Abstract: Agriculture is the backbone of economy of Pakistan
and cotton is the major agricultural export and supreme source of raw
fiber for our textile industry. To combat severe problems of insect
and weed, combination of three genes namely Cry1Ac, Cry2A and
EPSPS genes was transferred in locally cultivated cotton variety
MNH-786 with the use of Agrobacterium mediated genetic
transformation. The present study focused on the molecular screening
of transgenic cotton plants at T3 generation in order to confirm
integration and expression of all three genes (Cry1Ac, Cry2A and
EPSP synthase) into the cotton genome. Initially, glyphosate spray
assay was used for screening of transgenic cotton plants containing
EPSP synthase gene at T3 generation. Transgenic cotton plants which
were healthy and showed no damage on leaves were selected after 07
days of spray. For molecular analysis of transgenic cotton plants in
the laboratory, the genomic DNA of these transgenic cotton plants
were isolated and subjected to amplification of the three genes. Thus,
seventeen out of twenty (Cry1Ac gene), ten out of twenty (Cry2A
gene) and all twenty (EPSP synthase gene) were produced positive
amplification. On the base of PCR amplification, ten transgenic plant
samples were subjected to protein expression analysis through
ELISA. The results showed that eight out of ten plants were actively
expressing the three transgenes. Real-time PCR was also done to
quantify the mRNA expression levels of Cry1Ac and EPSP synthase
gene. Finally, eight plants were confirmed for the presence and active
expression of all three genes at T3 generation.
Abstract: Biofuels production has come forth as a future
technology to combat the problem of depleting fossil fuels. Bio-based
ethanol production from enzymatic lignocellulosic biomass
degradation serves an efficient method and catching the eye of
scientific community. High cost of the enzyme is the major obstacle
in preventing the commercialization of this process. Thus main
objective of the present study was to optimize composition of
medium components for enhancing cellulase production by newly
isolated strain of Bacillus tequilensis. Nineteen factors were taken
into account using statistical Plackett-Burman Design. The significant
variables influencing the cellulose production were further employed
in statistical Response Surface Methodology using Central
Composite Design for maximizing cellulase production. The
optimum medium composition for cellulase production was: peptone
(4.94 g/L), ammonium chloride (4.99 g/L), yeast extract (2.00 g/L),
Tween-20 (0.53 g/L), calcium chloride (0.20 g/L) and cobalt chloride
(0.60 g/L) with pH 7, agitation speed 150 rpm and 72 h incubation at
37oC. Analysis of variance (ANOVA) revealed high coefficient of
determination (R2) of 0.99. Maximum cellulase productivity of 11.5
IU/ml was observed against the model predicted value of 13 IU/ml.
This was found to be optimally active at 60oC and pH 5.5.
Abstract: Load modeling is one of the central functions in
power systems operations. Electricity cannot be stored, which means
that for electric utility, the estimate of the future demand is necessary
in managing the production and purchasing in an economically
reasonable way. A majority of the recently reported approaches are
based on neural network. The attraction of the methods lies in the
assumption that neural networks are able to learn properties of the
load. However, the development of the methods is not finished, and
the lack of comparative results on different model variations is a
problem. This paper presents a new approach in order to predict the
Tunisia daily peak load. The proposed method employs a
computational intelligence scheme based on the Fuzzy neural
network (FNN) and support vector regression (SVR). Experimental
results obtained indicate that our proposed FNN-SVR technique gives
significantly good prediction accuracy compared to some classical
techniques.
Abstract: Neurons in the nervous system communicate with
each other by producing electrical signals called spikes. To
investigate the physiological function of nervous system it is essential
to study the activity of neurons by detecting and sorting spikes in the
recorded signal. In this paper a method is proposed for considering
the spike sorting problem which is based on the nonlinear modeling
of spikes using exponential autoregressive model. The genetic
algorithm is utilized for model parameter estimation. In this regard
some selected model coefficients are used as features for sorting
purposes. For optimal selection of model coefficients, self-organizing
feature map is used. The results show that modeling of spikes with
nonlinear autoregressive model outperforms its linear counterpart.
Also the extracted features based on the coefficients of exponential
autoregressive model are better than wavelet based extracted features
and get more compact and well-separated clusters. In the case of
spikes different in small-scale structures where principal component
analysis fails to get separated clouds in the feature space, the
proposed method can obtain well-separated cluster which removes
the necessity of applying complex classifiers.
