentamicin Production: Comparison of ANN and RSM Techniques", abstract = "In this work, statistical experimental design was
applied for the optimization of medium constituents for Gentamicin
production by Micromsonospora echinospora subs pallida (MTCC
708) in a batch reactor and the results are compared with the ANN
predicted values. By central composite design, 50 experiments are
carried out for five test variables: Starch, Soya bean meal, K2HPO4,
CaCO3 and FeSO4. The optimum condition was found to be: Starch
(8.9,g/L), Soya bean meal (3.3 g/L), K2HPO4 (0.8 g/L), CaCO3 (4
g/L) and FeSO4 (0.03 g/L). At these optimized conditions, the yield
of gentamicin was found to be 1020 mg/L. The R2 values were found
to be 1 for ANN training set, 0.9953 for ANN test set, and 0.9286 for
RSM.", keywords = "Gentamicin, optimization, Micromonospora
echinospora, ANN, RSM", volume = "3", number = "3", pages = "169-6", }
