Abstract: Experiments were carried out at the Latvia State
Institute of Fruit-Growing in 2011. Fresh-cut minimally processed
apple and pear mixed salad were packed by passive modified
atmosphere (MAP) in PP containers, which were hermetically sealed
by breathable conventional BOPP PropafreshTM P2GAF, and Amcor
Agrifresh films. Biodegradable NatureFlexTM NVS INNOVIA Films
and VC999 BioPack PLA films coated with a barrier of pure silicon
oxide (SiOx) were used to compare the fresh-cut produce quality
with this packed in conventional packaging films. Samples were cold
stored at temperature +4.0±0.5 °C up to 10 days. The quality of salad
was evaluated by physicochemical properties – weight losses,
moisture, firmness, the effect of packaging modes on the colour,
dynamics in headspace atmosphere concentration (CO2 and O2),
titratable acidity values, as well as by microbiological contamination
(yeasts, moulds and total bacteria count) of salads, analyzing before
packaging and after 2, 4, 6, 8, and 10 storage days.
Abstract: The most common domestic birds live in Turkey are: crows (Corvus corone), pigeons (Columba livia), sparrows (Passer domesticus), starlings (Sturnus vulgaris) and blackbirds (Turdus merula). These birds give damage to the agricultural areas and make dirty the human life areas. In order to send away these birds, some different materials and methods such as chemicals, treatments, colored lights, flash and audible scarers are used. It is possible to see many studies about chemical methods in the literatures. However there is not enough works regarding audible bird scarers are reported in the literature. Therefore, a solar powered bird scarer was designed, manufactured and tested in this experimental investigation. Firstly, to understand the sensitive level of these domestic birds against to the audible scarer, many series preliminary studies were conducted. These studies showed that crows are the most resistant against to the audible bird scarer when compared with pigeons, sparrows, starlings and blackbirds. Therefore the solar powered audible bird scarer was tested on crows. The scarer was tested about one month during April- May, 2007. 18 different common known predators- sounds (voices or calls) of domestic birds from Falcon (Falco eleonorae), Falcon (Buteo lagopus), Eagle (Aquila chrysaetos), Montagu-s harrier (Circus pygargus) and Owl (Glaucidium passerinum) were selected for test of the scarer. It was seen from the results that the reaction of the birds was changed depending on the predators- sound type, camouflage of the scarer, sound quality and volume, loudspeaker play and pause periods in one application. In addition, it was also seen that the sound from Falcon (Buteo lagopus) was most effective on crows and the scarer was enough efficient.
Abstract: To study the effect of suitable methods for
propagation of True Potato Seed (TPS) progenies, transplant and
selection of the best progenies, a factorial experiment base on a
randomized complete block design was carried out in the research
field of Sahneh region, Kermanshah, Iran during 2009-2010. Five
selective progenies from CIP (International Potato Center) including
CIP.994013, CIP.994002, CIP.994014, CIP.888006, and
CIP.994001 and two transplant preparation methods (Paper pot
preparation for mechanical cultivation and preparation in transplant
trays for manual cultivation) were studied in three replications.
Results showed that different progenies had no significant effect on
plant height (cm) and tuber yield (t ha-1), whereas had a significant
effect on number of tubers per unit area (m2). There was significant
difference between transplant preparation methods for plant height
and tuber yield. The interaction effect of progenies and transplant
preparation method was not significant for these traits. CIP.888006
progeny and paper pot preparation method produced the highest
tuber yields. Also CIP.994002 and CIP.994014 progenies considered
as the best progenies under paper pot preparation method due to high
yields.
Abstract: Cytogenetic analysis still remains the gold standard method for prenatal diagnosis of trisomy 21 (Down syndrome, DS). Nevertheless, the conventional cytogenetic analysis needs live cultured cells and is too time-consuming for clinical application. In contrast, molecular methods such as FISH, QF-PCR, MLPA and quantitative Real-time PCR are rapid assays with results available in 24h. In the present study, we have successfully used a novel MGB TaqMan probe-based real time PCR assay for rapid diagnosis of trisomy 21 status in Down syndrome samples. We have also compared the results of this molecular method with corresponding results obtained by the cytogenetic analysis. Blood samples obtained from DS patients (n=25) and normal controls (n=20) were tested by quantitative Real-time PCR in parallel to standard G-banding analysis. Genomic DNA was extracted from peripheral blood lymphocytes. A high precision TaqMan probe quantitative Real-time PCR assay was developed to determine the gene dosage of DSCAM (target gene on 21q22.2) relative to PMP22 (reference gene on 17p11.2). The DSCAM/PMP22 ratio was calculated according to the formula; ratio=2 -ΔΔCT. The quantitative Real-time PCR was able to distinguish between trisomy 21 samples and normal controls with the gene ratios of 1.49±0.13 and 1.03±0.04 respectively (p value
Abstract: This paper presents a novel method for inferring the
odor based on neural activities observed from rats- main olfactory
bulbs. Multi-channel extra-cellular single unit recordings were done
by micro-wire electrodes (tungsten, 50μm, 32 channels) implanted in
the mitral/tufted cell layers of the main olfactory bulb of anesthetized
rats to obtain neural responses to various odors. Neural response
as a key feature was measured by substraction of neural firing rate
before stimulus from after. For odor inference, we have developed a
decoding method based on the maximum likelihood (ML) estimation.