Abstract: PLA emerged as a promising polymer because of its
property as a compostable, biodegradable thermoplastic made from
renewable sources. PLA can be polymerized from monomers
(Lactide or Lactic acid) obtained by fermentation processes from
renewable sources such as corn starch or sugarcane. For PLA
synthesis, ring opening polymerization (ROP) of Lactide monomer is
one of the preferred methods. In the literature, the technique mainly
developed for ROP of PLA is based on metal/bimetallic catalyst (Sn,
Zn and Al) or other organic catalysts in suitable solvent. However,
the PLA synthesized using such catalysts may contain trace elements
of the catalyst which may cause toxicity. This work estimated the
usefulness and drawbacks of using different catalysts as well as effect
of alternative energies and future aspects for PLA production.
Abstract: The main aim of this article is to present a balance of the decade of Polish air transport market in the European Union having taking into account selected entities of the aviation market. This article analyzes the functioning of the Polish air transport market after the Polish accession to the European Union. During the study two main areas were pointed: shipping activity and activity of the airports. The most important benefits of integration and the benefits of introducing of the open sky policy were indicated. The last part of the article presents the perspectives of development of air traffic.
Abstract: Thin ZnO films are deposited on glass substrates via
sol–gel method and dip-coating. The films are prepared from zinc
acetate dehydrate as a starting reagent. After that the as-prepared
ZnO sol is aged for different periods (0, 1, 3, 5, 10, 15 and 30 days).
Nanocrystalline thin films are deposited from various sols. The
effect ZnO sols aging time on the structural and photocatalytic
properties of the films is studied. The films surface is studied by
Scanning Electron Microscopy. The effect of the aging time of the
starting solution is studied in the photocatalytic degradation of
Reactive Black 5 (RB5) by UV-vis spectroscopy. The experiments
are conducted upon UV-light illumination and in complete darkness.
The variation of the absorption spectra shows the degradation of RB5
dissolved in water, as a result of the reaction, occurring on the surface
of the films and promoted by UV irradiation. The initial
concentrations of dye (5, 10 and 20 ppm) and the effect of the aging
time are varied during the experiments. The results show, that the
increasing aging time of starting solution with respect to ZnO
generally promotes photocatalytic activity. The thin films obtained
from ZnO sol, which is aged 30 days have best photocatalytic
degradation of the dye (97,22%) in comparison with the freshly
prepared ones (65,92%). The samples and photocatalytic
experimental results are reproducible. Nevertheless, all films exhibit
a substantial activity in both UV light and darkness, which is
promising for the development of new ZnO photocatalysts by sol-gel
method.
Abstract: The temperature dependence of wettability (wetting
angle, Θ (T)) for Ag-based melts on graphite and Al2O3 substrates is
compared. Typical alloying effects are found, as the Ag host metal is
gradually replaced by various metallic elements. The essence of
alloying lies in the change of the electron/atom (e/a) ratio. This ratio
is also manifested in the shift of wetting angles on the same substrate.
Nevertheless, the effects are partially smeared by other
(metallurgical) factors, like the interaction between the oxygenalloying
elements and by the graphite substrate-oxygen interaction. In
contrast, such effects are not pronounced in the case of Al2O3
substrates. As a consequence, Θ(T) exhibits an opposite trend in the
case of two substrates. Crossovers of the Θ(T) curves were often
found. The positions of crossovers depend on the chemical character
and concentration of solute atoms. Segregation and epitaxial texture
formation after solidification were also observed in certain alloy
drops, especially in high concentration range. This phenomenon is
not yet explained in every detail.
Abstract: The well been of human beings on construction site is
very important, many man power had been lost through accidents
which kills or make workers physically unfit to carry out construction
activities, these in turn have multiple effects on the whole economy.
Thus it is necessary to put all safety items and regulations in place
before construction activities can commence. This study was carried
out in Ondo state of Nigeria to known and analyse the state of health
and safety of construction workers in the state. The study was done
using first hand observation method, 50 construction project sites
were visited in 10 major towns of Ondo state, questionnaires were
distributed and the results were analysed. The result show that
construction workers are being exposed to a lot of construction site
hazards due to lack of inadequate safety programmes and nonprovision
of appropriate safety materials for workers on site. From the
data gotten for each site visited and the statistical analysis, it can be
concluded that occurrence of accident on construction sites depends
significantly on the available safety facilities on the sites. The result of
the regression statistics show that the level of significant of the
dependence of occurrence of accident on the availability of safety
items on site is 0.0362 which is less than 0.05 maximum significant
level required. Therefore a vital way of sustaining our building
strategy is by given a detail attention to provision of adequate health
and safety items on construction sites which will reduce the
occurrence of accident, loss of man power and death of skilled
workers among others.