The results have shown that the average decoding accuracy is about
100.0%, 96.0%, 84.0%, and 100.0% with four rats, respectively. This
work has profound implications for a novel brain-machine interface
system for odor inference.
Abstract: The genus Fumaria L. (Papaveraceae) in Iran
comprises 8 species with a vast medicinal use in Asian folk
medicine. These herbs are considered to be useful in the
treatment of gastrointestinal disease and skin disorders.
Antioxidant activities of alkaloids and phenolic extracts of
these species had been studied previously. These species are:
F. officinalis, F. parviflora, F. asepala, F. densiflora, F.
schleicheri, F. vaillantii and F. indica. More than 50
populations of Fumaria species were sampled from nature. In
this study different fatty acids are extracted. Their picks were
recorded by GC technique. This species contain some kind of
fatty acids with antioxidant effects. A part of these lipids are
phospholipids. As these are unsaturated fatty acids they may
have industrial use as natural additive to cosmetics, dermal
and oral medicines. The presences of different materials are
discussed. Our studies for antioxidant effects of these
substances are continued.
Abstract: Lighvan cheese is basically made from sheep milk in
the area of Sahand mountainside which is located in the North West
of Iran. The main objective of this study was to investigate the effect
of enterococci isolated from traditional Lighvan cheese on the quality
of Iranian UF white during ripening. The experimental design was
split plot based on randomized complete blocks, main plots were four
types of starters and subplots were different ripening durations.
Addition of Enterococcus spp. did not significantly (P
Abstract: Pattern matching is one of the fundamental applications in molecular biology. Searching DNA related data is a common activity for molecular biologists. In this paper we explore the applicability of a new pattern matching technique called Index based Forward Backward Multiple Pattern Matching algorithm(IFBMPM), for DNA Sequences. Our approach avoids unnecessary comparisons in the DNA Sequence due to this; the number of comparisons of the proposed algorithm is very less compared to other existing popular methods. The number of comparisons rapidly decreases and execution time decreases accordingly and shows better performance.
Abstract: Oxidative stress and overwhelming free radicals
associated with diabetes mellitus are likely to be linked with
development of certain complication such as retinopathy,
nephropathy and neuropathy. Treatment of diabetic subjects with
antioxidant may be of advantage in attenuating these complications.
Olive leaf (Oleaeuropaea), has been endowed with many beneficial
and health promoting properties mostly linked to its antioxidant
activity. This study aimed to evaluate the significance of
supplementation of Olive leaves extract (OLE) in reducing oxidative
stress, hyperglycemia and hyperlipidemia in Sterptozotocin (STZ)-
induced diabetic rats. After induction of diabetes, a significant rise in
plasma glucose, lipid profiles except High density lipoproteincholestrol
(HDLc), malondialdehyde (MDA) and significant decrease
of plasma insulin, HDLc and Plasma reduced glutathione GSH as
well as alteration in enzymatic antioxidants was observed in all
diabetic animals. During treatment of diabetic rats with 0.5g/kg body
weight of Olive leaves extract (OLE) the levels of plasma (MDA)
,(GSH), insulin, lipid profiles along with blood glucose and
erythrocyte enzymatic antioxidant enzymes were significantly
restored to establish values that were not different from normal
control rats. Untreated diabetic rats on the other hand demonstrated
persistent alterations in the oxidative stress marker (MDA), blood
glucose, insulin, lipid profiles and the antioxidant parameters. These
results demonstrate that OLE may be of advantage in inhibiting
hyperglycemia, hyperlipidemia and oxidative stress induced by
diabetes and suggest that administration of OLE may be helpful in
the prevention or at least reduced of diabetic complications
associated with oxidative stress.
Abstract: Gastric ulceration is a discontinuity in gastric mucosa, usually occurs due to imbalance between the gastric mucosal protective factors, that is called gastric mucosal barrier, and the aggressive factors, to which the mucosa is exposed. This study was carried out on sixty male Sprague-Dowely rats (12- 16 weeks old) allocated into two groups. The first control group and the second Gastric lesion group which induced by oral administration of a single daily dose of aspirin at a dose of 300 mg/kg body weight for 7 consecutive-days (6% aspirin solution will be prepared and each rat will be given 5 ml of that solution/kg body weight). Blood is collected 1, 2 and 3 weeks after induction of gastric ulceration. Significant increase in serum copper, nitric oxide, and prostaglandin E2 all over the period of experiment. Significant decrease in erythrocyte superoxide dismutase (t-SOD) activities, serum (calcium, phosphorus, glucose and insulin) levels. Non-significant changes in serum sodium and potassium levels are obtained.
Abstract: Two optimized strategies were successfully established
to develop biomolecule-based magnetic nanoassemblies.
Streptavidin-coated and amine-coated magnetic nanoparticles were
chosen as model scaffolds onto which double-stranded DNA and
human immunoglobulin G were specifically conjugated in succession,
using biotin-streptavidin interaction or covalent cross-linkers. The
success of this study opens the prospect of developing selective and
sensitive nanoparticle-based structures for diagnostics or drug
delivery.
Abstract: The in vitro culture procedure of purple nutsedge
(Cyperus rotundus L.) for multiple shoot induction and tuber
formation was established. Multiple shoots were significantly
induced from a single shoot of about 0.5 – 0.8 cm long, on Murashige
and Skoog (MS) medium supplemented with 4.44 μM 6-
benzyladinine (BA) alone or in combination with 2.85 μM 1-
indoleacetic acid (IAA), providing 17.6 and 15.3 shoots per explant
with 31.2 and 27.5 leaves per explant, respectively, within 6 weeks of
culturing. Moreover, MS medium supplemented with 4.44 μM BA
and 2.85 μM IAA was suitable for tuber induction, obtaining 5.9
tubers with 3.4 rhizomes per explant. In combination with ancymidol
and higher concentration of sucrose, 11.1 μM BA and 60 g/L sucrose
or 11.1 μM BA, 7.8 μM ancymidol and 60 g/L sucrose induced 3.5
tubers with 1.6 rhizomes or 3.5 tubers without rhizome, respectively.
However, MS medium containing 3.9 or 7.8 μM ancymidol in
combination with either 60 or 80 g/L sucrose enchanced significant
root formation at 20.9 – 23.6 roots per explant.
Abstract: In the course of the present work, plain (nonencapsulated)
and microencapsulated polyphenols were produced
using olive mill wastewater (OMW) as raw material, in order to be
used for enrichment of yogurt and dairy products. The OMW was
first clarified by using membrane technology and subsequently the
contained poly-phenols were isolated by adsorption-desorption
technique using selective macro-porous resins and finally recovered
in dry form after been processed by RO membrane technique
followed by freeze drying. Moreover, the polyphenols were
encapsulated in modified starch by freeze drying in order to mask the
color and bitterness effect and improve their functionality. The two
products were used successfully as additives in yogurt preparations
and the produced products were acceptable by the consumers and
presented with certain advantage to the plain yogurt. For the herein
proposed production scheme a patent application was already
submitted.
Abstract: Agropyron cristatum L. Gaertn. is a native grass of
semiarid region in Iran which is quit resistant to cool and drought
climate and withstand heavy grazing. This species has close
phylogenetic relationship with Triticum and Hordeum. In this
research, the effect of seven different concentrations of growth
regulator 2,4-D on callus production and somatic embryogenesis of
A. cristatum was investigated on Murashige and Skoog medium. The
results showed that the rate of callus, embryo and neomorph were
highest in 1 mg L-1 2,4-D. Callus production was increased in 1 mg
L-1 2,4-D but dramatically decreased at 5.5 and 9 mg L-1 2,4-D. The
somatic embryos were observed at 1 and 4 mg L-1 2,4-D but matured
embryos and plantlet were only occurred at 1 mg L-1 2,4-D. There
were significant differences between 1 mg L-1 2,4-D and other
treatments for producing globular and torpedo embryos, plantlet,
rooted callus and number of roots (p
Abstract: The experimental design was 4 x 5 factorial with three
replications in fully controlled research greenhouse in Department of
Soil Sciences and Plant Nutrition, Faculty of Agriculture, University
of Selcuk in the year of 2009. Determination of tolerant chickpea
genotypes to drought was made in the research. Additionally,
sophisticated effects of drought on plant growth and development,
biochemical and physical properties or physical defense mechanisms
were presented. According to the results, the primary genotypes were
Ilgın YP (0.0063 g/gh) for leaf water capacity, 22235 70.44(%) for
relative water content, 22159 (82.47%) for real water content,
22159 (5.03 mg/l) for chlorophyll a+b, Ilgın YP (125.89 nmol
H2O2.dak-1/ mg protein-1) for peroxidase, Yunak YP (769.67
unit/ mg protein-1) for superoxide dismutase, Seydişehir YP
(16.74 μg.TA-1) for proline, Gökçe (80.01 nmol H2O2.dak-1/ mg
protein-1) for catalase. Consequently, all the genotypes
increased their enzyme activity depending on the increasing of
drought stress consider with the effects of drought stress on leaf
enzyme activity. Chickpea genotypes are increasing enzyme
activity against to drought stress.