Abstract: The investigation in the present paper is to obtain
certain types of relations for the well known hypergeometric functions
by employing the technique of fractional derivative and integral.
Abstract: The tombolo of Giens is located in the town of Hyères
(France). We recall the history of coastal erosion, and prominent
factors affecting the evolution of the western tombolo. We then
discuss the possibility of stabilizing the western tombolo. Our
argumentation relies on a coupled model integrating swells, currents,
water levels and sediment transport. We present the conclusions of
the simulations of various scenarios, including pre-existing
propositions from coastal engineering offices. We conclude that
beach replenishment seems to be necessary but not sufficient for the
stabilization of the beach. Breakwaters reveal effective particularly in
the most exposed northern area. Some solutions fulfill conditions so
as to be elected as satisfactory. We give a comparative analysis of the
efficiency of 14 alternatives for the protection of the tombolo.
Abstract: The use of technology in the classroom is an issue that
is constantly evolving. Digital age students learn differently than their
teachers did, so now the teacher should be constantly evolving their
methods and teaching techniques to be more in touch with the
student. In this paper a case study presents how were used some of
these technologies by accompanying a classroom course, this in order
to provide students with a different and innovative experience as their
teacher usually presented the activities to develop. As students
worked in the various activities, they increased their digital skills by
employing unknown tools that helped them in their professional
training. The twenty-first century teacher should consider the use of
Information and Communication Technologies in the classroom
thinking in skills that students of the digital age should possess. It
also takes a brief look at the history of distance education and it is
also highlighted the importance of integrating technology as part of
the student's training.
Abstract: A novel hybrid model of the lumbar spine, allowing
fast static and dynamic simulations of the disc pressure
and the spine mobility, is introduced in this work. Our
contribution is to combine rigid bodies, deformable finite
elements, articular constraints, and springs into a unique model
of the spine. Each vertebra is represented by a rigid body
controlling a surface mesh to model contacts on the facet
joints and the spinous process. The discs are modeled using
a heterogeneous tetrahedral finite element model. The facet
joints are represented as elastic joints with six degrees of
freedom, while the ligaments are modeled using non-linear
one-dimensional elastic elements. The challenge we tackle
is to make these different models efficiently interact while
respecting the principles of Anatomy and Mechanics.
The mobility, the intradiscal pressure, the facet joint force and
the instantaneous center of rotation of the lumbar spine are
validated against the experimental and theoretical results of
the literature on flexion, extension, lateral bending as well as
axial rotation.
Our hybrid model greatly simplifies the modeling task and
dramatically accelerates the simulation of pressure within the
discs, as well as the evaluation of the range of motion and the
instantaneous centers of rotation, without penalizing precision.
These results suggest that for some types of biomechanical
simulations, simplified models allow far easier modeling and
faster simulations compared to usual full-FEM approaches
without any loss of accuracy.
Abstract: The purpose of this study is to forecast the influences
of information and communication technology (ICT) on the structural
changes of Japanese economies. In this study, input-output (IO) and
statistical approaches are used as analysis instruments. More
specifically, this study employs Leontief IO coefficients and
constrained multivariate regression (CMR) model in order to achieve
the purpose. The periods of initial and forecast in this study are 2005
and 2015, respectively. In this study, ICT is represented by ICT capital
stocks. This study conducts two levels of analysis, namely macro and
micro. The results of macro level analysis show that the dynamics of
Japanese economies on the forecast period, relative to the initial period,
are not so high. We focus on (1) commerce, (2) business services and
office supplies, and (3) personal services sectors when conducting the
analysis of the micro level. Further, we analyze its specific IO
coefficients when doing this analysis. The results of the analysis
explain that ICT gives a strong influence on the changes of these
coefficients from initial to forecast periods.
Abstract: The aim of this paper is to give an assessment of
environmental effects of IPPC permit conditions of installations that
are in specific territory with high concentration of industrial
activities.
The IPPC permit is the permit that each operator should hold to
operate the installation as stated by the directive 2010/75/UE on
industrial emissions (integrated pollution prevention and control),
known as IED (Industrial Emissions Directive).
The IPPC permit includes all the measures necessary to achieve a
high level of protection of the environment as a whole, also defining
the monitoring requirements as measurement methodology,
frequency and evaluation procedure. The emissions monitoring of a
specific plant may also give indications of the contribution of these
emissions on the air quality of a definite area.
So, it is clear that the IPPC permits are important tools both to
improve the environmental framework and to achieve the air quality
standards, assisting to assess the possible industrial sources
contributions to air pollution.
Abstract: The present paper summarizes the analysis of the
request for consultation of information and data on industrial
emissions made publicly available on the web site of the Ministry of
Environment, Land and Sea on integrated pollution prevention and
control from large industrial installations, the so called “AIA Portal”.
As a matter of fact, a huge amount of information on national
industrial plants is already available on internet, although it is usually
proposed as textual documentation or images.
Thus, it is not possible to access all the relevant information
through interoperability systems and also to retrieval relevant
information for decision making purposes as well as rising of
awareness on environmental issue.
Moreover, since in Italy the number of institutional and private
subjects involved in the management of the public information on
industrial emissions is substantial, the access to the information is
provided on internet web sites according to different criteria; thus, at
present it is not structurally homogeneous and comparable.
To overcome the mentioned difficulties in the case of the
Coordinating Committee for the implementation of the Agreement
for the industrial area in Taranto and Statte, operating before the
IPPC permit granting procedures of the relevant installation located
in the area, a big effort was devoted to elaborate and to validate data
and information on characterization of soil, ground water aquifer and
coastal sea at disposal of different subjects to derive a global
perspective for decision making purposes. Thus, the present paper
also focuses on main outcomes matured during such experience.
Abstract: Verification and Validation of Simulated Process
Model is the most important phase of the simulator life cycle.
Evaluation of simulated process models based on Verification and
Validation techniques checks the closeness of each component model
(in a simulated network) with the real system/process with respect to
dynamic behaviour under steady state and transient conditions. The
process of Verification and Validation helps in qualifying the process
simulator for the intended purpose whether it is for providing
comprehensive training or design verification. In general, model
verification is carried out by comparison of simulated component
characteristics with the original requirement to ensure that each step
in the model development process completely incorporates all the
design requirements. Validation testing is performed by comparing
the simulated process parameters to the actual plant process
parameters either in standalone mode or integrated mode.
A Full Scope Replica Operator Training Simulator for PFBR -
Prototype Fast Breeder Reactor has been developed at IGCAR,
Kalpakkam, INDIA named KALBR-SIM (Kalpakkam Breeder
Reactor Simulator) where in the main participants are
engineers/experts belonging to Modeling Team, Process Design and
Instrumentation & Control design team. This paper discusses about
the Verification and Validation process in general, the evaluation
procedure adopted for PFBR operator training Simulator, the
methodology followed for verifying the models, the reference
documents and standards used etc. It details out the importance of
internal validation by design experts, subsequent validation by
external agency consisting of experts from various fields, model
improvement by tuning based on expert’s comments, final
qualification of the simulator for the intended purpose and the
difficulties faced while co-coordinating various activities.
Abstract: The progress of industry integrated circuits in recent
years has been pushed by continuous miniaturization of transistors.
With the reduction of dimensions of components at 0.1 micron and
below, new physical effects come into play as the standard simulators
of two dimensions (2D) do not consider. In fact the third dimension
comes into play because the transverse and longitudinal dimensions
of the components are of the same order of magnitude. To describe
the operation of such components with greater fidelity, we must
refine simulation tools and adapted to take into account these
phenomena. After an analytical study of the static characteristics of
the component, according to the different operating modes, a
numerical simulation is performed of field-effect transistor with
submicron gate MESFET GaInP. The influence of the dimensions of
the gate length is studied. The results are used to determine the
optimal geometric and physical parameters of the component for their
specific applications and uses.
Abstract: The design and plantwide control of an integrated
plant where the endothermic 1,4-butanediol dehydrogenation and the
exothermic furfural hydrogenation is simultaneously performed in a
single reactor is studied. The reactions can be carried out in an
adiabatic reactor using small hydrogen excess and with reduced
parameter sensitivity. The plant is robust and flexible enough to
allow different production rates of γ-butyrolactone and 2-methyl
furan, keeping high product purities. Rigorous steady state and
dynamic simulations performed in AspenPlus and AspenDynamics to
support the conclusions.
Abstract: The increasing demand of gallium, indium and
rare-earth elements for the production of electronics, e.g. solid
state-lighting, photovoltaics, integrated circuits, and liquid crystal
displays, will exceed the world-wide supply according to current
forecasts. Recycling systems to reclaim these materials are not yet in
place, which challenges the sustainability of these technologies. This
paper proposes a multispectral imaging system as a basis for a vision
based recognition system for valuable components of electronics
waste. Multispectral images intend to enhance the contrast of images
of printed circuit boards (single components, as well as labels) for
further analysis, such as optical character recognition and entire
printed circuit board recognition. The results show, that a higher
contrast is achieved in the near infrared compared to ultraviolett and
visible light.