Abstract: This work was conducted to improve the level of
resistant starch (RS) in a rice noodle using unripe banana flour and to
investigate the effect of substitution of unripe banana flour for rice
flour on the physical properties of rice noodle. In order to prepare
rice noodles, the unripe banana flour were replaced the rice flour
with different degrees of substitutions including 0, 20, 40, 60, 80, and
100%. The results indicated that substitution of unripe banana flour
was significantly affected the viscosity properties of noodle flour,
color, cooking loss, RS and total starch content of noodle. It was
found that the noodle prepared from 100% unripe banana indicated
the greatest changes on the viscosity properties and color profiles. It
also showed the highest values of cooking loss (2.53%), tensile
strength (129.03%), and RS content (13.15%).
Abstract: Sixteen selected deep-sea fish obtained from Southern
Java Ocean and Western Sumatra Ocean was analyzed to determine
its proximate, fatty acid and mineral composition. The moisture
content was ranged from 64.38 to 86.04 %, ash from 0.17 to 0.69 %,
the fat content was 1.54 – 13.30 % while the protein content varied
from 15.84 to 23.60%. Among the fatty acids, oleic acid and palmitic
acid was the dominant MUFA and SFA. Linoleic acid was the
highest PUFA found at the selected deep-sea fish. Phospor was the
highest macroelement concentration on selected deep-sea fish,
followed by K, Ca, Mg and Iod, Fe and Zn among microelement. The
trace concentration was found at Se microelement.
Abstract: The alternative technique for sterilization of culture
medium to replace autoclaving was carried out. For sterilization of
culture medium without autoclaving, some commercial pure essential
oils, bergamot oil, betel oil, cinnamon oil, lavender oil and turmeric
oil, were tested alone or in combinations with some disinfectants,
10% povidone-iodine and 2% iodine + 2.4% potassium iodide. Each
essential oil or combination was added to 25-mL Murashige and
Skoog (MS) medium before medium was solidified in a 120-mL
container, kept for 2 weeks before evaluating sterile conditions.
Treated media, supplemented with essential oils, were compared to
control medium, autoclaved at 121 degree Celsius for 15 min. In
vitro sterile conditions were found 20 – 100% from these treated
media compared to 100% sterile condition from autoclaved medium.
Treated media obtained 100% sterile conditions were chosen for
culturing chrysanthemum shoots. It was found that 10% povidoneiodine
in combination with cinnamon oil (3:1) and 2% iodine + 2.4%
potassium iodide in combination with lavender oil (1:3) at the
concentration of 36 3L/25 mL medium provided the promising
growth of shoot explants.
Abstract: Genome profiling (GP), a genotype based technology, which exploits random PCR and temperature gradient gel electrophoresis, has been successful in identification/classification of organisms. In this technology, spiddos (Species identification dots) and PaSS (Pattern similarity score) were employed for measuring the closeness (or distance) between genomes. Based on the closeness (PaSS), we can buildup phylogenetic trees of the organisms. We noticed that the topology of the tree is rather robust against the experimental fluctuation conveyed by spiddos. This fact was confirmed quantitatively in this study by computer-simulation, providing the limit of the reliability of this highly powerful methodology. As a result, we could demonstrate the effectiveness of the GP approach for identification/classification of organisms.
Abstract: Statistical analysis of electrophysiological recordings
obtained under, e.g. tactile, stimulation frequently suggests participation
in the network dynamics of experimentally unobserved “hidden"
neurons. Such interneurons making synapses to experimentally
recorded neurons may strongly alter their dynamical responses to
the stimuli. We propose a mathematical method that formalizes this
possibility and provides an algorithm for inferring on the presence
and dynamics of hidden neurons based on fitting of the experimental
data to spike trains generated by the network model. The model
makes use of Integrate and Fire neurons “chemically" coupled
through exponentially decaying synaptic currents. We test the method
on simulated data and also provide an example of its application to
the experimental recording from the Dorsal Column Nuclei neurons
of the rat under tactile stimulation of a hind limb.